nadim CASSIR | Aix-Marseille University (original) (raw)

Papers by nadim CASSIR

BMC infectious diseases, May 28, 2024

Microorganisms, Oct 10, 2023

$Research paper thumbnail of Refractory Pseudomonas aeruginosa Bronchopulmonary Infection After Lung Transplantation for Common Variable Immunodeficiency Despite Maximal Treatment Including IgM/IgA-Enriched Immunoglobulins and Bacteriophage Therapy$

Infection and Drug Resistance, Jun 1, 2023

Recipients transplanted for bronchiectasis in the context of a primary immune deficiency, such as... more Recipients transplanted for bronchiectasis in the context of a primary immune deficiency, such as common variable immunodeficiency, are at a high risk of severe infection in post-transplantation leading to poorer long-term outcomes than other transplant indications. In this report, we present a fatal case due to chronic Pseudomonas aeruginosa bronchopulmonary infection in a lung transplant recipient with common variable immunodeficiency despite successful eradication of an extensively drug-resistant (XDR) strain with IgM/IgA-enriched immunoglobulins and bacteriophage therapy. The fatal evolution despite a drastic adaptation of the immunosuppressive regimen and the maximal antibiotic therapy strategy raises the question of the contraindication of lung transplantation in such a context of primary immunodeficiency.

Standards in Genomic Sciences, Apr 4, 2014

Microorganisms, Dec 7, 2022

During SARS-CoV-2 infection, eosinopenia may reflect a hyperactive immune response. In this study... more During SARS-CoV-2 infection, eosinopenia may reflect a hyperactive immune response. In this study of hospitalized COVID-19 patients, we aimed to better understand the prognostic value of severe eosinopenia (absolute eosinophil count = 0 G/L) and decipher its underlying mechanisms. We retrospectively analyzed the records of COVID-19 patients hospitalized from March to June 2020 in three university hospitals in Marseille, France. We assessed the association between severe eosinopenia and a composite poor outcome in these patients, including the need for oxygen supplementation at >6 L/min, ICU admission, and in-hospital death. Among the 551 COVID-19 patients included in this study, severe eosinopenia was found in 228 (51%) of them on admission to hospital and was associated with a composite poor outcome using multivariate analysis (OR = 2.58; CI95 [1.77-3.75]; p < 0.0001). We found a significant association between the presence of severe eosinopenia on admission and the elevation in C-reactive protein, ferritin, IP-10, and suPAR. The histological findings in a series of 37 autopsies from patients who died from severe COVID-19 and presented with severe eosinopenia showed no pulmonary eosinophil trapping. Severe eosinopenia can be a reliable biomarker associated with a composite poor outcome in hospitalized COVID-19 adult patients. It may reflect the magnitude of immune hyperactivation during severe-to-critical COVID-19.

European Journal of Clinical Microbiology & Infectious Diseases, Oct 7, 2016

Archives De Pediatrie, Jun 1, 2012

Clinical Infectious Diseases, Jun 17, 2015

Journal of Medical Virology

A large outbreak of Monkeypox virus (MPXV) infections has arisen in May 2022 in nonendemic countr... more A large outbreak of Monkeypox virus (MPXV) infections has arisen in May 2022 in nonendemic countries. Here, we performed DNA metagenomics using next‐generation sequencing with Illumina or Nanopore technologies for clinical samples from MPXV‐infected patients diagnosed between June and July 2022. Classification of the MPXV genomes and determination of their mutational patterns were performed using Nextclade. Twenty‐five samples from 25 patients were studied. A MPXV genome was obtained for 18 patients, essentially from skin lesions and rectal swabbing. All 18 genomes were classified in clade IIb, lineage B.1, and we identified four B.1 sublineages (B.1.1, B.1.10, B.1.12, B.1.14). We detected a high number of mutations (range, 64−73) relatively to a 2018 Nigerian genome (genome GenBank Accession no. NC_063383.1), which were harbored by a large part of a set of 3184 MPXV genomes of lineage B.1 recovered from GenBank and Nextstrain; and we detected 35 mutations relatively to genome ON563...

Scientific Reports, 2021

Data on the relationship between antimicrobial resistance and mortality remain scarce, and this r... more Data on the relationship between antimicrobial resistance and mortality remain scarce, and this relationship needs to be investigated in intensive care units (ICUs). The aim of this study was to compare the ICU mortality rates between patients with ICU-acquired pneumonia due to highly antimicrobial-resistant (HAMR) bacteria and those with ICU-acquired pneumonia due to non-HAMR bacteria. We conducted a multicenter, retrospective cohort study using the French National Surveillance Network for Healthcare Associated Infection in ICUs (“REA-Raisin”) database, gathering data from 200 ICUs from January 2007 to December 2016. We assessed all adult patients who were hospitalized for at least 48 h and presented with ICU-acquired pneumonia caused by S. aureus, Enterobacteriaceae, P. aeruginosa, or A. baumannii. The association between pneumonia caused by HAMR bacteria and ICU mortality was analyzed using the whole sample and using a 1:2 matched sample. Among the 18,497 patients with at least o...

Médecine et Maladies Infectieuses, 2018

Introduction La prise en charge des patients colonises a EPC represente un challenge en terme de ... more Introduction La prise en charge des patients colonises a EPC represente un challenge en terme de sante publique et de prise en charge individuelle. Au cours d’une hospitalisation, ils relevent de prise en charge specifique, avec la mise en place de mesures renforcees (cohorting, equipe dediee, depistage des patients contacts) aux consequences economiques (surcout des politiques d’isolement et de depistage) et medicales (perte de chance par mise en second plan du motif initial d’hospitalisation). Via l’exemple d’une cohorte de patients colonises a EPC, nous illustrons la balance benefice/risque de ces mesures en soulignant le rationnel des politiques de cohorting, l’interet des mesures de decolonisation et la perte de chance associee a ces mesures. Materiels et methodes Etude descriptive d’une cohorte de 6 patients de octobre 2017 a fevrier 2018 pris en charge en service de Maladies infectieuses et tropicales (MIT) lors d’une epidemie de colonisation digestive a EPC (OXA48) en secteu...

New Microbes and New Infections, 2021

In 2020, as part of the diagnosis in IHU-Méditerranée Infection Institute in Marseille (France) w... more In 2020, as part of the diagnosis in IHU-Méditerranée Infection Institute in Marseille (France) we isolated the new bacterial strain Marseille-3630T from a 7-year-old girl blood specimen (= CSUR: Q3630). Matrix-assisted desorption ionisation time–of-flight mass spectrometry failed to identify this isolate. Analysis of the 16S rRNA gene and genome-to-genome comparison suggested that this taxon belongs to a novel bacterial species within the family Corynebacteriaceae in the phylum Actinobacteria. We described here its main phenotypic characteristics, genome sequence and annotation of Corynebacterium incognitum strain Marseille-3630T, a new member of the Corynebacterium genus, that we propose as type strain.

Annals of Intensive Care, 2021

Background Dexamethasone decreases mortality in patients with severe coronavirus disease 2019 (CO... more Background Dexamethasone decreases mortality in patients with severe coronavirus disease 2019 (COVID-19) and has become the standard of care during the second wave of pandemic. Dexamethasone is an immunosuppressive treatment potentially increasing the risk of secondary hospital acquired infections in critically ill patients. We conducted an observational retrospective study in three French intensive care units (ICUs) comparing the first and second waves of pandemic to investigate the role of dexamethasone in the occurrence of ventilator-associated pneumonia (VAP) and blood stream infections (BSI). Patients admitted from March to November 2020 with a documented COVID-19 and requiring mechanical ventilation (MV) for ≥ 48 h were included. The main study outcomes were the incidence of VAP and BSI according to the use of dexamethasone. Secondary outcomes were the ventilator-free days (VFD) at day-28 and day-60, ICU and hospital length of stay and mortality. Results Among the 151 patients...

International Journal of Infectious Diseases, 2021

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apol... more This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal

Eurosurveillance, 2011

We report here 14 cases of measles among healthcare workers (HCWs) in Public Hospitals of Marseil... more We report here 14 cases of measles among healthcare workers (HCWs) in Public Hospitals of Marseilles, France that occurred between April and November 2010. All cases but one were under 30 years of age. Following the identification of these cases, we checked the immune status among 154 HCWs who volunteered to take part in the study and showed that 93% and 88% were immune against measles and mumps respectively. HCWs non-immunised against measles were all under 30 years of age.

International Journal of Antimicrobial Agents, 2020

Médecine et Maladies Infectieuses, 2020

Introduction Les infections invasives a pneumocoque representent un probleme de sante publique ma... more Introduction Les infections invasives a pneumocoque representent un probleme de sante publique majeur. De rares epidemies ont ete decrites dans les populations a risques de contact etroit. Nous decrivons ici la survenue de 23 cas de pneumonies documentees a pneumocoque du 19 janvier 2020 au 6 fevrier 2020 chez des travailleurs employes a la renovation d’un bateau de croisiere. Ce chantier naval concerne 3300 travailleurs et 800 membres d’equipages. Materiels et methodes Etude epidemiologique descriptive de cas groupe. Les cas etaient definis par la presence de signes cliniques et radiologiques de pneumonie, et une documentation microbiologique (hemocultures, antigenuries, prelevements respiratoires). Descriptif du controle de l’epidemie : – consultation systematique en service de maladies infectieuses ou aux urgences en cas de signes respiratoires plus ou moins febriles et antibiotherapie probabiliste par amoxicilline systematique ; – vaccination de masse par Pneumovax 23, 1395 vaccinations au sein de cette population. Resultats Au total 23 cas de pneumonies a pneumocoque ont ete diagnostiquees. A l’exception d’un membre d’equipage, tous etaient des travailleurs. Vingt-deux patients ont du etre hospitalises, dont 6 en reanimation. Au 13/02, 2 patients sont encore en reanimation apres une duree de 11 et 17 jours. L’âge moyen des patients est de 42 ans, et dans la moitie des cas ils ne presentaient aucun antecedent (16/31). Dans les autres cas, l’on retrouve des antecedents de chirurgies mineures (4/31) d’asthme (3/31), cardiovasculaires (2/31), d’infections pulmonaires anciennes (2/31) et de prise de traitements anti-inflammatoire au long cours (1/31). Tous les patients qui ont pu etre interroges presentent une exposition soit aux fumes de metallurgies, aux solvants ou aux poussieres. Le masque reglementaire FFP3 bien que porte (13/14), l’est de maniere inconstante dans la majorite des cas (6/8). Neuf de ces pneumonies ont pu etre documentees sur hemocultures. La documentation s’est faite par PCR sur prelevements nasopharynges seule dans 6 cas. Huit souches ont pu etre mises en culture, toutes sensibles a l’amoxicilline, et une presentant une resistance conjointe a l’erythromcycine et a la doxycycline. Au 13/02, 5 souches ont pu etre serotypees : 8 (n2), 4 (n1), 3 (n1), et 9 N (n1), tous inclus dans le Pneumovax 23. Il existe une co-infection virale dans 9 cas (grippe, rhinovirus, VRS, Corona non n-Cov), et bacterienne dans 4 cas (Haemophilus influenza). Conclusion Bien que la prevalence du portage de S. pneumonae soit probablement augmentee du fait de la promiscuite, une contagion interhumaine est peu probable au vu de la multiplicite des serotypes. Notre hypothese principale est que le passage d’un portage commensal a un pathogene responsable pneumonie est declenche par des facteurs environnementaux d’agression des voies aeriennes, notamment l’exposition aux fumes metalliques, poussieres ou solvants, en association avec des co-infections virales. Cette nouvelle serie de cas au sein doit poser la reflexion d’une modification des recommandations vaccinales actuelles.

New Microbes and New Infections, 2020

Journal of Infection and Public Health, 2020

respect to resistant bacteria, one needs to pay attention not only to the proper use of antibioti... more respect to resistant bacteria, one needs to pay attention not only to the proper use of antibiotics but also to the use of disinfectants.