Marc Marci | Aix-Marseille University (original) (raw)
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Papers by Marc Marci
PLOS Medicine, 2006
We analyzed the temporal changes in global transcript levels in response to hypoxia in primary re... more We analyzed the temporal changes in global transcript levels in response to hypoxia in primary renal proximal tubule epithelial cells, breast epithelial cells, smooth muscle cells, and endothelial cells with DNA microarrays. The extent of the transcriptional response to hypoxia was greatest in the renal tubule cells. This heightened response was associated with a uniquely high level of HIF-1a RNA in renal cells, and it could be diminished by reducing HIF-1a expression via RNA interference. A gene-expression signature of the hypoxia response, derived from our studies of cultured mammary and renal tubular epithelial cells, showed coordinated variation in several human cancers, and was a strong predictor of clinical outcomes in breast and ovarian cancers. In an analysis of a large, published gene-expression dataset from breast cancers, we found that the prognostic information in the hypoxia signature was virtually independent of that provided by the previously reported wound signature and more predictive of outcomes than any of the clinical parameters in current use.
Annals of Thoracic Surgery, 2004
The Cox-Maze III remains the gold standard for the surgical treatment of atrial fibrillation. How... more The Cox-Maze III remains the gold standard for the surgical treatment of atrial fibrillation. However, the “cut-and-sew” technique is time consuming and technically challenging. The pulmonary veins are the source of ectopy in the majority of patients with atrial fibrillation. The safety and efficacy of bipolar radiofrequency to electrically isolate the pulmonary veins was evaluated in a prospective multi-center trial.Beginning in January 2002, 30 patients at three medical centers underwent pulmonary vein isolation using bipolar radiofrequency and were followed for 6 months. Twenty-four of the patients also underwent a modified Cox-Maze III. Electrical isolation of the pulmonary veins was confirmed with intraoperative pacing. Pulmonary vein patency was assessed by magnetic resonance imaging or three-dimensional computed tomography in 15 patients at 1 month.Mean age was 60.9 ± 11.7 years. Nineteen patients had paroxysmal atrial fibrillation. All pulmonary veins were isolated in every patient. The left pulmonary veins underwent 3.0 ± 1.4 applications for a total of 26.4 ± 10.5 seconds. The right pulmonary veins underwent 2.8 ± 1.1 applications for a total of 26.3 ± 12.6 seconds. There was no operative mortality. At 1 month, imaging revealed no evidence of pulmonary vein stenosis. At 6 months, 96% of patients were in normal sinus rhythm.The use of bipolar radiofrequency for electrical isolation of pulmonary veins and to replace other Cox-Maze III incisions is safe and effective at controlling atrial fibrillation. This emerging technology may shorten and simplify the surgical management of atrial fibrillation.
Current Eye Research, 1986
The effect of optical blur on the steady-state (6, 8, 10 Hz) counterphase pattern electroretinogr... more The effect of optical blur on the steady-state (6, 8, 10 Hz) counterphase pattern electroretinogram (PERG) was measured in three normal cynomolgus monkeys. Blur was induced using lenses of -8.00 to +12.00 diopters, and monocular PERGs were recorded at a viewing distance of 0.32 meters. A substantial decrease in PERG amplitude occurred with a 0.51 cpd grating when +4.00 diopters of blur or greater were used. PERG amplitude to a 1.25 cpd grating decreased with lenses greater than +2.00 diopters. Minus lenses caused a steep decline in PERG amplitude of both spatial frequency gratings. These results indicate that refractive state has a significant effect upon the PERG and should be carefully considered in electrophysiologic testing of the primate visual system.
PLOS Medicine, 2006
We analyzed the temporal changes in global transcript levels in response to hypoxia in primary re... more We analyzed the temporal changes in global transcript levels in response to hypoxia in primary renal proximal tubule epithelial cells, breast epithelial cells, smooth muscle cells, and endothelial cells with DNA microarrays. The extent of the transcriptional response to hypoxia was greatest in the renal tubule cells. This heightened response was associated with a uniquely high level of HIF-1a RNA in renal cells, and it could be diminished by reducing HIF-1a expression via RNA interference. A gene-expression signature of the hypoxia response, derived from our studies of cultured mammary and renal tubular epithelial cells, showed coordinated variation in several human cancers, and was a strong predictor of clinical outcomes in breast and ovarian cancers. In an analysis of a large, published gene-expression dataset from breast cancers, we found that the prognostic information in the hypoxia signature was virtually independent of that provided by the previously reported wound signature and more predictive of outcomes than any of the clinical parameters in current use.
Annals of Thoracic Surgery, 2004
The Cox-Maze III remains the gold standard for the surgical treatment of atrial fibrillation. How... more The Cox-Maze III remains the gold standard for the surgical treatment of atrial fibrillation. However, the “cut-and-sew” technique is time consuming and technically challenging. The pulmonary veins are the source of ectopy in the majority of patients with atrial fibrillation. The safety and efficacy of bipolar radiofrequency to electrically isolate the pulmonary veins was evaluated in a prospective multi-center trial.Beginning in January 2002, 30 patients at three medical centers underwent pulmonary vein isolation using bipolar radiofrequency and were followed for 6 months. Twenty-four of the patients also underwent a modified Cox-Maze III. Electrical isolation of the pulmonary veins was confirmed with intraoperative pacing. Pulmonary vein patency was assessed by magnetic resonance imaging or three-dimensional computed tomography in 15 patients at 1 month.Mean age was 60.9 ± 11.7 years. Nineteen patients had paroxysmal atrial fibrillation. All pulmonary veins were isolated in every patient. The left pulmonary veins underwent 3.0 ± 1.4 applications for a total of 26.4 ± 10.5 seconds. The right pulmonary veins underwent 2.8 ± 1.1 applications for a total of 26.3 ± 12.6 seconds. There was no operative mortality. At 1 month, imaging revealed no evidence of pulmonary vein stenosis. At 6 months, 96% of patients were in normal sinus rhythm.The use of bipolar radiofrequency for electrical isolation of pulmonary veins and to replace other Cox-Maze III incisions is safe and effective at controlling atrial fibrillation. This emerging technology may shorten and simplify the surgical management of atrial fibrillation.
Current Eye Research, 1986
The effect of optical blur on the steady-state (6, 8, 10 Hz) counterphase pattern electroretinogr... more The effect of optical blur on the steady-state (6, 8, 10 Hz) counterphase pattern electroretinogram (PERG) was measured in three normal cynomolgus monkeys. Blur was induced using lenses of -8.00 to +12.00 diopters, and monocular PERGs were recorded at a viewing distance of 0.32 meters. A substantial decrease in PERG amplitude occurred with a 0.51 cpd grating when +4.00 diopters of blur or greater were used. PERG amplitude to a 1.25 cpd grating decreased with lenses greater than +2.00 diopters. Minus lenses caused a steep decline in PERG amplitude of both spatial frequency gratings. These results indicate that refractive state has a significant effect upon the PERG and should be carefully considered in electrophysiologic testing of the primate visual system.