Teresa Capriglione | Università degli Studi di Napoli "Federico II" (original) (raw)

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Papers by Teresa Capriglione

Research paper thumbnail of Genome size evolution in vertebrates: Trends and constraints

Comparative Biochemistry and Physiology Part B Comparative Biochemistry, Feb 1, 1989

Research paper thumbnail of New Data on Karyotype of Some Scincidae

Caryologia, 1987

The karyotypes of two scincid species (Eumeces inexpectatus and Sphenops sphenopsi/ormis) have be... more The karyotypes of two scincid species (Eumeces inexpectatus and Sphenops sphenopsi/ormis) have been studied. E. inexpectatus shows 12 biarmed macrochromosomes and 14 microchromosomes. This karyotype is similar to that observed in some species of the same genus. S. sphenopsi/ormis shows, instead, 16 biarmed and 4 uniarmed macrochromosomes and 8 microchromosomes. This karyotype is very similar to that possessed by the Chalcides species studied so far. It is suggested that S. sphenopsiformis is closer to the genus Chalcides than what has been supposed previously. It is also supposed that the karyotypes of S. sphenopsiformis and C. chalcides might be derived, through a series of pericentric inversions, from that of C. ocellatus tiligugu, in which all the macrochromosomes are biarmed.

Research paper thumbnail of The karyology of Mediterranean lacertid lizards

Research paper thumbnail of Results and Perspectives in the Study of Scincomorph Karyology

Research paper thumbnail of Karyology of the Antarctic scallop Adamussium colbecki , with some comments on the karyological evolution of pectinids

Genetica, Jun 1, 2006

Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and comp... more Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n=38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two telocentrics. Ag-NOR, CMA 3 , DA/MM and NOR-FISH evidenced paracentromeric NORs on the short arm of 2nd pair chromosomes. Digestion with three restriction endonucleases followed by sequential staining with Giemsa, CMA 3 and DAPI evidenced on all chromosomes centromeric heterochromatin positive for both DAPI and CMA 3 . In situ hybridisation analysis showed the presence of an AT-rich satellite DNA in the centromeric heterochromatin of several chromosomes. A mosaicism was detected in the germinal cell lines of one specimen, as in six of the 20 plates examined the set had 37 chromosomes with a missing pair of telocentrics and an unpaired metacentric. Comparison of the chromosome sets of all the pectinids studied to date and comparison with a phyletic tree obtained from molecular mitochondrial genes studies yielded good agreement between karyotype morphology and taxonomic classification.

Research paper thumbnail of Characterization of Tc1 Transposon‐like sequences in Notothenioids

Http Dx Doi Org 10 1080 11250000009356366, Jan 28, 2009

Research paper thumbnail of Chromosomal Changes and Form-Formation, Subspeciation in the Wideranged Euroasian Species Zootoca vivipara (Evolution, Biogeography)

Russian Journal of Herpetology, May 29, 2013

Research paper thumbnail of Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf

Genetica, 1989

A detailed study on the genome of the lizard P. sicula has been carried out using restriction enz... more A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion ofgenomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCS1. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCS1 are present in other species of the same family of lizards.

Research paper thumbnail of Cryptic polyploidy in sharks and rays as relealed by DNA renaturation kinetics

Research paper thumbnail of DNA and chromosome evolution in lacertid lizards

Research paper thumbnail of Cytoflurometric DNA base determination in vertebrate species with different genome sizes

Basic and applied histochemistry

The base composition of DNA was studied in 15 amphibian species and 28 reptile species by means o... more The base composition of DNA was studied in 15 amphibian species and 28 reptile species by means of DAPI, a fluorochrome specific for adenine-thymine rich DNA (AT-rich DNA). The results obtained in reptiles and anuran amphibians coincided with biochemical data available for some species. In urodeles, on the contrary, the findings contrasted with biochemical data and suggest that DAPI is unable to stain all the AT-rich DNA in the erytrhocytes of these organisms.

Research paper thumbnail of Chromosome G-banding comparison among some Mediterranean lacertid lizards

Research paper thumbnail of On the heterochromatin of three Antarctic notothenioid fishes

Research paper thumbnail of The karyology of the Iberian rock lizards

Research paper thumbnail of Robertsonian fusions differentiate karyologically the populations of the Pyrenean rock lizards (Lacertidae, Archeolacerta)

Research paper thumbnail of Presenza di esemplari triploidi di rospo smeraldino (Bufo viridis Laurenti) in popolazioni perimediterranee

Research paper thumbnail of Novel LINE elements from the coelacanth Latimeria menadoensis

Research paper thumbnail of A novel satellite DNA isolated in Pecten jacobaeus shows high sequence similarity among molluscs

Molecular genetics and genomics : MGG, Jan 2, 2015

The aim of this work is to investigate the sequence conservation and the evolution of repeated DN... more The aim of this work is to investigate the sequence conservation and the evolution of repeated DNA in related species. Satellite DNA is a component of eukaryotic genomes and is made up of tandemly repeated sequences. These sequences are affected by high rates of mutation that lead to the occurrence of species-specific satellite DNAs, which are different in terms of both quantity and quality. In this work, a novel repetitive DNA family, named PjHhaI sat, is described in Pecten jacobaeus. The quantitative analyses revealed a different abundance of this element in the molluscan species investigated in agreement with the "library hypothesis" even if, in this case, at a high taxonomic level. In addition, the qualitative analysis demonstrated an astonishing sequence conservation not only among scallops but also in six other molluscan species belonging to three classes. These findings suggest that the PjHhaI sat may be considered as the most ancients of DNA described so far, whic...

Research paper thumbnail of Karyological and genetic variation in Middle Eastern lacertid lizards, Lacerta laevis and the Lacerta kulzeri complex: a case of chromosomal allopatric speciation

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2003

Karyological (standard and C, Ag-NOR and Alu-I banding methods) and mtDNA analyses (cytochrome b ... more Karyological (standard and C, Ag-NOR and Alu-I banding methods) and mtDNA analyses (cytochrome b and 12S rRNA) were conducted on specimens from eight allopatric populations of the Lacerta kulzeri complex. Parallel analyses were performed for comparison on Lacerta laevis specimens. Karyological and molecular studies support the morphological and ethological evidence indicating the specific separation between Lacerta laevis and Lacerta kulzeri. In the Lacerta kulzeri complex, chromosomal analysis substantiated an interpopulation differentiation roughly along a north-south trend, mainly regarding the sex chromosome morphology and heterochromatin. The cytochrome b and 12S rRNA gene analyses showed minor genetic differences that were considerably smaller than those commonly found in genetically isolated populations. The L. kulzeri populations from Barouk, Druze and Hermon show a mean genetic distance that, in other saurians, characterises subspecies. The conditions found in L. laevis and...

Research paper thumbnail of Ancient tetraploidy and slow molecular evolution in Scaphiophryne: ecological correlates of speciation mode in Malagasy relict amphibians

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2002

Karyotypes of three microhylid frog species of the Malagasy relict genus Scaphiophryne were studi... more Karyotypes of three microhylid frog species of the Malagasy relict genus Scaphiophryne were studied: Scaphiophryne gottlebei, S. madagascariensis and S. spinosa. The latter two showed a plesiomorphic ranoid karyotype of 2n = 26. In contrast, tetraploidy was demonstrated in S. gottlebei, which constitutes an exceptional state among Malagasy amphibians. A combination of different banding techniques and of rDNA-FISH provided evidence for allopolyploidy in the species and for a completed subsequent functional and structural diploidization. Phylogenetic analysis of mitochondrial 16S rDNA sequences revealed a significant deceleration of nucleotide substitution rates in Scaphiophryne. The tetraploidy of S. gottlebei probably occurred early in their radiation. Ecological and behavioural patterns of Scaphiophryne probably favoured intraspecific gene flow and hybridization events, thereby leading to slow molecular substitution rates and to allopolyploid chromosome speciation in S. gottlebei.

Research paper thumbnail of Genome size evolution in vertebrates: Trends and constraints

Comparative Biochemistry and Physiology Part B Comparative Biochemistry, Feb 1, 1989

Research paper thumbnail of New Data on Karyotype of Some Scincidae

Caryologia, 1987

The karyotypes of two scincid species (Eumeces inexpectatus and Sphenops sphenopsi/ormis) have be... more The karyotypes of two scincid species (Eumeces inexpectatus and Sphenops sphenopsi/ormis) have been studied. E. inexpectatus shows 12 biarmed macrochromosomes and 14 microchromosomes. This karyotype is similar to that observed in some species of the same genus. S. sphenopsi/ormis shows, instead, 16 biarmed and 4 uniarmed macrochromosomes and 8 microchromosomes. This karyotype is very similar to that possessed by the Chalcides species studied so far. It is suggested that S. sphenopsiformis is closer to the genus Chalcides than what has been supposed previously. It is also supposed that the karyotypes of S. sphenopsiformis and C. chalcides might be derived, through a series of pericentric inversions, from that of C. ocellatus tiligugu, in which all the macrochromosomes are biarmed.

Research paper thumbnail of The karyology of Mediterranean lacertid lizards

Research paper thumbnail of Results and Perspectives in the Study of Scincomorph Karyology

Research paper thumbnail of Karyology of the Antarctic scallop Adamussium colbecki , with some comments on the karyological evolution of pectinids

Genetica, Jun 1, 2006

Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and comp... more Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n=38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two telocentrics. Ag-NOR, CMA 3 , DA/MM and NOR-FISH evidenced paracentromeric NORs on the short arm of 2nd pair chromosomes. Digestion with three restriction endonucleases followed by sequential staining with Giemsa, CMA 3 and DAPI evidenced on all chromosomes centromeric heterochromatin positive for both DAPI and CMA 3 . In situ hybridisation analysis showed the presence of an AT-rich satellite DNA in the centromeric heterochromatin of several chromosomes. A mosaicism was detected in the germinal cell lines of one specimen, as in six of the 20 plates examined the set had 37 chromosomes with a missing pair of telocentrics and an unpaired metacentric. Comparison of the chromosome sets of all the pectinids studied to date and comparison with a phyletic tree obtained from molecular mitochondrial genes studies yielded good agreement between karyotype morphology and taxonomic classification.

Research paper thumbnail of Characterization of Tc1 Transposon‐like sequences in Notothenioids

Http Dx Doi Org 10 1080 11250000009356366, Jan 28, 2009

Research paper thumbnail of Chromosomal Changes and Form-Formation, Subspeciation in the Wideranged Euroasian Species Zootoca vivipara (Evolution, Biogeography)

Russian Journal of Herpetology, May 29, 2013

Research paper thumbnail of Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf

Genetica, 1989

A detailed study on the genome of the lizard P. sicula has been carried out using restriction enz... more A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion ofgenomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCS1. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCS1 are present in other species of the same family of lizards.

Research paper thumbnail of Cryptic polyploidy in sharks and rays as relealed by DNA renaturation kinetics

Research paper thumbnail of DNA and chromosome evolution in lacertid lizards

Research paper thumbnail of Cytoflurometric DNA base determination in vertebrate species with different genome sizes

Basic and applied histochemistry

The base composition of DNA was studied in 15 amphibian species and 28 reptile species by means o... more The base composition of DNA was studied in 15 amphibian species and 28 reptile species by means of DAPI, a fluorochrome specific for adenine-thymine rich DNA (AT-rich DNA). The results obtained in reptiles and anuran amphibians coincided with biochemical data available for some species. In urodeles, on the contrary, the findings contrasted with biochemical data and suggest that DAPI is unable to stain all the AT-rich DNA in the erytrhocytes of these organisms.

Research paper thumbnail of Chromosome G-banding comparison among some Mediterranean lacertid lizards

Research paper thumbnail of On the heterochromatin of three Antarctic notothenioid fishes

Research paper thumbnail of The karyology of the Iberian rock lizards

Research paper thumbnail of Robertsonian fusions differentiate karyologically the populations of the Pyrenean rock lizards (Lacertidae, Archeolacerta)

Research paper thumbnail of Presenza di esemplari triploidi di rospo smeraldino (Bufo viridis Laurenti) in popolazioni perimediterranee

Research paper thumbnail of Novel LINE elements from the coelacanth Latimeria menadoensis

Research paper thumbnail of A novel satellite DNA isolated in Pecten jacobaeus shows high sequence similarity among molluscs

Molecular genetics and genomics : MGG, Jan 2, 2015

The aim of this work is to investigate the sequence conservation and the evolution of repeated DN... more The aim of this work is to investigate the sequence conservation and the evolution of repeated DNA in related species. Satellite DNA is a component of eukaryotic genomes and is made up of tandemly repeated sequences. These sequences are affected by high rates of mutation that lead to the occurrence of species-specific satellite DNAs, which are different in terms of both quantity and quality. In this work, a novel repetitive DNA family, named PjHhaI sat, is described in Pecten jacobaeus. The quantitative analyses revealed a different abundance of this element in the molluscan species investigated in agreement with the "library hypothesis" even if, in this case, at a high taxonomic level. In addition, the qualitative analysis demonstrated an astonishing sequence conservation not only among scallops but also in six other molluscan species belonging to three classes. These findings suggest that the PjHhaI sat may be considered as the most ancients of DNA described so far, whic...

Research paper thumbnail of Karyological and genetic variation in Middle Eastern lacertid lizards, Lacerta laevis and the Lacerta kulzeri complex: a case of chromosomal allopatric speciation

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2003

Karyological (standard and C, Ag-NOR and Alu-I banding methods) and mtDNA analyses (cytochrome b ... more Karyological (standard and C, Ag-NOR and Alu-I banding methods) and mtDNA analyses (cytochrome b and 12S rRNA) were conducted on specimens from eight allopatric populations of the Lacerta kulzeri complex. Parallel analyses were performed for comparison on Lacerta laevis specimens. Karyological and molecular studies support the morphological and ethological evidence indicating the specific separation between Lacerta laevis and Lacerta kulzeri. In the Lacerta kulzeri complex, chromosomal analysis substantiated an interpopulation differentiation roughly along a north-south trend, mainly regarding the sex chromosome morphology and heterochromatin. The cytochrome b and 12S rRNA gene analyses showed minor genetic differences that were considerably smaller than those commonly found in genetically isolated populations. The L. kulzeri populations from Barouk, Druze and Hermon show a mean genetic distance that, in other saurians, characterises subspecies. The conditions found in L. laevis and...

Research paper thumbnail of Ancient tetraploidy and slow molecular evolution in Scaphiophryne: ecological correlates of speciation mode in Malagasy relict amphibians

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2002

Karyotypes of three microhylid frog species of the Malagasy relict genus Scaphiophryne were studi... more Karyotypes of three microhylid frog species of the Malagasy relict genus Scaphiophryne were studied: Scaphiophryne gottlebei, S. madagascariensis and S. spinosa. The latter two showed a plesiomorphic ranoid karyotype of 2n = 26. In contrast, tetraploidy was demonstrated in S. gottlebei, which constitutes an exceptional state among Malagasy amphibians. A combination of different banding techniques and of rDNA-FISH provided evidence for allopolyploidy in the species and for a completed subsequent functional and structural diploidization. Phylogenetic analysis of mitochondrial 16S rDNA sequences revealed a significant deceleration of nucleotide substitution rates in Scaphiophryne. The tetraploidy of S. gottlebei probably occurred early in their radiation. Ecological and behavioural patterns of Scaphiophryne probably favoured intraspecific gene flow and hybridization events, thereby leading to slow molecular substitution rates and to allopolyploid chromosome speciation in S. gottlebei.