Alfonso Forgione | Università Degli Studi Dell'Aquila (original) (raw)
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Papers by Alfonso Forgione
Genes
The evaluation of the integrity and quantity of DNA extracted from archaeological human remains i... more The evaluation of the integrity and quantity of DNA extracted from archaeological human remains is a fundamental step before using the latest generation sequencing techniques in the study of evolutionary processes. Ancient DNA is highly fragmented and chemically modified; therefore, the present study aims to identify indices that can allow the identification of potentially amplifiable and sequenceable DNA samples, reducing failures and research costs. Ancient DNA was extracted from five human bone remains from the archaeological site of Amiternum L’Aquila, Italy dating back to the 9th–12th century and was compared with standard DNA fragmented by sonication. Given the different degradation kinetics of mitochondrial DNA compared to nuclear DNA, the mitochondrially encoded 12s RNA and 18s ribosomal RNA genes were taken into consideration; fragments of various sizes were amplified in qPCR and the size distribution was thoroughly investigated. DNA damage degree was evaluated by calculati...
I castelli di Ocre e di Grimbosq : dalla regione del Calvados in Normandia alla vallata amiternino-forconese in Abruzzo, due identiche esperienze di incastellamento normanno a confronto, 2016
Amiternum (AQ) : Campo S. Maria, campagna di scavo 2014, 2015
Amiternum (AQ), Campo Santa Maria : dinamiche insediative e fasi sepolcrali di un nuovo polo di potere : nova sintesi delle ricerche in corso, 2019
Archeologia delle campane nell'Abruzzo interno : verso la costruzione di un modello produttivo e il riconoscimento di maestranze specializzate in un'area dell'Appennino abruzzese, 2017
PLOS ONE
Molecular biology techniques are increasingly being used in sex identification of skeletal remain... more Molecular biology techniques are increasingly being used in sex identification of skeletal remains when traditional anthropometric analyzes are not successful in identifying sex of remains that are incomplete, fragmented and /or of immature individuals. In the present work, we investigated the possibility of determining sex by using the qPCR-duplex method for both ancient and modern DNA samples. This method involves the co-amplification of two genes in a single reaction system and the subsequent analysis of the fusion curves; the gene sequences used for the construction of suitable primers are those of steroid sulfatase (STS) and testis specific protein Y-linked 1 (TSPY) genes which turned out to be two sensitive markers as they have a detection limit of 60 pg and 20 pg respectively on modern DNA. The validity of the method was verified on modern DNA in which gender was identified in all the samples with 100% accuracy; thus, allowing for the same results as the classic method with a...
FOLD&R Fasti On Line Documents & Research, 502, 2021
During the 2019 excavation campaign, in the archaeological site of "Campo Santa Maria" in Amitern... more During the 2019 excavation campaign, in the archaeological site of "Campo Santa Maria" in Amiternum, in some rooms belonging to the imperial age plant, originally intended for domestic and service roles (Building F, Period III), consistent traces of metallurgical and metal activities have been found, ascibable to the reconversions of use of the spaces, that took place during the 5th-6th century. During the construction of baptistery in the episcopal complex, the area in front was used for the realization of some forges, useful to recycling metal belonging to the factories, that persist in the area. The site, therefore, is part of the very widespread hystorical cases in late ancient Italy, attribuitable to the reuse and systematic dispossession of public or private buildings, indicative of a process of deconstruction of the ancient city. The site of Campo S. Maria in Amiternum, un-like other areas of the peninsula, offers a different overview because the area, affected by the aforementioned expolations, was at the same time the scene of new building events which, shortly thereafter, would have constituted a new centripetal pole for the city being redefined.
Censimento e cronotipologia degli Elementi Architettonici della città dell’Aquila: dati prelimi... more Censimento e cronotipologia degli Elementi Architettonici della città dell’Aquila: dati preliminari per la realizzazione di un Sistema Informativo del tessuto edilizio medievale e delle architetture storiche
Genes
The evaluation of the integrity and quantity of DNA extracted from archaeological human remains i... more The evaluation of the integrity and quantity of DNA extracted from archaeological human remains is a fundamental step before using the latest generation sequencing techniques in the study of evolutionary processes. Ancient DNA is highly fragmented and chemically modified; therefore, the present study aims to identify indices that can allow the identification of potentially amplifiable and sequenceable DNA samples, reducing failures and research costs. Ancient DNA was extracted from five human bone remains from the archaeological site of Amiternum L’Aquila, Italy dating back to the 9th–12th century and was compared with standard DNA fragmented by sonication. Given the different degradation kinetics of mitochondrial DNA compared to nuclear DNA, the mitochondrially encoded 12s RNA and 18s ribosomal RNA genes were taken into consideration; fragments of various sizes were amplified in qPCR and the size distribution was thoroughly investigated. DNA damage degree was evaluated by calculati...
I castelli di Ocre e di Grimbosq : dalla regione del Calvados in Normandia alla vallata amiternino-forconese in Abruzzo, due identiche esperienze di incastellamento normanno a confronto, 2016
Amiternum (AQ) : Campo S. Maria, campagna di scavo 2014, 2015
Amiternum (AQ), Campo Santa Maria : dinamiche insediative e fasi sepolcrali di un nuovo polo di potere : nova sintesi delle ricerche in corso, 2019
Archeologia delle campane nell'Abruzzo interno : verso la costruzione di un modello produttivo e il riconoscimento di maestranze specializzate in un'area dell'Appennino abruzzese, 2017
PLOS ONE
Molecular biology techniques are increasingly being used in sex identification of skeletal remain... more Molecular biology techniques are increasingly being used in sex identification of skeletal remains when traditional anthropometric analyzes are not successful in identifying sex of remains that are incomplete, fragmented and /or of immature individuals. In the present work, we investigated the possibility of determining sex by using the qPCR-duplex method for both ancient and modern DNA samples. This method involves the co-amplification of two genes in a single reaction system and the subsequent analysis of the fusion curves; the gene sequences used for the construction of suitable primers are those of steroid sulfatase (STS) and testis specific protein Y-linked 1 (TSPY) genes which turned out to be two sensitive markers as they have a detection limit of 60 pg and 20 pg respectively on modern DNA. The validity of the method was verified on modern DNA in which gender was identified in all the samples with 100% accuracy; thus, allowing for the same results as the classic method with a...
FOLD&R Fasti On Line Documents & Research, 502, 2021
During the 2019 excavation campaign, in the archaeological site of "Campo Santa Maria" in Amitern... more During the 2019 excavation campaign, in the archaeological site of "Campo Santa Maria" in Amiternum, in some rooms belonging to the imperial age plant, originally intended for domestic and service roles (Building F, Period III), consistent traces of metallurgical and metal activities have been found, ascibable to the reconversions of use of the spaces, that took place during the 5th-6th century. During the construction of baptistery in the episcopal complex, the area in front was used for the realization of some forges, useful to recycling metal belonging to the factories, that persist in the area. The site, therefore, is part of the very widespread hystorical cases in late ancient Italy, attribuitable to the reuse and systematic dispossession of public or private buildings, indicative of a process of deconstruction of the ancient city. The site of Campo S. Maria in Amiternum, un-like other areas of the peninsula, offers a different overview because the area, affected by the aforementioned expolations, was at the same time the scene of new building events which, shortly thereafter, would have constituted a new centripetal pole for the city being redefined.
Censimento e cronotipologia degli Elementi Architettonici della città dell’Aquila: dati prelimi... more Censimento e cronotipologia degli Elementi Architettonici della città dell’Aquila: dati preliminari per la realizzazione di un Sistema Informativo del tessuto edilizio medievale e delle architetture storiche