Regina Breganó | Universidade Estadual de Londrina (original) (raw)
Papers by Regina Breganó
to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and conge... more to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and congenital toxoplasmosis in a Sentinel Unit (SU) and describe reported cases. this was a descriptive study of the implantation of a notification protocol using a specific notification form for suspected cases of toxoplasmosis in pregnant women and congenital toxoplasmosis in a reference center in Paraná State, Brazil, from August 2013 to August 2014. all 64 suspected cases were notified and case investigation was completed by the SU Epidemiology Sector; 63 received prenatal care and 51 received treatment during pregnancy; 7 of the children being clinically monitored had confirmed diagnosis of congenital toxoplasmosis. implanting toxoplasmosis notification afforded epidemiological, clinical and diagnostic data on the disease that contributed to the assessment of the clinical evolution of children exposed to Toxoplasma gondii.
Brazilian Journal of Infectious Diseases, Sep 1, 2014
Malaria is endemic in the North of Brazil. However, Hyperreactive Malarious Splenomegaly (HMS) ha... more Malaria is endemic in the North of Brazil. However, Hyperreactive Malarious Splenomegaly (HMS) has been rarely described. Splenomegaly in HIV/Aids infection has a large differential diagnosis, but malaria is a cause of gross splenomegaly, regardless of the HIV status. In this paper, we report the case of a 50-year-old man, HIV positive, with massive splenomegaly and multiple malaria infections in the past. He fulfilled the criteria for HMS, received a short course of anti-malarial treatment and weekly quimioprofilatic Chloroquine. In 9 months, he had great clinical and laboratorial improvement confirming the HMS, a rare diagnosis in Brazil.
Revista de Ciência Veterinária e Saúde Pública, Oct 6, 2016
International journal of epidemiologic research, 2017
Background and aims: We investigated the children of Parana State, Brazil the prevalence of intes... more Background and aims: We investigated the children of Parana State, Brazil the prevalence of intestinal parasitosis and the associated factors involved in the transmission of intestinal parasites, and we genotyped the Giardia duodenalis isolates obtained. Methods: Fecal samples were analyzed by established microscopic methods. G. duodenalis positive samples were subjected to genotypic characterization by PCR amplification of sequences of the glutamate dehydrogenase gene (gdh) and by enzymatic digestion with the restriction enzyme NlaIV for classification of genotypes. Results: Of the 877 samples tested, 41% were positive for some intestinal parasitosis, the most common being the presence of protozoa (87.8%). Lack of basic sanitation and poor health education were associated for the intestinal parasite cases found, and the only associated factor for giardiasis was low family income. The G. duodenalis assemblages of gdh amplified samples were 68.6% B and 31.4% AII. Conclusion: These da...
Semina: Ciências Agrárias, 2017
The objective of this study was to investigate the presence of anti-Leishmania spp. antibodies us... more The objective of this study was to investigate the presence of anti-Leishmania spp. antibodies using an indirect immunofluorescence technique in the serum of equidae slaughtered in two slaughterhouses registered with the Federal Inspection Service. Titers greater than 40 were considered reagents. Blood samples were collected from 398 animals of both sexes, with different ages, and coming from six Brazilian states, of which 46.0% (183/398) were reactive, indicating that these animals were exposed to the leishmaniasis agent that is widely distributed in Brazil.
Sao Paulo Medical Journal, 2020
The transmission routes for toxoplasmosis include consumption of raw foods, undercooked meats and... more The transmission routes for toxoplasmosis include consumption of raw foods, undercooked meats and untreated water; contact with soil contaminated with the evolutionary infective forms of the protozoon Toxoplasma gondii (T. gondii); and transplacental infection. 1 Differences in pregnant women's food intake and cultural habits around the world are responsible for variations in the prevalence of congenital toxoplasmosis among countries, 2 and between communities within the same nation. 3 Preventive measures are common to all populations, since they concentrate on health education and early detection of infection during pregnancy. 4 However, the public health strategies for prevention of this parasitosis need to be appropriate for each situation. T. gondii infection is a major problem within gestational health because of the possibility of irreversibly damaging the fetus. 1 The main types of damage reported have included chorioretinitis, deafness, intracranial calcification, microcephaly, hydrocephalus, seizures and intellectual disability. In addition, occurrences of spontaneous abortions, neonatal death and neurodevelopmental disorders have been recorded. 5 Researchers from the World Health Organization (WHO) reported that in 2010, despite neglect due to little attention given to the prevalence, prevention and treatment of toxoplasmosis, 10.3 million cases of this disease were recorded worldwide, with 825,000 Disability-Adjusted Life Years (DALYs), one of the highest among foodborne parasitic diseases. 2 The Brazilian strains of T. gondii are highly damaging to the eye tissue, in comparison with strains in other countries. 6 However, reduction of the incidence of congenital infection, through early identification of maternal infection and adoption of strategic therapy for pregnant women and
Preventive Veterinary Medicine, 2020
Transboundary and Emerging Diseases, 2020
Toxoplasmosis and neosporosis are diseases caused by the protozoa Toxoplasma gondii and Neospora ... more Toxoplasmosis and neosporosis are diseases caused by the protozoa Toxoplasma gondii and Neospora caninum, respectively. They may be associated with reproductive and economic losses due to infection, resulting from inadequate management practices in ovine herds. Toxoplasma gondii is one of the major parasitic aetiological agents involved in abortion of sheep and goats in the USA and Scotland (Dubey & Kirkbride 1984). Additionally, Larsson, Jamra, Guimarães, Pattoli, and Silva (1980) have verified the presence of antibodies against T. gondii in sheep herds from the city of Uruguaiana, Rio Grande do Sul (RS), Brazil. Moreover, we have previously (Romanelli et al., 2007) investigated the occurrence of N. caninum in sheep from the state of Paraná, where a prevalence of 9.5% (29/305) was reported, but risk factors associated with seropositivity were not identified. Pinto et al., (2012) demonstrated N. caninum as the primary
Medicina Veterinária (UFRPE), 2017
Toxoplasmosis is a zoonosis caused by the Toxoplasma gondii protozoa. The congenital form happens... more Toxoplasmosis is a zoonosis caused by the Toxoplasma gondii protozoa. The congenital form happens when the parasite reaches the fetus through the placenta and causes damages of different intensities, which can be consequence of the strain virulence, of the immune response capability of the pregnant woman or even of the pregnancy stage, and can result in fetal death or severe clinical symptomatology. The aim of this paper was to determine the prevalence of antibodies to Toxoplasma gondii antibodies in pregnant woman seen at the Basic Health Units (BHU) in the city of Umuarama (PR) and check for possible toxoplasmosis associations with social-demographic characteristics, behavior and environmental variables. In this study, a total of 813 pregnant women who had their prenatal exams at the BHU in the city of Umuarama (PR) were included. Blood samples collected from these subjects were submitted to IgG and IgM anti-T. gondii antibody survey. Each pregnant woman answered an epidemiologica...
Zoonoses and Public Health, 2019
Background: The objective of this study was to report an outbreak of human toxoplasmosis occurrin... more Background: The objective of this study was to report an outbreak of human toxoplasmosis occurring in the municipality of Montes Claros de Goiás, Goiás, Brazil, from December 2015 to August 2016. Seven acute cases in June 2016 triggered the subsequent search. Methods: A total of 251 individuals were selected through an active search, of which 114 (45.4%) agreed to participate in the research and blood collection. For serological diagnosis were used enzyme-linked immunosorbent assay (ELISA) for IgG and IgM and avidity tests. Results: Of the 114 serum samples evaluated, 12.3% (14/114) showed antibodies against Toxoplasma gondii, with a profile indicative of acute infection. Samples of artisan fresh cheese, public water, vegetables and irrigation water were collected. Toxoplasma gondii DNA fragments were amplified using the polymerase chain reaction from two samples of artisan fresh cheese and a sample of irrigation water from the vegetable garden. A control case study was carried out, and the variable cow's artisan fresh cheese consumption was statistically significant (p = .01). Conclusions: The results showed that cheese analysed and/or irrigated water of vegetable represented an important route of transmission for the disease. This is the first reported outbreak possibly caused by cow's artisan fresh cheese. It is difficult to prove that these routes were the cause of the outbreak; however, the findings allow us to infer that the individuals involved in the outbreak were in contact with these risk factors.
Emerging Infectious Diseases, 2019
Revista Brasileira de Parasitologia Veterinária, 2019
The objective of this study was to report an outbreak of human toxoplasmosis at a research instit... more The objective of this study was to report an outbreak of human toxoplasmosis at a research institution in Londrina, Paraná, from December 2015 to February 2016. Blood samples from 26 symptomatic individuals were collected and the microparticle chemiluminescence immunoassay was performed to detect IgM, IgG and specific IgG avidity test in the official laboratory. A total of 20 people with symptoms and serology compatible with acute toxoplasmosis (IgM positive and IgG with low avidity) were selected as cases, while 45 asymptomatic employees working in the same teams and during the same shifts were selected as controls. All the participants of the investigation answered an epidemiological questionnaire. Three samples of water and one sludge from the institution's supply cisterns, 10 soil samples, 11 plant samples, three cat fecal samples and one domestic feline cadaver were collected for analysis of the polymerase chain reaction (PCR) for T. gondii. After analyzing the epidemiologi...
Biotemas, 2019
O estudo foi realizado entre julho de 2014 e maio de 2016, em 21 propriedades hortícolas do estad... more O estudo foi realizado entre julho de 2014 e maio de 2016, em 21 propriedades hortícolas do estado do Paraná, Brasil. Foram coletadas duas amostras de vegetais folhosos e uma amostra de água de irrigação por propriedade. As amostras de água foram analisadas pela técnica do substrato cromogênico para avaliar a contaminação por coliformes totais e Escherichia coli, e os vegetais foram avaliados pelas técnicas de Willis (1921), Hoffman et al. (1934), Faust et al. (1939) e quanto à contaminação por parasitas. Observou-se presença de E. coli em 80,95% (17/21) das amostras de água; com relação aos vegetais, 19 (45,23%) continham pelo menos uma espécie de parasita, tais como: ancilostomatídeos, Chilomatix spp., Dipillidium spp., Entamoeba spp., Strongyloides spp., Trichuris spp., larva de vida livre, larva de nematódeo, oocisto não-esporulado. Houve associação estatística entre o destino do esgoto (fossa seca) e a positividade aos parasitas. Os dados mostram contaminação fecal em número si...
Revista Brasileira de Parasitologia Veterinária, 2019
This study evaluated the seroprevalence of Toxoplasma gondii, Neospora caninum and Leptospira spp... more This study evaluated the seroprevalence of Toxoplasma gondii, Neospora caninum and Leptospira spp. in dogs from Foz do Iguaçu, Paraná, Brazil. Indirect immunofluorescent antibody test was used to detect antibodies anti-T.gondii and anti-N. caninum. Immunoenzymatic assay and microscopic serum agglutination were used for screening antibodies anti-T.gondii and anti-Leptospira spp., respectively. The results were: 67.02% of the samples reactive for T.gondii and 1.38% for N. caninum, both without statistically significant variables. For Leptospira spp. the results indicated seroprevalence of 23.11%. The analysis of the variables without distinction of serovar showed association for intrinsic characteristics as breed, age, nutritional status and dog category. The extrinsic variables as city region and access to the street presented association (p<0.05). The most prevalent serovars were: Canicola 59.47%; Bratislava 13.07% and Butembo 15.68%. Variables that make up the adjusted multiple ...
PLOS ONE, 2019
Human toxoplasmosis, a protozoonosis caused by Toxoplasma gondii, has been described as a worldwi... more Human toxoplasmosis, a protozoonosis caused by Toxoplasma gondii, has been described as a worldwide foodborne disease with important public health impact. Despite infection has reportedly varied due to differences in alimentary, cultural and hygienic habits and geographic region, social vulnerability influence on toxoplasmosis distribution remains to be fully established. Accordingly, the present study has aimed to assess T. gondii seroprevalence and factors associated to social vulnerability for infection in households of Ivaiporã, southern Brazil, with 33.6% population making half minimum wage or less, ranked 1,055 th in population (31,816 habitants), 1,406 th in per capita income (U$ 211.80 per month) and 1,021 st in HDI (0.764) out of 5,570 Brazilian cities. Serum samples and epidemiological questionnaires were obtained from citizen volunteers with official City Secretary of Health assistance in 2015 and 2016. In overall, serosurvey has revealed 526/715 (73.57%) positive samples for anti-T. gondii antibodies by Indirect Fluorescent Antibody Test. Logistic regression has shown a significant increase associated to adults (p = 0.021) and elderly (p = 0.014) people, illiterates (p = 0.025), unemployment (p <0.001) and lack of household water tank (p = 0.039). On the other hand, sex (male or female), living area (urban or rural), yard hygiene, meat ingestion, sand or land contact, owning pets (dog, cat or both) were not significant variables of positivity for anti-T. gondii antibodies in the surveyed population. Although no significant spatial cluster was found, high intensity areas of seropositive individuals were located in the Kernel map where the suburban neighborhoods are located. In conclusion, socioeconomic vulnerability determinants may be associated to Toxoplasma gondii exposure. The increased risk due to illiteracy, adult or elderly age, unemployment and lack of household water tank were confirmed by multivariate analysis and the influence of low family income for seropositivity by the spatial analysis.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, Jan 30, 2018
The objective of this study was to determine factors associated with vegetable contamination with... more The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive sa...
to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and conge... more to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and congenital toxoplasmosis in a Sentinel Unit (SU) and describe reported cases. this was a descriptive study of the implantation of a notification protocol using a specific notification form for suspected cases of toxoplasmosis in pregnant women and congenital toxoplasmosis in a reference center in Paraná State, Brazil, from August 2013 to August 2014. all 64 suspected cases were notified and case investigation was completed by the SU Epidemiology Sector; 63 received prenatal care and 51 received treatment during pregnancy; 7 of the children being clinically monitored had confirmed diagnosis of congenital toxoplasmosis. implanting toxoplasmosis notification afforded epidemiological, clinical and diagnostic data on the disease that contributed to the assessment of the clinical evolution of children exposed to Toxoplasma gondii.
Brazilian Journal of Infectious Diseases, Sep 1, 2014
Malaria is endemic in the North of Brazil. However, Hyperreactive Malarious Splenomegaly (HMS) ha... more Malaria is endemic in the North of Brazil. However, Hyperreactive Malarious Splenomegaly (HMS) has been rarely described. Splenomegaly in HIV/Aids infection has a large differential diagnosis, but malaria is a cause of gross splenomegaly, regardless of the HIV status. In this paper, we report the case of a 50-year-old man, HIV positive, with massive splenomegaly and multiple malaria infections in the past. He fulfilled the criteria for HMS, received a short course of anti-malarial treatment and weekly quimioprofilatic Chloroquine. In 9 months, he had great clinical and laboratorial improvement confirming the HMS, a rare diagnosis in Brazil.
Revista de Ciência Veterinária e Saúde Pública, Oct 6, 2016
International journal of epidemiologic research, 2017
Background and aims: We investigated the children of Parana State, Brazil the prevalence of intes... more Background and aims: We investigated the children of Parana State, Brazil the prevalence of intestinal parasitosis and the associated factors involved in the transmission of intestinal parasites, and we genotyped the Giardia duodenalis isolates obtained. Methods: Fecal samples were analyzed by established microscopic methods. G. duodenalis positive samples were subjected to genotypic characterization by PCR amplification of sequences of the glutamate dehydrogenase gene (gdh) and by enzymatic digestion with the restriction enzyme NlaIV for classification of genotypes. Results: Of the 877 samples tested, 41% were positive for some intestinal parasitosis, the most common being the presence of protozoa (87.8%). Lack of basic sanitation and poor health education were associated for the intestinal parasite cases found, and the only associated factor for giardiasis was low family income. The G. duodenalis assemblages of gdh amplified samples were 68.6% B and 31.4% AII. Conclusion: These da...
Semina: Ciências Agrárias, 2017
The objective of this study was to investigate the presence of anti-Leishmania spp. antibodies us... more The objective of this study was to investigate the presence of anti-Leishmania spp. antibodies using an indirect immunofluorescence technique in the serum of equidae slaughtered in two slaughterhouses registered with the Federal Inspection Service. Titers greater than 40 were considered reagents. Blood samples were collected from 398 animals of both sexes, with different ages, and coming from six Brazilian states, of which 46.0% (183/398) were reactive, indicating that these animals were exposed to the leishmaniasis agent that is widely distributed in Brazil.
Sao Paulo Medical Journal, 2020
The transmission routes for toxoplasmosis include consumption of raw foods, undercooked meats and... more The transmission routes for toxoplasmosis include consumption of raw foods, undercooked meats and untreated water; contact with soil contaminated with the evolutionary infective forms of the protozoon Toxoplasma gondii (T. gondii); and transplacental infection. 1 Differences in pregnant women's food intake and cultural habits around the world are responsible for variations in the prevalence of congenital toxoplasmosis among countries, 2 and between communities within the same nation. 3 Preventive measures are common to all populations, since they concentrate on health education and early detection of infection during pregnancy. 4 However, the public health strategies for prevention of this parasitosis need to be appropriate for each situation. T. gondii infection is a major problem within gestational health because of the possibility of irreversibly damaging the fetus. 1 The main types of damage reported have included chorioretinitis, deafness, intracranial calcification, microcephaly, hydrocephalus, seizures and intellectual disability. In addition, occurrences of spontaneous abortions, neonatal death and neurodevelopmental disorders have been recorded. 5 Researchers from the World Health Organization (WHO) reported that in 2010, despite neglect due to little attention given to the prevalence, prevention and treatment of toxoplasmosis, 10.3 million cases of this disease were recorded worldwide, with 825,000 Disability-Adjusted Life Years (DALYs), one of the highest among foodborne parasitic diseases. 2 The Brazilian strains of T. gondii are highly damaging to the eye tissue, in comparison with strains in other countries. 6 However, reduction of the incidence of congenital infection, through early identification of maternal infection and adoption of strategic therapy for pregnant women and
Preventive Veterinary Medicine, 2020
Transboundary and Emerging Diseases, 2020
Toxoplasmosis and neosporosis are diseases caused by the protozoa Toxoplasma gondii and Neospora ... more Toxoplasmosis and neosporosis are diseases caused by the protozoa Toxoplasma gondii and Neospora caninum, respectively. They may be associated with reproductive and economic losses due to infection, resulting from inadequate management practices in ovine herds. Toxoplasma gondii is one of the major parasitic aetiological agents involved in abortion of sheep and goats in the USA and Scotland (Dubey & Kirkbride 1984). Additionally, Larsson, Jamra, Guimarães, Pattoli, and Silva (1980) have verified the presence of antibodies against T. gondii in sheep herds from the city of Uruguaiana, Rio Grande do Sul (RS), Brazil. Moreover, we have previously (Romanelli et al., 2007) investigated the occurrence of N. caninum in sheep from the state of Paraná, where a prevalence of 9.5% (29/305) was reported, but risk factors associated with seropositivity were not identified. Pinto et al., (2012) demonstrated N. caninum as the primary
Medicina Veterinária (UFRPE), 2017
Toxoplasmosis is a zoonosis caused by the Toxoplasma gondii protozoa. The congenital form happens... more Toxoplasmosis is a zoonosis caused by the Toxoplasma gondii protozoa. The congenital form happens when the parasite reaches the fetus through the placenta and causes damages of different intensities, which can be consequence of the strain virulence, of the immune response capability of the pregnant woman or even of the pregnancy stage, and can result in fetal death or severe clinical symptomatology. The aim of this paper was to determine the prevalence of antibodies to Toxoplasma gondii antibodies in pregnant woman seen at the Basic Health Units (BHU) in the city of Umuarama (PR) and check for possible toxoplasmosis associations with social-demographic characteristics, behavior and environmental variables. In this study, a total of 813 pregnant women who had their prenatal exams at the BHU in the city of Umuarama (PR) were included. Blood samples collected from these subjects were submitted to IgG and IgM anti-T. gondii antibody survey. Each pregnant woman answered an epidemiologica...
Zoonoses and Public Health, 2019
Background: The objective of this study was to report an outbreak of human toxoplasmosis occurrin... more Background: The objective of this study was to report an outbreak of human toxoplasmosis occurring in the municipality of Montes Claros de Goiás, Goiás, Brazil, from December 2015 to August 2016. Seven acute cases in June 2016 triggered the subsequent search. Methods: A total of 251 individuals were selected through an active search, of which 114 (45.4%) agreed to participate in the research and blood collection. For serological diagnosis were used enzyme-linked immunosorbent assay (ELISA) for IgG and IgM and avidity tests. Results: Of the 114 serum samples evaluated, 12.3% (14/114) showed antibodies against Toxoplasma gondii, with a profile indicative of acute infection. Samples of artisan fresh cheese, public water, vegetables and irrigation water were collected. Toxoplasma gondii DNA fragments were amplified using the polymerase chain reaction from two samples of artisan fresh cheese and a sample of irrigation water from the vegetable garden. A control case study was carried out, and the variable cow's artisan fresh cheese consumption was statistically significant (p = .01). Conclusions: The results showed that cheese analysed and/or irrigated water of vegetable represented an important route of transmission for the disease. This is the first reported outbreak possibly caused by cow's artisan fresh cheese. It is difficult to prove that these routes were the cause of the outbreak; however, the findings allow us to infer that the individuals involved in the outbreak were in contact with these risk factors.
Emerging Infectious Diseases, 2019
Revista Brasileira de Parasitologia Veterinária, 2019
The objective of this study was to report an outbreak of human toxoplasmosis at a research instit... more The objective of this study was to report an outbreak of human toxoplasmosis at a research institution in Londrina, Paraná, from December 2015 to February 2016. Blood samples from 26 symptomatic individuals were collected and the microparticle chemiluminescence immunoassay was performed to detect IgM, IgG and specific IgG avidity test in the official laboratory. A total of 20 people with symptoms and serology compatible with acute toxoplasmosis (IgM positive and IgG with low avidity) were selected as cases, while 45 asymptomatic employees working in the same teams and during the same shifts were selected as controls. All the participants of the investigation answered an epidemiological questionnaire. Three samples of water and one sludge from the institution's supply cisterns, 10 soil samples, 11 plant samples, three cat fecal samples and one domestic feline cadaver were collected for analysis of the polymerase chain reaction (PCR) for T. gondii. After analyzing the epidemiologi...
Biotemas, 2019
O estudo foi realizado entre julho de 2014 e maio de 2016, em 21 propriedades hortícolas do estad... more O estudo foi realizado entre julho de 2014 e maio de 2016, em 21 propriedades hortícolas do estado do Paraná, Brasil. Foram coletadas duas amostras de vegetais folhosos e uma amostra de água de irrigação por propriedade. As amostras de água foram analisadas pela técnica do substrato cromogênico para avaliar a contaminação por coliformes totais e Escherichia coli, e os vegetais foram avaliados pelas técnicas de Willis (1921), Hoffman et al. (1934), Faust et al. (1939) e quanto à contaminação por parasitas. Observou-se presença de E. coli em 80,95% (17/21) das amostras de água; com relação aos vegetais, 19 (45,23%) continham pelo menos uma espécie de parasita, tais como: ancilostomatídeos, Chilomatix spp., Dipillidium spp., Entamoeba spp., Strongyloides spp., Trichuris spp., larva de vida livre, larva de nematódeo, oocisto não-esporulado. Houve associação estatística entre o destino do esgoto (fossa seca) e a positividade aos parasitas. Os dados mostram contaminação fecal em número si...
Revista Brasileira de Parasitologia Veterinária, 2019
This study evaluated the seroprevalence of Toxoplasma gondii, Neospora caninum and Leptospira spp... more This study evaluated the seroprevalence of Toxoplasma gondii, Neospora caninum and Leptospira spp. in dogs from Foz do Iguaçu, Paraná, Brazil. Indirect immunofluorescent antibody test was used to detect antibodies anti-T.gondii and anti-N. caninum. Immunoenzymatic assay and microscopic serum agglutination were used for screening antibodies anti-T.gondii and anti-Leptospira spp., respectively. The results were: 67.02% of the samples reactive for T.gondii and 1.38% for N. caninum, both without statistically significant variables. For Leptospira spp. the results indicated seroprevalence of 23.11%. The analysis of the variables without distinction of serovar showed association for intrinsic characteristics as breed, age, nutritional status and dog category. The extrinsic variables as city region and access to the street presented association (p<0.05). The most prevalent serovars were: Canicola 59.47%; Bratislava 13.07% and Butembo 15.68%. Variables that make up the adjusted multiple ...
PLOS ONE, 2019
Human toxoplasmosis, a protozoonosis caused by Toxoplasma gondii, has been described as a worldwi... more Human toxoplasmosis, a protozoonosis caused by Toxoplasma gondii, has been described as a worldwide foodborne disease with important public health impact. Despite infection has reportedly varied due to differences in alimentary, cultural and hygienic habits and geographic region, social vulnerability influence on toxoplasmosis distribution remains to be fully established. Accordingly, the present study has aimed to assess T. gondii seroprevalence and factors associated to social vulnerability for infection in households of Ivaiporã, southern Brazil, with 33.6% population making half minimum wage or less, ranked 1,055 th in population (31,816 habitants), 1,406 th in per capita income (U$ 211.80 per month) and 1,021 st in HDI (0.764) out of 5,570 Brazilian cities. Serum samples and epidemiological questionnaires were obtained from citizen volunteers with official City Secretary of Health assistance in 2015 and 2016. In overall, serosurvey has revealed 526/715 (73.57%) positive samples for anti-T. gondii antibodies by Indirect Fluorescent Antibody Test. Logistic regression has shown a significant increase associated to adults (p = 0.021) and elderly (p = 0.014) people, illiterates (p = 0.025), unemployment (p <0.001) and lack of household water tank (p = 0.039). On the other hand, sex (male or female), living area (urban or rural), yard hygiene, meat ingestion, sand or land contact, owning pets (dog, cat or both) were not significant variables of positivity for anti-T. gondii antibodies in the surveyed population. Although no significant spatial cluster was found, high intensity areas of seropositive individuals were located in the Kernel map where the suburban neighborhoods are located. In conclusion, socioeconomic vulnerability determinants may be associated to Toxoplasma gondii exposure. The increased risk due to illiteracy, adult or elderly age, unemployment and lack of household water tank were confirmed by multivariate analysis and the influence of low family income for seropositivity by the spatial analysis.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, Jan 30, 2018
The objective of this study was to determine factors associated with vegetable contamination with... more The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive sa...