Dhanaji M Ghadage | Shivaji University, Kolhapur, India (original) (raw)

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Papers by Dhanaji M Ghadage

Research paper thumbnail of Micropropagation, molecular profiling and RP-HPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.)

Industrial Crops and Products, 2015

ABSTRACT

Research paper thumbnail of Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Journal of Liquid Chromatography & Related Technologies, 2015

ABSTRACT Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabeti... more ABSTRACT Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

Research paper thumbnail of Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Biochemistry and Biophysics Reports

The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals... more The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography-diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1-diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications.

Research paper thumbnail of Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and ant... more Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using
continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted
extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse
phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational
parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted
higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed
by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a
solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the
RP-HPLC technique for identification and quantification.

Research paper thumbnail of Micropropagation, molecular profiling and RP-HPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.)

Salacia chinensis L. (family – Hippocrateaceae) is a convenient source for industrial scale isola... more Salacia chinensis L. (family – Hippocrateaceae) is a convenient source for industrial scale isolation of antidiabetic and anticancerous drug ‘mangiferin’ which is extensively used in both traditional and modern medicines. High demands from pharmaceutical industries lead the indiscriminate harvesting from the wild which created the pressure on natural populations. Micropropagation system was developed using different explants for both commercialization and conservation of S. chinensis. Murashige and Skoog’s (MS) medium with different concentrations and combinations of plant growth regulators (PGRs) viz. 6-benzylaminopurine (BAP), kinetin (KN), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), -naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid (2,4-D) were used during various in vitro regeneration stages. Maximum rate of shoot multiplication (75%) and maximum number of shoots(6.7 ± 1.0) were obtained with MS medium fortified with BAP (2.0 mg/l), NAA (0.8 mg/l) and ascorbic acid(100 mg/l). The antioxidants and other additives significantly altered the rate of shoot multiplication,number of shoots as well as survival rate of shoots. The plantlets rooted on ½MS + IBA 1.5 mg/l, showed 80% establishment in soil without any morphological variation. The genetic integrity of the regenerated plants was further confirmed using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analysis. A total of 3871 distinct bands were produced by 12 RAPD and 17 ISSR primers in mother and 20 randomly selected micropropagated clones wherein 3858 (99.66%) bands were monomorphic and rest of 13 (0.34%) were polymorphic. The high level of monomorphism confirms the genetic uniformity of the micropropagated clones. Additionally, reverse phase-high performance liquid chromatographic (RP-HPLC) analysis at various in vitro regeneration stages showed uniformity in terms of presence of mangiferin however, the amount of mangiferin varied across regeneration stages. Phytochemical and molecular fingerprinting of micropropagated clones revealed that the in vitro regeneration protocol developed could be successfully used for industrial-scale propagation of S. chinensis with enhanced production of mangiferin.

Research paper thumbnail of Micropropagation, molecular profiling and RP-HPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.)

Industrial Crops and Products, 2015

ABSTRACT

Research paper thumbnail of Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Journal of Liquid Chromatography & Related Technologies, 2015

ABSTRACT Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabeti... more ABSTRACT Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

Research paper thumbnail of Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Biochemistry and Biophysics Reports

The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals... more The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography-diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1-diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications.

Research paper thumbnail of Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and ant... more Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using
continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted
extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse
phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational
parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted
higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed
by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a
solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the
RP-HPLC technique for identification and quantification.

Research paper thumbnail of Micropropagation, molecular profiling and RP-HPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.)

Salacia chinensis L. (family – Hippocrateaceae) is a convenient source for industrial scale isola... more Salacia chinensis L. (family – Hippocrateaceae) is a convenient source for industrial scale isolation of antidiabetic and anticancerous drug ‘mangiferin’ which is extensively used in both traditional and modern medicines. High demands from pharmaceutical industries lead the indiscriminate harvesting from the wild which created the pressure on natural populations. Micropropagation system was developed using different explants for both commercialization and conservation of S. chinensis. Murashige and Skoog’s (MS) medium with different concentrations and combinations of plant growth regulators (PGRs) viz. 6-benzylaminopurine (BAP), kinetin (KN), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), -naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid (2,4-D) were used during various in vitro regeneration stages. Maximum rate of shoot multiplication (75%) and maximum number of shoots(6.7 ± 1.0) were obtained with MS medium fortified with BAP (2.0 mg/l), NAA (0.8 mg/l) and ascorbic acid(100 mg/l). The antioxidants and other additives significantly altered the rate of shoot multiplication,number of shoots as well as survival rate of shoots. The plantlets rooted on ½MS + IBA 1.5 mg/l, showed 80% establishment in soil without any morphological variation. The genetic integrity of the regenerated plants was further confirmed using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analysis. A total of 3871 distinct bands were produced by 12 RAPD and 17 ISSR primers in mother and 20 randomly selected micropropagated clones wherein 3858 (99.66%) bands were monomorphic and rest of 13 (0.34%) were polymorphic. The high level of monomorphism confirms the genetic uniformity of the micropropagated clones. Additionally, reverse phase-high performance liquid chromatographic (RP-HPLC) analysis at various in vitro regeneration stages showed uniformity in terms of presence of mangiferin however, the amount of mangiferin varied across regeneration stages. Phytochemical and molecular fingerprinting of micropropagated clones revealed that the in vitro regeneration protocol developed could be successfully used for industrial-scale propagation of S. chinensis with enhanced production of mangiferin.