Juliette Caron | Université des Sciences et Technologies de Lille (Lille-1) (original) (raw)

Papers by Juliette Caron

Research paper thumbnail of Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion

Nutrients

The gastrointestinal digestion of food proteins can generate peptides with a wide range of biolog... more The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins were submitted to in vitro SGID. They were then tested in vitro for several bioactivities including measures on: (1) energy homeostasis through their ability to modulate intestinal hormone secretion, to inhibit DPP-IV activity, and to interact with opioid receptors; (2) anti-hypertensive properties through their ability to inhibit ACE activity; (3) anti-inflammatory properties in Caco-2 cells; (4) antioxidant properties through their ability to inhibit production of reactive oxygen species (ROS). Protein intestinal digestions were able to stimulate intestinal hormone secretion by enteroendocrine cells, to inhibit DPP-IV and ACE activities,...

Research paper thumbnail of Explorative screening of potential peptide activities generated by plant-based proteins after in vitro simulation of static digestion

Proceedings of the Nutrition Society

IntroductionThe gastrointestinal digestion of food proteins can generate peptides having a wide r... more IntroductionThe gastrointestinal digestion of food proteins can generate peptides having a wide range of biological activities and possibly involved in many physiological processes. In this study, we screened various potential bioactivities generated by plant-based protein samples as compared to one animal-based protein after in vitro simulation of gastrointestinal digestion (SGID).Materials and methods1 protein samples: Whey protein (WhP) as an animal protein reference (tested twice, in each set), 3 grades of pea protein (PeaP1, PeaP2 and PeaP3) and an hydrolyzed pea protein (HPeaP), pea and wheat albumins (PeaA and WA), wheat protein (WP), potato, fava bean and oat proteins (PP, FBP and OP) were submitted to SGID.Undigested and digested protein samples were then tested in different in vitro, cellular or acellular models, including measures on : 1/energy homeostasis through their ability to promote satiety hormones (CCK and GLP-1) secretion by STC-1 cells, to inhibit DPP-IV activit...

Research paper thumbnail of I dentification and molecular docking of novel ACE inhibitory peptides from protein hydrolysates of shrimp waste

Engineering in Life Sciences

The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive... more The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive activity of shrimp waste proteins was evaluated. The physicochemical characterization, interfacial tension, and surface characteristics of shrimp waste protein hydrolysates (SWPH) using different enzyme/substrate (E/S) (SWPH 5 (SWPH using E/S = 5), SWPH 15 (SWPH using E/S = 15), and SWPH 40 (SWPH using E/S = 40)) were also studied. SWPH 5 , SWPH 15 , and SWPH 40 had an isoelectric pH around 2.07, 2.17, and 2.54 respectively. SWPH 5 exhibited the lowest interfacial tension (68.96 mN/m) followed by SWPH 15 (69.36 mN/m) and SWPH 40 (70.29 mN/m). The in vitro ACE inhibitory activity of shrimp waste protein hydrolysates showed that the most active hydrolysate was obtained using an enzyme/substrate of 15 U/mg (SWPH 15). SWPH 15 had a lower IC 50 value (2.17 mg/mL) than that of SWPH 5 and SWPH 40 (3.65 and 5.7 mg/mL, respectively). This hydrolysate was then purified and characterized. Fraction F1 separated by Sephadex G25 column which presents the best ACE inhibition activity was then separated by reversed-phase high performance liquid chromatography. Four ACE inhibitory peptides were identified and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were SSSKAKKMP, HGEGGRSTHE, WLGHGGRPDHE, and WRMDIDGDIMISE-QEAHQR. The structural modeling of anti-ACE peptides from shrimp waste through docking simulations results showed that these peptides bound to ACE with high affinity.

Research paper thumbnail of Bioactivities of hemorphins released from bovine haemoglobin gastrointestinal digestion: Dual effects on intestinal hormones and DPP-IV regulations

Journal of Functional Foods

Research paper thumbnail of Protein Digestion-Derived Peptides and the Peripheral Regulation of Food Intake

Frontiers in Endocrinology

The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the... more The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the gut-brain axis, and numerous studies have pointed out the significant role played by gut hormones released from enteroendocrine cells. It is well known that digestive products of dietary protein possess a high satiating effect compared to carbohydrates and fat. Nevertheless, the processes occurring in the gut during protein digestion involved in the short-term regulation of food intake are still not totally unraveled. This review provides a concise overview of the current data concerning the implication of food-derived peptides in the peripheral regulation of food intake with a focus on the gut hormones cholecystokinin and glucagon-like peptide 1 regulation and the relationship with some aspects of glucose homeostasis.

Research paper thumbnail of Protein digestion and energy homeostasis: How generated peptides may impact intestinal hormones?

Food Research International, 2015

Research paper thumbnail of Protein Digestion-Derived Peptides and the Peripheral Regulation of Food Intake

Frontiers in Endocrinology, Apr 24, 2017

The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the... more The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the gut-brain axis, and numerous studies have pointed out the significant role played by gut hormones released from enteroendocrine cells. It is well known that digestive products of dietary protein possess a high satiating effect compared to carbohydrates and fat. Nevertheless, the processes occurring in the gut during protein digestion involved in the short-term regulation of food intake are still not totally unraveled. This review provides a concise overview of the current data concerning the implication of food-derived peptides in the peripheral regulation of food intake with a focus on the gut hormones cholecystokinin and glucagon-like peptide 1 regulation and the relationship with some aspects of glucose homeostasis.

Research paper thumbnail of Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion

Food Research International, 2016

Research paper thumbnail of Novel probiotic evidence of lactobacilli on immunomodulation and regulation of satiety hormones release in intestinal cells

Journal of Functional Foods, 2016

Research paper thumbnail of Using Caco-2 cells as novel identification tool for food-derived DPP-IV inhibitors

Food Research International, 2017

Research paper thumbnail of Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion

Food Research International, 2016

Research paper thumbnail of Novel probiotic evidence of lactobacilli on immunomodulation and regulation of satiety hormones release in intestinal cells

Journal of Functional Foods, 2016

Research paper thumbnail of Food peptidomics of in vitro gastrointestinal digestions of partially-purified bovine haemoglobin: low resolution versus high resolution LC-MS/MS analyses

Electrophoresis, Jan 16, 2016

Consumers and governments have become aware how the daily diet may affect the human health. All p... more Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health-promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co-product and, today, the blood proteins remain underexploited despite their bioactive potentiality. Through a comparative food peptidomics approach we illustrate the impact of resolving power, accuracy, sensitivity and acquisition speed of low resolution (LR)- and high resolution (HR)-LC-ESI-MS/MS on the obtained peptide mappings and discuss the limitations of MS-based peptidomics. From in vitro gastrointestinal digestions of partially-purified bovine haemoglobin, we have established the peptide maps of each haemoglobin chain. LR technique (normal bore C18 LC-LR-ESI-MS/MS) allows us to identify without ambiguity 75 unique peptides while the HR ...

Research paper thumbnail of In vitro evidence for gut hormone stimulation release and dipeptidyl-peptidase IV inhibitory activity of protein hydrolysate obtained from cuttlefish (Sepia officinalis) viscera

Food Research International, 2015

Research paper thumbnail of Protein digestion and energy homeostasis: How generated peptides may impact intestinal hormones?

Food Research International, 2015

Research paper thumbnail of Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion

Nutrients

The gastrointestinal digestion of food proteins can generate peptides with a wide range of biolog... more The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins were submitted to in vitro SGID. They were then tested in vitro for several bioactivities including measures on: (1) energy homeostasis through their ability to modulate intestinal hormone secretion, to inhibit DPP-IV activity, and to interact with opioid receptors; (2) anti-hypertensive properties through their ability to inhibit ACE activity; (3) anti-inflammatory properties in Caco-2 cells; (4) antioxidant properties through their ability to inhibit production of reactive oxygen species (ROS). Protein intestinal digestions were able to stimulate intestinal hormone secretion by enteroendocrine cells, to inhibit DPP-IV and ACE activities,...

Research paper thumbnail of Explorative screening of potential peptide activities generated by plant-based proteins after in vitro simulation of static digestion

Proceedings of the Nutrition Society

IntroductionThe gastrointestinal digestion of food proteins can generate peptides having a wide r... more IntroductionThe gastrointestinal digestion of food proteins can generate peptides having a wide range of biological activities and possibly involved in many physiological processes. In this study, we screened various potential bioactivities generated by plant-based protein samples as compared to one animal-based protein after in vitro simulation of gastrointestinal digestion (SGID).Materials and methods1 protein samples: Whey protein (WhP) as an animal protein reference (tested twice, in each set), 3 grades of pea protein (PeaP1, PeaP2 and PeaP3) and an hydrolyzed pea protein (HPeaP), pea and wheat albumins (PeaA and WA), wheat protein (WP), potato, fava bean and oat proteins (PP, FBP and OP) were submitted to SGID.Undigested and digested protein samples were then tested in different in vitro, cellular or acellular models, including measures on : 1/energy homeostasis through their ability to promote satiety hormones (CCK and GLP-1) secretion by STC-1 cells, to inhibit DPP-IV activit...

Research paper thumbnail of I dentification and molecular docking of novel ACE inhibitory peptides from protein hydrolysates of shrimp waste

Engineering in Life Sciences

The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive... more The effect of enzymatic hydrolysis by Savinase on the interfacial properties and antihypertensive activity of shrimp waste proteins was evaluated. The physicochemical characterization, interfacial tension, and surface characteristics of shrimp waste protein hydrolysates (SWPH) using different enzyme/substrate (E/S) (SWPH 5 (SWPH using E/S = 5), SWPH 15 (SWPH using E/S = 15), and SWPH 40 (SWPH using E/S = 40)) were also studied. SWPH 5 , SWPH 15 , and SWPH 40 had an isoelectric pH around 2.07, 2.17, and 2.54 respectively. SWPH 5 exhibited the lowest interfacial tension (68.96 mN/m) followed by SWPH 15 (69.36 mN/m) and SWPH 40 (70.29 mN/m). The in vitro ACE inhibitory activity of shrimp waste protein hydrolysates showed that the most active hydrolysate was obtained using an enzyme/substrate of 15 U/mg (SWPH 15). SWPH 15 had a lower IC 50 value (2.17 mg/mL) than that of SWPH 5 and SWPH 40 (3.65 and 5.7 mg/mL, respectively). This hydrolysate was then purified and characterized. Fraction F1 separated by Sephadex G25 column which presents the best ACE inhibition activity was then separated by reversed-phase high performance liquid chromatography. Four ACE inhibitory peptides were identified and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were SSSKAKKMP, HGEGGRSTHE, WLGHGGRPDHE, and WRMDIDGDIMISE-QEAHQR. The structural modeling of anti-ACE peptides from shrimp waste through docking simulations results showed that these peptides bound to ACE with high affinity.

Research paper thumbnail of Bioactivities of hemorphins released from bovine haemoglobin gastrointestinal digestion: Dual effects on intestinal hormones and DPP-IV regulations

Journal of Functional Foods

Research paper thumbnail of Protein Digestion-Derived Peptides and the Peripheral Regulation of Food Intake

Frontiers in Endocrinology

The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the... more The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the gut-brain axis, and numerous studies have pointed out the significant role played by gut hormones released from enteroendocrine cells. It is well known that digestive products of dietary protein possess a high satiating effect compared to carbohydrates and fat. Nevertheless, the processes occurring in the gut during protein digestion involved in the short-term regulation of food intake are still not totally unraveled. This review provides a concise overview of the current data concerning the implication of food-derived peptides in the peripheral regulation of food intake with a focus on the gut hormones cholecystokinin and glucagon-like peptide 1 regulation and the relationship with some aspects of glucose homeostasis.

Research paper thumbnail of Protein digestion and energy homeostasis: How generated peptides may impact intestinal hormones?

Food Research International, 2015

Research paper thumbnail of Protein Digestion-Derived Peptides and the Peripheral Regulation of Food Intake

Frontiers in Endocrinology, Apr 24, 2017

The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the... more The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the gut-brain axis, and numerous studies have pointed out the significant role played by gut hormones released from enteroendocrine cells. It is well known that digestive products of dietary protein possess a high satiating effect compared to carbohydrates and fat. Nevertheless, the processes occurring in the gut during protein digestion involved in the short-term regulation of food intake are still not totally unraveled. This review provides a concise overview of the current data concerning the implication of food-derived peptides in the peripheral regulation of food intake with a focus on the gut hormones cholecystokinin and glucagon-like peptide 1 regulation and the relationship with some aspects of glucose homeostasis.

Research paper thumbnail of Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion

Food Research International, 2016

Research paper thumbnail of Novel probiotic evidence of lactobacilli on immunomodulation and regulation of satiety hormones release in intestinal cells

Journal of Functional Foods, 2016

Research paper thumbnail of Using Caco-2 cells as novel identification tool for food-derived DPP-IV inhibitors

Food Research International, 2017

Research paper thumbnail of Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion

Food Research International, 2016

Research paper thumbnail of Novel probiotic evidence of lactobacilli on immunomodulation and regulation of satiety hormones release in intestinal cells

Journal of Functional Foods, 2016

Research paper thumbnail of Food peptidomics of in vitro gastrointestinal digestions of partially-purified bovine haemoglobin: low resolution versus high resolution LC-MS/MS analyses

Electrophoresis, Jan 16, 2016

Consumers and governments have become aware how the daily diet may affect the human health. All p... more Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health-promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co-product and, today, the blood proteins remain underexploited despite their bioactive potentiality. Through a comparative food peptidomics approach we illustrate the impact of resolving power, accuracy, sensitivity and acquisition speed of low resolution (LR)- and high resolution (HR)-LC-ESI-MS/MS on the obtained peptide mappings and discuss the limitations of MS-based peptidomics. From in vitro gastrointestinal digestions of partially-purified bovine haemoglobin, we have established the peptide maps of each haemoglobin chain. LR technique (normal bore C18 LC-LR-ESI-MS/MS) allows us to identify without ambiguity 75 unique peptides while the HR ...

Research paper thumbnail of In vitro evidence for gut hormone stimulation release and dipeptidyl-peptidase IV inhibitory activity of protein hydrolysate obtained from cuttlefish (Sepia officinalis) viscera

Food Research International, 2015

Research paper thumbnail of Protein digestion and energy homeostasis: How generated peptides may impact intestinal hormones?

Food Research International, 2015