Alessia Bertero | Università degli Studi di Torino (original) (raw)

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Papers by Alessia Bertero

Research paper thumbnail of In vitro effects of two environmental toxicants, beauvericin and glyphosate in Roundup, on cell numbers and steroidogenesis of bovine ovarian cells

Veterinary research communications, Apr 1, 2024

Research paper thumbnail of In vitro effects of different ozone preparations on microorganisms responsible for endometritis in the mare

Theriogenology, Feb 1, 2024

Research paper thumbnail of Species-specific models in toxicology: in vitro epithelial barriers

Environmental Toxicology and Pharmacology, Aug 1, 2019

Species-specific in vitro epithelial barriers represent interesting predictive tools for risk ass... more Species-specific in vitro epithelial barriers represent interesting predictive tools for risk assessment evaluation in toxicological studies. Moreover, these models could be applied either as stand-alone methods for the study of absorption, bioavailability, excretion, transport, effects of xenobiotics, or through an Integrated Testing Strategy. The aim of this review is to give a comprehensive overview of in vitro species-specific epithelial barrier models from bovine, dog and swine. Bovine mammary epithelial barrier as a fundamental instrument for the evaluation of the toxicant excretion, the blood brain barrier as a useful first approach in toxicological and pharmacological studies, the porcine intestinal barrier, the canine skin barrier, and finally the pulmonary barrier from bovine and swine species are described in this review.

Research paper thumbnail of Safety and Effects of a Commercial Ozone Foam Preparation on Endometrial Environment and Fertility of Mares

Journal of Equine Veterinary Science, Feb 1, 2023

Research paper thumbnail of Beauvericin and enniatin B1: an in vitro integrated approach for risk assessment

Toxicology Letters, Sep 1, 2021

Research paper thumbnail of Influence of Intrauterine Fluid Detection, Number of Transfers and Age of the Recipient on Pregnancy Rate and Early Embryonic Loss in a Commercial Embryo Transfer Program

Animals

The selection of the recipient mare is one of the most important factors involved in the success ... more The selection of the recipient mare is one of the most important factors involved in the success of equine embryo transfer. The aim of this study was to determine whether the age of the recipient, the number of transfers and the detection of intrauterine fluid during the follicular phase or after ovulation can affect pregnancy rate at 14 and 45 days (PR 14 and PR 45) or early embryonic loss (EEL). A total of 1222 ETs were included in the study. Mares receiving the first embryo of the year had a higher PR 14 and 45 days compared to mares at the third transfer (78.8% and 70.1% vs. 65.6% and 54.1%, respectively). The detection of intrauterine fluid post ovulation negatively affected PR 14 (60.5% vs. 77.6%) and should therefore be considered an abnormal finding, probably being a sign of uterine inflammation or delayed uterine clearance. On the contrary, the age of the recipient mare and detection of fluid during follicular phase did not affect PR 14. Only the age of the recipient mare i...

Research paper thumbnail of Challenging the Hypothesis of in Utero Microbiota Acquisition in Healthy Canine and Feline Pregnancies at Term: Preliminary Data

Veterinary Sciences

At present, there are no data on the presence of bacteria in healthy canine and feline pregnancie... more At present, there are no data on the presence of bacteria in healthy canine and feline pregnancies at term. Here, we investigated the uterine microbiome in bitches (n = 5) and queens (n = 3) undergoing elective cesarean section in two facilities. Samples included swabs from the endometrium, amniotic fluid, and meconium, and environmental swabs of the surgical tray as controls. Culture and 16S rRNA gene sequencing were used to investigate the presence of bacteria. Culture was positive for 34.3% of samples (uterus n = 3, amniotic fluid n = 2, meconium n = 4, controls n = 0), mostly with low growth of common contaminant bacteria. With sequencing techniques, the bacterial abundance was significantly lower than in environmental controls (p < 0.05). Sequencing results showed a species-specific pattern, and significant differences between canine and feline bacterial populations were found at order, family, and genus level. No differences were found in alpha and beta diversities between ...

Research paper thumbnail of P12-24 Emerging mycotoxins: in vitro transport on IPEC-J2 intestinal barrier

Research paper thumbnail of Virtual class on alternative methods in toxicology

World Congress on Alternatives and Animal Use in the Life Sciences, 2021

Research paper thumbnail of Testing mammary gland secretions to predict foaling in jennies

Italian Journal of Animal Science

Research paper thumbnail of The ROle of the University in Teaching and Education on ALTernative : ROUTE ALT

Research paper thumbnail of Testing mammary gland secrections to predict foaling in jennies

Research paper thumbnail of The evolution of dam-litter microbial flora from birth to 60 days of age

BMC Veterinary Research, 2022

Background Early bacterial colonization in puppies is still a poorly understood phenomenon. Altho... more Background Early bacterial colonization in puppies is still a poorly understood phenomenon. Although the topic is of considerable interest, a big gap in knowledge still exists on the understanding of timing and features of neonatal gut colonization. Thence, the purpose of this study was to evaluate the relationship between dam and litter microbial flora, in vaginally delivered puppies, from birth to two months of age. Bacteria were identified using MALDI-TOF, an accurate and sensitive method, and cluster analysis of data provided a new insight on the investigated topic. Methods Six dam-litter units of two medium size breeds were enrolled in the study. Vaginal and colostrum/milk samples were collected from dams after delivery and 48h post-partum, while rectal samples were taken from dams and puppies after delivery and at day 2, 30 and 60 (T2, T30 and T60, respectively) post-partum. Bacterial isolation and identification were performed following standard techniques, then the data were...

Research paper thumbnail of Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and brain in vitro barriers: A review

Food and Chemical Toxicology, 2018

Review Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and bra... more Review Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and brain in vitro barriers: a review.

Research paper thumbnail of Evaluation of some gestational parameters in jennies kept to produce milk for human consuption

Research paper thumbnail of Possibilities and Limits of Hematic Indicators as Marker of Inflammatory Uterine Pathologies in Piedmontese Cows

Research paper thumbnail of From 3Rs to 3D: In vitro alternative models for replacement

Research paper thumbnail of {"__content__"=>" Molds and Mycotoxins: Potential Species-Specific Effects.", "i"=>{"__content__"=>"Fusarium"}}

Toxins, Jan 15, 2018

This review summarizes the information on biochemical and biological activity of the main mycotox... more This review summarizes the information on biochemical and biological activity of the main mycotoxins, focusing on toxicological aspects in terms of species-specific effects. Both in vitro and in vivo studies have centered on the peculiarity of the responses to mycotoxins, demonstrating that toxicokinetics, bioavailability and the mechanisms of action of these substances vary depending on the species involved, but additional studies are needed to better understand the specific responses. The aim of this review is to summarize the toxicological responses of the main species affected by mycotoxins.

Research paper thumbnail of Assessment of the temperature cut-off point by a commercial intravaginal device to predict parturition in Piedmontese beef cows

Theriogenology, 2018

Dystocic parturitions have an adverse impact on animal productivity and therefore the profitabili... more Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows. A commercial intravaginal electronic device (Medria Vel'Phone) with a sensor that measures the IVT every 12 h was used. Piedmontese cows (n = 211; 27 primiparous and 184 multiparous) were included in this study. One-way analysis of variance was used to assess the temperature differences at 0, 12, 24, 36, 48 and 60 h before parturition. Receiving operator characteristic cur...

Research paper thumbnail of iPSCs and toxicology

Current Topics in iPSCs Technology, 2022

Research paper thumbnail of In vitro effects of two environmental toxicants, beauvericin and glyphosate in Roundup, on cell numbers and steroidogenesis of bovine ovarian cells

Veterinary research communications, Apr 1, 2024

Research paper thumbnail of In vitro effects of different ozone preparations on microorganisms responsible for endometritis in the mare

Theriogenology, Feb 1, 2024

Research paper thumbnail of Species-specific models in toxicology: in vitro epithelial barriers

Environmental Toxicology and Pharmacology, Aug 1, 2019

Species-specific in vitro epithelial barriers represent interesting predictive tools for risk ass... more Species-specific in vitro epithelial barriers represent interesting predictive tools for risk assessment evaluation in toxicological studies. Moreover, these models could be applied either as stand-alone methods for the study of absorption, bioavailability, excretion, transport, effects of xenobiotics, or through an Integrated Testing Strategy. The aim of this review is to give a comprehensive overview of in vitro species-specific epithelial barrier models from bovine, dog and swine. Bovine mammary epithelial barrier as a fundamental instrument for the evaluation of the toxicant excretion, the blood brain barrier as a useful first approach in toxicological and pharmacological studies, the porcine intestinal barrier, the canine skin barrier, and finally the pulmonary barrier from bovine and swine species are described in this review.

Research paper thumbnail of Safety and Effects of a Commercial Ozone Foam Preparation on Endometrial Environment and Fertility of Mares

Journal of Equine Veterinary Science, Feb 1, 2023

Research paper thumbnail of Beauvericin and enniatin B1: an in vitro integrated approach for risk assessment

Toxicology Letters, Sep 1, 2021

Research paper thumbnail of Influence of Intrauterine Fluid Detection, Number of Transfers and Age of the Recipient on Pregnancy Rate and Early Embryonic Loss in a Commercial Embryo Transfer Program

Animals

The selection of the recipient mare is one of the most important factors involved in the success ... more The selection of the recipient mare is one of the most important factors involved in the success of equine embryo transfer. The aim of this study was to determine whether the age of the recipient, the number of transfers and the detection of intrauterine fluid during the follicular phase or after ovulation can affect pregnancy rate at 14 and 45 days (PR 14 and PR 45) or early embryonic loss (EEL). A total of 1222 ETs were included in the study. Mares receiving the first embryo of the year had a higher PR 14 and 45 days compared to mares at the third transfer (78.8% and 70.1% vs. 65.6% and 54.1%, respectively). The detection of intrauterine fluid post ovulation negatively affected PR 14 (60.5% vs. 77.6%) and should therefore be considered an abnormal finding, probably being a sign of uterine inflammation or delayed uterine clearance. On the contrary, the age of the recipient mare and detection of fluid during follicular phase did not affect PR 14. Only the age of the recipient mare i...

Research paper thumbnail of Challenging the Hypothesis of in Utero Microbiota Acquisition in Healthy Canine and Feline Pregnancies at Term: Preliminary Data

Veterinary Sciences

At present, there are no data on the presence of bacteria in healthy canine and feline pregnancie... more At present, there are no data on the presence of bacteria in healthy canine and feline pregnancies at term. Here, we investigated the uterine microbiome in bitches (n = 5) and queens (n = 3) undergoing elective cesarean section in two facilities. Samples included swabs from the endometrium, amniotic fluid, and meconium, and environmental swabs of the surgical tray as controls. Culture and 16S rRNA gene sequencing were used to investigate the presence of bacteria. Culture was positive for 34.3% of samples (uterus n = 3, amniotic fluid n = 2, meconium n = 4, controls n = 0), mostly with low growth of common contaminant bacteria. With sequencing techniques, the bacterial abundance was significantly lower than in environmental controls (p < 0.05). Sequencing results showed a species-specific pattern, and significant differences between canine and feline bacterial populations were found at order, family, and genus level. No differences were found in alpha and beta diversities between ...

Research paper thumbnail of P12-24 Emerging mycotoxins: in vitro transport on IPEC-J2 intestinal barrier

Research paper thumbnail of Virtual class on alternative methods in toxicology

World Congress on Alternatives and Animal Use in the Life Sciences, 2021

Research paper thumbnail of Testing mammary gland secretions to predict foaling in jennies

Italian Journal of Animal Science

Research paper thumbnail of The ROle of the University in Teaching and Education on ALTernative : ROUTE ALT

Research paper thumbnail of Testing mammary gland secrections to predict foaling in jennies

Research paper thumbnail of The evolution of dam-litter microbial flora from birth to 60 days of age

BMC Veterinary Research, 2022

Background Early bacterial colonization in puppies is still a poorly understood phenomenon. Altho... more Background Early bacterial colonization in puppies is still a poorly understood phenomenon. Although the topic is of considerable interest, a big gap in knowledge still exists on the understanding of timing and features of neonatal gut colonization. Thence, the purpose of this study was to evaluate the relationship between dam and litter microbial flora, in vaginally delivered puppies, from birth to two months of age. Bacteria were identified using MALDI-TOF, an accurate and sensitive method, and cluster analysis of data provided a new insight on the investigated topic. Methods Six dam-litter units of two medium size breeds were enrolled in the study. Vaginal and colostrum/milk samples were collected from dams after delivery and 48h post-partum, while rectal samples were taken from dams and puppies after delivery and at day 2, 30 and 60 (T2, T30 and T60, respectively) post-partum. Bacterial isolation and identification were performed following standard techniques, then the data were...

Research paper thumbnail of Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and brain in vitro barriers: A review

Food and Chemical Toxicology, 2018

Review Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and bra... more Review Fusarium mycotoxins and in vitro species-specific approach with porcine intestinal and brain in vitro barriers: a review.

Research paper thumbnail of Evaluation of some gestational parameters in jennies kept to produce milk for human consuption

Research paper thumbnail of Possibilities and Limits of Hematic Indicators as Marker of Inflammatory Uterine Pathologies in Piedmontese Cows

Research paper thumbnail of From 3Rs to 3D: In vitro alternative models for replacement

Research paper thumbnail of {"__content__"=>" Molds and Mycotoxins: Potential Species-Specific Effects.", "i"=>{"__content__"=>"Fusarium"}}

Toxins, Jan 15, 2018

This review summarizes the information on biochemical and biological activity of the main mycotox... more This review summarizes the information on biochemical and biological activity of the main mycotoxins, focusing on toxicological aspects in terms of species-specific effects. Both in vitro and in vivo studies have centered on the peculiarity of the responses to mycotoxins, demonstrating that toxicokinetics, bioavailability and the mechanisms of action of these substances vary depending on the species involved, but additional studies are needed to better understand the specific responses. The aim of this review is to summarize the toxicological responses of the main species affected by mycotoxins.

Research paper thumbnail of Assessment of the temperature cut-off point by a commercial intravaginal device to predict parturition in Piedmontese beef cows

Theriogenology, 2018

Dystocic parturitions have an adverse impact on animal productivity and therefore the profitabili... more Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows. A commercial intravaginal electronic device (Medria Vel'Phone) with a sensor that measures the IVT every 12 h was used. Piedmontese cows (n = 211; 27 primiparous and 184 multiparous) were included in this study. One-way analysis of variance was used to assess the temperature differences at 0, 12, 24, 36, 48 and 60 h before parturition. Receiving operator characteristic cur...

Research paper thumbnail of iPSCs and toxicology

Current Topics in iPSCs Technology, 2022