Guillermo Schinella | Universidad Nacional de La Plata (original) (raw)

Papers by Guillermo Schinella

Phytotherapy in the management of diabetes and hypertension, 2020

Olive leaves are used in Mediterranean folk medicine for the treatment of diabetes, hypertension ... more Olive leaves are used in Mediterranean folk medicine for the treatment of diabetes, hypertension and hypercholesterolemia since Ancient times. In the last decades, different authors have studied its chemical composition and ratified its pharmacological properties both in vitro and in vivo and, more recently, clinical trials focusing on its effects on diabetes and hypertension have been developed. In this chapter, information about recent studies on olive leaf are compiled, including its effects on the specific subject of this chapter, but also on other potential pharmacological effects.Fil: Rios, José Luis. Universidad de Valencia; EspañaFil: Andujar, Isabel. Universidad de Valencia; España. Universidad Europea de Valencia; EspañaFil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Universidad Arturo Jauretche; ArgentinaFil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentin

Tercera Época, Apr 1, 2016

Revista de fitoterapia, 2016

Tercera Época, Oct 1, 2011

Pathophysiology, Dec 1, 2018

The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not c... more The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not completely understood, not even the pathways that determine clinical manifestations associated to systemic protein deposition in organs such as liver, kidney and heart. About twenty natural variants of apoA-I were described as inducing amyloidosis, but the mechanisms driving their aggregation and deposition are still unclear. We previously identified that the mutant Gly26Arg but not Lys107-0 induced the release of cytokines and reactive oxygen species from cultured RAW 264.7 murine macrophages, suggesting that part of the pathogenic pathway could elicit of an inflammatory signal. In this work we gained deep insight into this mechanism and determined that Gly26Arg induced a specific pro-inflammatory cascade involving activation of NF-B and its translocation into the nucleus. These findings suggest that some but not all apoA-I natural variants might promote a pro-oxidant microenvironment which could in turn result in oxidative processing of the variants into a misfolded conformation.

Tercera Época, Nov 1, 2014

Journal of Pharmacy and Pharmacology, Mar 1, 2002

The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extrac... more The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) and ethyl phenylpropiolate in the mouse ear, by serotonin and phospholipase A 2 (PLA 2) in the mouse paw, on chronic in ammation induced by repeated application of TPA in the mouse ear and on the delayed-type hypersensitivity induced by sheep red blood cells suggest that said anti-in ammatory activity is due to the effects of compounds expressed via a corticoid-like mechanism. In addition, the antioxidant activity of the extracts seems to be implicated in this anti-in ammatory activity, as the former inhibits enzymatic and non-enzymatic lipid peroxidation and has free-radical scavenger properties. We conclude that the antiin ammatory activity of Helichrysum italicum can be explained by multiple effects, including in ammatory enzyme inhibition, free-radical scavenging activity and corticoid-like effects.

Planta Medica, Jul 1, 2015

Journal of Applied Physiology, Dec 1, 2008

The possibility of a direct mitochondrial action of Na ϩ /H ϩ exchanger-1 (NHE-1) inhibitors decr... more The possibility of a direct mitochondrial action of Na ϩ /H ϩ exchanger-1 (NHE-1) inhibitors decreasing reactive oxygen species (ROS) production was assessed in cat myocardium. Angiotensin II and endothelin-1 induced an NADPH oxidase (NOX)-dependent increase in anion superoxide (O 2 Ϫ) production detected by chemiluminescence. Three different NHE-1 inhibitors [cariporide, BIIB-723, and EMD-87580] with no ROS scavenger activity prevented this increase. The mitochondria appeared to be the source of the NOX-dependent ROS released by the "ROSinduced ROS release mechanism" that was blunted by the mitochondrial ATP-sensitive potassium channel blockers 5-hydroxydecanoate and glibenclamide, inhibition of complex I of the electron transport chain with rotenone, and inhibition of the permeability transition pore (MPTP) by cyclosporin A. Cariporide also prevented O 2 Ϫ production induced by the opening of mKATP with diazoxide. Ca 2ϩ-induced swelling was evaluated in isolated mitochondria as an indicator of MPTP formation. Cariporide decreased mitochondrial swelling to the same extent as cyclosporin A and bongkrekic acid, confirming its direct mitochondrial action. Increased O2 Ϫ production, as expected, stimulated ERK1/2 and p90 ribosomal S6 kinase phosphorylation. This was also prevented by cariporide, giving additional support to the existence of a direct mitochondrial action of NHE-1 inhibitors in preventing ROS release. In conclusion, we report a mitochondrial action of NHE-1 inhibitors that should lead us to revisit or reinterpret previous landmark observations about their beneficial effect in several cardiac diseases, such as ischemia-reperfusion injury and cardiac hypertrophy and failure. Further studies are needed to clarify the precise mechanism and site of action of these drugs in blunting MPTP formation and ROS release.

Tercera Época, Oct 1, 2010

Plants, Jul 12, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids, Sep 1, 2023

Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, 2007

In vitro and in vivo activity of berberine on the blood trypomastigote from Trypanosoma cruzi [Ac... more In vitro and in vivo activity of berberine on the blood trypomastigote from Trypanosoma cruzi [Actividad de la berberina en tripomastigotes sanguíneos de Trypanosoma cruzi]

Introducción La administración neonatal de monosodio L-glutamato (MSG) a ratas genera daño a nive... more Introducción La administración neonatal de monosodio L-glutamato (MSG) a ratas genera daño a nivel de neuronas del núcleo arcuato hipotalámico, lo cual determina en la adultez anormalidades morfológicas, neuroendocrinas y del comportamiento, similares a las observadas en el Síndrome Metabólico (SM) humano. Por otro lado, se reconoce un rol patogénico clave del estrés oxidativo en numerosas enfermedades, incluido el mencionado SM. En consecuencia, el objetivo del presente trabajo fue evaluar los efectos de la administración de N-acetil cisteína (NAC), un reconocido antioxidante, que además promueve el incremento en la síntesis de GSH, sobre las alteraciones ocasionadas por el tratamiento neonatal con MSG.

Journal of Medicine and Life, 2018

In the original publication, the authors’ names and their affiliations have been incorrectly type... more In the original publication, the authors’ names and their affiliations have been incorrectly typeset. The original article is now corrected.

International Journal of Fertility & Sterility, 2021

Background We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) suppleme... more Background We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzo- thiazoline-6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs. CG and -57.3% vs. FOEG...

Introducción y Objetivo Cuando el corazón es sometido a un período breve de isquemia seguido de r... more Introducción y Objetivo Cuando el corazón es sometido a un período breve de isquemia seguido de reperfusión, ocurre una sobrecarga de Ca2+ y un aumento en las especies reactivas del oxígeno (ROS) y del nitrógeno (RNS) que conducen a daños que van desde una disminución transitoria de la contractilidad (atontamiento) hasta la aparición de arritmias o la muerte celular. Nosotros demostramos que en el atontamiento aumentan la fosforilación y las oxidaciones reversibles (S-nitrosilación y S-glutationilación) del receptor de rianodina (RyR2). Mientras que el aumento de la fosforilación del RyR2 incrementó la pérdida de Ca2+ del retículo sarcoplasmático con la consecuente generación de arritmias, los efectos de las oxidaciones reversibles fueron contradictorios. La inhibición de manera selectiva de la S-glutationilación o de la S-nitrosilación del RyR2, se asoció a un mayor número de arritmias y deterioro contráctil. Sin embargo, la abolición simultánea de ambas modificaciones redox con un...

Las garrapatas duras (ixodidae) se encuentran ampliamente distribuidas a nivel mundial, constituy... more Las garrapatas duras (ixodidae) se encuentran ampliamente distribuidas a nivel mundial, constituyendo un serio problema economico y de salud publica. La especie mas relevante para la ganaderia bovina es Boophilus microplus, debido a su amplia distribucion, a los danos que ocasiona y a la transmision de enfermedades. Diferentes metodos han sido propuestos para el control de garrapatas, siendo el quimico el mas difundido. El uso indiscriminado de estos compuestos dio lugar a contaminacion, afectando poblaciones no blanco, ocasionando peligros en la salud publica y promoviendo el desarrollo de resistencia a esas sustancias por parte de las poblaciones de garrapatas. Ante esta situacion se investigan en distintas partes del mundo diferentes opciones para el control de las garrapatas. El objetivo de este trabajo fue investigar la repelencia de larvas de B. microplus ocasionada por extractos de plantas medicinales para identificar posteriormente los compuestos quimicos presentes en ellos.

Phytotherapy in the management of diabetes and hypertension, 2020

Olive leaves are used in Mediterranean folk medicine for the treatment of diabetes, hypertension ... more Olive leaves are used in Mediterranean folk medicine for the treatment of diabetes, hypertension and hypercholesterolemia since Ancient times. In the last decades, different authors have studied its chemical composition and ratified its pharmacological properties both in vitro and in vivo and, more recently, clinical trials focusing on its effects on diabetes and hypertension have been developed. In this chapter, information about recent studies on olive leaf are compiled, including its effects on the specific subject of this chapter, but also on other potential pharmacological effects.Fil: Rios, José Luis. Universidad de Valencia; EspañaFil: Andujar, Isabel. Universidad de Valencia; España. Universidad Europea de Valencia; EspañaFil: González Arbeláez, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Universidad Arturo Jauretche; ArgentinaFil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentin

Tercera Época, Apr 1, 2016

Revista de fitoterapia, 2016

Tercera Época, Oct 1, 2011

Pathophysiology, Dec 1, 2018

The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not c... more The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not completely understood, not even the pathways that determine clinical manifestations associated to systemic protein deposition in organs such as liver, kidney and heart. About twenty natural variants of apoA-I were described as inducing amyloidosis, but the mechanisms driving their aggregation and deposition are still unclear. We previously identified that the mutant Gly26Arg but not Lys107-0 induced the release of cytokines and reactive oxygen species from cultured RAW 264.7 murine macrophages, suggesting that part of the pathogenic pathway could elicit of an inflammatory signal. In this work we gained deep insight into this mechanism and determined that Gly26Arg induced a specific pro-inflammatory cascade involving activation of NF-B and its translocation into the nucleus. These findings suggest that some but not all apoA-I natural variants might promote a pro-oxidant microenvironment which could in turn result in oxidative processing of the variants into a misfolded conformation.

Tercera Época, Nov 1, 2014

Journal of Pharmacy and Pharmacology, Mar 1, 2002

The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extrac... more The anti-in ammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) and ethyl phenylpropiolate in the mouse ear, by serotonin and phospholipase A 2 (PLA 2) in the mouse paw, on chronic in ammation induced by repeated application of TPA in the mouse ear and on the delayed-type hypersensitivity induced by sheep red blood cells suggest that said anti-in ammatory activity is due to the effects of compounds expressed via a corticoid-like mechanism. In addition, the antioxidant activity of the extracts seems to be implicated in this anti-in ammatory activity, as the former inhibits enzymatic and non-enzymatic lipid peroxidation and has free-radical scavenger properties. We conclude that the antiin ammatory activity of Helichrysum italicum can be explained by multiple effects, including in ammatory enzyme inhibition, free-radical scavenging activity and corticoid-like effects.

Planta Medica, Jul 1, 2015

Journal of Applied Physiology, Dec 1, 2008

The possibility of a direct mitochondrial action of Na ϩ /H ϩ exchanger-1 (NHE-1) inhibitors decr... more The possibility of a direct mitochondrial action of Na ϩ /H ϩ exchanger-1 (NHE-1) inhibitors decreasing reactive oxygen species (ROS) production was assessed in cat myocardium. Angiotensin II and endothelin-1 induced an NADPH oxidase (NOX)-dependent increase in anion superoxide (O 2 Ϫ) production detected by chemiluminescence. Three different NHE-1 inhibitors [cariporide, BIIB-723, and EMD-87580] with no ROS scavenger activity prevented this increase. The mitochondria appeared to be the source of the NOX-dependent ROS released by the "ROSinduced ROS release mechanism" that was blunted by the mitochondrial ATP-sensitive potassium channel blockers 5-hydroxydecanoate and glibenclamide, inhibition of complex I of the electron transport chain with rotenone, and inhibition of the permeability transition pore (MPTP) by cyclosporin A. Cariporide also prevented O 2 Ϫ production induced by the opening of mKATP with diazoxide. Ca 2ϩ-induced swelling was evaluated in isolated mitochondria as an indicator of MPTP formation. Cariporide decreased mitochondrial swelling to the same extent as cyclosporin A and bongkrekic acid, confirming its direct mitochondrial action. Increased O2 Ϫ production, as expected, stimulated ERK1/2 and p90 ribosomal S6 kinase phosphorylation. This was also prevented by cariporide, giving additional support to the existence of a direct mitochondrial action of NHE-1 inhibitors in preventing ROS release. In conclusion, we report a mitochondrial action of NHE-1 inhibitors that should lead us to revisit or reinterpret previous landmark observations about their beneficial effect in several cardiac diseases, such as ischemia-reperfusion injury and cardiac hypertrophy and failure. Further studies are needed to clarify the precise mechanism and site of action of these drugs in blunting MPTP formation and ROS release.

Tercera Época, Oct 1, 2010

Plants, Jul 12, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids, Sep 1, 2023

Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, 2007

In vitro and in vivo activity of berberine on the blood trypomastigote from Trypanosoma cruzi [Ac... more In vitro and in vivo activity of berberine on the blood trypomastigote from Trypanosoma cruzi [Actividad de la berberina en tripomastigotes sanguíneos de Trypanosoma cruzi]

Introducción La administración neonatal de monosodio L-glutamato (MSG) a ratas genera daño a nive... more Introducción La administración neonatal de monosodio L-glutamato (MSG) a ratas genera daño a nivel de neuronas del núcleo arcuato hipotalámico, lo cual determina en la adultez anormalidades morfológicas, neuroendocrinas y del comportamiento, similares a las observadas en el Síndrome Metabólico (SM) humano. Por otro lado, se reconoce un rol patogénico clave del estrés oxidativo en numerosas enfermedades, incluido el mencionado SM. En consecuencia, el objetivo del presente trabajo fue evaluar los efectos de la administración de N-acetil cisteína (NAC), un reconocido antioxidante, que además promueve el incremento en la síntesis de GSH, sobre las alteraciones ocasionadas por el tratamiento neonatal con MSG.

Journal of Medicine and Life, 2018

In the original publication, the authors’ names and their affiliations have been incorrectly type... more In the original publication, the authors’ names and their affiliations have been incorrectly typeset. The original article is now corrected.

International Journal of Fertility & Sterility, 2021

Background We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) suppleme... more Background We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzo- thiazoline-6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs. CG and -57.3% vs. FOEG...

Introducción y Objetivo Cuando el corazón es sometido a un período breve de isquemia seguido de r... more Introducción y Objetivo Cuando el corazón es sometido a un período breve de isquemia seguido de reperfusión, ocurre una sobrecarga de Ca2+ y un aumento en las especies reactivas del oxígeno (ROS) y del nitrógeno (RNS) que conducen a daños que van desde una disminución transitoria de la contractilidad (atontamiento) hasta la aparición de arritmias o la muerte celular. Nosotros demostramos que en el atontamiento aumentan la fosforilación y las oxidaciones reversibles (S-nitrosilación y S-glutationilación) del receptor de rianodina (RyR2). Mientras que el aumento de la fosforilación del RyR2 incrementó la pérdida de Ca2+ del retículo sarcoplasmático con la consecuente generación de arritmias, los efectos de las oxidaciones reversibles fueron contradictorios. La inhibición de manera selectiva de la S-glutationilación o de la S-nitrosilación del RyR2, se asoció a un mayor número de arritmias y deterioro contráctil. Sin embargo, la abolición simultánea de ambas modificaciones redox con un...

Las garrapatas duras (ixodidae) se encuentran ampliamente distribuidas a nivel mundial, constituy... more Las garrapatas duras (ixodidae) se encuentran ampliamente distribuidas a nivel mundial, constituyendo un serio problema economico y de salud publica. La especie mas relevante para la ganaderia bovina es Boophilus microplus, debido a su amplia distribucion, a los danos que ocasiona y a la transmision de enfermedades. Diferentes metodos han sido propuestos para el control de garrapatas, siendo el quimico el mas difundido. El uso indiscriminado de estos compuestos dio lugar a contaminacion, afectando poblaciones no blanco, ocasionando peligros en la salud publica y promoviendo el desarrollo de resistencia a esas sustancias por parte de las poblaciones de garrapatas. Ante esta situacion se investigan en distintas partes del mundo diferentes opciones para el control de las garrapatas. El objetivo de este trabajo fue investigar la repelencia de larvas de B. microplus ocasionada por extractos de plantas medicinales para identificar posteriormente los compuestos quimicos presentes en ellos.

Yerba Mate is the trivial name of the species Ilex paraguariensis A. St. Hil. (Aquifoliaceae). It... more Yerba Mate is the trivial name of the species Ilex paraguariensis A. St. Hil. (Aquifoliaceae). It has a high reputation in the countries of origin for its psycho-stimulant properties, where the folia are used as a social drink. It is widely distributed in South America. The folium contains caffeine (0.3-1.7%) as principal xanthine, but theobromine is also present. In addition, it contains tannins, essential oils, triterpenes, saponins, resin, and phenolics, principally flavonoids and caffeoyl derivatives, being chlorogenic, isochlorogenic and neochlorogenic acids the most relevant compounds of this last group.
The caffeine confers psycho-stimulant properties as well as cardiovascular and respiratory stimulant properties, which are the base of its anti-fatigue and stimulatory effects. Other properties as thermogenic, digestive, and hypocholesterolemic are also due to the presence of caffeine.
The second relevant group of compounds is the phenolics, that confer anti-oxidant properties to yerba mate, which are closely related with its cardio-protective, anti-mutagenic and cellular protective effects. Reactive oxygen species (ROS) are implicated in different pathologies, especially in cardiovascular diseases but also in digestive disorders and other systems and organs. In particular, a key factor in the development of cardiovascular diseases such as cardiac damage, atherogenesis, or arterial injuries, is the membrane damage caused by lipid peroxidation. In these cases, the use of anti-oxidants, such as carotenes or vitamin E is widely accepted; however, there are evidences that they could be innocuous or deleterious. For this reason, the administration of an anti-oxidant therapy based on the use of natural products with good bioavailability or with activity on anti-oxidant enzymes may protect against the direct injurious effects described for some oxidants.
In the present chapter, we make a critical review on the properties of yerba mate, focusing on the anti-oxidant effects and the differences with other anti-oxidants used for medical purposes.

Este trabajo presenta resultados de una indagación de las dificultades de los estudiantes univers... more Este trabajo presenta resultados de una indagación de las dificultades de los estudiantes universitarios en la comprensión del lenguaje usado por los profesores en la enseñanza. Se examinaron
algunas dificultades frecuentes en la comprensión del significado de palabras específicas de las materias de estudio. Interesa mostrar cómo en las aulas se generan situaciones de poder, donde
los profesores son quienes institucionalizan, regulan y gobiernan el flujo de la comunicación. Leer, discutir y escribir fueron los recursos de trabajo empleados.