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Papers by Domenico Lafiandra

Research paper thumbnail of Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat

Theoretical and Applied Genetics, Nov 1, 1996

ABSTRACT

Research paper thumbnail of a-Type Prolamins Are Encoded by Genes on Chromosomes 4Ha and 6Ha ofHaynaldia villosa Schur (syn. Dasypy, um villosum L.)

Research paper thumbnail of PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus

Theoretical and Applied Genetics, 1994

Genes encoding high-molecular-weight (HMW) glutenin subunits, present in bread-wheat lines and cu... more Genes encoding high-molecular-weight (HMW) glutenin subunits, present in bread-wheat lines and cultivars, were studied by RFLP (restriction fragment length polymorphism) and PCR (polymerase chain reaction) analyses. In particular, allelic subunits of the x-or y-type, encoded at the Glu-D1 locus present on the long arm of chromosome 1D, were investigated. The variation in size, observed in different allelic subunits, is mainly due to variation in the length of the central repetitive domain, typical of these proteins. Deletions or duplications, probably caused by unequal crossingover, have given rise to the size heterogeneity currently observed. The possibility of using the PCR technique for a detailed analysis of HMW glutenin genes in order to obtain a more accurate estimation of the molecular weight of their encoded subunits, and the detection of unexpressed genes, is also described.

Research paper thumbnail of Wheat Protein Structure and Functionality-Purification and Characterisation of 1Bx and 1By High Mr Glutenin Subunits from Durum Wheat Cultivar Lira

Research paper thumbnail of Transfer of Soft Kernel Texture from Triticum aestivum to Durum Wheat, Triticum turgidum ssp. durum

Crop Science, 2011

Durum wheat (Triticum turgidum ssp. durum) is a leading cereal grain whose primary use is the pro... more Durum wheat (Triticum turgidum ssp. durum) is a leading cereal grain whose primary use is the production of semolina and pasta. Its rich culinary relationship to humans is related, in part, to its very hard kernel texture. This very hard texture is due to the loss of the Puroindoline genes that were eliminated during the allopolyploid formation of T. turgidum approximately 0.5 million years ago. In the present report, we describe the transfer of the Puroindoline genes through ph1b‐mediated homoeologous recombination. Puroindoline a and Puroindoline b were successfully recombined (translocated) from chromosome 5D of the soft wheat (T. aestivum) variety Chinese Spring into cv. Langdon durum using a Langdon 5D(5B) disomic substitution line. Although initial recombination lines were highly unstable, recurrent backcrossing into Svevo durum cultivar produced stable lines that segregated in a normal 1:2:1 soft:heterozygous:very hard ratio. The final backcross (BC3) Svevo line produced unif...

Research paper thumbnail of Wheat gluten

... Jacopo Bartholomew Beccari (top) was Professor of Chemistry at the University of Bologna when... more ... Jacopo Bartholomew Beccari (top) was Professor of Chemistry at the University of Bologna when he described the isolation of gluten in 1745. ... G. Pastori, L. Rooke, S. Steele, G. He, P. Tosi, R. D'Ovidio, F. Bekes, H. Darlington, J. Napier, R. Fido, AS Tatham, P. Barcelo and P ...

Research paper thumbnail of Soft Kernel Durum Wheat—A New Bakery Ingredient?

Cereal Foods World, 2015

1 Names are necessary to report factually on available data; however, the USDA neither guarantees... more 1 Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable.

Research paper thumbnail of Published full paper

Research paper thumbnail of Isogenic bread wheat lines differing in number and type of high Mr. glutenin subunits

Research paper thumbnail of Biochemical and Functional Studies of Wheat Isolines Containing Single Typehigh Molecular Weight Glutenin Subunits (HMW-GS)

The Gluten Proteins, 2007

Research paper thumbnail of Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat

Theoretical and Applied Genetics, 1996

A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to posse... more A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to possess a novel high-molecular-weight glutenin subunit (HMW-GS). The subunit has a very slow electrophoretic mobility as revealed by SDS-PAGE, and its molecular weight is comparable to that of the highest molecular weight glutenin subunit ("2.2" encoded in the D-genome) reported so far in hexaploid wheat varieties and landraces of Japanese origin. Evidence obtained from (PCR) gene amplification studies using the primers specific for Glu-1 loci proved that the gene coding for this novel subunit belongs to the GIu-A1 locus located on the long arm of chromosome 1A. Digestion of the amplified gene (PCR product) with restriction enzymes indicated that the novel gene differs from prevailing Glu-A1 alleles (null, 1 and 2*) by an extra DNA fragment of approximately 600 base pairs. The results also indicated that the novel subunit is most probably a derivative of subunit 2* that has very likely incorporated the 600-bp fragment following a process of unequal crossing over. The present findings were further substantiated by reserved phase high performance liquid chromatography (RP-HPLC) analysis.

Research paper thumbnail of New Data Supporting High Mr Glutenin Subunit 5 as the Determinant of Quality Differences among the Pairs 5 + 10 vs. 2 + 12

Journal of Cereal Science, 1993

Research paper thumbnail of Assessment of genetic variability in hexaploid wheat landraces of Pakistan based on polymorphism for HMW glutenin subunits

Genetic Resources and Crop Evolution, 1996

Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic... more Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic variability in terms of high molecular weight (HMW) glutenin subunits as revealed by SDS-PAGE. The germplasm appeared to be diverse and unique on the basis of HMW glutenin subunit compositions_ Out of 24 alleles detected at all the Glu-1 loci, four belonged to Glu-Al, 12 to Glu-B1 and eight to Glu-D1 locus. The number of novel HMW glutenin subunits detected were 1,4 and 6 at the three loci (Glu-A1, Glu-Bl, Glu-D1), respectively. The frequency distribution patterns of 24 allelic variants detected at the three Glu-1 loci in 1080 samples analysed for 60 accessions were determined both on the basis of individual accessions and on the basis of regions (accessions pooled across the regions). One allele ("null") at the Glu-A1 locus, three alleles (17 + 18, 7 + 8, 14) at the Glu-B1 locus and, two alleles (2 + 12 and 2** + 1U) at the Glu-D1 locus were found most frequently distributed in the 60 populations. Maximum variation was observed in the Baluchistan and Gilgit regions of Pakistan in terms of distribution of novel Glu-1 alleles. A higher gene diversity was observed between the populations as compared to the gene diversity within the populations while, a reverse pattern of gene diversity was observed when populations were pooled across the regions (higher within the regions than between the regions). A data base has been generated in this study which could be expanded and usefully exploited for cultivar development or management of gene bank accessions.

Research paper thumbnail of Chromosomal location of genes encoding gliadins and high molecular weight glutenin subunits in Aegilops searsii

Research paper thumbnail of Electrophoretic and Chromatographic Characterization of Glu-A1 Encoded High M~ r Glutenin Subunits

Research paper thumbnail of Use of mutagenesis to induce novel allelic variation for genes involved in starch biosynthesis

Research paper thumbnail of Waxy protein variation in tetraploid and hexaploid wheats

Research paper thumbnail of Seed storage proteins and wheat genetic resources

Research paper thumbnail of Combining bioactive components with conventional targets in plant breeding programmes

Research paper thumbnail of Studies of High Molecular Weight Glutenin Subunits and Their Encoding Genes

Improvement of Cereal Quality by Genetic Engineering, 1994

Our knowledge of the biochemical and genetical aspects of high molecular weight (HMW) glutenin su... more Our knowledge of the biochemical and genetical aspects of high molecular weight (HMW) glutenin subunits has been possible through the extensive use of electrophoretic techniques and of SDS-PAGE in particular; in fact by using this technique the existence of allelic variation at each of the three Glu-1 loci encoding HMW glutenin subunits in bread wheat and the influence they have in determining qualitative properties have been firmly established (Payne, 1987). Use of different and more sophisticated biochemical techniques, such as reversed-phase high performance liquid chromatography (RP-HPLC) or molecular studies conducted with new tools, such as the polymerase chain reaction (PCR) and nucleotide sequence analysis, are constantly supplying more information on these proteins and their encoding genes.

Research paper thumbnail of Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat

Theoretical and Applied Genetics, Nov 1, 1996

ABSTRACT

Research paper thumbnail of a-Type Prolamins Are Encoded by Genes on Chromosomes 4Ha and 6Ha ofHaynaldia villosa Schur (syn. Dasypy, um villosum L.)

Research paper thumbnail of PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus

Theoretical and Applied Genetics, 1994

Genes encoding high-molecular-weight (HMW) glutenin subunits, present in bread-wheat lines and cu... more Genes encoding high-molecular-weight (HMW) glutenin subunits, present in bread-wheat lines and cultivars, were studied by RFLP (restriction fragment length polymorphism) and PCR (polymerase chain reaction) analyses. In particular, allelic subunits of the x-or y-type, encoded at the Glu-D1 locus present on the long arm of chromosome 1D, were investigated. The variation in size, observed in different allelic subunits, is mainly due to variation in the length of the central repetitive domain, typical of these proteins. Deletions or duplications, probably caused by unequal crossingover, have given rise to the size heterogeneity currently observed. The possibility of using the PCR technique for a detailed analysis of HMW glutenin genes in order to obtain a more accurate estimation of the molecular weight of their encoded subunits, and the detection of unexpressed genes, is also described.

Research paper thumbnail of Wheat Protein Structure and Functionality-Purification and Characterisation of 1Bx and 1By High Mr Glutenin Subunits from Durum Wheat Cultivar Lira

Research paper thumbnail of Transfer of Soft Kernel Texture from Triticum aestivum to Durum Wheat, Triticum turgidum ssp. durum

Crop Science, 2011

Durum wheat (Triticum turgidum ssp. durum) is a leading cereal grain whose primary use is the pro... more Durum wheat (Triticum turgidum ssp. durum) is a leading cereal grain whose primary use is the production of semolina and pasta. Its rich culinary relationship to humans is related, in part, to its very hard kernel texture. This very hard texture is due to the loss of the Puroindoline genes that were eliminated during the allopolyploid formation of T. turgidum approximately 0.5 million years ago. In the present report, we describe the transfer of the Puroindoline genes through ph1b‐mediated homoeologous recombination. Puroindoline a and Puroindoline b were successfully recombined (translocated) from chromosome 5D of the soft wheat (T. aestivum) variety Chinese Spring into cv. Langdon durum using a Langdon 5D(5B) disomic substitution line. Although initial recombination lines were highly unstable, recurrent backcrossing into Svevo durum cultivar produced stable lines that segregated in a normal 1:2:1 soft:heterozygous:very hard ratio. The final backcross (BC3) Svevo line produced unif...

Research paper thumbnail of Wheat gluten

... Jacopo Bartholomew Beccari (top) was Professor of Chemistry at the University of Bologna when... more ... Jacopo Bartholomew Beccari (top) was Professor of Chemistry at the University of Bologna when he described the isolation of gluten in 1745. ... G. Pastori, L. Rooke, S. Steele, G. He, P. Tosi, R. D'Ovidio, F. Bekes, H. Darlington, J. Napier, R. Fido, AS Tatham, P. Barcelo and P ...

Research paper thumbnail of Soft Kernel Durum Wheat—A New Bakery Ingredient?

Cereal Foods World, 2015

1 Names are necessary to report factually on available data; however, the USDA neither guarantees... more 1 Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable.

Research paper thumbnail of Published full paper

Research paper thumbnail of Isogenic bread wheat lines differing in number and type of high Mr. glutenin subunits

Research paper thumbnail of Biochemical and Functional Studies of Wheat Isolines Containing Single Typehigh Molecular Weight Glutenin Subunits (HMW-GS)

The Gluten Proteins, 2007

Research paper thumbnail of Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat

Theoretical and Applied Genetics, 1996

A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to posse... more A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to possess a novel high-molecular-weight glutenin subunit (HMW-GS). The subunit has a very slow electrophoretic mobility as revealed by SDS-PAGE, and its molecular weight is comparable to that of the highest molecular weight glutenin subunit ("2.2" encoded in the D-genome) reported so far in hexaploid wheat varieties and landraces of Japanese origin. Evidence obtained from (PCR) gene amplification studies using the primers specific for Glu-1 loci proved that the gene coding for this novel subunit belongs to the GIu-A1 locus located on the long arm of chromosome 1A. Digestion of the amplified gene (PCR product) with restriction enzymes indicated that the novel gene differs from prevailing Glu-A1 alleles (null, 1 and 2*) by an extra DNA fragment of approximately 600 base pairs. The results also indicated that the novel subunit is most probably a derivative of subunit 2* that has very likely incorporated the 600-bp fragment following a process of unequal crossing over. The present findings were further substantiated by reserved phase high performance liquid chromatography (RP-HPLC) analysis.

Research paper thumbnail of New Data Supporting High Mr Glutenin Subunit 5 as the Determinant of Quality Differences among the Pairs 5 + 10 vs. 2 + 12

Journal of Cereal Science, 1993

Research paper thumbnail of Assessment of genetic variability in hexaploid wheat landraces of Pakistan based on polymorphism for HMW glutenin subunits

Genetic Resources and Crop Evolution, 1996

Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic... more Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic variability in terms of high molecular weight (HMW) glutenin subunits as revealed by SDS-PAGE. The germplasm appeared to be diverse and unique on the basis of HMW glutenin subunit compositions_ Out of 24 alleles detected at all the Glu-1 loci, four belonged to Glu-Al, 12 to Glu-B1 and eight to Glu-D1 locus. The number of novel HMW glutenin subunits detected were 1,4 and 6 at the three loci (Glu-A1, Glu-Bl, Glu-D1), respectively. The frequency distribution patterns of 24 allelic variants detected at the three Glu-1 loci in 1080 samples analysed for 60 accessions were determined both on the basis of individual accessions and on the basis of regions (accessions pooled across the regions). One allele ("null") at the Glu-A1 locus, three alleles (17 + 18, 7 + 8, 14) at the Glu-B1 locus and, two alleles (2 + 12 and 2** + 1U) at the Glu-D1 locus were found most frequently distributed in the 60 populations. Maximum variation was observed in the Baluchistan and Gilgit regions of Pakistan in terms of distribution of novel Glu-1 alleles. A higher gene diversity was observed between the populations as compared to the gene diversity within the populations while, a reverse pattern of gene diversity was observed when populations were pooled across the regions (higher within the regions than between the regions). A data base has been generated in this study which could be expanded and usefully exploited for cultivar development or management of gene bank accessions.

Research paper thumbnail of Chromosomal location of genes encoding gliadins and high molecular weight glutenin subunits in Aegilops searsii

Research paper thumbnail of Electrophoretic and Chromatographic Characterization of Glu-A1 Encoded High M~ r Glutenin Subunits

Research paper thumbnail of Use of mutagenesis to induce novel allelic variation for genes involved in starch biosynthesis

Research paper thumbnail of Waxy protein variation in tetraploid and hexaploid wheats

Research paper thumbnail of Seed storage proteins and wheat genetic resources

Research paper thumbnail of Combining bioactive components with conventional targets in plant breeding programmes

Research paper thumbnail of Studies of High Molecular Weight Glutenin Subunits and Their Encoding Genes

Improvement of Cereal Quality by Genetic Engineering, 1994

Our knowledge of the biochemical and genetical aspects of high molecular weight (HMW) glutenin su... more Our knowledge of the biochemical and genetical aspects of high molecular weight (HMW) glutenin subunits has been possible through the extensive use of electrophoretic techniques and of SDS-PAGE in particular; in fact by using this technique the existence of allelic variation at each of the three Glu-1 loci encoding HMW glutenin subunits in bread wheat and the influence they have in determining qualitative properties have been firmly established (Payne, 1987). Use of different and more sophisticated biochemical techniques, such as reversed-phase high performance liquid chromatography (RP-HPLC) or molecular studies conducted with new tools, such as the polymerase chain reaction (PCR) and nucleotide sequence analysis, are constantly supplying more information on these proteins and their encoding genes.