Sebastien HUPONT | Université de Lorraine (original) (raw)

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Papers by Sebastien HUPONT

Research paper thumbnail of Insights into the electrocatalytic behavior of nitrogen and sulfur co-doped carbon nanotubes toward oxygen reduction reaction in alkaline media

Journal of Materials Science, Sep 1, 2022

Research paper thumbnail of Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic differentiation

Stem Cell Research & Therapy, Jul 5, 2017

Research paper thumbnail of Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells

Bio-medical Materials and Engineering, Mar 30, 2017

Research paper thumbnail of Effect of nicotine on the proliferation and chondrogenic differentiation of the human Wharton’s jelly mesenchymal stem cells

Bio-medical Materials and Engineering, Mar 30, 2017

Research paper thumbnail of Photodynamic therapy with conventional and PEGylated liposomal formulations of mTHPC (temoporfin): comparison of treatment efficacy and distribution characteristics in vivo

International Journal of Nanomedicine, Oct 1, 2013

Research paper thumbnail of Erratum to: Pro-apoptotic effect of Δ2-TGZ in “claudin-1-low” triple-negative breast cancer cells: involvement of claudin-1

Breast Cancer Research and Treatment, Jul 31, 2017

Research paper thumbnail of Insights into Solid-To-Solid Transformation of MOF Amorphous Phases

Inorganic Chemistry, Aug 24, 2022

Research paper thumbnail of Insights into Solid-To-Solid Transformation of MOF Amorphous Phases

Research paper thumbnail of Expression of HSP70 by oxidative stress contributes to the inhibitory effect of 15d-PGJ2 on inducible prostaglandin pathway in chondrocyte

HAL (Le Centre pour la Communication Scientifique Directe), Apr 1, 2015

Research paper thumbnail of Innovative and non-invasive evaluation of the quality of collagen scaffold functionalized by human mesenchymal stem cells before graft in cartilage lesion = Évaluation innovante et non invasive de la qualité d’un biomatériau à base de collagène fonctionnalisé par des cellules souches mésenchymate...

HAL (Le Centre pour la Communication Scientifique Directe), Dec 4, 2014

Research paper thumbnail of Real‐time Raman spectroscopy measurements to monitor the effect of polypropylene crosslinking on its true mechanical behaviour

Journal of Raman Spectroscopy, 2021

In‐situ Raman spectroscopy analysis to mechanical tests were performed to investigate the deforma... more In‐situ Raman spectroscopy analysis to mechanical tests were performed to investigate the deformation micromechanisms of an isotactic polypropylene‐grafted maleic anhydride (PP‐g‐MAH) depending on the crosslinking degree provided by functional polyether amine molecules. The switch from brittle to highly ductile mechanical behaviours was focused in terms of microstructure modifications in real time during stretching. From the Raman spectral analysis, crystallinity index and macromolecular chains orientation factors for both crystalline and amorphous phases were determined. A new orientation factor relative to the polyether segments was proposed. The results shown allow a more complete understanding of the proper micromechanisms of deformation involved in each phase and their interaction between one another. These results also highlight the impact of the crosslinking reaction on the material resilience improvement.

Research paper thumbnail of Digital Analysis of Phase Image to Study Mesenchymal Stem Cell Culture

Research paper thumbnail of Resilience improvement of an isotactic polypropylene-g-maleic anhydride by crosslinking using polyether triamine agents

Polymer, 2019

Abstract The elaboration of new hybrid polymer/metal composite materials depends on the developme... more Abstract The elaboration of new hybrid polymer/metal composite materials depends on the development of innovative polymer matrices with improved mechanical properties and high effective adhesion behaviour with metal surfaces. This way, a new type of polyolefin was developed based on a peculiar crosslinking reaction. For this elaboration, an iPP-g-MAH with 1 wt% of MAH was crosslinked with polyether triamine molecules by twin screw reactive extrusion. The evolution of the crosslinking reaction, the microstructure and the mechanical behaviour of the different materials were studied as a function of the NH2:MAH molar ratio in the range of 0:1 and 1.5:1. Gel content determination and FTIR analysis were performed to characterise the material at the molecular scale, when differential scanning calorimetry, wide angle X-rays scattering and self-successive auto-nucleation techniques were used for a deep microstructure analysis. Brillouin spectroscopy showed an interesting evolution of the elastic constants at the microscopic scale and results were in good agreement with those obtained at the macroscopic scale by a video-controlled uniaxial tensile test. Moreover, it was demonstrated that the true mechanical behaviours switch from brittle to ductile illustrating an important improvement of the resilience ability of the synthetized materials as a function of the NH2:MAH molar ratio even for molar ratio other than an equimolar ratio, with remaining MAH or NH2 chemical groups for further chemical reaction.

Research paper thumbnail of Pro-apoptotic effect of Δ2-TGZ in "claudin-1-low" triple-negative breast cancer cells: involvement of claudin-1

Breast cancer research and treatment, Jan 5, 2017

40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tigh... more 40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tight junction. Patients with these "claudin-1-low" tumors present a higher relapse incidence. A major challenge in oncology is the development of innovative therapies for such poor prognosis tumors. In this context, we study the anticancer effects of ∆2-TGZ, a compound derived from troglitazone (TGZ), on cell models of these tumors. In MDA-MB-231 and Hs578T "claudin-1-low" TNBC cells, Δ2-TGZ treatment induced claudin-1 protein expression and triggered apoptosis as measured by FACS analysis (annexin V/PI co-staining). Interestingly, in the non-tumorigenic human breast epithelial cell line MCF-10A, the basal level of claudin-1 was not modified following Δ2-TGZ treatment, which did not induce apoptosis. Furthermore, claudin-1-transfected MDA-MB-231 and Hs578T cells displayed a significant increase of cleaved PARP-1 and caspase 7, caspase 3/7 activities, and TUNEL staining. ...

Research paper thumbnail of Erratum to: Pro-apoptotic effect of Δ2-TGZ in "claudin-1-low" triple-negative breast cancer cells: involvement of claudin-1

Breast cancer research and treatment, Jan 31, 2017

Research paper thumbnail of Effect of nicotine on the proliferation and chondrogenic differentiation of the human Wharton's jelly mesenchymal stem cells

Bio-medical materials and engineering, 2017

Osteoarthritis (OA) is a chronic joint disease characterized by a progressive and irreversible de... more Osteoarthritis (OA) is a chronic joint disease characterized by a progressive and irreversible degeneration of articular cartilage. Among the environmental risk factors of OA, tobacco consumption features prominently, although, there is a great controversy regarding the role of tobacco smoking in OA development. Among the numerous chemicals present in cigarette smoke, nicotine is one of the most physiologically active molecules. The aim of the study was (i) to measure the impact of nicotine on the proliferation and chondrogenic differentiation of mesenchymal stem cells from the human Wharton's jelly (hWJ-MSCs) into chondrocytes, (ii) to investigate whether the α7 nicotinic acetylcholine receptors (nAChRs) was expressed in hWJ-MSCs and could play a role in the process. The project benefits from the availability of an umbilical cord bank from which hWJ-MSCs were originated. The hWJ-MSCs were cultured and used up to passage 5. The proliferation of hWJ-MSCs with 5 μM nicotine was me...

Research paper thumbnail of New tools for non-invasive exploration of collagen network in cartilaginous tissue-engineered substitute

Bio-medical materials and engineering, 2017

In tissue engineering approaches, the quality of substitutes is a key element to determine its ab... more In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engin...

Research paper thumbnail of Nacre extract restores the mineralization capacity of subchondral osteoarthritis osteoblasts

Journal of Structural Biology, 2015

Research paper thumbnail of Changes in humic acid conformation during coagulation with ferric chloride: Implications for drinking water treatment

Research paper thumbnail of SHG as a new modality for large field of view imaging to monitor tissue collagen network

Bio-medical materials and engineering, 2012

For this study, we have considered a new large field of view imaging dedicated to matrix collagen... more For this study, we have considered a new large field of view imaging dedicated to matrix collagen (no stained samples). To integrate a multidimensional scale (non-sliced samples), a femtosecond oscillator (two photon excitation laser) has been coupled with a large field optical setup to collect SHG signal. We introduced an index (F-SHG) based on decay time response measured by TCSPC for, respectively, Fluorescence (F) and Second Harmonic Generation (SHG) values. For samples where protein collagen is the major component of extracellular matrix (skin) or not (nacre), we compared the index distribution (from 2 to 12) obtained with large field optical setup. In this work, we showed for the first time that multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) could be an innovative and non invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.

Research paper thumbnail of Insights into the electrocatalytic behavior of nitrogen and sulfur co-doped carbon nanotubes toward oxygen reduction reaction in alkaline media

Journal of Materials Science, Sep 1, 2022

Research paper thumbnail of Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic differentiation

Stem Cell Research & Therapy, Jul 5, 2017

Research paper thumbnail of Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells

Bio-medical Materials and Engineering, Mar 30, 2017

Research paper thumbnail of Effect of nicotine on the proliferation and chondrogenic differentiation of the human Wharton’s jelly mesenchymal stem cells

Bio-medical Materials and Engineering, Mar 30, 2017

Research paper thumbnail of Photodynamic therapy with conventional and PEGylated liposomal formulations of mTHPC (temoporfin): comparison of treatment efficacy and distribution characteristics in vivo

International Journal of Nanomedicine, Oct 1, 2013

Research paper thumbnail of Erratum to: Pro-apoptotic effect of Δ2-TGZ in “claudin-1-low” triple-negative breast cancer cells: involvement of claudin-1

Breast Cancer Research and Treatment, Jul 31, 2017

Research paper thumbnail of Insights into Solid-To-Solid Transformation of MOF Amorphous Phases

Inorganic Chemistry, Aug 24, 2022

Research paper thumbnail of Insights into Solid-To-Solid Transformation of MOF Amorphous Phases

Research paper thumbnail of Expression of HSP70 by oxidative stress contributes to the inhibitory effect of 15d-PGJ2 on inducible prostaglandin pathway in chondrocyte

HAL (Le Centre pour la Communication Scientifique Directe), Apr 1, 2015

Research paper thumbnail of Innovative and non-invasive evaluation of the quality of collagen scaffold functionalized by human mesenchymal stem cells before graft in cartilage lesion = Évaluation innovante et non invasive de la qualité d’un biomatériau à base de collagène fonctionnalisé par des cellules souches mésenchymate...

HAL (Le Centre pour la Communication Scientifique Directe), Dec 4, 2014

Research paper thumbnail of Real‐time Raman spectroscopy measurements to monitor the effect of polypropylene crosslinking on its true mechanical behaviour

Journal of Raman Spectroscopy, 2021

In‐situ Raman spectroscopy analysis to mechanical tests were performed to investigate the deforma... more In‐situ Raman spectroscopy analysis to mechanical tests were performed to investigate the deformation micromechanisms of an isotactic polypropylene‐grafted maleic anhydride (PP‐g‐MAH) depending on the crosslinking degree provided by functional polyether amine molecules. The switch from brittle to highly ductile mechanical behaviours was focused in terms of microstructure modifications in real time during stretching. From the Raman spectral analysis, crystallinity index and macromolecular chains orientation factors for both crystalline and amorphous phases were determined. A new orientation factor relative to the polyether segments was proposed. The results shown allow a more complete understanding of the proper micromechanisms of deformation involved in each phase and their interaction between one another. These results also highlight the impact of the crosslinking reaction on the material resilience improvement.

Research paper thumbnail of Digital Analysis of Phase Image to Study Mesenchymal Stem Cell Culture

Research paper thumbnail of Resilience improvement of an isotactic polypropylene-g-maleic anhydride by crosslinking using polyether triamine agents

Polymer, 2019

Abstract The elaboration of new hybrid polymer/metal composite materials depends on the developme... more Abstract The elaboration of new hybrid polymer/metal composite materials depends on the development of innovative polymer matrices with improved mechanical properties and high effective adhesion behaviour with metal surfaces. This way, a new type of polyolefin was developed based on a peculiar crosslinking reaction. For this elaboration, an iPP-g-MAH with 1 wt% of MAH was crosslinked with polyether triamine molecules by twin screw reactive extrusion. The evolution of the crosslinking reaction, the microstructure and the mechanical behaviour of the different materials were studied as a function of the NH2:MAH molar ratio in the range of 0:1 and 1.5:1. Gel content determination and FTIR analysis were performed to characterise the material at the molecular scale, when differential scanning calorimetry, wide angle X-rays scattering and self-successive auto-nucleation techniques were used for a deep microstructure analysis. Brillouin spectroscopy showed an interesting evolution of the elastic constants at the microscopic scale and results were in good agreement with those obtained at the macroscopic scale by a video-controlled uniaxial tensile test. Moreover, it was demonstrated that the true mechanical behaviours switch from brittle to ductile illustrating an important improvement of the resilience ability of the synthetized materials as a function of the NH2:MAH molar ratio even for molar ratio other than an equimolar ratio, with remaining MAH or NH2 chemical groups for further chemical reaction.

Research paper thumbnail of Pro-apoptotic effect of Δ2-TGZ in "claudin-1-low" triple-negative breast cancer cells: involvement of claudin-1

Breast cancer research and treatment, Jan 5, 2017

40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tigh... more 40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tight junction. Patients with these "claudin-1-low" tumors present a higher relapse incidence. A major challenge in oncology is the development of innovative therapies for such poor prognosis tumors. In this context, we study the anticancer effects of ∆2-TGZ, a compound derived from troglitazone (TGZ), on cell models of these tumors. In MDA-MB-231 and Hs578T "claudin-1-low" TNBC cells, Δ2-TGZ treatment induced claudin-1 protein expression and triggered apoptosis as measured by FACS analysis (annexin V/PI co-staining). Interestingly, in the non-tumorigenic human breast epithelial cell line MCF-10A, the basal level of claudin-1 was not modified following Δ2-TGZ treatment, which did not induce apoptosis. Furthermore, claudin-1-transfected MDA-MB-231 and Hs578T cells displayed a significant increase of cleaved PARP-1 and caspase 7, caspase 3/7 activities, and TUNEL staining. ...

Research paper thumbnail of Erratum to: Pro-apoptotic effect of Δ2-TGZ in "claudin-1-low" triple-negative breast cancer cells: involvement of claudin-1

Breast cancer research and treatment, Jan 31, 2017

Research paper thumbnail of Effect of nicotine on the proliferation and chondrogenic differentiation of the human Wharton's jelly mesenchymal stem cells

Bio-medical materials and engineering, 2017

Osteoarthritis (OA) is a chronic joint disease characterized by a progressive and irreversible de... more Osteoarthritis (OA) is a chronic joint disease characterized by a progressive and irreversible degeneration of articular cartilage. Among the environmental risk factors of OA, tobacco consumption features prominently, although, there is a great controversy regarding the role of tobacco smoking in OA development. Among the numerous chemicals present in cigarette smoke, nicotine is one of the most physiologically active molecules. The aim of the study was (i) to measure the impact of nicotine on the proliferation and chondrogenic differentiation of mesenchymal stem cells from the human Wharton's jelly (hWJ-MSCs) into chondrocytes, (ii) to investigate whether the α7 nicotinic acetylcholine receptors (nAChRs) was expressed in hWJ-MSCs and could play a role in the process. The project benefits from the availability of an umbilical cord bank from which hWJ-MSCs were originated. The hWJ-MSCs were cultured and used up to passage 5. The proliferation of hWJ-MSCs with 5 μM nicotine was me...

Research paper thumbnail of New tools for non-invasive exploration of collagen network in cartilaginous tissue-engineered substitute

Bio-medical materials and engineering, 2017

In tissue engineering approaches, the quality of substitutes is a key element to determine its ab... more In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engin...

Research paper thumbnail of Nacre extract restores the mineralization capacity of subchondral osteoarthritis osteoblasts

Journal of Structural Biology, 2015

Research paper thumbnail of Changes in humic acid conformation during coagulation with ferric chloride: Implications for drinking water treatment

Research paper thumbnail of SHG as a new modality for large field of view imaging to monitor tissue collagen network

Bio-medical materials and engineering, 2012

For this study, we have considered a new large field of view imaging dedicated to matrix collagen... more For this study, we have considered a new large field of view imaging dedicated to matrix collagen (no stained samples). To integrate a multidimensional scale (non-sliced samples), a femtosecond oscillator (two photon excitation laser) has been coupled with a large field optical setup to collect SHG signal. We introduced an index (F-SHG) based on decay time response measured by TCSPC for, respectively, Fluorescence (F) and Second Harmonic Generation (SHG) values. For samples where protein collagen is the major component of extracellular matrix (skin) or not (nacre), we compared the index distribution (from 2 to 12) obtained with large field optical setup. In this work, we showed for the first time that multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) could be an innovative and non invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.