An Es | Djillali Liabes University (original) (raw)
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Papers by An Es
Journal of Business Venturing, 1998
Biochimica Et Biophysica Acta-reviews on Cancer, 2003
Evolutionary Computation, 2003
Environmental Science & Technology, 1979
One objective of this article is to relate the historical origins of EPA's Priority ... more One objective of this article is to relate the historical origins of EPA's Priority Pollutants and the develop-ment of the Priority Pollutant Pro-tocol. Most people are unaware of the difficulties involved in providing methods for the necessary analytical support for these pollutants. A ...
... Copyright © 2011 Elsevier BV All rights reserved. SciVerse® is a registered trademark of Else... more ... Copyright © 2011 Elsevier BV All rights reserved. SciVerse® is a registered trademark of Elsevier Properties SA, used under license. ScienceDirect® is a registered trademark of Elsevier BV.
Arthritis and Rheumatism, 1994
Mechanisms of Development, 1998
Green fluorescent protein (GFP) and its variants currently represent the only non-invasive marker... more Green fluorescent protein (GFP) and its variants currently represent the only non-invasive markers available for labeling mammalian cells in culture or in a multicellular organism through transgenesis. To date this marker gene has been widely used in the study of many organisms, but as yet has not found large-scale application in mammals due to problems encountered with weak fluorescence and instability of the wild-type protein at higher temperatures. Recently, though, several mutants have been made in the wild-type (wt) GFP so as to improve its thermostability and fluorescence. EGFP (enhanced GFP) is one such wtGFP variant. As a first step in assessing the use of EGFP in ES cell-mediated strategies, we have established a mouse embryonic stem (ES) cell lines expressing EGFP, which can be propagated in culture, reintroduced into mice. or induced to differentiate in vitro, while still maintaining ubiquitous EGFP expression. From the results presented we can suggest that: 1) possible improvements in the efficiency of transgenic regimes requiring the germline transmission of ES cells by aggregation chimeras can be made by the preselection chimeric embryos at the blastocyst stage: (2) the expression of a noninvasive marker, driven by a promoter that is active during early postimplantation development, allows access to embryos during a window of embryonic development that has previously been difficult to investigate (3) the behavior of mutant ES cells can be followed with simple microscopic observation of chimeric embryos or adult animals comprising green fluorescent cells/tissues. and (4) intercrosses of F1 mice and subsequent generations of animals show that progeny can be genotyped by UV light, such that mice homozygous for the transgene can be distinguished from hemizygotes due to their increased fluorescence.
Journal of Business Venturing, 1998
Biochimica Et Biophysica Acta-reviews on Cancer, 2003
Evolutionary Computation, 2003
Environmental Science & Technology, 1979
One objective of this article is to relate the historical origins of EPA's Priority ... more One objective of this article is to relate the historical origins of EPA's Priority Pollutants and the develop-ment of the Priority Pollutant Pro-tocol. Most people are unaware of the difficulties involved in providing methods for the necessary analytical support for these pollutants. A ...
... Copyright © 2011 Elsevier BV All rights reserved. SciVerse® is a registered trademark of Else... more ... Copyright © 2011 Elsevier BV All rights reserved. SciVerse® is a registered trademark of Elsevier Properties SA, used under license. ScienceDirect® is a registered trademark of Elsevier BV.
Arthritis and Rheumatism, 1994
Mechanisms of Development, 1998
Green fluorescent protein (GFP) and its variants currently represent the only non-invasive marker... more Green fluorescent protein (GFP) and its variants currently represent the only non-invasive markers available for labeling mammalian cells in culture or in a multicellular organism through transgenesis. To date this marker gene has been widely used in the study of many organisms, but as yet has not found large-scale application in mammals due to problems encountered with weak fluorescence and instability of the wild-type protein at higher temperatures. Recently, though, several mutants have been made in the wild-type (wt) GFP so as to improve its thermostability and fluorescence. EGFP (enhanced GFP) is one such wtGFP variant. As a first step in assessing the use of EGFP in ES cell-mediated strategies, we have established a mouse embryonic stem (ES) cell lines expressing EGFP, which can be propagated in culture, reintroduced into mice. or induced to differentiate in vitro, while still maintaining ubiquitous EGFP expression. From the results presented we can suggest that: 1) possible improvements in the efficiency of transgenic regimes requiring the germline transmission of ES cells by aggregation chimeras can be made by the preselection chimeric embryos at the blastocyst stage: (2) the expression of a noninvasive marker, driven by a promoter that is active during early postimplantation development, allows access to embryos during a window of embryonic development that has previously been difficult to investigate (3) the behavior of mutant ES cells can be followed with simple microscopic observation of chimeric embryos or adult animals comprising green fluorescent cells/tissues. and (4) intercrosses of F1 mice and subsequent generations of animals show that progeny can be genotyped by UV light, such that mice homozygous for the transgene can be distinguished from hemizygotes due to their increased fluorescence.