Stefano Mazzoleni | Università degli Studi di Napoli "Federico II" (original) (raw)
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Papers by Stefano Mazzoleni
Microbial Cell, Dec 3, 2023
Forest Ecology and Management, Jul 1, 2023
Community Ecology, Aug 1, 2019
Community Ecology, Dec 1, 2017
Community Ecology, Dec 1, 2015
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microor... more The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
Scientific Reports, Nov 13, 2023
Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2012
Frontiers research topics, 2017
Journal of Soil Science and Plant Nutrition, Mar 15, 2022
Frontiers in Environmental Science, Aug 29, 2016
Phytocoenologia, Apr 19, 1993
A study of bryophyte population characters in relation to vascular vegetation patterns was done i... more A study of bryophyte population characters in relation to vascular vegetation patterns was done in the island of Capri, southern Italy. 118 phytosociological samples were analyzed by multivariate methods of classification and ordination. 15 interpretable clusters of releves were found, clearly defined by differential bryophyte species, growth forms and ecological characters, whose trends were related to vegetation disturbance patterns
Phytochemistry Reviews, Nov 9, 2021
Microbial Cell, Dec 3, 2023
Forest Ecology and Management, Jul 1, 2023
Community Ecology, Aug 1, 2019
Community Ecology, Dec 1, 2017
Community Ecology, Dec 1, 2015
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microor... more The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
Scientific Reports, Nov 13, 2023
Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2012
Frontiers research topics, 2017
Journal of Soil Science and Plant Nutrition, Mar 15, 2022
Frontiers in Environmental Science, Aug 29, 2016
Phytocoenologia, Apr 19, 1993
A study of bryophyte population characters in relation to vascular vegetation patterns was done i... more A study of bryophyte population characters in relation to vascular vegetation patterns was done in the island of Capri, southern Italy. 118 phytosociological samples were analyzed by multivariate methods of classification and ordination. 15 interpretable clusters of releves were found, clearly defined by differential bryophyte species, growth forms and ecological characters, whose trends were related to vegetation disturbance patterns
Phytochemistry Reviews, Nov 9, 2021
PiroPinus was developed in Portugal as a decision support-tool to prescribed burning use in Pinus... more PiroPinus was developed in Portugal as a decision support-tool to prescribed burning use in Pinus pinaster. Although tailored for site-specific conditions, it is empirically-based and so its performance outside the environmental range of development is uncertain. The objective of the present study is to test PiroPinus 2.2 utilities to define prescriptions, implement and evaluate prescribed burning for fire hazard reduction in Pinus halepensis plantations. The study site was located in the Cilento and Vallo di Diano National Park, one of the most fire-prone areas of Southern Italy. The experimental design consisted of 3 burn plots (BU), 0.2 ha in size, and 2 controls (CO). In all plots the plantation presented high fire risk. Pre-burn surface fuels (5.2±1.3 t ha-1 of litter, and flammable Ampelodesmos mauritanicus) and elevated fuels (dominated by Erica arborea) presented horizontal and vertical continuity. Objectives for the burn were: (1) reduce surface fuels; (2) create vertical discontinuity. PiroPinus was used as a reference guide to define burning windows. All plots were burned in May 2009. Most of burning parameters fell within the PiroPinus prescriptions. Fire behaviour was assessed with a microplot scale approach. Observed average surface litter moisture was 17%; rate of spread 0.22 ± 0.06 m min-1; flame length range 0.2-1.0 m; fireline intensity 52 ± 10 kW m-1. PiroPinus provided an accurate estimate of observed values: 17%, 0.25 m min-1, 0.5 m and 48 kW m-1 respectively. Prescribed burning objectives were achieved to some extent. According to the PiroPinus fire interpretation table the burn was conducted at the upper limit of moisture conditions. In fact, in summer 2009 surface fuels in BU were reduced only in part. Major changes were observed in elevated fuels whose cover decreased from 58% to 3%; the vertical continuity was remarkably reduced. Finally, PiroPinus was used to model post-treatment fire behaviour under different weather conditions. It predicted a remarkable mitigation of fire behaviour in BU vs. CO for all weather scenarios. Under the 97.5th scenario, simulated rate of spread, flame length and intensity in BU were respectively 75%, 31% and 12% of the CO values. PiroPinus resulted a useful tool to support prescribed burning in Pinus halepensis plantations of the Cilento Park. Despite constraints (research issues to address are outlined), this study showed that experimental data can be used to improve the performance of PiroPinus, extending its use as a reference guide for Mediterranean pine forests other than Pinus pinaster.