Surekha K Satpute | Savitribai Phule Pune University (original) (raw)

Papers by Surekha K Satpute

Different screening methods namely, hemolytic assay (HA), modified drop collapse (MDC), tilted gl... more Different screening methods namely, hemolytic assay (HA), modified drop collapse (MDC), tilted glass slide (TGST), oil spread method (OSM), blue agar plate (BAP), hydrocarbon overlaid agar (HOA) plate, emulsification index (EI), emulsification assay (EA) were assessed for their efficiency to detect biosurfactant producing marine bacteria. Forty-five strains of bacteria, comprising 18 Acinetobacter and 27 other bacteria along with positive MTCC reference strains were examined. HA, MDC, TGST efficiently detected 15, 17 and 14 biosurfactant producers respectively. Five hemolytic cultures did not show any biosurfactant production in MDC, TGST, and/or OSM. The emulsification of kerosene was also poorer. These results suggest that HA is not totally reliable. Six bacterial isolates produced biosurfactant in OSM, and MDC as well as TGST. MDC and TGS tests demonstrated good activity for nine isolates and proved to be the essential methods. None of the bacteria produced glycolipid on BAP. Cul...

Biological surfactants particularly of microbial origin have recently been gaining increased inte... more Biological surfactants particularly of microbial origin have recently been gaining increased interest in the surfactants markets. Diverse functional properties viz., surface, interfacial tension, foaming detergency and wettability of biosurfactant (BS) / bioemulsifier (BE) facilitate their wide application potentials in the industrial sector. Petrochemical based surfactant / emulsifiers do exhibit similar properties however, their toxic and non-biodegradable characteristics limit their uses or application in many industries particularly food related. The opportunities offered by BS/BE are encouraging for commercial exploitation particularly due to other beneficial properties to food industries such as antimicrobial, antibiofilm and antiadhesive, non-fouling utilities. Safety and freshness are essential for ingredients/components used in food/feed industries and BS properties mentioned above makes them highly applicable to such industries. This chapter deals with role of BS/BE in var...

One of the necessities of a healthy life is to have adequate amounts of antioxidants in the body ... more One of the necessities of a healthy life is to have adequate amounts of antioxidants in the body and neutralize the damage caused by free radicals. Free radicals are continuously produced in the human body, as they are essential for energy supply, detoxification, chemical signaling and immune function but their over-production due to exposure to external oxidant substances or a failure in the defense mechanisms, increases risk of different disease. Powerful antioxidants perform multiple functions essential for good health and help organisms deal with oxidative stress, caused by free radical damage. Natural production of antioxidants in body decreases with time and age, so it becomes essential to include antioxidant rich food items in the diet. In the recent years, in the attempt to counteract the detrimental effects of oxidative damages is always more convincing the strategy of implementing the diet with antioxidants nutrients, especially deriving from natural sources. Literature s...

Brazilian Journal of Microbiology

RSC Advances, 2017

The synthesis and characterization of 2-((pyridine-2-yl)methylamino)naphthalene-1,4-dione (H-1), ... more The synthesis and characterization of 2-((pyridine-2-yl)methylamino)naphthalene-1,4-dione (H-1), 2-((thiophen-2-yl)methylamino)naphthalene-1,4-dione (H-2) and 2-((pyridine/thiophen-2-yl)ethylamino) naphthalene-1,4-dione (H-3 and H-4) have been carried out. Molecular recognition abilities of these ligands toward transition metal ions in methanol, methanol-water, methanol-triethylamine or methanol-watertriethylamine mixtures, stoichiometries and association constants of H-1 and H-3 have been determined. It has been shown that H-1 and H-3 coordinate to metal ions via two nitrogen atoms and oxygen and exhibit remarkable selectivity towards Cu 2+ ions in methanol or methanol-water mixtures, the complexation being accompanied by a color change from orange to intense blue. LOD (Limit of Detection) of Cu 2+ with H-1, H-3 are 1.48 Â 10 À8 mol L À1 and 1.59 Â 10 À8 mol L À1 respectively. The vibrational spectra, 1 H NMR chemical shifts and optical properties of H-1 to H-4 derived from density functional theory are also presented.

Coatings

In the current era, an ever-emerging threat of multidrug-resistant (MDR) pathogens pose serious h... more In the current era, an ever-emerging threat of multidrug-resistant (MDR) pathogens pose serious health challenges to mankind. Researchers are uninterruptedly putting their efforts to design and develop alternative, innovative strategies to tackle the antibiotic resistance displayed by varied pathogens. Among several naturally derived and chemically synthesized compounds, quinones have achieved a distinct position to defeat microbial pathogens. This review unleashes the structural diversity and promising biological activities of naphthoquinones (NQs) and their derivatives documented in the past two decades. Further, realizing their functional potentialities, researchers were encouraged to approach NQs as lead molecules. We have retrieved information that is dedicated on biological applications (antibacterial, antifungal, antiparasitic) of NQs. The multiple roles of NQs offer them a promising armory to combat microbial pathogens including MDR and the ESKAPE (Enterococcus faecium, Stap...

Frontiers in Bioengineering and Biotechnology

Biocatalysis and Agricultural Biotechnology

New Journal of Chemistry

Design and development of drugs in multi-drug resistant (MDR) infections have been of growing int... more Design and development of drugs in multi-drug resistant (MDR) infections have been of growing interest. We report syntheses, antibacterial and antifungal activities of imidazole-based 1,4-naphthoquinones (I-1 to I-4; 1-alkyl-2-methyl-1H-naphtho[2,3-d]imidazole-4,9-dione(alkyl =...

Frontiers in Microbiology

Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which fac... more Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current research deals with isolation of Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water for BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach to explore the genomic insight of the strain SAMP and displayed the presence of gene for BS biosynthesis. The genome analysis revealed this potential to be intrinsic to the strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) and oil displacement (OD) showed SAMP MCC 3013 as a potent BS producer. BS reduced SFT of phosphate buffer saline (PBS) pH: 7.0 from 72 to 30 mN/m with a critical micelle concentration (CMC) value of 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (1 H NMR and 13 C NMR) and liquid chromatography mass spectrometry (LC-MS) revealed terpene containing BS having sugar, lipid moieties. The genomic sequence analysis of P. maritimus SAMP showed complete genes in the pathway for the synthesis of terpenoid. Probably terpenoid is the accountable backbone molecule for the BS production, but the later stages of terpenoid conversion to the BS could not be found. Moreover, it is important to highlight that till today; no single report documents the in-detailed physico-chemical characterization of BS from Planococcus sp. Based on genomic and functional properties, the term terpene containing BS is denoted for the surfactant produced by P. maritimus.

International Journal of Environmental Health Research

Journal of Molecular Structure

BMC Microbiology

Background: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the ... more Background: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903. Results: Extracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6 mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane-PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains. Conclusion: The current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices.

Environmental Sustainability

Biosurfactants (BSs) are amphiphilic compounds produced by microorganisms and have diverse applic... more Biosurfactants (BSs) are amphiphilic compounds produced by microorganisms and have diverse applications in various industries. This study was aimed to identify and characterize BS producing bacteria from three oil-polluted sites from Pune, Maharashtra, India. Out of the total 48 bacterial isolates, 14 BS producing isolates were detected through drop collapse method (DCM) and oil spreading method (OSM), hemolytic activity, cetyl trimethyl ammonium bromide (CTAB) test, emulsification index (E 24), and surface tension (SFT) measurement. The BS producing isolates were identified by matrix-assisted laser desorption/ionization time of flight mass spectrophotometry (MALDI-TOF MS) and 16S rRNA gene sequencing. Results indicated that the isolates belonged to six genera, including Bacillus, Agrococcus, Arthrobacter, Kocuria, Dietzia, and Lysinibacillus. We are reporting BS producing Agrococcus terreus for the first time. Out of 14 BS producing bacteria, four isolates showed β hemolysis in blood agar plates, while all isolates showed negative results on CTAB agar plate. A positive correlation was observed between DCM, OSM, and SFT measurements. Bacillus sp. (isolate PR170) presented the maximum diameter of the clear zone (32 mm) in OSM. The E 24 was found to be in the range of 15-57%. Four isolates including PR170, PP151, PP168, and PG138 significantly reduced the SFT of the medium by up to 26.79, 27.81, 28.83, and 30.99 mN/m, respectively. Nine isolates were able to grow in media containing various polyaromatic hydrocarbons. Results gathered in this study demonstrated that isolates belonging to four genera from the contaminated sites had an outstanding potential for further application in biotechnological processes such as large-scale BS production or bioremediation of hydrocarbon polluted sites, and will contribute towards environmental sustainability.

Applied Sciences

Background: Biomedical devices and implants are adversely affected by biofilm-associated infectio... more Background: Biomedical devices and implants are adversely affected by biofilm-associated infections that pose serious public health issues. Biosurfactants (BSs) can combat pathogenic biofilms through their antimicrobial, antibiofilm and antiadhesive capabilities. The objective of our research was to produce biosurfactant (BS) from Lactobacillus acidophilus NCIM 2903 and investigate its antibiofilm, antiadhesive potential using microfluidics strategies by mimicking the micro-environment of biofilm. Methods: Antibiofilm and antiadhesive potential was effectively evaluated using different methods like microfluidics assay, catheter assay, polydimethlysiloxane (PDMS) disc assay. Along with this chemical and physical characteristics of BS were also evaluated. Results: Cell free biosurfactant (CFBS) obtained was found to be effective against biofilm which was validated through the microfluidic (MF) or Lab on Chip (LOC) approach. The potency of CFBS was also evaluated on catheter tubing and...

Management Systems in Production Engineering, 2017

A strong developed bio-based industrial sector will significantly reduce dependency on fossil res... more A strong developed bio-based industrial sector will significantly reduce dependency on fossil resources, help the countries meet climate change targets, and lead to greener and more environmental friendly growth. The key is to develop new technologies to sustainably transform renewable natural resources into bio-based products and biofuels. Biomass is a valuable resource and many parameters need to be taken in to account when assessing its use and the products made from its. The bioeconomy encompass the production of renewable biological resources and their conversion into food, feed and bio-based products (chemicals, materials and fuels) via innovative and efficient technologies provided by industrial biotechnology. The paper presents the smart and efficient way to use the agro-industrial, dairy and food processing wastes for biosurfactant’s production. Clarification processes are mandatory to use the raw substrates for microbial growth as well as biosurfactant production for comme...

Applied Microbiology and Biotechnology, 2016

The perpetual use of antibiotics against pathogens inadvertently altered their genes that have tr... more The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic inorganic nanoparticle-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than organic-based antibiotics. Based on this line, herein, we present observations on microbial abatement using gold-based zinc oxide nanostructures (Au@ZnO) which are synthesized using hydrothermal route. Inhibition of microbial growth and biofilm using Au@ZnO is a unique feature of our study. Furthermore, this study evinces antimicrobial and antibiofilm mechanisms of photo-eradiated Au@ZnO by disruption of cellular functions and biofilms via reactive oxygen species (ROS)-dependent generation of superoxide anion radical. The present study is significant as it introduces novel functionalities to Au@ZnO in the biomedical field which can be extended to other species of microbial pathogens.