Fidele Assogba | Université d'abomey-Calavi (original) (raw)
Papers by Fidele Assogba
Pharmaceutical Biology, 2001
... Biosciences Res Commun 4: 113117. Olubadewo IO, Ochillo RF, Crockett CO, Guede-Guina F, Pugh... more ... Biosciences Res Commun 4: 113117. Olubadewo IO, Ochillo RF, Crockett CO, Guede-Guina F, Pugh D, Vangah-Manda M, Robinson TI (1992): Cassia alata and the preclinical search for therapeutic agents for the treatment of opportunistic infections in AIDS patients. ...
Journal of Applied Biosciences, Apr 19, 2017
Objectives: Sorghum bicolor is a major grain crop for human and animal nutrition in Africa and In... more Objectives: Sorghum bicolor is a major grain crop for human and animal nutrition in Africa and India. Its leaves are used to treat anaemia. This work aimed to study it biological tolerance. Methodology and Results: In the aqueous extract of the leaves of the plant were conducted larval toxicity test and the acute oral toxicity according to guideline 423 of the OECD. Wistar rats were injected with 2000 mg of extract / kg body weight was followed for 14 days. The D0 and D14, blood tests were performed as well as liver histology, kidney and spleen. In vitro, the LC50 was 7.9 mg / ml. The weight of the rats, blood urea, creatinine, transaminases and the leukocyte count did not change significantly on day 14, suggesting the absence of renal, hepatic and immunological toxicity, confirmed by Conclusion and application of results: Sorghum bicolor aqueous extract showed no toxicity in vitro to the larvae. In vivo, it did not induce hepatic cytolysis, liver enzyme AST and ALT slightly decreased. It did not alter renal function, blood urea and creatinine levels were normal. The structure of these two organs seems to be preserved at histological observation. In addition, the extract did not appear to affect immune function since the count of white blood cells was normal. The parenchyma of the spleen, lymphoid organ kept its typical architecture. The study of biological tolerance should be continued by chronic toxicity tests and appropriate clinical trials for a transformation into Improved Traditional Medicine (ITM).
American Journal of Applied Chemistry, 2020
Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in lit... more Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in literature for its biological properties. Aims were to study its phytochemical composition and some biological properties and influence of acid hydrolysis on biological activities and chemical composition of Croton lobatus L. extracts. Successive extractions with solvents of increasing polarity (cyclohexane, ethyl acetate, and methanol) were carried out on powder of Croton lobatus L. leaves. Evaluation of xanthine oxidase, acetylcholinesterase, 5-lipoxygenase and α-amylase inhibitions, antioxidant activity was performed. Acid hydrolysis effect was evaluated by comparison of chemical composition and xanthine oxidase, α-amylase inhibition activities of hydrolyzed and unhydrolyzed extracts. Results showed that at 50 mg/L Croton lobatus L. has very small antioxidant activity (3.4-4.9%), small inhibitory activity of 5-lipoxygenase (6.3-6.9%), inhibitory activity of acetylcholinesterase between (2.7-31.3%), moderate inhibitory activity of α-amylase (52.8-64.3%), moderate inhibitory activity of xanthine oxidase (22.2-62.6%). Croton lobatus L. could be used in diabetes and gout treatments, given results of α-amylase inhibition and xanthine oxidase. Acid hydrolysis has negative effect on process of xanthine oxidase inhibition and on chemical composition.
Journal of Applied Biosciences, Mar 30, 2017
Ethnobotanical and phytochemical study of Momordica charantia Linn (Cucurbitaceae) in Cotonou Ben... more Ethnobotanical and phytochemical study of Momordica charantia Linn (Cucurbitaceae) in Cotonou Benin ABSTRACT Objective: This study, on Momordica charantia Linn (Cucurbitaceae), commonly known as margose a plant widely used for the treatment of many diseases, is conducted in. Cotonou the capital city of Benin. It aims to verify the scientific scope of the empirical uses of this plant.and to promote its safe use Methodology and Results: An ethnobotanical study was conducted among 32 vendors in the three main markets of medicinal plants in Cotonou. In addition, a phytochemical exploration, associated with the determination of heavy metals and some nutrients were also been conducted on this plant. During the ethnobotanical study the known virtues of the plant , are correlated with the active fractions revealed by the phytochemical screening (tannins, flavonoids, anthracene derivatives, alkaloids, steroids, and mucilage). Lead and Cadmium were also detected with concentrations of about nine and six times, respectively, higher than the World Health Organization standards for human nutrition. Conclusion and application of results: This study revealed the correlation between the empirical uses of Mormodica Charantia and the phytochemical study. This study results highlight the importance to establish a database for validation of traditional knowledge of population related to the plants uses However, this research shows the risk of contamination with lead and cadmium which the population is subjected in the use of. Momordica charantia.
Journal of Applied Biosciences, Apr 19, 2017
Journal of Pharmacognosy and Phytochemistry, 2018
Diarrhoeic diseases caused several million deaths in the world annually, especially in Africa. 52... more Diarrhoeic diseases caused several million deaths in the world annually, especially in Africa. 52 billion FCFA is annual attributed to this disease in Benin. They are very little scientific workonplants which can curediarrhoeic diseases in Benin. The aim of this study is to perform phytochemical screening, toxicological study of aqueous and ethanolic extracts of Mangifera Indica, Cajanus cajan and Piliostigma thonningii leaves and the relationship between the phytochemical compositions. The leaves of this plants contained notably tannins and flavonoid which show good relationship. Aqueous and ethanolic extracts have LD50 ≥ 5000mg / kg w.b and are not toxic on shrimp larvae. Practically no significant effect was observed on rats (hematological and biochemical parameters: P>0.05). This study demonstrated relationship between the phytochemical compositions of the three plants with no toxic effect. Further antidiarrheal studies of these extracts will provide better insights on this plants and endogenous practice.
Journal of Drug Delivery and Therapeutics, Apr 15, 2022
_________________________________________________________________________________________________... more ___________________________________________________________________________________________________________ In the Republic of Benin, several plants, including Zea mays Linn (Z. mays) are used for the treatment of diabetes without any scientific studies showing their effectiveness. The objective of this study is to investigate the effects of Z. mays leaves' extracts on hyperglycaemic rabbits using the Oral Glucose Tolerance Test (OGTT), 2 g/kg of (D) + glucose and on hepatic glucose liberation. Phytochemical screening revealed that the plant leaves contain alkaloids, tannins, mucilage flavonoids, anthocyanin, leuco-anthocyanin, coumarins, heteroside, flavonoid, triterpenoids, steroids, reducing compounds, saponins, oses and holosides. Cytotoxity tests showed that the aqueous and ethanolic extracts were free of toxicity. The extracts have shown anti-hyperglycaemic activities dependent on specific dosage and timing. The effective dose is 500 mg/kg for the aqueous extract and for the ethanolic extract. The extracts are effective as compared with glibenclamide (reference product). Moreover, the ex vivo test conducted on the liver revealed that Z. mays aqueous extract inhibits the hepatic glucose liberation and 500 mg/kg is the most effective dose. The results of this study justify the traditional usage of the plant leaves under consideration in the treatment of diabetes.
Journal of Pharmacognosy and Phytochemistry, 2020
As part of the development of traditional medicine rich in Africa and particularly in Benin, seve... more As part of the development of traditional medicine rich in Africa and particularly in Benin, several studies have been conducted for years to evaluate the ethnopharmacological properties of medicinal plants. It is in this context that Mitragyna inermis is known for many of these properties mentioned in the traditional pharmacopoeia and whose biological analyses have confirmed some of them. The purpose of this work is to summarise previous work; biological as chemical on this plant. The extracts showed that it has antibacterial, antiviral, antiparasitic properties. The isolated compounds are certainly responsible for these known biological activities. The synergistic action of the compounds present in the extracts can justify also its use in the treatment of several pathologies. Finally, this review of literature carried out on this plant, is a contribution to the synthesis of the previous works carried out in order to deepen its valuation.
European Scientific Journal, ESJ, 2018
The most cited plant drugs during an ethnobotanical survey conducted for traditional healers and ... more The most cited plant drugs during an ethnobotanical survey conducted for traditional healers and resource persons in southern Benin, used in the treatment of female infertility, were the subject of a phytochemical screening. It aims to determine the chemical substances that are conferred on the plants. These chemical substances are the pharmacological properties that are used for the treat of ailment recognized as being the origin of this disease. As a result, we studied the cytotoxicity of each herbal drug. In total, the organs of 17 plant species involved in this study are Aframomum melegueta, Allium cepa, Anchomanes deformants, Baphia nitida, Carissa spinarum, Elaeis guineensis, Garcinia cola, Kigelia africana, Monodora myristica, Morinda lucida, Musa sapientum, Olax subscorpioidea, Piper guineense, Pterocarpus erinaceus, Pupalia lappacea, Tetrapleura tetraptera, Xylopia aethiopica. The results obtained indicate that the richness in phytochemical element is a function of the spec...
International Journal of Biology, 2016
Anaemia is a serious public health problem especially in developing countries as Benin. Cocos nuc... more Anaemia is a serious public health problem especially in developing countries as Benin. Cocos nucifera is one of medicinal plants used in Benin to treat anemia. This study aimed to test its therapeutic efficacy in anemia treatment. Method: Five groups of five Wistar rats each were formed. The rats of four groups were rendered anemic by injection of phenylhydrazine (hemolysis) in the first two days D0 and D1. From the second to the fifteenth day (D2 to D15), anemic groups were gavaged either by the aqueous extract of Cocos nucifera at 200 or 300 mg / kg body weight/day, or by vitafer, a reference drug against anemia. The last anemic group was not treated. The group of non-anemic rats served as a control. Blood samples were collected for all rats on days D0, D2, D7, D10 and D15 to assess blood count and osmotic resistance of red blood cells. Results: The phytochemical analysis revealed the presence of tannins, flavonoids, leucoanthocyanes, steroids, quinone derivatives, reducing compo...
Food and Nutrition Sciences, 2012
Vegetables are very important sources of protein and minerals. Some of them even have medicinal p... more Vegetables are very important sources of protein and minerals. Some of them even have medicinal properties recognized traditionally. Despite the large number of studies carried out on various vegetables and vegetable crops, very few have scientifically explored the usefulness of S. macrocarpon. This study identified the main groups of chemicals and mineral elements to explain any medicinal or nutritional value. It has also identified some toxic elements contained in this vegetable. Phytochemical screening was carried out on the leaves and fruits of S. macrocarpon. Some mineral elements were determined by Atomic Absorption Spectrophotometry (sodium, potassium, calcium, magnesium) while protein, phosphorous, iron, copper, zinc and toxic metals (lead, cadmium) were determined by Molecular Absorption Spectrophotometry. Fat, ash, moisture and vitamins were sought. The study showed that the leaves of S. macrocarpon were more nutritious than fruits (P < 0.05). The high protein content of the leaves and fruit suggests an interesting nutritive property. The presence of chemical groups and toxic elements (lead, cadmium) in S. macrocarpon require that the consumption of vegetables should be as varied as possible and that the fruit may be consumed with caution. In addition, constraints about vegetable cultivation in Cotonou could lead to research findings that could help provide techniques for producing healthy vegetables.Vitamins A and K1 were found in both parts of this vegetable very rich in water while vitamin E has not been detected. S. macrocarpon also contains lipids at various levels.
Ocimum gratissimum has been used in traditional medicine for curing various ailments in tropical ... more Ocimum gratissimum has been used in traditional medicine for curing various ailments in tropical countries. The plant with unique bioactive compounds possesses a significant medicinal value. Among numerous curative properties the plant shows strong inhibition against human viruses and parasitic protozoans. The presence of eugenol makes it a safe mosquito repellent and anti-malarial agent. Moreover, the plant causes mortality of agricultural insects in both store seed and field crops. The researches have also proved the inhibitory action of plants against various plants and human nematodes harboring alimentary canal. Thus, there is a need to explore the potentiality of this plant to discover biological drug formulation with relevant action against plant and animal microbes, malaria parasites and nematodes. This systematic review presents the antiviral, antiprotozoal, antimalarial, insecticides and nematicidal activities of the plant for developing a standard therapeutic system.
Melaleuca quinquenervia is a plant well-known for its medicinal properties. Its leaves are mainly... more Melaleuca quinquenervia is a plant well-known for its medicinal properties. Its leaves are mainly used for the treatment of respiratory diseases. The present study aims was to appreciate the anti-inflammatory properties of the essential oil from the leaves of Melaleuca quinquenervia (Cav.) ST Blake Myrtaceae (Niaouli). Phytochemical analysis of the essential oil, by CG/MS, revealed the major constituents such as: 1,8-cineole (30.14%), viridiflorol (29.92%), terpinene-4-ol (10.12%) α-terpineol (6.89%), limonene (4.36%). The study of the anti-inflammatory activity of this essential oil on wistar rats showed a decrease in the rate of the oedema evolution and displayed significant dose and time dependent of this oedema inhibition. The essential oil showed also strong analgesic and antipyretic properties. The dose of 1800 mg/kg showed a better activity than that induced by 50 mg/kg of acetylsalicylate of lysine. Altogether, this work demonstrates the anti-inflammatory property of Melaleu...
Journal of chemical and pharmaceutical research, 2015
The main aim of the present investigation was to ap preciate the diuretic and natridiureticactivi... more The main aim of the present investigation was to ap preciate the diuretic and natridiureticactivities a nd the toxicity from Elaeis guineensis leaves aqueous extract (EGLA E). fter orally administration of the aqueous extr act at doses of 67.5 mg/kg, 135 mg/kg and 202.50 mg/kg (body wei ght) to Wistar rat, the volume of urine excreted an d the quantity of ions (Na , K) are respectively determined in stepped up test-tu be and by ionic spectrophotometrically measuring. The results show that the urine of Wista r rathas a pH between 6.1 and 8.6 with an average o f 7.35 ± 1.25. Diuresis basic after six hours is 7.8 mL. A s ignificant diuretic activities (157.16%, 169.25%) a reobserved in Wistar rattreated respectively with 135 mg/kg of EG LAE and furosemide (as reference drug, 20 mg/kg) an d modest diuretic activity (146.32%) with a dose of 2 02.50 mg/kg of EGLAE. Itnatridiuretic activity was 2.34 at same dose of 135 mg/kg against 2.52 for furosemide (20 m g/kg) and 0.75 for distilled wa...
International Journal of Innovation and Applied Studies, 2016
The use of biodegradable food packaging plants could provide an alternative to plastic packaging,... more The use of biodegradable food packaging plants could provide an alternative to plastic packaging, particularly for some traditional foods. This work aims to investigate the species of plant leaves used as food packaging in the food crafts in West Africa through their domestication. Data on different species of plant leaves have been collected through a survey among 8 municipalities in Benin using tools and methods based on focus groups. The criteria to select priority species for domestication have been identified from the pair wise comparison of plant species identified in each locality, using standard method. A larval toxicity test was performed on the main species to ensure their safety for domestication. A total of 43 plant species belonging to 29 families have been identified. From them, about 20% was domesticated and 80% were grown naturally. A total of 21.5% of natural species are in the way to be domesticated from which ten (10) have been identified as priority, non-toxic wi...
European Journal of Medicinal Plants, 2021
Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat... more Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract whil...
British Biotechnology Journal, 2015
The use of plants in traditional medicine become very common nowadays throughout the world and in... more The use of plants in traditional medicine become very common nowadays throughout the world and in developing countries in particular. The current study was carried out aiming to compare the chemical features of three vegetables (Vernonia amygdalina, Crateva adansonii and Sesamum radiatum) mostly used for human consumption and traditional medicine to treat bacterial diarrhoea in Benin. These vegetables were selected among the 27 species obtained after ethno-botanical investigations in southern Benin. Therefore, the major chemical groups contained in these vegetables were detected by solubility assays together with coloration and precipitation reactions. Furthermore, cytotoxicity of the plant extracts was assessed on shrimp larvae (Artemia salina). The results showed that gallic tannins, flavonoids, alkaloids, mucilages, coumarins and reducing compounds were detected in all samples (100%) followed by cathechic tannins (66.66%) and saponins (33.33%). The study also revealed that none of the studied vegetables is cytotoxic (LC 50 > 0.01 mg/ml). This study was conducted in the perspective of establishing innovations capable of assisting to; manufacture Improved Traditional Medicines for the treatment of bacterial diarrhoea in the future. Besides, it pointed out the presence of secondary metabolites and the cytotoxicity of these vegetables interesting for further antibacterial, antidiarrheal and pharmacological studies.
The comparative study of physico-chemical characteristics of Terminalia catappa L. kernel oils ex... more The comparative study of physico-chemical characteristics of Terminalia catappa L. kernel oils extracted by two methods has been done. The oil yields were 28.13 % and 61.78 % respectively for traditional and hexane methods and the fatty acid profiles showed palmitic acid (40.79 % and 40.03 % respectively) oleic acid (25.55 % and 26.09 % respectively), linoleic acid (26.72 % and 26.64 % respectively) and stearic acid (4.35 % and 4.49 % respectively) as major components. The oils extracted by the two ways showed similar physico-chemical properties, good calorific values and non-toxicity against Artemia salina L. Oil obtained by traditional method exhibited more antioxidant capacity (1.40) than the hexane one (0.15). This traditional method helps to extract 45 % of the total oil. It gives oil free of organic solvent, with good physico-chemical properties that could be useful as edible oil and for industrial applications.
Vigna comosa Baker (V. comosa) is a plant used by Beninese Oueme department's population for the ... more Vigna comosa Baker (V. comosa) is a plant used by Beninese Oueme department's population for the treatment of diabetes without any scientific study showing its effectiveness. The objective of this study is to investigate the effects of the extracts of V. comosaon hyperglycemic rabbits by Oral Glucose Tolerance Test (OGTT) and on hepatic glucose liberation. Phytochemical screening revealed that the plant contains alkaloids, tannins, flavonoids, mucilage, triterpenoids, steroids, reducing compounds, saponins, oses and holosides. Cytotoxity test showed that those extracts were free of toxicity. The extracts showed anti-hyperglycemic activities doses and time depending. The effective doses are 500 mg/kg for the aqueous extract and 1000 mg/kg for the ethanolic extract. The extracts are effective as compared with glibenclamide (reference product). Moreover, the ex vivo test conducted on the liver revealed that V. comosa aqueous extract inhibits the hepatic glucose liberation and 500 mg/mL is the most effective dose. The results of this study account for the plant under consideration of its traditional usage in the treatment of diabetes.
Pharmaceutical Biology, 2001
... Biosciences Res Commun 4: 113117. Olubadewo IO, Ochillo RF, Crockett CO, Guede-Guina F, Pugh... more ... Biosciences Res Commun 4: 113117. Olubadewo IO, Ochillo RF, Crockett CO, Guede-Guina F, Pugh D, Vangah-Manda M, Robinson TI (1992): Cassia alata and the preclinical search for therapeutic agents for the treatment of opportunistic infections in AIDS patients. ...
Journal of Applied Biosciences, Apr 19, 2017
Objectives: Sorghum bicolor is a major grain crop for human and animal nutrition in Africa and In... more Objectives: Sorghum bicolor is a major grain crop for human and animal nutrition in Africa and India. Its leaves are used to treat anaemia. This work aimed to study it biological tolerance. Methodology and Results: In the aqueous extract of the leaves of the plant were conducted larval toxicity test and the acute oral toxicity according to guideline 423 of the OECD. Wistar rats were injected with 2000 mg of extract / kg body weight was followed for 14 days. The D0 and D14, blood tests were performed as well as liver histology, kidney and spleen. In vitro, the LC50 was 7.9 mg / ml. The weight of the rats, blood urea, creatinine, transaminases and the leukocyte count did not change significantly on day 14, suggesting the absence of renal, hepatic and immunological toxicity, confirmed by Conclusion and application of results: Sorghum bicolor aqueous extract showed no toxicity in vitro to the larvae. In vivo, it did not induce hepatic cytolysis, liver enzyme AST and ALT slightly decreased. It did not alter renal function, blood urea and creatinine levels were normal. The structure of these two organs seems to be preserved at histological observation. In addition, the extract did not appear to affect immune function since the count of white blood cells was normal. The parenchyma of the spleen, lymphoid organ kept its typical architecture. The study of biological tolerance should be continued by chronic toxicity tests and appropriate clinical trials for a transformation into Improved Traditional Medicine (ITM).
American Journal of Applied Chemistry, 2020
Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in lit... more Croton lobatus L. is medicinal plant widely used in traditional medicine, but little known in literature for its biological properties. Aims were to study its phytochemical composition and some biological properties and influence of acid hydrolysis on biological activities and chemical composition of Croton lobatus L. extracts. Successive extractions with solvents of increasing polarity (cyclohexane, ethyl acetate, and methanol) were carried out on powder of Croton lobatus L. leaves. Evaluation of xanthine oxidase, acetylcholinesterase, 5-lipoxygenase and α-amylase inhibitions, antioxidant activity was performed. Acid hydrolysis effect was evaluated by comparison of chemical composition and xanthine oxidase, α-amylase inhibition activities of hydrolyzed and unhydrolyzed extracts. Results showed that at 50 mg/L Croton lobatus L. has very small antioxidant activity (3.4-4.9%), small inhibitory activity of 5-lipoxygenase (6.3-6.9%), inhibitory activity of acetylcholinesterase between (2.7-31.3%), moderate inhibitory activity of α-amylase (52.8-64.3%), moderate inhibitory activity of xanthine oxidase (22.2-62.6%). Croton lobatus L. could be used in diabetes and gout treatments, given results of α-amylase inhibition and xanthine oxidase. Acid hydrolysis has negative effect on process of xanthine oxidase inhibition and on chemical composition.
Journal of Applied Biosciences, Mar 30, 2017
Ethnobotanical and phytochemical study of Momordica charantia Linn (Cucurbitaceae) in Cotonou Ben... more Ethnobotanical and phytochemical study of Momordica charantia Linn (Cucurbitaceae) in Cotonou Benin ABSTRACT Objective: This study, on Momordica charantia Linn (Cucurbitaceae), commonly known as margose a plant widely used for the treatment of many diseases, is conducted in. Cotonou the capital city of Benin. It aims to verify the scientific scope of the empirical uses of this plant.and to promote its safe use Methodology and Results: An ethnobotanical study was conducted among 32 vendors in the three main markets of medicinal plants in Cotonou. In addition, a phytochemical exploration, associated with the determination of heavy metals and some nutrients were also been conducted on this plant. During the ethnobotanical study the known virtues of the plant , are correlated with the active fractions revealed by the phytochemical screening (tannins, flavonoids, anthracene derivatives, alkaloids, steroids, and mucilage). Lead and Cadmium were also detected with concentrations of about nine and six times, respectively, higher than the World Health Organization standards for human nutrition. Conclusion and application of results: This study revealed the correlation between the empirical uses of Mormodica Charantia and the phytochemical study. This study results highlight the importance to establish a database for validation of traditional knowledge of population related to the plants uses However, this research shows the risk of contamination with lead and cadmium which the population is subjected in the use of. Momordica charantia.
Journal of Applied Biosciences, Apr 19, 2017
Journal of Pharmacognosy and Phytochemistry, 2018
Diarrhoeic diseases caused several million deaths in the world annually, especially in Africa. 52... more Diarrhoeic diseases caused several million deaths in the world annually, especially in Africa. 52 billion FCFA is annual attributed to this disease in Benin. They are very little scientific workonplants which can curediarrhoeic diseases in Benin. The aim of this study is to perform phytochemical screening, toxicological study of aqueous and ethanolic extracts of Mangifera Indica, Cajanus cajan and Piliostigma thonningii leaves and the relationship between the phytochemical compositions. The leaves of this plants contained notably tannins and flavonoid which show good relationship. Aqueous and ethanolic extracts have LD50 ≥ 5000mg / kg w.b and are not toxic on shrimp larvae. Practically no significant effect was observed on rats (hematological and biochemical parameters: P>0.05). This study demonstrated relationship between the phytochemical compositions of the three plants with no toxic effect. Further antidiarrheal studies of these extracts will provide better insights on this plants and endogenous practice.
Journal of Drug Delivery and Therapeutics, Apr 15, 2022
_________________________________________________________________________________________________... more ___________________________________________________________________________________________________________ In the Republic of Benin, several plants, including Zea mays Linn (Z. mays) are used for the treatment of diabetes without any scientific studies showing their effectiveness. The objective of this study is to investigate the effects of Z. mays leaves' extracts on hyperglycaemic rabbits using the Oral Glucose Tolerance Test (OGTT), 2 g/kg of (D) + glucose and on hepatic glucose liberation. Phytochemical screening revealed that the plant leaves contain alkaloids, tannins, mucilage flavonoids, anthocyanin, leuco-anthocyanin, coumarins, heteroside, flavonoid, triterpenoids, steroids, reducing compounds, saponins, oses and holosides. Cytotoxity tests showed that the aqueous and ethanolic extracts were free of toxicity. The extracts have shown anti-hyperglycaemic activities dependent on specific dosage and timing. The effective dose is 500 mg/kg for the aqueous extract and for the ethanolic extract. The extracts are effective as compared with glibenclamide (reference product). Moreover, the ex vivo test conducted on the liver revealed that Z. mays aqueous extract inhibits the hepatic glucose liberation and 500 mg/kg is the most effective dose. The results of this study justify the traditional usage of the plant leaves under consideration in the treatment of diabetes.
Journal of Pharmacognosy and Phytochemistry, 2020
As part of the development of traditional medicine rich in Africa and particularly in Benin, seve... more As part of the development of traditional medicine rich in Africa and particularly in Benin, several studies have been conducted for years to evaluate the ethnopharmacological properties of medicinal plants. It is in this context that Mitragyna inermis is known for many of these properties mentioned in the traditional pharmacopoeia and whose biological analyses have confirmed some of them. The purpose of this work is to summarise previous work; biological as chemical on this plant. The extracts showed that it has antibacterial, antiviral, antiparasitic properties. The isolated compounds are certainly responsible for these known biological activities. The synergistic action of the compounds present in the extracts can justify also its use in the treatment of several pathologies. Finally, this review of literature carried out on this plant, is a contribution to the synthesis of the previous works carried out in order to deepen its valuation.
European Scientific Journal, ESJ, 2018
The most cited plant drugs during an ethnobotanical survey conducted for traditional healers and ... more The most cited plant drugs during an ethnobotanical survey conducted for traditional healers and resource persons in southern Benin, used in the treatment of female infertility, were the subject of a phytochemical screening. It aims to determine the chemical substances that are conferred on the plants. These chemical substances are the pharmacological properties that are used for the treat of ailment recognized as being the origin of this disease. As a result, we studied the cytotoxicity of each herbal drug. In total, the organs of 17 plant species involved in this study are Aframomum melegueta, Allium cepa, Anchomanes deformants, Baphia nitida, Carissa spinarum, Elaeis guineensis, Garcinia cola, Kigelia africana, Monodora myristica, Morinda lucida, Musa sapientum, Olax subscorpioidea, Piper guineense, Pterocarpus erinaceus, Pupalia lappacea, Tetrapleura tetraptera, Xylopia aethiopica. The results obtained indicate that the richness in phytochemical element is a function of the spec...
International Journal of Biology, 2016
Anaemia is a serious public health problem especially in developing countries as Benin. Cocos nuc... more Anaemia is a serious public health problem especially in developing countries as Benin. Cocos nucifera is one of medicinal plants used in Benin to treat anemia. This study aimed to test its therapeutic efficacy in anemia treatment. Method: Five groups of five Wistar rats each were formed. The rats of four groups were rendered anemic by injection of phenylhydrazine (hemolysis) in the first two days D0 and D1. From the second to the fifteenth day (D2 to D15), anemic groups were gavaged either by the aqueous extract of Cocos nucifera at 200 or 300 mg / kg body weight/day, or by vitafer, a reference drug against anemia. The last anemic group was not treated. The group of non-anemic rats served as a control. Blood samples were collected for all rats on days D0, D2, D7, D10 and D15 to assess blood count and osmotic resistance of red blood cells. Results: The phytochemical analysis revealed the presence of tannins, flavonoids, leucoanthocyanes, steroids, quinone derivatives, reducing compo...
Food and Nutrition Sciences, 2012
Vegetables are very important sources of protein and minerals. Some of them even have medicinal p... more Vegetables are very important sources of protein and minerals. Some of them even have medicinal properties recognized traditionally. Despite the large number of studies carried out on various vegetables and vegetable crops, very few have scientifically explored the usefulness of S. macrocarpon. This study identified the main groups of chemicals and mineral elements to explain any medicinal or nutritional value. It has also identified some toxic elements contained in this vegetable. Phytochemical screening was carried out on the leaves and fruits of S. macrocarpon. Some mineral elements were determined by Atomic Absorption Spectrophotometry (sodium, potassium, calcium, magnesium) while protein, phosphorous, iron, copper, zinc and toxic metals (lead, cadmium) were determined by Molecular Absorption Spectrophotometry. Fat, ash, moisture and vitamins were sought. The study showed that the leaves of S. macrocarpon were more nutritious than fruits (P < 0.05). The high protein content of the leaves and fruit suggests an interesting nutritive property. The presence of chemical groups and toxic elements (lead, cadmium) in S. macrocarpon require that the consumption of vegetables should be as varied as possible and that the fruit may be consumed with caution. In addition, constraints about vegetable cultivation in Cotonou could lead to research findings that could help provide techniques for producing healthy vegetables.Vitamins A and K1 were found in both parts of this vegetable very rich in water while vitamin E has not been detected. S. macrocarpon also contains lipids at various levels.
Ocimum gratissimum has been used in traditional medicine for curing various ailments in tropical ... more Ocimum gratissimum has been used in traditional medicine for curing various ailments in tropical countries. The plant with unique bioactive compounds possesses a significant medicinal value. Among numerous curative properties the plant shows strong inhibition against human viruses and parasitic protozoans. The presence of eugenol makes it a safe mosquito repellent and anti-malarial agent. Moreover, the plant causes mortality of agricultural insects in both store seed and field crops. The researches have also proved the inhibitory action of plants against various plants and human nematodes harboring alimentary canal. Thus, there is a need to explore the potentiality of this plant to discover biological drug formulation with relevant action against plant and animal microbes, malaria parasites and nematodes. This systematic review presents the antiviral, antiprotozoal, antimalarial, insecticides and nematicidal activities of the plant for developing a standard therapeutic system.
Melaleuca quinquenervia is a plant well-known for its medicinal properties. Its leaves are mainly... more Melaleuca quinquenervia is a plant well-known for its medicinal properties. Its leaves are mainly used for the treatment of respiratory diseases. The present study aims was to appreciate the anti-inflammatory properties of the essential oil from the leaves of Melaleuca quinquenervia (Cav.) ST Blake Myrtaceae (Niaouli). Phytochemical analysis of the essential oil, by CG/MS, revealed the major constituents such as: 1,8-cineole (30.14%), viridiflorol (29.92%), terpinene-4-ol (10.12%) α-terpineol (6.89%), limonene (4.36%). The study of the anti-inflammatory activity of this essential oil on wistar rats showed a decrease in the rate of the oedema evolution and displayed significant dose and time dependent of this oedema inhibition. The essential oil showed also strong analgesic and antipyretic properties. The dose of 1800 mg/kg showed a better activity than that induced by 50 mg/kg of acetylsalicylate of lysine. Altogether, this work demonstrates the anti-inflammatory property of Melaleu...
Journal of chemical and pharmaceutical research, 2015
The main aim of the present investigation was to ap preciate the diuretic and natridiureticactivi... more The main aim of the present investigation was to ap preciate the diuretic and natridiureticactivities a nd the toxicity from Elaeis guineensis leaves aqueous extract (EGLA E). fter orally administration of the aqueous extr act at doses of 67.5 mg/kg, 135 mg/kg and 202.50 mg/kg (body wei ght) to Wistar rat, the volume of urine excreted an d the quantity of ions (Na , K) are respectively determined in stepped up test-tu be and by ionic spectrophotometrically measuring. The results show that the urine of Wista r rathas a pH between 6.1 and 8.6 with an average o f 7.35 ± 1.25. Diuresis basic after six hours is 7.8 mL. A s ignificant diuretic activities (157.16%, 169.25%) a reobserved in Wistar rattreated respectively with 135 mg/kg of EG LAE and furosemide (as reference drug, 20 mg/kg) an d modest diuretic activity (146.32%) with a dose of 2 02.50 mg/kg of EGLAE. Itnatridiuretic activity was 2.34 at same dose of 135 mg/kg against 2.52 for furosemide (20 m g/kg) and 0.75 for distilled wa...
International Journal of Innovation and Applied Studies, 2016
The use of biodegradable food packaging plants could provide an alternative to plastic packaging,... more The use of biodegradable food packaging plants could provide an alternative to plastic packaging, particularly for some traditional foods. This work aims to investigate the species of plant leaves used as food packaging in the food crafts in West Africa through their domestication. Data on different species of plant leaves have been collected through a survey among 8 municipalities in Benin using tools and methods based on focus groups. The criteria to select priority species for domestication have been identified from the pair wise comparison of plant species identified in each locality, using standard method. A larval toxicity test was performed on the main species to ensure their safety for domestication. A total of 43 plant species belonging to 29 families have been identified. From them, about 20% was domesticated and 80% were grown naturally. A total of 21.5% of natural species are in the way to be domesticated from which ten (10) have been identified as priority, non-toxic wi...
European Journal of Medicinal Plants, 2021
Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat... more Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract whil...
British Biotechnology Journal, 2015
The use of plants in traditional medicine become very common nowadays throughout the world and in... more The use of plants in traditional medicine become very common nowadays throughout the world and in developing countries in particular. The current study was carried out aiming to compare the chemical features of three vegetables (Vernonia amygdalina, Crateva adansonii and Sesamum radiatum) mostly used for human consumption and traditional medicine to treat bacterial diarrhoea in Benin. These vegetables were selected among the 27 species obtained after ethno-botanical investigations in southern Benin. Therefore, the major chemical groups contained in these vegetables were detected by solubility assays together with coloration and precipitation reactions. Furthermore, cytotoxicity of the plant extracts was assessed on shrimp larvae (Artemia salina). The results showed that gallic tannins, flavonoids, alkaloids, mucilages, coumarins and reducing compounds were detected in all samples (100%) followed by cathechic tannins (66.66%) and saponins (33.33%). The study also revealed that none of the studied vegetables is cytotoxic (LC 50 > 0.01 mg/ml). This study was conducted in the perspective of establishing innovations capable of assisting to; manufacture Improved Traditional Medicines for the treatment of bacterial diarrhoea in the future. Besides, it pointed out the presence of secondary metabolites and the cytotoxicity of these vegetables interesting for further antibacterial, antidiarrheal and pharmacological studies.
The comparative study of physico-chemical characteristics of Terminalia catappa L. kernel oils ex... more The comparative study of physico-chemical characteristics of Terminalia catappa L. kernel oils extracted by two methods has been done. The oil yields were 28.13 % and 61.78 % respectively for traditional and hexane methods and the fatty acid profiles showed palmitic acid (40.79 % and 40.03 % respectively) oleic acid (25.55 % and 26.09 % respectively), linoleic acid (26.72 % and 26.64 % respectively) and stearic acid (4.35 % and 4.49 % respectively) as major components. The oils extracted by the two ways showed similar physico-chemical properties, good calorific values and non-toxicity against Artemia salina L. Oil obtained by traditional method exhibited more antioxidant capacity (1.40) than the hexane one (0.15). This traditional method helps to extract 45 % of the total oil. It gives oil free of organic solvent, with good physico-chemical properties that could be useful as edible oil and for industrial applications.
Vigna comosa Baker (V. comosa) is a plant used by Beninese Oueme department's population for the ... more Vigna comosa Baker (V. comosa) is a plant used by Beninese Oueme department's population for the treatment of diabetes without any scientific study showing its effectiveness. The objective of this study is to investigate the effects of the extracts of V. comosaon hyperglycemic rabbits by Oral Glucose Tolerance Test (OGTT) and on hepatic glucose liberation. Phytochemical screening revealed that the plant contains alkaloids, tannins, flavonoids, mucilage, triterpenoids, steroids, reducing compounds, saponins, oses and holosides. Cytotoxity test showed that those extracts were free of toxicity. The extracts showed anti-hyperglycemic activities doses and time depending. The effective doses are 500 mg/kg for the aqueous extract and 1000 mg/kg for the ethanolic extract. The extracts are effective as compared with glibenclamide (reference product). Moreover, the ex vivo test conducted on the liver revealed that V. comosa aqueous extract inhibits the hepatic glucose liberation and 500 mg/mL is the most effective dose. The results of this study account for the plant under consideration of its traditional usage in the treatment of diabetes.