Marcelo César Murguía | Universidad Nacional del Litoral (original) (raw)

Papers by Marcelo César Murguía

Research paper thumbnail of Liquid chromatography tandem-mass spectrometry (LC-MS/MS) and dried blood spot sampling applied to pharmacokinetics studies in animals: Correlation of classic and block design

Laboratory Animals, Oct 23, 2017

A relative bioavailability study (RBA) of two phenytoin (PHT) formulations was conducted in rabbi... more A relative bioavailability study (RBA) of two phenytoin (PHT) formulations was conducted in rabbits, in order to compare the results obtained from different matrices (plasma and blood from dried blood spot (DBS) sampling) and different experimental designs (classic and block). The method was developed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in plasma and blood samples. The different sample preparation techniques, plasma protein precipitation and DBS, were validated according to international requirements. The analytical method was validated with ranges 0.20–50.80 and 0.12–20.32 µg ml−1, r > 0.999 for plasma and blood, respectively. Accuracy and precision were within acceptance criteria for bioanalytical assay validation (< 15 for bias and CV% and < 20 for limit of quantification (LOQ)). PHT showed long-term stability, both for plasma and blood, and under refrigerated and room temperature conditions. Haematocrit values were measured during the validation process and RBA study. Finally, the pharmacokinetic parameters (Cmax, Tmax and AUC0–t) obtained from the RBA study were tested. Results were highly comparable for matrices and experimental designs. A matrix correlation higher than 0.975 and a ratio of (PHT blood) = 1.158 (PHT plasma) were obtained. The results obtained herein show that the use of classic experimental design and DBS sampling for animal pharmacokinetic studies should be encouraged as they could help to prevent the use of a large number of animals and also animal euthanasia. Finally, the combination of DBS sampling with LC-MS/MS technology showed to be an excellent tool not only for therapeutic drug monitoring but also for RBA studies.

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Research paper thumbnail of ChemInform Abstract: New Fluorinated Pyrazole and Uracil Derivatives: Synthesis and Biological Activity

ChemInform, 2009

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

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Research paper thumbnail of Anticarsia gemmatalis nuclear polyhedrosis virus replication in serum-free and serum-reduced insect cell cultures

Journal of Biotechnology, 1993

In order to develop a financially feasible process to produce Anticarsia gemmatalis Nuclear Polyh... more In order to develop a financially feasible process to produce Anticarsia gemmatalis Nuclear Polyhedrosis virus in cell culture, we developed a lipidic supplement to replace fetal calf serum in insect cell culture media. The supplement, prepared with an extract of lipids from hen egg yolk, allowed us to reduce the contents of serum in the culture medium from 10% to 1%. IPLB-Sf-21 cells could be kept along consecutive passages in serum-reduced medium. The replication of AgNPV in HEYLE-supplemented cultures was evaluated. Extracellular virions production was the same as in FCS-supplemented-cultures, but the production level of polyhedral inclusion bodies was significantly lowered in HEYLE-supplemented cultures. The reduced production of PIBs is related to a premature releasing of non-occluded particles as well as to a reduced synthesis of polyhedrin protein.

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Research paper thumbnail of <i>N</i>-Acetylated Gemini Surfactants: Synthesis, Surface-Active Properties, Antifungal Activity, and Ecotoxicity Bioassays

Advances in Chemical Engineering and Science, 2015

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Research paper thumbnail of Synthesis, Surface-Active Properties, and Antimicrobial Activities of New Double-Chain Gemini Surfactants

Journal of Oleo Science, 2008

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Research paper thumbnail of Production and Sensory Evaluation of Novel Cheeses Made with Prebiotic Substances: Inulin and Oligofructose

Food and Nutrition Sciences, 2015

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Research paper thumbnail of Design, synthesis, and insecticidal activity of novel neonicotinoid derivatives containing N-oxalyl groups

Arkivoc, 2009

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Research paper thumbnail of New Fluorinated Pyrazol and Uracil Derivatives: Synthesis and Biological Activity

ACS Symposium Series, 2009

... of a 3-trifluoromethyl-5-arylpyrazole derivative with methyl iodide under basic conditions af... more ... of a 3-trifluoromethyl-5-arylpyrazole derivative with methyl iodide under basic conditions afforded a 30:70 mixture of two regioisomers in favor of the unwanted 3-furyl derivative, and that if the methylation reaction was carried out with dimethyl sulfate, the ratio increased to 4:96. ...

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Research paper thumbnail of Synthesis and Properties of Cleavable Quaternary Ammonium Compounds

Journal of Oleo Science, 2021

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Research paper thumbnail of One-pot synthesis and insecticidal activity of 5-amino-1-aryl- 1H-pyrazole-4-carbonitriles

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Research paper thumbnail of Regio-specific synthesis of new 1-( tert -butyl)-1 H -pyrazolecarboxamide derivatives

Tetrahedron Letters, 2017

Regio-specific and non-regiospecific condensation reactions on 1,3-dicarbonyl compounds rendered ... more Regio-specific and non-regiospecific condensation reactions on 1,3-dicarbonyl compounds rendered 1,3,5-trisubstituted pyrazoles. Herein, the control of regio-specificity was a significant improvement in pyrazole research. A high yield acylation of poorly nucleophilic aryl amines, which resulted in mono- or diacylated products depending on the reaction conditions, is described. As a result, a library of potentially bioactive compounds was obtained.

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Research paper thumbnail of Bioequivalence Evaluation of Two Brands of Ketoprofen 50 Mg Capsules (Flogofin®&Profenid®) In Healthy Latin American Volunteers

Journal of Bioequivalence & Bioavailability, 2015

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Research paper thumbnail of Cationic gemini compounds with antifungal activity and wood preservation potentiality

Journal of Industrial and Engineering Chemistry, 2019

Abstract Three gemini compounds were synthesized and characterized in the search of an alternativ... more Abstract Three gemini compounds were synthesized and characterized in the search of an alternative eco-friendly wood preservative. Synthesis was carried out from N,N-dimethylamines and 2-O-acetyl-1,3- dichloropropane as linker molecule. All the synthesized molecules were adequately purified and characterized by TLC, GC, FT-IR and NMR. Their tensioactive properties were determined. All three efficiently lowered the surface tension in aqueous solutions with critical micelle concentrations ranging from 0.53 to 1.34 mM. Their antifungal activities were evaluated from a broth dilution test against four yeasts and two filamentous fungi and were compared to commercially available references: Fluconazole and TCMTB (2-thiocyanomethylthiobenzothiazole). All gemini compounds displayed good antifungal activity; particularly, the gemini derived from N,N-dimethyldodecylamine, with even better performance than Fluconazole against Saccharomyces cerevisiae, Aspergillus niger and Alternaria sp., and than TCMTB against the last one. The environmental impact of the gemini compound that showed the highest antifungal activity was analyzed through ecotoxicity tests, using Chlorella vulgaris and Daphnia magna as model organisms. It was several orders of magnitude less toxic than TCMTB. Finally, wood preservation efficacy studies were carried out on pine (Pinus ponderosa) samples. Results demonstrated accomplished resistance against brown rot fungi, comparable to a widespread commercial hydrosoluble preservative.

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Research paper thumbnail of Design and Synthesis of N-acetylated Gemini Surfactants for Use as Leather Preservatives during the Chrome-tanning Process

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Research paper thumbnail of Saliva as a Noninvasive Biological Sample to Compare Bioavailability of Phenytoin Formulations by LC-MS/MS

International Journal of Pharmaceutical Sciences and Research

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Research paper thumbnail of Saliva as a Noninvasive Biological Sample to Compare Bioavailability of Phenytoin Formulations by LC-MS/MS

International Journal of Pharmaceutical Sciences and Research

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Research paper thumbnail of Synthesis and surface‐active properties of novel cleavable gemini surfactants

Journal of Surfactants and Detergents

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Research paper thumbnail of Synthesis and Properties of New Cleavable Cationic Surfactants Containing Carbonate Groups

Journal of Surfactants and Detergents

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Research paper thumbnail of Emerging Trends in Engineering Research and Technology Vol. 9

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Research paper thumbnail of Bioequivalence Evaluation of Two Brands of Ketoprofen 50 Mg Capsules (Flogofin®&Profenid®) In Healthy Latin American Volunteers

Journal of Bioequivalence & Bioavailability

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Research paper thumbnail of Liquid chromatography tandem-mass spectrometry (LC-MS/MS) and dried blood spot sampling applied to pharmacokinetics studies in animals: Correlation of classic and block design

Laboratory Animals, Oct 23, 2017

A relative bioavailability study (RBA) of two phenytoin (PHT) formulations was conducted in rabbi... more A relative bioavailability study (RBA) of two phenytoin (PHT) formulations was conducted in rabbits, in order to compare the results obtained from different matrices (plasma and blood from dried blood spot (DBS) sampling) and different experimental designs (classic and block). The method was developed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in plasma and blood samples. The different sample preparation techniques, plasma protein precipitation and DBS, were validated according to international requirements. The analytical method was validated with ranges 0.20–50.80 and 0.12–20.32 µg ml−1, r &gt; 0.999 for plasma and blood, respectively. Accuracy and precision were within acceptance criteria for bioanalytical assay validation (&lt; 15 for bias and CV% and &lt; 20 for limit of quantification (LOQ)). PHT showed long-term stability, both for plasma and blood, and under refrigerated and room temperature conditions. Haematocrit values were measured during the validation process and RBA study. Finally, the pharmacokinetic parameters (Cmax, Tmax and AUC0–t) obtained from the RBA study were tested. Results were highly comparable for matrices and experimental designs. A matrix correlation higher than 0.975 and a ratio of (PHT blood) = 1.158 (PHT plasma) were obtained. The results obtained herein show that the use of classic experimental design and DBS sampling for animal pharmacokinetic studies should be encouraged as they could help to prevent the use of a large number of animals and also animal euthanasia. Finally, the combination of DBS sampling with LC-MS/MS technology showed to be an excellent tool not only for therapeutic drug monitoring but also for RBA studies.

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Research paper thumbnail of ChemInform Abstract: New Fluorinated Pyrazole and Uracil Derivatives: Synthesis and Biological Activity

ChemInform, 2009

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

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Research paper thumbnail of Anticarsia gemmatalis nuclear polyhedrosis virus replication in serum-free and serum-reduced insect cell cultures

Journal of Biotechnology, 1993

In order to develop a financially feasible process to produce Anticarsia gemmatalis Nuclear Polyh... more In order to develop a financially feasible process to produce Anticarsia gemmatalis Nuclear Polyhedrosis virus in cell culture, we developed a lipidic supplement to replace fetal calf serum in insect cell culture media. The supplement, prepared with an extract of lipids from hen egg yolk, allowed us to reduce the contents of serum in the culture medium from 10% to 1%. IPLB-Sf-21 cells could be kept along consecutive passages in serum-reduced medium. The replication of AgNPV in HEYLE-supplemented cultures was evaluated. Extracellular virions production was the same as in FCS-supplemented-cultures, but the production level of polyhedral inclusion bodies was significantly lowered in HEYLE-supplemented cultures. The reduced production of PIBs is related to a premature releasing of non-occluded particles as well as to a reduced synthesis of polyhedrin protein.

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Research paper thumbnail of <i>N</i>-Acetylated Gemini Surfactants: Synthesis, Surface-Active Properties, Antifungal Activity, and Ecotoxicity Bioassays

Advances in Chemical Engineering and Science, 2015

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Research paper thumbnail of Synthesis, Surface-Active Properties, and Antimicrobial Activities of New Double-Chain Gemini Surfactants

Journal of Oleo Science, 2008

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Research paper thumbnail of Production and Sensory Evaluation of Novel Cheeses Made with Prebiotic Substances: Inulin and Oligofructose

Food and Nutrition Sciences, 2015

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Research paper thumbnail of Design, synthesis, and insecticidal activity of novel neonicotinoid derivatives containing N-oxalyl groups

Arkivoc, 2009

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Research paper thumbnail of New Fluorinated Pyrazol and Uracil Derivatives: Synthesis and Biological Activity

ACS Symposium Series, 2009

... of a 3-trifluoromethyl-5-arylpyrazole derivative with methyl iodide under basic conditions af... more ... of a 3-trifluoromethyl-5-arylpyrazole derivative with methyl iodide under basic conditions afforded a 30:70 mixture of two regioisomers in favor of the unwanted 3-furyl derivative, and that if the methylation reaction was carried out with dimethyl sulfate, the ratio increased to 4:96. ...

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Research paper thumbnail of Synthesis and Properties of Cleavable Quaternary Ammonium Compounds

Journal of Oleo Science, 2021

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Research paper thumbnail of One-pot synthesis and insecticidal activity of 5-amino-1-aryl- 1H-pyrazole-4-carbonitriles

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Research paper thumbnail of Regio-specific synthesis of new 1-( tert -butyl)-1 H -pyrazolecarboxamide derivatives

Tetrahedron Letters, 2017

Regio-specific and non-regiospecific condensation reactions on 1,3-dicarbonyl compounds rendered ... more Regio-specific and non-regiospecific condensation reactions on 1,3-dicarbonyl compounds rendered 1,3,5-trisubstituted pyrazoles. Herein, the control of regio-specificity was a significant improvement in pyrazole research. A high yield acylation of poorly nucleophilic aryl amines, which resulted in mono- or diacylated products depending on the reaction conditions, is described. As a result, a library of potentially bioactive compounds was obtained.

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Research paper thumbnail of Bioequivalence Evaluation of Two Brands of Ketoprofen 50 Mg Capsules (Flogofin®&Profenid®) In Healthy Latin American Volunteers

Journal of Bioequivalence & Bioavailability, 2015

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Research paper thumbnail of Cationic gemini compounds with antifungal activity and wood preservation potentiality

Journal of Industrial and Engineering Chemistry, 2019

Abstract Three gemini compounds were synthesized and characterized in the search of an alternativ... more Abstract Three gemini compounds were synthesized and characterized in the search of an alternative eco-friendly wood preservative. Synthesis was carried out from N,N-dimethylamines and 2-O-acetyl-1,3- dichloropropane as linker molecule. All the synthesized molecules were adequately purified and characterized by TLC, GC, FT-IR and NMR. Their tensioactive properties were determined. All three efficiently lowered the surface tension in aqueous solutions with critical micelle concentrations ranging from 0.53 to 1.34 mM. Their antifungal activities were evaluated from a broth dilution test against four yeasts and two filamentous fungi and were compared to commercially available references: Fluconazole and TCMTB (2-thiocyanomethylthiobenzothiazole). All gemini compounds displayed good antifungal activity; particularly, the gemini derived from N,N-dimethyldodecylamine, with even better performance than Fluconazole against Saccharomyces cerevisiae, Aspergillus niger and Alternaria sp., and than TCMTB against the last one. The environmental impact of the gemini compound that showed the highest antifungal activity was analyzed through ecotoxicity tests, using Chlorella vulgaris and Daphnia magna as model organisms. It was several orders of magnitude less toxic than TCMTB. Finally, wood preservation efficacy studies were carried out on pine (Pinus ponderosa) samples. Results demonstrated accomplished resistance against brown rot fungi, comparable to a widespread commercial hydrosoluble preservative.

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Research paper thumbnail of Design and Synthesis of N-acetylated Gemini Surfactants for Use as Leather Preservatives during the Chrome-tanning Process

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Research paper thumbnail of Saliva as a Noninvasive Biological Sample to Compare Bioavailability of Phenytoin Formulations by LC-MS/MS

International Journal of Pharmaceutical Sciences and Research

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Research paper thumbnail of Saliva as a Noninvasive Biological Sample to Compare Bioavailability of Phenytoin Formulations by LC-MS/MS

International Journal of Pharmaceutical Sciences and Research

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Research paper thumbnail of Synthesis and surface‐active properties of novel cleavable gemini surfactants

Journal of Surfactants and Detergents

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Research paper thumbnail of Synthesis and Properties of New Cleavable Cationic Surfactants Containing Carbonate Groups

Journal of Surfactants and Detergents

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Research paper thumbnail of Emerging Trends in Engineering Research and Technology Vol. 9

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Research paper thumbnail of Bioequivalence Evaluation of Two Brands of Ketoprofen 50 Mg Capsules (Flogofin®&Profenid®) In Healthy Latin American Volunteers

Journal of Bioequivalence & Bioavailability

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