F Emele | Nnamdi Azikiwe University (original) (raw)
Papers by F Emele
Journal of Infection and Public Health, 2015
Background: Antibiotic-resistant Staphylococcus aureus including methicillinresistant strains (MR... more Background: Antibiotic-resistant Staphylococcus aureus including methicillinresistant strains (MRSA) are a major concern in densely populated urban areas. Initial studies of S. aureus in Nigeria indicated existence of antibiotic-resistant S. aureus strains in clinical and community settings. Methods: 73 biological samples (40 throat, 23 nasal, 10 wound) were collected from patients and healthcare workers in three populations in Nigeria:
West African Journal of Medicine
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
West African Journal of Medicine
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).
African Journal of Clinical and Experimental Microbiology, Nov 15, 2017
Background: Antimicrobial resistance among Candida species is an intense public health concern. T... more Background: Antimicrobial resistance among Candida species is an intense public health concern. The aim of the study was to determine the antifungal susceptibility pattern and test for cure of Candida species among women of child bearing age who visited the General Hospital Onitsha, Nigeria with symptoms suggestive of Vulvovaginal Candidiasis (VVC). Materials and Methods: Eight hundred and seventy six female patients participated in the study of which high vaginal swabs were collected and evaluated mycological by standard microbiological methods: microscopic examination and culture using sabouraud dextrose agar (SDA). Susceptibility of isolates to 4 antifungal agents was tested using agar dilution method. Clinicomycological evaluation was also performed among the patients. Result: Higher minimum inhibitory concentration (MIC) to azole antifungals was observed predominantly among non-albicans Candida species increasingly involved in VVC. The rate of mycological resolution was higher than symptomatic relief at 2 weeks after treatment with antifungal drug. Conclusion: Efficacious treatment of VVC requires an adequate knowledge of the causative agents and more importantly the antimicrobial to which they exhibit high susceptibility.
Acta Paediat, 2007
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
Diagnostic Microbiology and Infectious Disease, Jul 31, 2001
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Chlamydia infection is a common sexually transmitted infection (STI) in humans caused by the bact... more Chlamydia infection is a common sexually transmitted infection (STI) in humans caused by the bacterium Chlamydia trachomatis . This study assessed the seroprevalence of Chlamydia trachomatis antibodies among students in two tertiary institutions in Anambra State, Nigeria. It was a comparative cross-sectional survey using enzyme immunoassay kits for the determination of IgG class antibodies, while the multistage sampling technique was used in the selection of participants for the study. The overall prevalence of Chlamydia trachomatis antibodies observed in this survey was 14.3%, which was higher among students from the University (21.6%) than their counterparts from the College of Education (7.4%) (χ 2 =5.89, df=1p<0.015). Factors found to have significant effect on the seroprevalence of Chlamydia trachomatis in both institutions were: prior sexual exposure (p<0.05); unprotected sexual intercourse in the last one year (p<0.05); multiple sexual partners in the last one year ...
West African journal of medicine, 1999
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
Sexually transmitted infections (STI) occur worldwide with more than 340 million cases occurring ... more Sexually transmitted infections (STI) occur worldwide with more than 340 million cases occurring annually in adults aged 15-49 years. The aim of the study is to determine the prevalence and co-existence of Chlamydia trachomatis and Herpes simplex virus (HSV) antibodies among students in a tertiary institution in Anambra State, Nigeria. This is a cross sectional descriptive study of the co–existence of Chlamydia trachomatis and HSV antibodies among students in a tertiary institution in Anambra State Nigeria carried out using enzyme immunoassay kits for determination of IgG class antibodies. The seroprevalence of HSV antibodies, (27.3%), was slightly higher than that of Chlamydia trachomatis, (21.6%) among the students. Also more of the students, (18.2%) were positive to HSV antibodies only, when compared to 12.5% of them that were positive to Chlamydia trachomatis antibodies only, while 9.1% of them were seropositive to both organisms. The antibody titres to both organisms were found...
International Journal of Infectious Diseases, 2020
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).
Diagnostic Microbiology and Infectious Disease, Jul 31, 2001
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Acta Paediatrica, Aug 1, 2000
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
West African journal of medicine
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
Acta paediatrica (Oslo, Norway : 1992), 1998
The prevalence and mode of spread of gonococcal infections was studied among prepubertal children... more The prevalence and mode of spread of gonococcal infections was studied among prepubertal children in Nigeria. Of 16 children with symptoms suggestive of sexually transmissible diseases (STD), 9 (56%) had gonorrhoea, while no causative organism was found in 7. The majority (7; 78%) of the gonococcal isolates produced penicillinase. Three of the cases were by child-to-child transmission, with female peers as the initiators. Prepubertal children should no longer be ignored as propagators of STD.
Mycoses, 2008
Two plant products, Euphorbia hirta leaves and fruits of Musa sapienturn, were evaluated as princ... more Two plant products, Euphorbia hirta leaves and fruits of Musa sapienturn, were evaluated as principal ingredients for selective cultivation of fungi. Sapienturn glucose agar supported the growth of both dermatophytic, yeast-like, and saprophytic fungi; growth on this medium compared favourably with growth on Sabouraud glucose agar, a standard mycological medium. Sporulation and pigment formation were stronger on sapienturn glucose agar than on Sabouraud glucose agar, although fungal growth on the latter was more luxuriant. Addition of Euphorbia extract to mycological media remarkably enhanced fungal growth on the media, and concomitantly suppressed bacterial growth to a similar extent as did antibiotics. The results of this study suggest that Euphorbia sapienturn glucose agar can safely be recommended as a cheap and efficient medium for routine isolation of fungi in both clinical and general mycological studies. Zusammenfassung. Blatter von Euphorbia hirta und Fruchte von Musa sapienturn, wurden in ihrer Eigenschaft als Bestandteile von Selektivmedien fur Pilze bewertet. Sapienturn-Glucose-Agar forderte das Wachstum sowohl von Dermatophyten als auch von Hefen und Schimmelpilzen; die Wachstumsintensitat auf diesem Medium war gut vergleichbar mit dem auf dem mykologischen Standardmedium Sabouraud-Glucose-Agar. Spo
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Acta Paediatrica, 2000
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
Mycoses, 2008
Tinea capitis continues to be a common problem of infancy and childhood, particularly among those... more Tinea capitis continues to be a common problem of infancy and childhood, particularly among those living in unhygienic crowded conditions. The prevalence and aetiology of tinea capitis have not been well defined in Anambra state of Nigeria - hence the need to undertake this study. A total of 47,723 primary school children residing in different regions of the State was screened for tinea capitis between 2002 and 2005. Of this number, 4498 (9.4%) had tinea capitis. The highest prevalence of the disease occurred in the Southern region of the state (12.6%). Schools in urban areas recorded lower prevalence of the disease. Male : female prevalence ratio of 1.6 : 1 was recorded. Tinea capitis occurred significantly more in children below 10 years of age than in those above this age (chi(2 )= 3.178; P < 0.10). Of 502 isolates recovered in culture, Microsporum audouinii was the most prevalent (42%), followed by M. ferrugineum (17%) and Trichophyton mentagrophytes (16%). Four of the samples revealed spore head of Aspergillus spp. in direct sample microscopy. A total of 29 (6%) isolates was atypical forms. The majority (91%) of children with the lesion came from families of three or more children, living in crowded apartments. Results showed that tinea capitis remains an important public health problem in Anambra state of Nigeria.
West African journal of medicine
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).
Journal of Infection and Public Health, 2015
Background: Antibiotic-resistant Staphylococcus aureus including methicillinresistant strains (MR... more Background: Antibiotic-resistant Staphylococcus aureus including methicillinresistant strains (MRSA) are a major concern in densely populated urban areas. Initial studies of S. aureus in Nigeria indicated existence of antibiotic-resistant S. aureus strains in clinical and community settings. Methods: 73 biological samples (40 throat, 23 nasal, 10 wound) were collected from patients and healthcare workers in three populations in Nigeria:
West African Journal of Medicine
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
West African Journal of Medicine
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).
African Journal of Clinical and Experimental Microbiology, Nov 15, 2017
Background: Antimicrobial resistance among Candida species is an intense public health concern. T... more Background: Antimicrobial resistance among Candida species is an intense public health concern. The aim of the study was to determine the antifungal susceptibility pattern and test for cure of Candida species among women of child bearing age who visited the General Hospital Onitsha, Nigeria with symptoms suggestive of Vulvovaginal Candidiasis (VVC). Materials and Methods: Eight hundred and seventy six female patients participated in the study of which high vaginal swabs were collected and evaluated mycological by standard microbiological methods: microscopic examination and culture using sabouraud dextrose agar (SDA). Susceptibility of isolates to 4 antifungal agents was tested using agar dilution method. Clinicomycological evaluation was also performed among the patients. Result: Higher minimum inhibitory concentration (MIC) to azole antifungals was observed predominantly among non-albicans Candida species increasingly involved in VVC. The rate of mycological resolution was higher than symptomatic relief at 2 weeks after treatment with antifungal drug. Conclusion: Efficacious treatment of VVC requires an adequate knowledge of the causative agents and more importantly the antimicrobial to which they exhibit high susceptibility.
Acta Paediat, 2007
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
Diagnostic Microbiology and Infectious Disease, Jul 31, 2001
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Chlamydia infection is a common sexually transmitted infection (STI) in humans caused by the bact... more Chlamydia infection is a common sexually transmitted infection (STI) in humans caused by the bacterium Chlamydia trachomatis . This study assessed the seroprevalence of Chlamydia trachomatis antibodies among students in two tertiary institutions in Anambra State, Nigeria. It was a comparative cross-sectional survey using enzyme immunoassay kits for the determination of IgG class antibodies, while the multistage sampling technique was used in the selection of participants for the study. The overall prevalence of Chlamydia trachomatis antibodies observed in this survey was 14.3%, which was higher among students from the University (21.6%) than their counterparts from the College of Education (7.4%) (χ 2 =5.89, df=1p<0.015). Factors found to have significant effect on the seroprevalence of Chlamydia trachomatis in both institutions were: prior sexual exposure (p<0.05); unprotected sexual intercourse in the last one year (p<0.05); multiple sexual partners in the last one year ...
West African journal of medicine, 1999
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
Sexually transmitted infections (STI) occur worldwide with more than 340 million cases occurring ... more Sexually transmitted infections (STI) occur worldwide with more than 340 million cases occurring annually in adults aged 15-49 years. The aim of the study is to determine the prevalence and co-existence of Chlamydia trachomatis and Herpes simplex virus (HSV) antibodies among students in a tertiary institution in Anambra State, Nigeria. This is a cross sectional descriptive study of the co–existence of Chlamydia trachomatis and HSV antibodies among students in a tertiary institution in Anambra State Nigeria carried out using enzyme immunoassay kits for determination of IgG class antibodies. The seroprevalence of HSV antibodies, (27.3%), was slightly higher than that of Chlamydia trachomatis, (21.6%) among the students. Also more of the students, (18.2%) were positive to HSV antibodies only, when compared to 12.5% of them that were positive to Chlamydia trachomatis antibodies only, while 9.1% of them were seropositive to both organisms. The antibody titres to both organisms were found...
International Journal of Infectious Diseases, 2020
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).
Diagnostic Microbiology and Infectious Disease, Jul 31, 2001
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Acta Paediatrica, Aug 1, 2000
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
West African journal of medicine
Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibili... more Bacteria associated with wound infection in Ekpoma, Nigeria, and their antimicrobial susceptibility profile was investigated by standard microbiological methods, using hospital as well as non-hospital patients. Of 40 patients seen, 25 (62.5%) were males, while the rest were females. Those aged 30 years and above accounted for 63% of the patients, and post-operative sepsis was the most frequently encountered wound infection. Of the organisms encountered, Staphylococcus aureus was the most frequently occurring organism (39%), followed by coliform bacilli (24%), which was the most prevalent organism (44%) in post-operative sepsis. Twenty-one percent of the isolates were Pseudomonas aeruginosa. The majority of the bacterial isolates from the infected wounds were susceptible to Gentamicin, as follows: 92% of the Staph. aureus, 100% of Streptococcus faecalis, Escherichia coli and Pseud. aeruginosa, and 75% of the coliform bacilli. It is suggested that gentamicin, in combination with metro...
Acta paediatrica (Oslo, Norway : 1992), 1998
The prevalence and mode of spread of gonococcal infections was studied among prepubertal children... more The prevalence and mode of spread of gonococcal infections was studied among prepubertal children in Nigeria. Of 16 children with symptoms suggestive of sexually transmissible diseases (STD), 9 (56%) had gonorrhoea, while no causative organism was found in 7. The majority (7; 78%) of the gonococcal isolates produced penicillinase. Three of the cases were by child-to-child transmission, with female peers as the initiators. Prepubertal children should no longer be ignored as propagators of STD.
Mycoses, 2008
Two plant products, Euphorbia hirta leaves and fruits of Musa sapienturn, were evaluated as princ... more Two plant products, Euphorbia hirta leaves and fruits of Musa sapienturn, were evaluated as principal ingredients for selective cultivation of fungi. Sapienturn glucose agar supported the growth of both dermatophytic, yeast-like, and saprophytic fungi; growth on this medium compared favourably with growth on Sabouraud glucose agar, a standard mycological medium. Sporulation and pigment formation were stronger on sapienturn glucose agar than on Sabouraud glucose agar, although fungal growth on the latter was more luxuriant. Addition of Euphorbia extract to mycological media remarkably enhanced fungal growth on the media, and concomitantly suppressed bacterial growth to a similar extent as did antibiotics. The results of this study suggest that Euphorbia sapienturn glucose agar can safely be recommended as a cheap and efficient medium for routine isolation of fungi in both clinical and general mycological studies. Zusammenfassung. Blatter von Euphorbia hirta und Fruchte von Musa sapienturn, wurden in ihrer Eigenschaft als Bestandteile von Selektivmedien fur Pilze bewertet. Sapienturn-Glucose-Agar forderte das Wachstum sowohl von Dermatophyten als auch von Hefen und Schimmelpilzen; die Wachstumsintensitat auf diesem Medium war gut vergleichbar mit dem auf dem mykologischen Standardmedium Sabouraud-Glucose-Agar. Spo
Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Envir... more Twenty-five Aeromonas hydrophila isolates from different sources (Food, 13; Clinical, 6 and Environmental, 6) were studied for the mode of production of Amylase and rapid iodometric detection of the enzyme in vitro. All twenty-five of the isolates produced the enzyme constitutively at 37°C. Amylase producing ability was not dependent on the source of isolation of Aeromonas (F ϭ 0.1069; p Ͼ 0.05). Using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 10(40%) of the isolates within 30 min, in 22(88%) within 60 min and in all (25 or 100%) within 90 min. The rapid detection of Aeromonas amylase will, no doubt, be of great value in routine diagnostic microbiology.
Acta Paediatrica, 2000
Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman D... more Etiologic agents of meningitis were prospectively investigated among patients admitted to Usman Danfodio University Teaching Hospital, Sokoto. Of 1097 cerebrospinal fluid (CSF) samples submitted to the microbiology laboratory from various wards of the hospital, 289 (26%) were microscopically, culturally and/or serologically proven to be bacterial meningitis. The etiologic spectrum was as follows: Neisseria meningitidis (61%), Streptococcus pneumoniae (18%), Haemophilus influenzae (10%), Staphylococcus aureus (6%), Coliform bacilli (3%), Escherichia coli (0.7%), Mycobacterium tuberculosis (0.7%), Listeria monocytogenes (0.4%), Flavobacterium meningosepticum (0.4%) and Pseudomonas putrifasciens (0.4%). Bacterial meningitis was most prevalent (195 or 68%) among children aged 1-9 y, while adults and neonates were least affected. Coliform bacilli caused five of eight neonatal cases. Males were more frequently affected than females (chi2 = 12.50; p < 0.05). Culture and microscopy were comparatively less efficient than the search for bacterial antigens, especially in the diagnosis of Haemophilus meningitis. Antimicrobial susceptibility of N. meningitidis to ampicillin and benzyl penicillin reduced progressively over the years (F = 406.98; p < 0.001). Nineteen (11%) of the isolates (5 Meningococci, 7 Staph. aureus, 1 Haem. influenza and 6 others) showed simultaneous resistance to chloramphenicol, ampicillin and benzyl penicillin.
Mycoses, 2008
Tinea capitis continues to be a common problem of infancy and childhood, particularly among those... more Tinea capitis continues to be a common problem of infancy and childhood, particularly among those living in unhygienic crowded conditions. The prevalence and aetiology of tinea capitis have not been well defined in Anambra state of Nigeria - hence the need to undertake this study. A total of 47,723 primary school children residing in different regions of the State was screened for tinea capitis between 2002 and 2005. Of this number, 4498 (9.4%) had tinea capitis. The highest prevalence of the disease occurred in the Southern region of the state (12.6%). Schools in urban areas recorded lower prevalence of the disease. Male : female prevalence ratio of 1.6 : 1 was recorded. Tinea capitis occurred significantly more in children below 10 years of age than in those above this age (chi(2 )= 3.178; P < 0.10). Of 502 isolates recovered in culture, Microsporum audouinii was the most prevalent (42%), followed by M. ferrugineum (17%) and Trichophyton mentagrophytes (16%). Four of the samples revealed spore head of Aspergillus spp. in direct sample microscopy. A total of 29 (6%) isolates was atypical forms. The majority (91%) of children with the lesion came from families of three or more children, living in crowded apartments. Results showed that tinea capitis remains an important public health problem in Anambra state of Nigeria.
West African journal of medicine
A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole a... more A controlled triple open clinical study on three vaginal tablets--Econazole nitrate, Miconazole and Nystatin, was carried out. Seventy-five patients, aged between 18 and 45 years, presenting with mycologically proven cases of vaginal candidiasis, met certain set criteria and were admitted into the study. Twenty-five patients were randomly assigned to each of the three treatment groups. Results of the study showed that at the 4th week after treatment, Econazole was comparable in antifungal action to Miconazole (x2 = 0.2128; p > 0.05) but significantly more antifungal than Nystatin (x2 = 8.8540; P < 0.05), although the overall clinicomycological assessment of the drugs showed no significant difference in their ratings (F = 21.34; P > 0.05).