Tewfik Sahraoui | Université Oran 1 (original) (raw)

Papers by Tewfik Sahraoui

Viruses

Nasopharyngeal cancer (NPC) is a prevalent type of cancer that often takes the form of undifferen... more Nasopharyngeal cancer (NPC) is a prevalent type of cancer that often takes the form of undifferentiated carcinoma in the Maghreb region. It affects people of all ages. NPC diagnosis, mainly based on detecting Epstein-Barr virus (EBV), has not been well evaluated in North Africa. We compared the classical EBV serological tests using indirect immunofluorescence to the detection of EBV DNase antibodies by immunoblot in Algerian NPC patients. Significant variations were observed among different age groups of patients regarding the presence of VCA-IgA antibodies (0–14 and ≥30 years old, p < 0.0001; 15–19 and ≥30 years old, p < 0.01) and EA-IgA (0–14 and ≥30 years old, p < 0.01; 15–29 and ≥30 years old, p < 0.05). Differences were also noted in the titers of IgA anti-VCA and anti-EA antibodies across the three age groups. Some patients under the age of 30 with detectable IgG anti-VCA antibodies had undetectable IgA anti-VCA antibodies. These patients had a strong anti-DNase Ig...

Archives of Medical Science - Aging

Introduction: Since the onset of the SARS-CoV-2 pandemic in March 2019, scientists have been tryi... more Introduction: Since the onset of the SARS-CoV-2 pandemic in March 2019, scientists have been trying first to define the virus, the disease and even the factors that exacerbate the situation. Questions have arisen about the relationship between the patient's blood type, rhesus and the development of a COVID-19 infection. The purpose of this study is to determine whether or not there is a correlation between blood types, rhesus and COVID-19 disease, as well as its severity. Material and methods: We conducted a retrospective study of 7 months from August 2021 to February 2022, involving 59 patients with 33 male and 26 female subjects suspected of being infected with COVID-19, between the ages of 14 and 90. These patients were recruited from the Pasteur Institute "Oran Annex" and the Local Public Health Facility (EPSP BENI SAF). RT-qPCR techniques and blood grouping were used to determine the blood type of patients and whether or not they were infected with the SARS-CoV-2 virus. Patient information was retrieved from both facilities from medical records. Results: According to our results, 83% of patients tested positive for SARS-CoV-2 and 17% tested negative. The proportion of the O-group among those infected with SARS-CoV-2 (66%) was lower than in healthy patients (70%), while the proportion of the A-group (20%) was higher in negative patients than in positive patients (17%). The percentage of blood type B was identical in the affected and healthy groups (10%), while the AB group was present in affected patients (7%) and was completely absent in healthy patients. The p-value was 1 and this indicates that the link between blood type and COVID-19 infection is not significant. Conclusions: In light of our findings, there is a correlation between rhesus and the age of patients during the development of COVID-19 infection.

Pan African Medical Journal, 2021

Introduction: breast cancer is related to age. The young age remains a controversial issue as a p... more Introduction: breast cancer is related to age. The young age remains a controversial issue as a prognostic factor and have more aggressive clinical behavior with poor outcome. We aimed for the first time in Algeria to explore on a large cohort of patients the prevalence of the molecular phenotypes and to describe their clinical characteristics and survival. Methods: medical record of 1140 Algerian patients were analysed and categorized into three age groups: "young" when women were aged below 40 years; "middle-age" when women were aged from 41 to 54 years old and "elder" when women were over 54 years. Baseline categorical variables were analysed using the Chi-square test and survival curves were constructed using Kaplan Meir method. Results: the distribution of the various prognostic factors did not differ significativelly by age groups except for histological types, hormone receptors status and molecular phenotypes. Most patients were luminal A, indeed, young and intermediate age patients were most likely to be luminal A whereas the aged patients were triple negative with the highest mean DFS. Elsewhere young women are considered as human epidermal growth factor receptor 2 (HER2+) or triple negative molecular subtypes involving more rigorous therapeutic monitoring. The high rate of triple negative breast cancer in aged patients may due to genetic predispositions. Conclusion: this study sheds light on the histoclinical and molecular characteristics of breast cancer in young patients, which has a good prognosis than their older counterparts. Our results are therefore surprisingly different from what the literature suggests. A further study should understand this uncommon finding.

Japanese Journal of Cancer Research, 2000

Context: Breast cancer is the most common cause of cancer mortality among women worldwide. It is ... more Context: Breast cancer is the most common cause of cancer mortality among women worldwide. It is a heterogeneous disease partly responsible for treatment failure in luminal B patients. Deregulation of fibroblast growth factor signaling has been found and its therapeutic/prognostic value is explored. Aims: Most of the research has studied the FGFR1 gene while our study explored its protein expression by immunohistochemestry and examined the association with clinicopathological features, different molecular subtypes and survival. Subjects and Methods: Formalin-fixed and paraffin-embedded samples of invasive breast carcinomas were used to analyze FGFR1 expression. FGFR 1 was scored by percentage and intensity of cell cytoplasm staining, correlations were investigated and survival curves were constructed. Statistical Analysis Used: Chi-square test was used to assess the relationship between the marker expression and the clinicopathological characteristics. Overall specific survival curv...

AIM The objective of this study was to evaluate the expression of hypoxia-inducible transcription... more AIM The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. METHODS We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. RESULTS The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age ...

INTRODUCTION Luminal B breast cancer is associated with a poor prognosis and resistance to hormon... more INTRODUCTION Luminal B breast cancer is associated with a poor prognosis and resistance to hormone therapy. Studies have suggested that Ras-related protein 25 (RAB25), a member of Rab small GTPase family, is involved in breast cancer pathogenesis. Our aim in the present study is to analyze the association between RAB25 protein expression and clinical and pathological characteristics, and to investigate whether the expression of RAB25 was associated with a specific molecular subtype of breast cancer. MATERIALS AND METHODS A retrospective study was conducted regarding female patients diagnosed with breast cancer; clinicopathologic data was obtained from medical reports. RAB25 expression was evaluated by immunohistochemistry in 57 primary breast cancer samples. The results were correlated with clinicopathologic variables and different breast cancer molecular subtypes. RESULTS RAB25 expression was significantly associated with tumors expressing oestrogen receptor (P=0.03). A high signif...

Journal of Drug Delivery and Therapeutics

Objective: The aim of the present study is to describe the epidemiological, histopathological and... more Objective: The aim of the present study is to describe the epidemiological, histopathological and therapeutic profile of endometrial cancers in the region of Oran. Methods: We conducted a retrospective study by exploring the medical files of 25 female patients diagnosed of endometrial cancer and treated at the level of the EHU November 1, 1954’s medical oncology department in Oran during the period from January 2015 to December 2019. For data collection, we used a structured exploitation sheet to obtain necessary information. Variables were analyzed using SPSS Software Version 20.0. Results: The median age of patients was 59 years with extremes ranging from 42 to 83 years. More than 56% of our patients were over 50 years old, 40% of the patients were nulliparous and 80% postmenopausal. The average age of menarche was 14.09 ± 1.44 years with extremes ranging from 12 to 17 years. The indication for anatomopathological examination was dominated by metrorrhagia (80%). Histopathologicall...

Journal of Drug Delivery and Therapeutics

Objective : The aim of this study is to describe the sperm profile and eventually determine the m... more Objective : The aim of this study is to describe the sperm profile and eventually determine the main spermatic disturbances at the origin of male infertility in western Algeria. Methods: We conducted a cross-sectional study in 320 infertile male patients consulting at the Medically Assisted Procreation Unit (MAP) of Oran, in Western Algeria, in the end of exploring the results of their semen analyses and semen culture. Results: The results of semen analysis revealed that the main spermatic disruption in our study was asthenospermia because at the first hour after emission, 93% of our patients had less mobility than normal. Oligospermia was present in 37% of cases and azoospermia in 14% of cases. That is, a concentration of sperm below normal in 51% of cases. Results of the analysis of the sperm’s morphological characteristics revealed that only 16% of our patients had teratospermia. The semen culture revealed that the main pathogen found in our patients is Staphylococci in nearly 70...

Journal of Drug Delivery and Therapeutics

Objective : The aim of this study is to describe the sperm profile and eventually determine the m... more Objective : The aim of this study is to describe the sperm profile and eventually determine the main spermatic disturbances at the origin of male infertility in western Algeria. Methods: We conducted a cross-sectional study in 320 infertile male patients consulting at the Medically Assisted Procreation Unit (MAP) of Oran, in Western Algeria, in the end of exploring the results of their semen analyses and semen culture. Results: The results of semen analysis revealed that the main spermatic disruption in our study was asthenospermia because at the first hour after emission, 93% of our patients had less mobility than normal. Oligospermia was present in 37% of cases and azoospermia in 14% of cases. That is, a concentration of sperm below normal in 51% of cases. Results of the analysis of the sperm’s morphological characteristics revealed that only 16% of our patients had teratospermia. The semen culture revealed that the main pathogen found in our patients is Staphylococci in nearly 70...

Annales de Biologie Clinique

Annales de Biologie Clinique

The Gulf journal of oncology, 2016

The objective of this study was to evaluate the expression of hypoxia-inducible transcription fac... more The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age of 49 ± 1year. Clini...

The Gulf journal of oncology, 2016

The objective of this study was to evaluate the expression of hypoxia-inducible transcription fac... more The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age of 49 ± 1year. Clini...

The Gulf journal of oncology, 2017

Breast cancer is the most common cancer in women worldwide. Biology contributes to the early diag... more Breast cancer is the most common cancer in women worldwide. Biology contributes to the early diagnosis and monitoring of breast cancer with several categories of markers such as prognostic markers (ER, PR, HER2), proliferative markers (Ki67), and tumor markers such as CEA and CA 15-3. CEA can be detected at a high concentration in serum of patients with malignant tumors. The aim of our study was to evaluate the concentrations of CEA in serum of women with breast cancer and to verify the existence of a possible link between the average rates of CEA and SBR grade. Serum samples from 100 patients with breast cancer and 100 controls was recovered and examined with an AxSYM analyzer (Abbott Laboratories, USA) to measure CEA using Microparticle Enzyme Immunoassay (MEIA) technology. In our clinical study, the mean age of patients and controls were 52.7 and 50.3 years respectively. The results revealed an elevation in the CEA levels from patients with an average value of 16.61 ± 0.2 ng/ml. ...

Annales de biologie clinique, 2018

Breast cancer is the common malignancy that affects women worldwide, but conventional risk factor... more Breast cancer is the common malignancy that affects women worldwide, but conventional risk factors account for only a small proportion of these cases. A possible viral etiology for breast cancer has been proposed and Epstein-Barr virus (EBV) is a widely studied candidate virus. The objective of this study is to determine the association of EBV infection with infiltrating ductal carcinomas (IDC). This descriptive study was carried out in the laboratory of developmental biology and differentiation, from 2012 to 2014. Of 39 cases, we determined the clinicopathological characteristics of the population. Of the 23 cases of IDC, we implemented the techniques Elisa, immunohistochemistry and in situ hybridization. To determine the serological profile, overexpression of onco-proteins EBNA-1, HER2, the mitotic index Ki67 and detection of the presence of the viral genome. The mean age is 57.40±4, SBR II predominates with 70%, pN+ (27%), RE+ (58%), RP+ (52%), HER2 (81%), Luminal A (34%), Lumina...

Viruses

Nasopharyngeal cancer (NPC) is a prevalent type of cancer that often takes the form of undifferen... more Nasopharyngeal cancer (NPC) is a prevalent type of cancer that often takes the form of undifferentiated carcinoma in the Maghreb region. It affects people of all ages. NPC diagnosis, mainly based on detecting Epstein-Barr virus (EBV), has not been well evaluated in North Africa. We compared the classical EBV serological tests using indirect immunofluorescence to the detection of EBV DNase antibodies by immunoblot in Algerian NPC patients. Significant variations were observed among different age groups of patients regarding the presence of VCA-IgA antibodies (0–14 and ≥30 years old, p < 0.0001; 15–19 and ≥30 years old, p < 0.01) and EA-IgA (0–14 and ≥30 years old, p < 0.01; 15–29 and ≥30 years old, p < 0.05). Differences were also noted in the titers of IgA anti-VCA and anti-EA antibodies across the three age groups. Some patients under the age of 30 with detectable IgG anti-VCA antibodies had undetectable IgA anti-VCA antibodies. These patients had a strong anti-DNase Ig...

Archives of Medical Science - Aging

Introduction: Since the onset of the SARS-CoV-2 pandemic in March 2019, scientists have been tryi... more Introduction: Since the onset of the SARS-CoV-2 pandemic in March 2019, scientists have been trying first to define the virus, the disease and even the factors that exacerbate the situation. Questions have arisen about the relationship between the patient's blood type, rhesus and the development of a COVID-19 infection. The purpose of this study is to determine whether or not there is a correlation between blood types, rhesus and COVID-19 disease, as well as its severity. Material and methods: We conducted a retrospective study of 7 months from August 2021 to February 2022, involving 59 patients with 33 male and 26 female subjects suspected of being infected with COVID-19, between the ages of 14 and 90. These patients were recruited from the Pasteur Institute "Oran Annex" and the Local Public Health Facility (EPSP BENI SAF). RT-qPCR techniques and blood grouping were used to determine the blood type of patients and whether or not they were infected with the SARS-CoV-2 virus. Patient information was retrieved from both facilities from medical records. Results: According to our results, 83% of patients tested positive for SARS-CoV-2 and 17% tested negative. The proportion of the O-group among those infected with SARS-CoV-2 (66%) was lower than in healthy patients (70%), while the proportion of the A-group (20%) was higher in negative patients than in positive patients (17%). The percentage of blood type B was identical in the affected and healthy groups (10%), while the AB group was present in affected patients (7%) and was completely absent in healthy patients. The p-value was 1 and this indicates that the link between blood type and COVID-19 infection is not significant. Conclusions: In light of our findings, there is a correlation between rhesus and the age of patients during the development of COVID-19 infection.

Pan African Medical Journal, 2021

Introduction: breast cancer is related to age. The young age remains a controversial issue as a p... more Introduction: breast cancer is related to age. The young age remains a controversial issue as a prognostic factor and have more aggressive clinical behavior with poor outcome. We aimed for the first time in Algeria to explore on a large cohort of patients the prevalence of the molecular phenotypes and to describe their clinical characteristics and survival. Methods: medical record of 1140 Algerian patients were analysed and categorized into three age groups: "young" when women were aged below 40 years; "middle-age" when women were aged from 41 to 54 years old and "elder" when women were over 54 years. Baseline categorical variables were analysed using the Chi-square test and survival curves were constructed using Kaplan Meir method. Results: the distribution of the various prognostic factors did not differ significativelly by age groups except for histological types, hormone receptors status and molecular phenotypes. Most patients were luminal A, indeed, young and intermediate age patients were most likely to be luminal A whereas the aged patients were triple negative with the highest mean DFS. Elsewhere young women are considered as human epidermal growth factor receptor 2 (HER2+) or triple negative molecular subtypes involving more rigorous therapeutic monitoring. The high rate of triple negative breast cancer in aged patients may due to genetic predispositions. Conclusion: this study sheds light on the histoclinical and molecular characteristics of breast cancer in young patients, which has a good prognosis than their older counterparts. Our results are therefore surprisingly different from what the literature suggests. A further study should understand this uncommon finding.

Japanese Journal of Cancer Research, 2000

Context: Breast cancer is the most common cause of cancer mortality among women worldwide. It is ... more Context: Breast cancer is the most common cause of cancer mortality among women worldwide. It is a heterogeneous disease partly responsible for treatment failure in luminal B patients. Deregulation of fibroblast growth factor signaling has been found and its therapeutic/prognostic value is explored. Aims: Most of the research has studied the FGFR1 gene while our study explored its protein expression by immunohistochemestry and examined the association with clinicopathological features, different molecular subtypes and survival. Subjects and Methods: Formalin-fixed and paraffin-embedded samples of invasive breast carcinomas were used to analyze FGFR1 expression. FGFR 1 was scored by percentage and intensity of cell cytoplasm staining, correlations were investigated and survival curves were constructed. Statistical Analysis Used: Chi-square test was used to assess the relationship between the marker expression and the clinicopathological characteristics. Overall specific survival curv...

AIM The objective of this study was to evaluate the expression of hypoxia-inducible transcription... more AIM The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. METHODS We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. RESULTS The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age ...

INTRODUCTION Luminal B breast cancer is associated with a poor prognosis and resistance to hormon... more INTRODUCTION Luminal B breast cancer is associated with a poor prognosis and resistance to hormone therapy. Studies have suggested that Ras-related protein 25 (RAB25), a member of Rab small GTPase family, is involved in breast cancer pathogenesis. Our aim in the present study is to analyze the association between RAB25 protein expression and clinical and pathological characteristics, and to investigate whether the expression of RAB25 was associated with a specific molecular subtype of breast cancer. MATERIALS AND METHODS A retrospective study was conducted regarding female patients diagnosed with breast cancer; clinicopathologic data was obtained from medical reports. RAB25 expression was evaluated by immunohistochemistry in 57 primary breast cancer samples. The results were correlated with clinicopathologic variables and different breast cancer molecular subtypes. RESULTS RAB25 expression was significantly associated with tumors expressing oestrogen receptor (P=0.03). A high signif...

Journal of Drug Delivery and Therapeutics

Objective: The aim of the present study is to describe the epidemiological, histopathological and... more Objective: The aim of the present study is to describe the epidemiological, histopathological and therapeutic profile of endometrial cancers in the region of Oran. Methods: We conducted a retrospective study by exploring the medical files of 25 female patients diagnosed of endometrial cancer and treated at the level of the EHU November 1, 1954’s medical oncology department in Oran during the period from January 2015 to December 2019. For data collection, we used a structured exploitation sheet to obtain necessary information. Variables were analyzed using SPSS Software Version 20.0. Results: The median age of patients was 59 years with extremes ranging from 42 to 83 years. More than 56% of our patients were over 50 years old, 40% of the patients were nulliparous and 80% postmenopausal. The average age of menarche was 14.09 ± 1.44 years with extremes ranging from 12 to 17 years. The indication for anatomopathological examination was dominated by metrorrhagia (80%). Histopathologicall...

Journal of Drug Delivery and Therapeutics

Objective : The aim of this study is to describe the sperm profile and eventually determine the m... more Objective : The aim of this study is to describe the sperm profile and eventually determine the main spermatic disturbances at the origin of male infertility in western Algeria. Methods: We conducted a cross-sectional study in 320 infertile male patients consulting at the Medically Assisted Procreation Unit (MAP) of Oran, in Western Algeria, in the end of exploring the results of their semen analyses and semen culture. Results: The results of semen analysis revealed that the main spermatic disruption in our study was asthenospermia because at the first hour after emission, 93% of our patients had less mobility than normal. Oligospermia was present in 37% of cases and azoospermia in 14% of cases. That is, a concentration of sperm below normal in 51% of cases. Results of the analysis of the sperm’s morphological characteristics revealed that only 16% of our patients had teratospermia. The semen culture revealed that the main pathogen found in our patients is Staphylococci in nearly 70...

Journal of Drug Delivery and Therapeutics

Objective : The aim of this study is to describe the sperm profile and eventually determine the m... more Objective : The aim of this study is to describe the sperm profile and eventually determine the main spermatic disturbances at the origin of male infertility in western Algeria. Methods: We conducted a cross-sectional study in 320 infertile male patients consulting at the Medically Assisted Procreation Unit (MAP) of Oran, in Western Algeria, in the end of exploring the results of their semen analyses and semen culture. Results: The results of semen analysis revealed that the main spermatic disruption in our study was asthenospermia because at the first hour after emission, 93% of our patients had less mobility than normal. Oligospermia was present in 37% of cases and azoospermia in 14% of cases. That is, a concentration of sperm below normal in 51% of cases. Results of the analysis of the sperm’s morphological characteristics revealed that only 16% of our patients had teratospermia. The semen culture revealed that the main pathogen found in our patients is Staphylococci in nearly 70...

Annales de Biologie Clinique

Annales de Biologie Clinique

The Gulf journal of oncology, 2016

The objective of this study was to evaluate the expression of hypoxia-inducible transcription fac... more The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age of 49 ± 1year. Clini...

The Gulf journal of oncology, 2016

The objective of this study was to evaluate the expression of hypoxia-inducible transcription fac... more The objective of this study was to evaluate the expression of hypoxia-inducible transcription factor 1α (HIF1α) protein profile among women with breast cancer in a population of western Algerian and to correlate the intensity of this expression with prognosis histological grade Scarff Bloom and Richardson (SBR) of the disease and the age of the patients. We used a kit provided by Life Science Inc (UScnk) for a sandwich enzyme immunoassay for the quantitative measurement of in vitro HIF1α in plasma in 44 patients and 44 controls in a population of western Algerian. Then we looked for the correlation between the intensity of the expression of HIF1α and prognosis histological grade SBR and the age of the patients studied. The results indicate high levels of HIF1α at SBRIII grade with an average value of 12.26 ± 0.5 ng/ml. The average age of patients is around 50 ± 1year. In healthy subjects the mean concentration of 1.88 ± 0.1 ng/ml represents HIF1α with a mean age of 49 ± 1year. Clini...

The Gulf journal of oncology, 2017

Breast cancer is the most common cancer in women worldwide. Biology contributes to the early diag... more Breast cancer is the most common cancer in women worldwide. Biology contributes to the early diagnosis and monitoring of breast cancer with several categories of markers such as prognostic markers (ER, PR, HER2), proliferative markers (Ki67), and tumor markers such as CEA and CA 15-3. CEA can be detected at a high concentration in serum of patients with malignant tumors. The aim of our study was to evaluate the concentrations of CEA in serum of women with breast cancer and to verify the existence of a possible link between the average rates of CEA and SBR grade. Serum samples from 100 patients with breast cancer and 100 controls was recovered and examined with an AxSYM analyzer (Abbott Laboratories, USA) to measure CEA using Microparticle Enzyme Immunoassay (MEIA) technology. In our clinical study, the mean age of patients and controls were 52.7 and 50.3 years respectively. The results revealed an elevation in the CEA levels from patients with an average value of 16.61 ± 0.2 ng/ml. ...

Annales de biologie clinique, 2018

Breast cancer is the common malignancy that affects women worldwide, but conventional risk factor... more Breast cancer is the common malignancy that affects women worldwide, but conventional risk factors account for only a small proportion of these cases. A possible viral etiology for breast cancer has been proposed and Epstein-Barr virus (EBV) is a widely studied candidate virus. The objective of this study is to determine the association of EBV infection with infiltrating ductal carcinomas (IDC). This descriptive study was carried out in the laboratory of developmental biology and differentiation, from 2012 to 2014. Of 39 cases, we determined the clinicopathological characteristics of the population. Of the 23 cases of IDC, we implemented the techniques Elisa, immunohistochemistry and in situ hybridization. To determine the serological profile, overexpression of onco-proteins EBNA-1, HER2, the mitotic index Ki67 and detection of the presence of the viral genome. The mean age is 57.40±4, SBR II predominates with 70%, pN+ (27%), RE+ (58%), RP+ (52%), HER2 (81%), Luminal A (34%), Lumina...