Noureddine Bouras | UNIVERSITY OF GHARDAIA. ALGERIA (original) (raw)

Papers by Noureddine Bouras

Tunisian Journal of Plant Protection, 2021

Trials were conducted to test the hydrosols of 4 spontaneous plants on the date palm mite, Oligon... more Trials were conducted to test the hydrosols of 4 spontaneous plants on the date palm mite, Oligonychus afrasiaticus, a key pest of date palm in Algeria. Extracts from those plants (basil, harmel, colocynth and hyssop) tested against date palm mite showed promising results. The quantities of essential oils extracted from the 4 plants were extremely low, that is why only hydrosols were used. The results obtained change depending on the number of sprays and the reading time after treatment. The hyssop extract caused a high mortality rate of 91%, followed by colocynth and basil extracts with average mortality rates of 64% and 62%, respectively. The lowest mortality rate, 6%, occurred when applying harmel extract.

Letters in Applied Microbiology, 2019

A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis ... more A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis NRRL B-24137 in sorbic acid-amended medium. (2019) Letters in Applied Microbiology, 69 (4). 294-301.

Letters in Applied Microbiology, Sep 11, 2019

A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis ... more A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis NRRL B-24137 in sorbic acid-amended medium. (2019) Letters in Applied Microbiology, 69 (4). 294-301.

Annals of Microbiology, Feb 2, 2017

Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce ... more Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain's efficacy to reduce pure AFB1 content was studied in ISP2-medium. A f t e r a 4-d a y i n c u b a t i o n a t 3 0°C o n A F B 1supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9-97.6%). The three most efficient reducers (94.9-97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.

Letters in Applied Microbiology, Jan 4, 2019

Significance and Impact of the Study: Dithiolopyrrolone antibiotics, known for their strong antim... more Significance and Impact of the Study: Dithiolopyrrolone antibiotics, known for their strong antimicrobial activities, gained greater interest after the discovery of their antitumor properties. Depending on precursors added, Saccharothrix algeriensis NRRL B-24137 has the ability to produce several dithiolopyrrolones derivatives. Since biological activities of dithiolopyrrolones are related to their variable structure, discover of new natural analogues to be therapeutically explored remains a significant framework of research. In this study, a new dithiolopyrrolone derivative was purified from the fermentation broth of S. algeriensis NRRL B-24137. This new antibiotic, characterized as benzoyl-pyrrothine dithiolopyrrolone, was induced by adding cinnamic acid, as precursor, to a semi-synthetic medium.

Journal De Mycologie Medicale, Sep 1, 2016

Advanced Research in Life Sciences

Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of... more Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

Advanced Research in Life Sciences

Urinary tract infections (UTIs) are currently one of the most important medical practice visits. ... more Urinary tract infections (UTIs) are currently one of the most important medical practice visits. The spread of antibiotic-resistant bacteria in the community complicates the therapeutic management. The aim of this study is to investigate the bacteria associated with community acquired urinary tract infections (CA-UTIs) and the antibiotic resistance pattern of bacterial isolates during 2021 in Algiers, northern Algeria. A total of 548 urine samples from patients with CA-UTIs symptoms were analyzed. Pure bacterial isolates were identified using morphological and biochemical characteristics, and subjected to antibiogram evaluation using the disk diffusion method. A total 133 out of 548 patients (24.27%) had positive CA-UTIs, mainly affecting women (84.21%). A percentage of 87.22% of isolates were identified as Enterobacteriaceae, which were represented mostly by Escherichia coli (76 isolates), Klebsiella pneumoniae (13 isolates), and Proteus mirabilis (13 isolates). A number of 125 iso...

Advanced Research in Life Sciences

Kappa-carrageenase (EC 3.2.1.83) is a glycoside hydrolase family 16 (GH16) member that could spec... more Kappa-carrageenase (EC 3.2.1.83) is a glycoside hydrolase family 16 (GH16) member that could specifically hydrolyse kappa-carrageenans to kappa-carrageenan oligosaccharides. Kappa-carrageenase enzymes have attracted much interest due to their numerous potential applications in biomedical and physiological fields, bioethanol production, and textile industry. In the present study, physicochemical, secondary structure, structural properties including homology modeling, refinement, and model quality validation, and functional analyses of the kappacarrageenanse from Pseudomonas fluorescens using various bioinformatic tools were conducted. The protein was found to be stable and acidic in nature. Secondary structure prediction revealed that the presence of random coil is more dominated in the protein sequence followed by extended strand, α-helix, and β-turn. Protein-protein interaction prediction revealed ten potential functional partners. This bioinformatic characterization provides for t...

Periodica Polytechnica Chemical Engineering

In recent years, intensive research has been conducted on natural carotenoids extraction using se... more In recent years, intensive research has been conducted on natural carotenoids extraction using several processes. Conventional extraction methods require high amounts of solvents and a long extraction time. However, pressurized liquid extraction demonstrated to be an interesting method. The extraction efficiencies of pressurized liquid for the recovery of carotenoids, from the green and the orange biomasses of the microalga Dunaliella salina DunaDZ1, are described. Organic solvents were tested including ethanol, n-hexane, ethyl acetate and a mixture of n-hexane:ethanol (3:4). Moreover, three extraction temperatures were used (90, 120 and 150 °C) at constant pressure. Extraction efficiency and extracts characterization were conducted. Results have shown that temperature has a positive effect on extraction yield. HPLC characterization showed that β-carotene is the main carotenoid in the orange biomass, and lutein in the green biomass, with the presence of other minor carotenoids in bo...

Acta Universitatis Cibiniensis. Series E: Food Technology

Seven date seeds cultivars, Deglet Nour, Degla Baïdha, Fakht, Ghars, Hamraya, Tafezouin and Taker... more Seven date seeds cultivars, Deglet Nour, Degla Baïdha, Fakht, Ghars, Hamraya, Tafezouin and Takermost were provided from Algerian Sahara. Some of these Algerian date seeds were studied for the first time (Fakht, Hamraya and Takermost). The biochemical characterization of these date seeds were studied, including fatty acids and minerals profiles. The results showed that date seeds are a good source of carbohydrates and crude cellulose, with averages of 66.63 and 13.20%, respectively. They are also a good source of proteins, with the cultivar Deglet Nour seeds as the richest one (9.27 ± 0.03%). The main fatty acid for all date seeds oils is oleic acid except for Deglet Nour in which lauric acid is the main one. However, the studied date seed oils could be classified as oleic-lauric oil. Potassium was the highest mineral in all date seeds cultivars, with values ranging from 2700 to 2900 mg/Kg, followed by sodium and iron. These results indicate that date seeds have good nutritional val...

Theoretical and Applied Ecology

Current Trends in Natural Sciences

During a screening for the diversity of actinobacterial strains from Saharan soil samples collect... more During a screening for the diversity of actinobacterial strains from Saharan soil samples collected from Béchar region (Algeria), one strain designated KB-T3 was isolated by dilution technique on chitin-vitamins agar medium. The taxonomic position of this strain was determined by using a polyphasic approach. Morphological and chemical characteristics of the KB-T3 strain were consistent with those of the genus Thermobifida. The KB-T3 strain had a white aerial mycelium with dictomically branched sporophores carrying coccoid secluded spores. The substrate mycelium was pale yellow, sterile, and non-fragmented. The strain is characterized by the presence of meso-diaminopimelic acid in the cell wall, the galactose in whole-cell, and phosphatidylethanolamine in the cell membrane. The unique characteristic of this strain was its abundant growth with the absence of NaCl and in temperature‎ ranging from 40 to 65 °C, its capacity to decompose acetate, and its ability to use fructose, glucose a...

Kemija u industriji

This work is licensed under a Creative Commons Attribution 4.0 International License

The Journal of Microbiology, Biotechnology and Food Sciences, Jan 12, 2018

Lebanese Science Journal, 2019

n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saha... more n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saharan soils, an isolateof actinobacteria,designated Sg163, was selected for its interesting antimicrobial activity.The isolate wasidentified to the genus Streptosporangium by phenotypicand molecular criteria. The cultural and physiological characterizations as well as phylogenetic analysis indicated that the isolate was different from known members of the genus Streptosporangium. Analysis of the 16S rRNA sequence showed 97.09to 98.27% similarity with those of Streptosporangium type strains. The strainSg163 produced antifungal and antibacterial activities on several culture media. The highest antimicrobial activities were obtained in ISP2 medium. Three active products C1, C2 and C3 with both antifungal and antibacterial activities were isolated and purified by chromatographic methodswith C2, as the major compound. The data of the infraredspectroscopy andthe chemical revelations, suggested t...

Tunisian Journal of Plant Protection, 2021

Trials were conducted to test the hydrosols of 4 spontaneous plants on the date palm mite, Oligon... more Trials were conducted to test the hydrosols of 4 spontaneous plants on the date palm mite, Oligonychus afrasiaticus, a key pest of date palm in Algeria. Extracts from those plants (basil, harmel, colocynth and hyssop) tested against date palm mite showed promising results. The quantities of essential oils extracted from the 4 plants were extremely low, that is why only hydrosols were used. The results obtained change depending on the number of sprays and the reading time after treatment. The hyssop extract caused a high mortality rate of 91%, followed by colocynth and basil extracts with average mortality rates of 64% and 62%, respectively. The lowest mortality rate, 6%, occurred when applying harmel extract.

Letters in Applied Microbiology, 2019

A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis ... more A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis NRRL B-24137 in sorbic acid-amended medium. (2019) Letters in Applied Microbiology, 69 (4). 294-301.

Letters in Applied Microbiology, Sep 11, 2019

A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis ... more A new dithiolopyrrolone antibiotic triggered by a long fermentation of Saccharothrix algeriensis NRRL B-24137 in sorbic acid-amended medium. (2019) Letters in Applied Microbiology, 69 (4). 294-301.

Annals of Microbiology, Feb 2, 2017

Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce ... more Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain's efficacy to reduce pure AFB1 content was studied in ISP2-medium. A f t e r a 4-d a y i n c u b a t i o n a t 3 0°C o n A F B 1supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9-97.6%). The three most efficient reducers (94.9-97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.

Letters in Applied Microbiology, Jan 4, 2019

Significance and Impact of the Study: Dithiolopyrrolone antibiotics, known for their strong antim... more Significance and Impact of the Study: Dithiolopyrrolone antibiotics, known for their strong antimicrobial activities, gained greater interest after the discovery of their antitumor properties. Depending on precursors added, Saccharothrix algeriensis NRRL B-24137 has the ability to produce several dithiolopyrrolones derivatives. Since biological activities of dithiolopyrrolones are related to their variable structure, discover of new natural analogues to be therapeutically explored remains a significant framework of research. In this study, a new dithiolopyrrolone derivative was purified from the fermentation broth of S. algeriensis NRRL B-24137. This new antibiotic, characterized as benzoyl-pyrrothine dithiolopyrrolone, was induced by adding cinnamic acid, as precursor, to a semi-synthetic medium.

Journal De Mycologie Medicale, Sep 1, 2016

Advanced Research in Life Sciences

Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of... more Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

Advanced Research in Life Sciences

Urinary tract infections (UTIs) are currently one of the most important medical practice visits. ... more Urinary tract infections (UTIs) are currently one of the most important medical practice visits. The spread of antibiotic-resistant bacteria in the community complicates the therapeutic management. The aim of this study is to investigate the bacteria associated with community acquired urinary tract infections (CA-UTIs) and the antibiotic resistance pattern of bacterial isolates during 2021 in Algiers, northern Algeria. A total of 548 urine samples from patients with CA-UTIs symptoms were analyzed. Pure bacterial isolates were identified using morphological and biochemical characteristics, and subjected to antibiogram evaluation using the disk diffusion method. A total 133 out of 548 patients (24.27%) had positive CA-UTIs, mainly affecting women (84.21%). A percentage of 87.22% of isolates were identified as Enterobacteriaceae, which were represented mostly by Escherichia coli (76 isolates), Klebsiella pneumoniae (13 isolates), and Proteus mirabilis (13 isolates). A number of 125 iso...

Advanced Research in Life Sciences

Kappa-carrageenase (EC 3.2.1.83) is a glycoside hydrolase family 16 (GH16) member that could spec... more Kappa-carrageenase (EC 3.2.1.83) is a glycoside hydrolase family 16 (GH16) member that could specifically hydrolyse kappa-carrageenans to kappa-carrageenan oligosaccharides. Kappa-carrageenase enzymes have attracted much interest due to their numerous potential applications in biomedical and physiological fields, bioethanol production, and textile industry. In the present study, physicochemical, secondary structure, structural properties including homology modeling, refinement, and model quality validation, and functional analyses of the kappacarrageenanse from Pseudomonas fluorescens using various bioinformatic tools were conducted. The protein was found to be stable and acidic in nature. Secondary structure prediction revealed that the presence of random coil is more dominated in the protein sequence followed by extended strand, α-helix, and β-turn. Protein-protein interaction prediction revealed ten potential functional partners. This bioinformatic characterization provides for t...

Periodica Polytechnica Chemical Engineering

In recent years, intensive research has been conducted on natural carotenoids extraction using se... more In recent years, intensive research has been conducted on natural carotenoids extraction using several processes. Conventional extraction methods require high amounts of solvents and a long extraction time. However, pressurized liquid extraction demonstrated to be an interesting method. The extraction efficiencies of pressurized liquid for the recovery of carotenoids, from the green and the orange biomasses of the microalga Dunaliella salina DunaDZ1, are described. Organic solvents were tested including ethanol, n-hexane, ethyl acetate and a mixture of n-hexane:ethanol (3:4). Moreover, three extraction temperatures were used (90, 120 and 150 °C) at constant pressure. Extraction efficiency and extracts characterization were conducted. Results have shown that temperature has a positive effect on extraction yield. HPLC characterization showed that β-carotene is the main carotenoid in the orange biomass, and lutein in the green biomass, with the presence of other minor carotenoids in bo...

Acta Universitatis Cibiniensis. Series E: Food Technology

Seven date seeds cultivars, Deglet Nour, Degla Baïdha, Fakht, Ghars, Hamraya, Tafezouin and Taker... more Seven date seeds cultivars, Deglet Nour, Degla Baïdha, Fakht, Ghars, Hamraya, Tafezouin and Takermost were provided from Algerian Sahara. Some of these Algerian date seeds were studied for the first time (Fakht, Hamraya and Takermost). The biochemical characterization of these date seeds were studied, including fatty acids and minerals profiles. The results showed that date seeds are a good source of carbohydrates and crude cellulose, with averages of 66.63 and 13.20%, respectively. They are also a good source of proteins, with the cultivar Deglet Nour seeds as the richest one (9.27 ± 0.03%). The main fatty acid for all date seeds oils is oleic acid except for Deglet Nour in which lauric acid is the main one. However, the studied date seed oils could be classified as oleic-lauric oil. Potassium was the highest mineral in all date seeds cultivars, with values ranging from 2700 to 2900 mg/Kg, followed by sodium and iron. These results indicate that date seeds have good nutritional val...

Theoretical and Applied Ecology

Current Trends in Natural Sciences

During a screening for the diversity of actinobacterial strains from Saharan soil samples collect... more During a screening for the diversity of actinobacterial strains from Saharan soil samples collected from Béchar region (Algeria), one strain designated KB-T3 was isolated by dilution technique on chitin-vitamins agar medium. The taxonomic position of this strain was determined by using a polyphasic approach. Morphological and chemical characteristics of the KB-T3 strain were consistent with those of the genus Thermobifida. The KB-T3 strain had a white aerial mycelium with dictomically branched sporophores carrying coccoid secluded spores. The substrate mycelium was pale yellow, sterile, and non-fragmented. The strain is characterized by the presence of meso-diaminopimelic acid in the cell wall, the galactose in whole-cell, and phosphatidylethanolamine in the cell membrane. The unique characteristic of this strain was its abundant growth with the absence of NaCl and in temperature‎ ranging from 40 to 65 °C, its capacity to decompose acetate, and its ability to use fructose, glucose a...

Kemija u industriji

This work is licensed under a Creative Commons Attribution 4.0 International License

The Journal of Microbiology, Biotechnology and Food Sciences, Jan 12, 2018

Lebanese Science Journal, 2019

n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saha... more n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saharan soils, an isolateof actinobacteria,designated Sg163, was selected for its interesting antimicrobial activity.The isolate wasidentified to the genus Streptosporangium by phenotypicand molecular criteria. The cultural and physiological characterizations as well as phylogenetic analysis indicated that the isolate was different from known members of the genus Streptosporangium. Analysis of the 16S rRNA sequence showed 97.09to 98.27% similarity with those of Streptosporangium type strains. The strainSg163 produced antifungal and antibacterial activities on several culture media. The highest antimicrobial activities were obtained in ISP2 medium. Three active products C1, C2 and C3 with both antifungal and antibacterial activities were isolated and purified by chromatographic methodswith C2, as the major compound. The data of the infraredspectroscopy andthe chemical revelations, suggested t...