Pasquale Bandiera | Università di Sassari (original) (raw)
Papers by Pasquale Bandiera
Italian journal of anatomy and embryology, 2014
In the early months of 2011, during the restoration of Sant’Antonio Abate’s Cathedral in Castelsa... more In the early months of 2011, during the restoration of Sant’Antonio Abate’s Cathedral in Castelsardo came to light environments remained hidden so far, which aroused considerable interest both for the archaeological and anthropological point of view for the discovery of a cemetery crypt with numerous human remains dating from the eighteenth to nineteenth century. Archaeological investigations took place at different times and in different ways during the months of January to April of 2011, which allowed to detect a burial site, with a great number of individuals organized in several layers piled up at the bottom of the crypt, and to define some stages in the building of the church, mainly in its monumental transition from Romanesque structure to new cathedral seat of the diocese. Eighteen individuals of both sexes, were finally identified at different levels, including 16 partially mummified and 2 almost entirelly mummified deposited on a sort of open plank, that, from the archeolog...
Italian journal of anatomy and embryology, 2015
Multiple Sclerosis (MS) is the most common chronic disease of central nervous system (CNS). MS pa... more Multiple Sclerosis (MS) is the most common chronic disease of central nervous system (CNS). MS patients may present with a wide variety of neurologic symptoms, including fatigue, sensory and motor disturbances, depression, cognitive disorders, bladder, bowel and sexual dysfunctions (1). Pelvic floor disorders are often overlooked symptoms in patients with MS (2). With respect to the general population, constipation and/or faecal incontinence are more frequent in MS, with an estimated prevalence ranging from 52% to 68% (3). The neuro-pathophysiological mechanism of bowel dysfunction in MS remains unclear. Several factors act in concert to maintain fecal continence; among the anatomical factors the anal sphincter is crucial, interplaying with rectum and pelvic floor muscles to controll defecation. In recent decades, technological advances in diagnostic imaging have dramatically improved our knowledge favouring the assessment of anatomical and functional deficits in patients with bowel...
Italian journal of anatomy and embryology, 2002
There is a wide range of research projects carried out within our Department of Biomedical Scienc... more There is a wide range of research projects carried out within our Department of Biomedical Sciences about the environmental consequences caused by toxic heavy metals: we have demonstrated by in vitro experiments that cadmium can induce ultrastructural alterations, with different degree, in the structure of human fetal liver. The goal of this study was to develop our research testing in vivo in experimental animal model the results obtained.
Because of its high rates of soil-to-planttransfer, Cd is a contaminant found in most human foods... more Because of its high rates of soil-to-planttransfer, Cd is a contaminant found in most human foodstuffs, which renders diet a primary source of exposure among nonsmoking, non-occupationally exposed populations. A safe intake limit of 7 μg cadmium/week/kg body weight was set based on the critical renal cadmium concentration of between 100 and 200 μg/g wet weight. The aim of our research was to study Cd effects on morphology of the glomerular and tubular cells of kidney in experimental animal.
Italian journal of anatomy and embryology, 2015
The paleonutrition study of ancient people mainly affects the teeth as the outer and the stronges... more The paleonutrition study of ancient people mainly affects the teeth as the outer and the strongest part of the digestive tract, perfused with blood and lymphatic vessels; bring a lot of information that may be useful to understand the nourishment in the past. The aim of this study is to identify possible correlations between the thickness of the enamel and the presence of trace elements as indicators of Palaeolithic diet. The samples came from seven different Sardinian sites dated back to the 3000 bC and 1400 AD. previously subjected to a classical anthropological study, were washed in absolute acetone and rinsed in distilled water. To calculate the amount of enamel on the teeth and its correlation with the dentin, we chose to analyse the samples with micro-CT oriented perpendicular to the occlusal plane with 360° rotation. For the qualitative estimation of trace elements, we used a X-ray fluorescence spectrometer. The study on the thickness of the enamel showed that the total area ...
Italian journal of anatomy and embryology, 2016
Metals have strong toxic effects in humans and can act as immunoregulatory factors. The purpose o... more Metals have strong toxic effects in humans and can act as immunoregulatory factors. The purpose of our study was to determine whether the concentrations of metals are associated with the clinical course of nasal polyposis (NP). We measured the concentrations of 10 metals (Zn, Mn, Se, Fe, Cr, Ni, Pb, Al, Cd, and Cu) in 58 patients with NP, and 29 controls with a healthy nasal mucosa. We used electron microscopy to compare the ultrastructural features of the nasal mucosa between NP patients and healthy controls. Concentrations of metals in nasal polyps and healthy mucosa were determined by mass spectrometry. Transmission electron microscopic (TEM) and scanning electron microscopic (SEM) images of the nasal mucosa were obtained. The mean tissue concentrations of all 10 metals were significantly lower in NP patients than in healthy controls (P < 0.001). Tissue concentrations of each metal were lower in stages III and IV NP than in stages I and II NP, although the differences were not...
Cells, 2020
Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging ... more Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging and skin related damages. In addition, recently achieved progress in nanomedicine opens a novel approach yielding from combination of modern nanotechnology with traditional treatment for innovative pharmacotherapeutics. In the present study, we investigated the antiaging effect of a pretreatment with Myrtus communis natural extract combined with a polycaprolactone nanofibrous scaffold (NanoPCL-M) on skin cell populations exposed to UV. We set up a novel model of skin on a bioreactor mimicking a crosstalk between keratinocytes, stem cells and fibroblasts, as in skin. Beta-galactosidase assay, indicating the amount of senescent cells, and viability assay, revealed that fibroblasts and stem cells pretreated with NanoPCL-M and then exposed to UV are superimposable to control cells, untreated and unexposed to UV damage. On the other hand, cells only exposed to UV stress, without NanoPCL-M pre...
Cells, 2021
Tissue homeostasis mainly depends on the activity of stem cells to replace damaged elements and r... more Tissue homeostasis mainly depends on the activity of stem cells to replace damaged elements and restore tissue functions. Within this context, mesenchymal stem cells and fibroblasts are essential for maintaining tissue homeostasis in skin, in particular in the dermis. Modifications in collagen fibers are able to affect stem cell features. Skin properties can be significantly reduced after injuries or with aging, and stem cell niches, mainly comprising extracellular matrix (ECM), may be compromised. To this end, specific molecules can be administrated to prevent the aging process induced by UV exposure in the attempt to maintain a youngness phenotype. NanoPCL-M is a novel nanodevice able to control delivery of Mediterranean plant myrtle (Myrtus communis L.) extracts. In particular, we previously described that myrtle extracts, rich in bioactive molecules and nutraceuticals, were able to counteract senescence in adipose derived stem cells. In this study, we analyzed the effect of Nano...
Nature Communications, 2020
The island of Sardinia has been of particular interest to geneticists for decades. The current mo... more The island of Sardinia has been of particular interest to geneticists for decades. The current model for Sardinia’s genetic history describes the island as harboring a founder population that was established largely from the Neolithic peoples of southern Europe and remained isolated from later Bronze Age expansions on the mainland. To evaluate this model, we generate genome-wide ancient DNA data for 70 individuals from 21 Sardinian archaeological sites spanning the Middle Neolithic through the Medieval period. The earliest individuals show a strong affinity to western Mediterranean Neolithic populations, followed by an extended period of genetic continuity on the island through the Nuragic period (second millennium BCE). Beginning with individuals from Phoenician/Punic sites (first millennium BCE), we observe spatially-varying signals of admixture with sources principally from the eastern and northern Mediterranean. Overall, our analysis sheds light on the genetic history of Sardini...
Radiology Case Reports, 2019
The craniovertebral junction is a unique part of the somite-derived axial skeleton. The absence o... more The craniovertebral junction is a unique part of the somite-derived axial skeleton. The absence or hypoplasia of the posterior arch of C1 is frequently associated with compensatory hypertrophy of the anterior arch of C1 and of the spinous process of C2. Here, we report a patient with agenesis of the posterior arch of C1 without neurologic deficits. Our patient presented with complex alterations of the craniovertebral junction that involved interactions between the condyles, clivus, atlas, and epistropheus. To our knowledge, dislocation of the odontoid process above the Chamberlain line, including cranial migration of the anterior arch of C1, has not been reported in the literature.
Radiology Case Reports, 2019
Klippel-Feil syndrome is a congenital malformation characterized by the fusion of at least 2 cerv... more Klippel-Feil syndrome is a congenital malformation characterized by the fusion of at least 2 cervical vertebrae. It may occur in association with other clinical syndromes and disorders. We describe a case of prenatal diagnosis of a Klippel-Feil syndrome with Dandy-Walker malformation, and spina bifida, proved by ultrasound examination. A postmortem x-ray and autopsy were performed in a female fetus of 16 + 6 weeks of gestation: several malformations have been discovered. To the best of our knowledge, no similar cases have been reported in the medical literature. This case report underscores the importance of a careful ultrasound screening during pregnancy for an adequate diagnostic and therapeutic management.
Anatomical Sciences Education, 2019
the Achille's heel of medical students. A systematic analysis of educational strategies for the t... more the Achille's heel of medical students. A systematic analysis of educational strategies for the teaching of neuroanatomy. Anatomical Sciences Education 13 (1) , pp.
Advances in Infectious Diseases, 2017
Malaria has been an important disease throughout the history, particularly in the Mediterranean. ... more Malaria has been an important disease throughout the history, particularly in the Mediterranean. The island of Sardinia (Italy) was characterized by severe endemic malaria from antiquity until its eradication in 1950. It provides an exceptional and unique site to examine the history of the disease and its effects on human biology and ecology. Endemic malaria can cause chronic hemolytic anemias that may result in osteological signatures. This paper examines these markers, Porotic Hyperostosis (PH) and Cribra Orbitalia (CO), which are identified from skeletons uncovered in archaeological excavations in Northern Sardinia. Previous hypotheses based on historical references and genetic data suggest that the disease was absent on the island in prehistory and was imported, probably by slaves, during the Carthaginian period (6th to 3rd century BC). This article reports on the paleontological analysis of 283 skeletons excavated from seven archeological sites dating from 4700 BP to 1582 AD. Osteological remains were examined for markers CO and PH as a proxy variable for malaria. The findings support previous hypotheses about history of malaria over time.
Italian journal of anatomy and embryology, 2014
In the early months of 2011, during the restoration of Sant’Antonio Abate’s Cathedral in Castelsa... more In the early months of 2011, during the restoration of Sant’Antonio Abate’s Cathedral in Castelsardo came to light environments remained hidden so far, which aroused considerable interest both for the archaeological and anthropological point of view for the discovery of a cemetery crypt with numerous human remains dating from the eighteenth to nineteenth century. Archaeological investigations took place at different times and in different ways during the months of January to April of 2011, which allowed to detect a burial site, with a great number of individuals organized in several layers piled up at the bottom of the crypt, and to define some stages in the building of the church, mainly in its monumental transition from Romanesque structure to new cathedral seat of the diocese. Eighteen individuals of both sexes, were finally identified at different levels, including 16 partially mummified and 2 almost entirelly mummified deposited on a sort of open plank, that, from the archeolog...
Italian journal of anatomy and embryology, 2015
Multiple Sclerosis (MS) is the most common chronic disease of central nervous system (CNS). MS pa... more Multiple Sclerosis (MS) is the most common chronic disease of central nervous system (CNS). MS patients may present with a wide variety of neurologic symptoms, including fatigue, sensory and motor disturbances, depression, cognitive disorders, bladder, bowel and sexual dysfunctions (1). Pelvic floor disorders are often overlooked symptoms in patients with MS (2). With respect to the general population, constipation and/or faecal incontinence are more frequent in MS, with an estimated prevalence ranging from 52% to 68% (3). The neuro-pathophysiological mechanism of bowel dysfunction in MS remains unclear. Several factors act in concert to maintain fecal continence; among the anatomical factors the anal sphincter is crucial, interplaying with rectum and pelvic floor muscles to controll defecation. In recent decades, technological advances in diagnostic imaging have dramatically improved our knowledge favouring the assessment of anatomical and functional deficits in patients with bowel...
Italian journal of anatomy and embryology, 2002
There is a wide range of research projects carried out within our Department of Biomedical Scienc... more There is a wide range of research projects carried out within our Department of Biomedical Sciences about the environmental consequences caused by toxic heavy metals: we have demonstrated by in vitro experiments that cadmium can induce ultrastructural alterations, with different degree, in the structure of human fetal liver. The goal of this study was to develop our research testing in vivo in experimental animal model the results obtained.
Because of its high rates of soil-to-planttransfer, Cd is a contaminant found in most human foods... more Because of its high rates of soil-to-planttransfer, Cd is a contaminant found in most human foodstuffs, which renders diet a primary source of exposure among nonsmoking, non-occupationally exposed populations. A safe intake limit of 7 μg cadmium/week/kg body weight was set based on the critical renal cadmium concentration of between 100 and 200 μg/g wet weight. The aim of our research was to study Cd effects on morphology of the glomerular and tubular cells of kidney in experimental animal.
Italian journal of anatomy and embryology, 2015
The paleonutrition study of ancient people mainly affects the teeth as the outer and the stronges... more The paleonutrition study of ancient people mainly affects the teeth as the outer and the strongest part of the digestive tract, perfused with blood and lymphatic vessels; bring a lot of information that may be useful to understand the nourishment in the past. The aim of this study is to identify possible correlations between the thickness of the enamel and the presence of trace elements as indicators of Palaeolithic diet. The samples came from seven different Sardinian sites dated back to the 3000 bC and 1400 AD. previously subjected to a classical anthropological study, were washed in absolute acetone and rinsed in distilled water. To calculate the amount of enamel on the teeth and its correlation with the dentin, we chose to analyse the samples with micro-CT oriented perpendicular to the occlusal plane with 360° rotation. For the qualitative estimation of trace elements, we used a X-ray fluorescence spectrometer. The study on the thickness of the enamel showed that the total area ...
Italian journal of anatomy and embryology, 2016
Metals have strong toxic effects in humans and can act as immunoregulatory factors. The purpose o... more Metals have strong toxic effects in humans and can act as immunoregulatory factors. The purpose of our study was to determine whether the concentrations of metals are associated with the clinical course of nasal polyposis (NP). We measured the concentrations of 10 metals (Zn, Mn, Se, Fe, Cr, Ni, Pb, Al, Cd, and Cu) in 58 patients with NP, and 29 controls with a healthy nasal mucosa. We used electron microscopy to compare the ultrastructural features of the nasal mucosa between NP patients and healthy controls. Concentrations of metals in nasal polyps and healthy mucosa were determined by mass spectrometry. Transmission electron microscopic (TEM) and scanning electron microscopic (SEM) images of the nasal mucosa were obtained. The mean tissue concentrations of all 10 metals were significantly lower in NP patients than in healthy controls (P < 0.001). Tissue concentrations of each metal were lower in stages III and IV NP than in stages I and II NP, although the differences were not...
Cells, 2020
Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging ... more Natural cosmetic products have recently re-emerged as a novel tool able to counteract skin aging and skin related damages. In addition, recently achieved progress in nanomedicine opens a novel approach yielding from combination of modern nanotechnology with traditional treatment for innovative pharmacotherapeutics. In the present study, we investigated the antiaging effect of a pretreatment with Myrtus communis natural extract combined with a polycaprolactone nanofibrous scaffold (NanoPCL-M) on skin cell populations exposed to UV. We set up a novel model of skin on a bioreactor mimicking a crosstalk between keratinocytes, stem cells and fibroblasts, as in skin. Beta-galactosidase assay, indicating the amount of senescent cells, and viability assay, revealed that fibroblasts and stem cells pretreated with NanoPCL-M and then exposed to UV are superimposable to control cells, untreated and unexposed to UV damage. On the other hand, cells only exposed to UV stress, without NanoPCL-M pre...
Cells, 2021
Tissue homeostasis mainly depends on the activity of stem cells to replace damaged elements and r... more Tissue homeostasis mainly depends on the activity of stem cells to replace damaged elements and restore tissue functions. Within this context, mesenchymal stem cells and fibroblasts are essential for maintaining tissue homeostasis in skin, in particular in the dermis. Modifications in collagen fibers are able to affect stem cell features. Skin properties can be significantly reduced after injuries or with aging, and stem cell niches, mainly comprising extracellular matrix (ECM), may be compromised. To this end, specific molecules can be administrated to prevent the aging process induced by UV exposure in the attempt to maintain a youngness phenotype. NanoPCL-M is a novel nanodevice able to control delivery of Mediterranean plant myrtle (Myrtus communis L.) extracts. In particular, we previously described that myrtle extracts, rich in bioactive molecules and nutraceuticals, were able to counteract senescence in adipose derived stem cells. In this study, we analyzed the effect of Nano...
Nature Communications, 2020
The island of Sardinia has been of particular interest to geneticists for decades. The current mo... more The island of Sardinia has been of particular interest to geneticists for decades. The current model for Sardinia’s genetic history describes the island as harboring a founder population that was established largely from the Neolithic peoples of southern Europe and remained isolated from later Bronze Age expansions on the mainland. To evaluate this model, we generate genome-wide ancient DNA data for 70 individuals from 21 Sardinian archaeological sites spanning the Middle Neolithic through the Medieval period. The earliest individuals show a strong affinity to western Mediterranean Neolithic populations, followed by an extended period of genetic continuity on the island through the Nuragic period (second millennium BCE). Beginning with individuals from Phoenician/Punic sites (first millennium BCE), we observe spatially-varying signals of admixture with sources principally from the eastern and northern Mediterranean. Overall, our analysis sheds light on the genetic history of Sardini...
Radiology Case Reports, 2019
The craniovertebral junction is a unique part of the somite-derived axial skeleton. The absence o... more The craniovertebral junction is a unique part of the somite-derived axial skeleton. The absence or hypoplasia of the posterior arch of C1 is frequently associated with compensatory hypertrophy of the anterior arch of C1 and of the spinous process of C2. Here, we report a patient with agenesis of the posterior arch of C1 without neurologic deficits. Our patient presented with complex alterations of the craniovertebral junction that involved interactions between the condyles, clivus, atlas, and epistropheus. To our knowledge, dislocation of the odontoid process above the Chamberlain line, including cranial migration of the anterior arch of C1, has not been reported in the literature.
Radiology Case Reports, 2019
Klippel-Feil syndrome is a congenital malformation characterized by the fusion of at least 2 cerv... more Klippel-Feil syndrome is a congenital malformation characterized by the fusion of at least 2 cervical vertebrae. It may occur in association with other clinical syndromes and disorders. We describe a case of prenatal diagnosis of a Klippel-Feil syndrome with Dandy-Walker malformation, and spina bifida, proved by ultrasound examination. A postmortem x-ray and autopsy were performed in a female fetus of 16 + 6 weeks of gestation: several malformations have been discovered. To the best of our knowledge, no similar cases have been reported in the medical literature. This case report underscores the importance of a careful ultrasound screening during pregnancy for an adequate diagnostic and therapeutic management.
Anatomical Sciences Education, 2019
the Achille's heel of medical students. A systematic analysis of educational strategies for the t... more the Achille's heel of medical students. A systematic analysis of educational strategies for the teaching of neuroanatomy. Anatomical Sciences Education 13 (1) , pp.
Advances in Infectious Diseases, 2017
Malaria has been an important disease throughout the history, particularly in the Mediterranean. ... more Malaria has been an important disease throughout the history, particularly in the Mediterranean. The island of Sardinia (Italy) was characterized by severe endemic malaria from antiquity until its eradication in 1950. It provides an exceptional and unique site to examine the history of the disease and its effects on human biology and ecology. Endemic malaria can cause chronic hemolytic anemias that may result in osteological signatures. This paper examines these markers, Porotic Hyperostosis (PH) and Cribra Orbitalia (CO), which are identified from skeletons uncovered in archaeological excavations in Northern Sardinia. Previous hypotheses based on historical references and genetic data suggest that the disease was absent on the island in prehistory and was imported, probably by slaves, during the Carthaginian period (6th to 3rd century BC). This article reports on the paleontological analysis of 283 skeletons excavated from seven archeological sites dating from 4700 BP to 1582 AD. Osteological remains were examined for markers CO and PH as a proxy variable for malaria. The findings support previous hypotheses about history of malaria over time.
II BIOANTHROPOLOGICAL MEETING: Life, death and in between
Rita Maria SERRA, Valeria POMPONI, Daniela ROVINA, Alessandra LA FRAGOLA, Andrea MONTELLA, Maria ... more Rita Maria SERRA, Valeria POMPONI, Daniela ROVINA, Alessandra LA FRAGOLA,
Andrea MONTELLA, Maria Alessandra SOTGIU, Vittorio MAZZARELLO, Antonio
BRUNETTI, Pasquale BANDIERA