L. Solti | University of Veterinary Medicine, Budapest (original) (raw)

Papers by L. Solti

Theriogenology, 2003

Exposure to certain mycotoxins has been proved to contribute to fertility problems in pigs. Altho... more Exposure to certain mycotoxins has been proved to contribute to fertility problems in pigs. Although ochratoxin A (OA) is one of the most common naturally occurring mycotoxins, there is little data concerning the possible effects of this toxin on sperm quality of boars.

Reproduction in Domestic Animals, 1998

Three studies were carried out to monitor or induce ovarian activity and sexual behaviour in maid... more Three studies were carried out to monitor or induce ovarian activity and sexual behaviour in maiden, barren and postpartum mares during the anovulatory season. In the first study maiden and barren mares were followed up by regular blood sampling. In 39% of anoestrus mares the anovulatory season was started after December 1. Mares that stopped cycling before December 1 had significantly longer anovulatory period (P<O.OOl) and resumed cyclic ovarian activity one month later (P<O.Ol) than mares that stopped cycling after December 1. The first ovulation was not preceded by overt oestrus in most of the cases (64.7%). In the second study 38 mares, foaling in the first three month of the year, were monitored. Cyclic ovarian activity resumed more than 20 days after parturition in 11 mares (28.9%, between 22 and 11 1 days). Only 52.6% of the animals showed heat before the first postpartum ovulation. The follicular phase after the luteolysis of the first corpus luteum was longer than normal (>lodays, between 22 and 64 days) in 10 mares (43.5%). In the third study 44 mares in spring transition were treated with Altrenogest for 10 days. In 8 treated mares a GnRHchallenge (40pg Buserelin iv.) was carried out before treatment. Despite the same inclusion criteria used for all mares, in case of 11 mares (25%) the treatment was not successful and the ovulation occurred more than 20 days after the end of treatment (between 29 and 70 days). The result of the GnRH-stimulation gave an explanation for this difference. Mares that were treated successfully with Altrenogest had a higher maximum LH-response and area under the curve indicating higher LH-content of the pituitary before treatment.

Reproduction, Fertility and Development, 2006

The polscope uses novel electrooptical hardware and digital processing to image macromolecular st... more The polscope uses novel electrooptical hardware and digital processing to image macromolecular structures in cells on the basis of their birefringence (Sato et al . 1975 J. Cell Biol. 76, 501-517). Imaging of the spindle in living oocytes with the polscope is based on birefringence of an inherent physical property of the microtubules. De-polymerization of microtubules arrests meiosis and induces abnormal meiosis generating chromosomally abnormal oocytes which are not capable of being fertilized and developing. In this study we examined whether spindles in living mouse oocytes can be safely imaged/examined by the polscope and investigated the influence of sub-optimal temperature on spindle detection/visualization. For oocyte collection, 6-to 8-week-old CB6F1 female mice were superovulated with PMSG (10 IU, i.p.; Sigma, USA) and 46 to 48 h later they were injected with hCG (10 IU, i.p.; Sigma). Oocytes were collected 20 to 23 h after hCG treatment in MOPS buffered medium (G-MOPS"; Vitrolife, Sweden AB, Kungsbacka, Sweden) supplemented with human serum albumin (HAS"; Vitrolife). Cumulus cells were removed from the zonae pellucidae by exposure to a solution of 40 IU/mL hyaluronidase (HYASE"; Vitrolife). For spindle examination, each oocyte was placed in a 5-μL drop of G-MOPS medium, covered with oil (Ovoil; Vitrolife), and examined in a Delta T.C.O. dish with a specially coated glass bottom (Willco-Dish, Willco Wells, Amsterdam, The Netherlands). Oocytes were imaged by a Nikon Diaphot microscope with a video camera, objective lens, and controller, combined with a computerized imaging analysis system (CRI, Great Britain). For evaluation of the effect of sub-optimal temperature on the visualization/detection of the spindle, mouse oocytes were cultured for a short period of time at sub optimal temperatures (18 to -20°C/25 min and -10 to -15°C/2 min). After that, the oocytes were immediately checked for presence and condition of the meiotic spindle and re-checked after 30-min culture at 37°C with 6% CO , 5% O , 89% N and maximal humidity in air. There was no difference in the detect ability of the spindle in mouse oocytes with or without polar body (PB+: 21/24, 87%; PB-: 29/32, 90%). We found that the treatment with sub-optimal temperature has an effect to the spindle visualization. Immediately after treatment, the spindle could be detected in 80% of the oocytes (16/20). However, after 30-min culture, the meiotic spindle was detectable in only 25% of the treated oocytes (5/20; P< 0.05). Sub optimal temperature at first increased the birefringence of the meiotic spindle for a short period of time; then the spindle became completely undetectable. The increased sensitivity of the meiotic spindle to low temperature is one of the biggest obstacles to the oocyte cryopreservation. Our results provide further evidence that the spindle in mammalian oocytes is very sensitive to temperature fluctuations. The increase in the birefringence was the first sign that the structure of microtubules and other cytoskeletal factors forming the spindle had changed. Investigations are underway to learn more about the capability of the meiotic spindle to become reorganized.

Theriogenology, 2000

Among the many mammalian species that are threatened as the result of habitat destruction are num... more Among the many mammalian species that are threatened as the result of habitat destruction are numerous species of rare or little-known native livestock that possess features that render them ideally adapted to their environment. Because of the vital and valuable role many of these species play both to the ecology and economy of their native countries, attention is being directed towards initiating breeding programs that might insure their continued survival. This review introduces and highlights the importance of some of these indigenous species and outlines efforts currently underway to apply assisted reproductive technologies to their conservation. Q 1999 by Elsevier Science Inc.

Theriogenology, 1995

Direct transfer of cryopreserved-thawed embryos reduces time and expense involved in embryo trans... more Direct transfer of cryopreserved-thawed embryos reduces time and expense involved in embryo transfer. We compared 2 glycerol based techniques allowing direct transfer using IVP bovine embryos. The first was a conventional freezing method (Massip et al, Ann Med Vet, 131:515, 1987), while the second was a vitrification method (Rall, Anim Reprod Sci, 28237, 1992). Previous field trials with in vivo-produced embryos applying these techniques resulted in 60.5% (Touati et al, Proc 8th AETE Mtg, ~220, 1992) and 45% (Van Wagtendonk-de Leeuw et al, Theriogenology, 41:326, 1994) pregnancy rates, respectively.

Theriogenology, 1996

On the international embryo market the presence of zona pellucida is required to decrease the ris... more On the international embryo market the presence of zona pellucida is required to decrease the risk of disease transmission. Following embryo manipulations, the embryos are no longer in an intact zona. In those cases the optimal stage and method for cryopreservation needs to be determined and might include the hatched blastocyst stage, too. The technical possibility for freezing in vivo hatched blastocysts have been demonstrated by the very fist results in bovine (Wilmut and Rowson, Vet Ret 1973;92:686). We compared a conventional freezing (Massip et al., Ann Med Vet 1987;131:51.5) and a vitrification method (Rall, Anim Reprod Sci 1992;28:237) on in vitro produced bovine embryos (Vajta et al.,Theriogenology 1992;37:811).

Reproduction in Domestic Animals, 1999

Three experiments were conducted to determine whether fol! licular~uid "FF# enters the oviduct an... more Three experiments were conducted to determine whether fol! licular~uid "FF# enters the oviduct and plays any role in the transport of oocytes into the oviduct[ Experiment 0] Oestrus and ovulation were synchronized in cycling gilts "n 10# over a 04 day period of feeding Regumate þ and injections of 0999 IU pregnant mare|s serum gonadotrophin "PMSG# 13 h after the last Regumate þ feed and 499 IU human chorionic gon! adotrophin "hCG# 79 h after PMSG[ Ipsi!lateral aspiration of FF and salpingectomy "group 0\ n 6#\ aspiration of FF with! out salpingectomy "group 1\ n 6# or ligation of the oviduct between the ampulla and infundibulum "group 2\ n 6# was performed endoscopically prior to ovulation "23Ð25 h after hCG#[ Ipsi!lateral "group 1 and 2# and contra!lateral sal! pingectomy was carried out in all gilts post ovulation\ 31Ð33 h after hCG[ The oviducts were~ushed with 0 ml saline and the samples as well as the aspirated FF were analysed for pro! gesterone and estradiol by RIA methods[ In group 0 both pro! gesterone and estradiol concentrations did not di}er before and after ovulation[ Withdrawal of FF from the ipsi!lateral ovary by aspiration "group 1# or ligation of the oviduct "group 2# did not in~uence the steroid content within the oviducts[ Similarly low progesterone concentrations were measured in ipsi! and contra!lateral oviducts after ovulation "group 1] 9[18 2 9[06 versus 9[13 2 9[24 ng:ml and group 2] 9[11 2 9[08 versus 9[10 2 9[11 ng:ml#[ The high content of progesterone of FF "158[6 2 56[8 and 278[5 2 115[4 ng:ml in group 0 and 1\ respec! tively# was not re~ected in the oviductal~uid[ Experiment 1] In _ve gilts 9[95 ml 2 H!progesterone "29 999 dpm# were applied via a _ne 16 G injection needle into the largest three follicles of the ipsi!lateral ovary prior to ovulation "23Ð25 h after hCG#[ The oviducts were~ushed following ovario!salpingectomy 31Ð33 h after hCG[ All follicles had ovulated[ The oviductal~ushings and oviductal and ovarian tissue were analysed for labelled progesterone[ No di}erences were measured in the content of 2 H!progesterone of oviductal~ushings and of both oviductal and ovarian tissues between the ipsi!lateral injection and contra! lateral control sides[ The main part of the counts detected was within the range of background dpm values[ Only 1[3) of the initial counts were recovered from~uid and tissue samples[ Experiment 2] In a subsequent study FF was cautiously aspi! rated by endoscopy from follicles of the ipsi!lateral ovary 23Ð 25 h after hCG "n 01 gilts#[ Postovulatory "47 h after hCG#\ both oviducts were~ushed and the oocytes were recovered[ To test the in~uence of follicle puncture alone on the process of ovulation "n 7 gilts#\ the aspiration needle alone was pricked into the follicles of the ipsi!lateral ovary\ without any~uid aspiration [ Despite the cautious aspiration of FF from 78 follicles\ 15 oocytes were recovered together with the FF[ Eighty!six postovulatory follicles were observed on the ipsi! lateral ovary[ Out of 46 oocytes able to reach the oviduct\ 18 oocytes were~ushed from the oviduct "49[3 2 17[0)#[ From U[ S[ Copyright Clearance Center Code Statement] 9825Ð5657:88:2394Ð9312,03[99:9 the contra!lateral control oviduct 60 oocytes out of 80 ovu! lations "58[9 2 22[8)# were recaptured[ Puncture of follicles without aspiration did not in~uence ovulation compared with the control "recovery rate 57[1 and 68[5)\ respectively#[ Results indicate "0# on the basis of the low progesterone level within the oviductal~uid that only a small amount of FF seems to reach the oviduct at ovulation\ and "1# FF does not appear to be a compulsory carrier of the porcine oocyte at ovulation[ Inhalt Untersuchungen zur Rolle der Follikel~u Ãssigkeit beim Transfer von Oozyten des Schweines in den Eileiter bei der Ovulation In drei Experimenten wurde untersucht ob Follikel~u Ã ssigkeit "FF# in den Eileiter gelangt und ob sie eine Bedeutung beim Transport der Oozyten in den Eileiter hat[ Experiment 0] O Ýstrus und Ovulation wurden bei Jungsauen "n 10# mittels 04!ta Ãgi! ger Fu Ã tterung von Regumate þ und Injektionen von 0[999 IE PMSG 13 h nach der letzten Regumate þ !Fu Ã tterung sowie 499 IE hCG 79 h nach PMSG synchronisiert[ Eine ipsi!laterale FF! Aspiration und Salpingektomie "Gruppe 0\ n 6#\ die Aspiration der FF ohne Salpingektomie "Gruppe 1\ n 6# oder eine Ligation des Eileiters zwischen Ampulle und Infundibulum "Gruppe 2\ n 6# wurde endoskopisch ante ovulationem "23Ð 25 h nach hCG# durchgefu Ã hrt[ Die ipsi!laterale "Gruppe 1 und 2# und kontra!laterale Salpingektomie erfolgte bei allen Tieren post ovulationem "31Ð33 h nach hCG#[ Die Eileiter wurden mit 0 ml physiologischer Kochsalzlo Ã sung gespu Ã lt[ In diesen Proben sowie in der aspirierten FF wurden die Progesteron!und Estradiol!Konzentrationen radioimmunologisch bestimmt[ Gruppe 0 zeigte keine Unterschiede in den Steroidkonzentratio! nen vor und nach der Ovulation[ Ein Entzug der FF vom ipsi!lateralen Ovar durch Aspiration "Gruppe 1# oder Ligation "Gruppe 2# hatte keinen Ein~u) auf den Steroidgehalt im Ei! leiter[ Gleiche geringe Progesteronkonzentrationen wurden in den ipsi!und kontra!lateralen Eileitern nach der Ovulation gemessen "Gruppe 1] 9[18 2 9[06 vs[ 9[13 2 9[24 ng:ml^Gruppe 2] 9[11 2 9[08 vs[ 9[10 2 9[11 ng:ml#[ Der hohe Progesteronge! halt in der FF "158[6 2 56[8 ng:ml und 278[5 2 115[4 ng:ml in den Gruppen 0 und 1# spiegelte sich nicht in der Ei! leiterspu Ã l~u Ã ssigkeit wider[ Experiment 1] Bei 4 Jungsauen wur! den 9[95 ml 2 H!Progesteron "29[999 dpm# mittels einer feinen 16 G Injektionskanu Ã le in die drei gro Ã )ten Follikel des ipsi! lateralen Ovars ante ovulationem "23Ð25 h nach hCG# injiziert[ Die Eileiter wurden nach einer Ovariosalpingektomie 31Ð33 h nach hCG gespu Ã lt[ Alle Follikel hatten ovuliert[ Die Ei! leiter~u Ã ssigkeit sowie das Eileiter! und Ovargewebe wurde auf das markierte Progesteron untersucht[ Im Vergleich der ipsi! lateralen Injektions!und der kontra!lateralen Kontrollseite wurde im 2 H!Progesterongehalt in der Eileiterspu Ã l~u Ã ssigkeit 313 K!P Bru Ã ssow\ J Ra tky\ F Schneider\ H Torner\ W Kanitz and L Solti sowie im Eileiter! und Ovargewebe kein Unterschied ermittelt[ Der gro Ã )te Teil der ermittelten Za Ãhlimpulse lag im Bereich der Hintergrund!Impulsrate[ Nur 1\3) der applizierten Impulse wurden aus den Spu Ã l~u Ã ssigkeits!und Gewebeproben zuru Ã ckge! wonnen[ Experiment 2] Die FF wurde bei 01 Jungsauen ante ovulationem "23Ð25 h nach hCG# endoskopisch aus den Fol! likeln des ipsi!lateralen Ovars aspiriert[ Postovulatorisch "47 h nach hCG# wurden beide Eileiter gespu Ã lt und die Oozyten auf! gesucht[ Um nur den Ein~u) der Follikelpunktion auf den Ovulationsproze) zu pru Ã fen\ wurde bei weiteren 7 Tieren die Aspirationskanu Ã le nur in die Follikel des ipsi!lateralen Ovars eingestochen\ ohne die FF abzusaugen[ Trotz der vorsichtigen Aspiration der FF von 78 Follikeln wurden 15 Oozyten gemein! sam mit der FF abgesaugt[ Post ovulationem wurden 75 Ovu! lationen ermittelt[ Von den 46 Oozyten\ die bei der Ovu! lation in den Eileiter gelangen konnten\ wurden 18 aus dem Eileiter gewonnen "49[3 2 17[0)#[ Vom kontra!lateralen Kontrolleileiter wurden 60 Oozyten von 80 Ovulationen zuru Ã ckgewonnen "58[9 2 22[8)#[ Die alleinige Punktion der Follikel hatte keinen Ein~u) auf die Ovulation] Gewinnungs! rate 57[1) vs[ 68[5) "Kontrolle#[ Die Ergebnisse zeigen] "0# gemessen an den geringen Progesteronkonzentrationen in der Eileiter~u Ã ssigkeit gelangt bei der Ovulation nur eine unbedeu! tende Menge FF in den Eileiter\ und "1# die FF ist bei der Ovulation fu Ã r den Transport der Eizelle in den Eileiter nicht essentiell[

Molecular Reproduction and Development, 2000

The sensitivity of dog sperm cells for extracellular Ca 2ϩ /Ca 2ϩ -ionophore challenge was compar... more The sensitivity of dog sperm cells for extracellular Ca 2ϩ /Ca 2ϩ -ionophore challenge was compared to the detrimental effects of an optimized freeze/thawing protocol. Three sperm-rich fractions of ejaculates from 9 dogs were obtained, and one aliquot of each ejaculate was washed in a modified Tyrode's medium (HBT containing 0.1 mM Ca 2ϩ ), without (control sample) and with 2.5 M Ca 2ϩ -ionophore (induced sample) and incubated for 60 min at 38ЊC in humidified atmosphere. Another aliquot from the same semen fractions was diluted, washed in a Tris buffer, and packed into 0.5-ml straws with a Tris buffer containing 7.5 vol % glycerol. The samples were stored for 1 week in liquid nitrogen after a computer-driven three-step freeze protocol and subsequently thawed for 50 sec in a 37ЊC water bath and reconstituted into HBT. The acrosome integrity was determined using fluorescein-conjugated peanut agglutinin (PNA-FITC) as an acrosomal marker, while the vitality of the sperm cells was simultaneously assessed with the membrane impermeable DNA supravital stain ethidium homodimer 1 (EthD-1) using fluorescence microscopy and flow cytometry. The motility of frozen/thawed sperm samples was evaluated by microscopic as well as computerized motility analyses. Remarkably, the percentage sperm cells that underwent acrosome reactions induced by Ca 2ϩ -ionophore correlated very positively (r ϭ 0.93) with the amount of acrosome damage observed in cryopreserved sperm samples. Furthermore, the degree of cellular damage induced by Ca 2ϩ -ionophore treatment correlated very negatively (r ϭ Ϫ0.99) with the relative amount of sperm cells that remained motile after cryopreservation. Such clear correlations between Ca 2ϩ -ionophore induced acrosome reaction and motility parameters for frozen/thawed dog sperm cells were not found, suggesting that the generation of acrosome leakage and sperm immotility are two independent detrimental processes occurring during cryopreservation. From these results it can be concluded that Ca 2ϩ -ionophore treatment followed by simulta-neous determination PNA-FITC and EthD-1 staining can be used to predict the cryopreservability of ejaculates from individual dogs used as donors. Mol. Reprod. Dev.

Letters in Applied Microbiology, 1996

... picogram level A. Gyongyosi-Horvtith, 1. Barna-Vetro and L. Solti Agricultural Biotechnology ... more ... picogram level A. Gyongyosi-Horvtith, 1. Barna-Vetro and L. Solti Agricultural Biotechnology Center, Godollo, Hungary JIP/109: received 10 July 1995 and accepted 22 July 1995 A. GYGNGYOSI-HORVATH, I. BARNA-VETRO AND L. SOLTI. 1996. ...

Journal of Agricultural and Food Chemistry, 1996

A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been... more A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This ...

Animal Reproduction Science, 1999

Acta Veterinaria Hungarica, 2009

By decreasing the volume of the cryoprotective solution it is possible to increase dramatically t... more By decreasing the volume of the cryoprotective solution it is possible to increase dramatically the freezing speed and -at the same time -reduce the toxicity and osmotic side effects of cryoprotectants (CPA). The objective of our study was to vitrify Day-3 cleavage stage mouse embryos (n = 229) with the cryoloop technology using a new composition of vitrification media. Embryos were exposed to a 2-step loading of CPA, ethylene glycol (EG) and propylene glycol (PG), before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop, then they were rapidly submerged into liquid nitrogen. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Survival of embryos was based on morphological appearance after thawing and continued development to expanded blastocysts upon subsequent 48-hour culture. Embryos of the two control groups were either treated likewise except that they were not vitrified, or cultured in vitro without any treatment. Our data show that a high percentage of embryos survived (92.7%) vitrification in the mixture of EG and PG combined with cryoloop carrier and developed normally (89.1%) in vitro after thawing. To our knowledge this is the first report of the successful vitrification of cleavage stage mouse embryos using VitroLoop vitrification procedure.

Reproduction in Domestic Animals, 2003

The concentration of plasma progesterone was measured by ELISA, in serum and samples prepared wit... more The concentration of plasma progesterone was measured by ELISA, in serum and samples prepared with three different anticoagulant agents -namely ethylenediaminetetraacetic acid (EDTA), heparine and sodium fluoride oxalate potassium(NaFK). Forty clinically healthy bitches were selected based on the signs of pro-oestrus or oestrus. Values of progesterone concentration were significantly higher in serum than in EDTA-plasma (p < 0.0005); heparin-plasma (p < 0.05) and NaFK-plasma (p < 0.005). During prooestrus and oestrus until the time of ovulation, progesterone exhibited a conspicuous and statistically verified diurnal pattern (p < 0.05), its serum concentration being higher during 6.00-7.00 p.m. than 8.00-9.00 a.m. By the time of ovulation tendency of higher p.m. progesterone level reverses and from this point on the a.m. progesterone concentration is higher. The results of these experiments indicate that the concentration of canine progesterone assayed with ELISA may be affected by the time of collection and the method of preservation used.