SONIA CALLE | Universidad Nacional Mayor de San Marcos (original) (raw)
Papers by SONIA CALLE
Revista de Investigaciones Veterinarias del Perú, 2012
The aim of the study was to determine the time of infection with Mycoplasma hyopneumoniae in 240 ... more The aim of the study was to determine the time of infection with Mycoplasma hyopneumoniae in 240 piglets of vaccinated and non vaccinated sows from a positive farm with an all-in/all-out (AIAO) system, located in the Chillón valley, Lima. The study was conducted during autumn and winter 2003. One group of sows was vaccinated with 2 ml of a M. hyopneumoniae commercial bacterin at 85 days of gestation while the other group remained unvaccinated. Two groups of 120 piglets each were established according to their dams' status of immunity. Blood samples were collected from piglets at various times during the production period (weaning till slaughter) for measuring antibodies against M. hyopneumoniae using an ELISA indirect assay. The 66.6 (74/112) and 85.6% (101/118) of piglets from the vaccinated and non-vaccinated dams, respectively, were seroreactive throughout the period of the study. The results indicated that the time of infection occurred in both groups at approximately 10 weeks of age, and without significant differences between groups (p>0.05). Time of infection occurred concomitantly with the stress caused by the relocation of piglets from nursery to growth-finishing corrals.
Revista de investigaciones veterinarias del Perú, Oct 27, 2022
Marcos. Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creat... more Marcos. Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creative Commons Atribución 4.0 Internacional (CC BY 4.0) [https:// creativecommons.org/licenses/by/4.0/deed.es] que permite el uso, distribución y reproducción en cualquier medio, siempre que la obra original sea debidamente citada de su fuente original Detección de genes de resistencia antimicrobiana en aislados de Escherichia coli de cerdos de producción con cuadros diarreicos Detection of antimicrobial resistance genes in isolates of Escherichia coli from production pigs with diarrheal diseases
Revista de investigaciones veterinarias del Perú, Mar 4, 2019
El objetivo del estudio fue detectar fenotípicamente los mecanismos de resistencia antimicrobiana... more El objetivo del estudio fue detectar fenotípicamente los mecanismos de resistencia antimicrobiana de 36 aislados de Escherichia coli a betalactámicos, quinolonas y aminoglucósidos mediante la técnica de Kirby-Bauer. Se utilizaron 36 aislados de E. coli procedentes de porcinos de granjas tecnificadas, obtenidos durante el periodo 2010-2015. Se utilizaron 15 antimicrobianos de importancia en medicina humana y veterinaria. Se detectó resistencia principalmente al ácido nalidíxico (89%, 32/36), cloxacilina (83%, 30/36) y amoxicilina-ácido clavulánico (69%, 25/36). Solo un 3% (1/36) presentó AmpC inducible, 42% (15/36) evidenció una posible mutación en gyrA y el 14% (5/36) al menos dos posibles mutaciones en gyrA o gyrA+parC. Además, el 33% (12/36) evidenció altas probabilidades de presencia de genes qnr. Las enzimas del mecanismo de resistencia a aminoglucósidos fueron positivas en un 39% (14/36) de AAC (6'), 28% (10/36) ANT (2") y 11% (4/36) de AAC (3) IV.
Revista de Investigaciones Veterinarias del Perú (RIVEP), 2020
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised... more Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nationwide survey on antimicrobial resistance in animal pathogens in Australia for a oneyear period (January 2013 to January 2014). Clinical Staphylococcus spp. isolates (n = 888) obtained from 22 veterinary diagnostic laboratories were identified by MALDI-TOF mass spectrometry and subjected to antimicrobial susceptibility testing for 16 antimicrobials, representing 12 antimicrobial classes. Potential risk factors associated with methicillin resistance in Staphylococcus pseudintermedius isolates from dogs were analysed based on demographic factors and clinical history, including gender, age, previous antimicrobial treatment, chronic and/or recurrent diseases and site of infections. The most commonly identified CoPS were S. pseudintermedius (70.8%; dogs n = 616, cats n = 13) and S. aureus (13.2%, horses n = 53, dogs n = 47 and cats n = 17). Overall, the frequency of methicillin resistance among S. pseudintermedius (MRSP) and S. aureus (MRSA) was 11.8% and 12.8%, respectively. MRSP isolates were strongly associated with resistance to fluoroquinolones (OR 287; 95%CI 91.2-1144.8) and clindamycin (OR 105.2, 95%CI 48.5-231.9). MRSA isolates from dogs and cats were also more likely to be resistant to fluoroquinolones (OR 5.4, 95%CI 0.6-252.1), whereas MRSA from horses were more likely to be resistant to rifampicin. In multivariate analysis, MRSP-positive status was significantly associated with particular infection sites, including surgical (OR 8.8; 95%CI 3.74-20.7), and skin and soft tissue (OR 3.9; 95%CI 1.97-7.51). S. pseudintermedius isolated from dogs with surgical site infections were three times more likely to be methicillin-resistant if cases had received prior antimicrobial treatment. Whilst the survey results indicate the proportion of CoPS obtained from Australian companion animals that are methicillin-resistant is currently moderate, the
Sustainability
The Principles for Responsible Investment in Agriculture and Food Systems (CFS-RAI) are shown as ... more The Principles for Responsible Investment in Agriculture and Food Systems (CFS-RAI) are shown as suitable instruments to contribute to the Sustainable Development Goals (SDG) in rural areas. Local Action Groups (LAGs) have proven to be effective governance structures for the implementation and management of rural development projects based on participation and collective action. This research integrates the implementation of the LAG from the CFS-RAI Principles for the design of a rural development strategy. The foundations of these new structures are analyzed and the process of creating a LAG linked to the El Mantaro Regional Development Center (RDC) of the Universidad Nacional Mayor de San Marcos (UNMSM), in the Junín region (Peru), is described. The methodological process incorporates the “Working With People” (WWP) approach and the LEADER (Liaison Entre Actions de Développement de l’Économie Rurale) specificities for the analysis of empirical information obtained in surveys and w...
Revista de Investigaciones Veterinarias del Perú
El objetivo del presente estudio fue identificar molecular y serológicamente Salmonella spp prese... more El objetivo del presente estudio fue identificar molecular y serológicamente Salmonella spp presentes en aislados de huevos, canales y vísceras aviares. Se evaluaron 46 aislados de origen aviar identificados como Salmonella spp mediante cultivos y pruebas bioquímicas en el periodo 2012-2017, procedentes de varios distritos de la ciudad de Lima. Estos aislados son conservados en el cepario de Laboratorio de Bacteriología de la Universidad Nacional Mayor de San Marcos. Se reactivaron las cepas, y se reconfirmaron las colonias sospechosas a Salmonella spp mediante el cultivo en medios selectivos. Posteriormente se realizó el PCR a las muestras sospechosas como método de diagnóstico genético de Salmonella. Se detectó el gen de invasividad invA, gen involucrado con la virulencia de Salmonella. Se realizó la serotipificación con antisueros polivalentes y monovalentes para serogrupo y serovar en el Laboratorio de Enteropatógenos del Instituto Nacional de Salud (INS), y finalmente se determ...
Universitat Oberta de Catalunya (UOC), Jun 1, 2021
Universidad Autónoma de Manizales eBooks, 2015
Con el objetivo de determinar la prevalecencia de helicobacter pylori (Hp) en pacientes con gingi... more Con el objetivo de determinar la prevalecencia de helicobacter pylori (Hp) en pacientes con gingivitis y enfermedad periodontal, se colecto y proceso muestras de placa subgingival de 117 pacientes categorizados segun los siguientes antecedentes registrados: 1) ulcera gingivitis, 2)gastritis v gingivitis, 3) gingivitis y 4) periodontitis, aleatoriamente seleccionados en la Clinica a Odontologica de la Universidad de San Marcos. Se recupero Hp en 9,4% de la poblacion en estudio, mas frecuentemente en mujeres (6,8%) que en hombres (2,6%). Helicobacter pylori se recupero en el 7,7 % de pacientes con gingivitis, y en el 1, 7 % de pacientes con gastritis y gingivitis. No se recupero de pacientes con ulcera y gingivitis, ni de pacientes con periodontitis. Hp se recupero mayormente en los estratos Ile 20 a 30 afios, y mas frecuente en mujeres que hombres.
En base a los resultados obtenidos sobre el hallazgo de Helicobacter pylori en los estudios prece... more En base a los resultados obtenidos sobre el hallazgo de Helicobacter pylori en los estudios precedentes, en Placa Dental, y en pacientes con gi. ngivitis y enfermedad periodontal, (Moronn, 1999 y 2000, respectivamente), se considero necesario implementar este proyecto, mediante la determinacion de los niveles de infeccion a traves de la presencia de anticuerpos especificos. En este caso el estudio abarcara una poblacion de estudio mas amplia, constituida por una muestra poblacional de estudiantes de In Facultad de Odontologia de la Universidad de San Marcos, con el objeto de conocer la situacion estudiantil respecto al nivel de anticuerpos contra Helicobacterpylori.
With the objective of prove the prevalence of the Helicobacter pylori (flp) in odontology student... more With the objective of prove the prevalence of the Helicobacter pylori (flp) in odontology students and establish the relation between the positive seroreactors and related symptomatologies to Hp, like: stress, gastritis, ulcer and cancer it was selected a random population sample of 91 students between 15-24 years old, for the detection of IgG antibodies in sera using the Kit Platelia R. To the same population sample it was applied a personal information form lo be filled by the students. Front a total of 91 students that have finished the study, it was founded a general seroprevalence of the Hp in 72,5 % (66/91), with sexual prevalence of 68,3 and 76 %, in women and men, respectively. At the time of relate the personal information, with the positive seroreactors, it was founded that: the 73,1 % (19/26) have stress, 61,5 % (8/13) have gastritis y 100 % (2/2) have ulcer. With regard to the symptomatologies in the student of the sample, it was founded that: the 28,6 % (26/91) have str...
Revista de Investigaciones Veterinarias del Perú, 2012
departamento de Huánuco, en el año 2007. Se colectaron muestras de sangre de 3221 animales para e... more departamento de Huánuco, en el año 2007. Se colectaron muestras de sangre de 3221 animales para el diagnóstico de Brucella sp. mediante la prueba de aglutinación Rosa de Bengala. No se encontraron reactores positivos, y con el programa @Risk de simulaciones estocástica de distribución beta se calculó una prevalencia media de 0.031% con rangos de 0.0008 a 0.1144%. La baja prevalencia permitiría implementar un programa de erradicación de brucelosis bovina en el distrito de Puerto Inca.
Revista de Investigaciones Veterinarias del Perú, 2012
The aim of the study was to determine the time of infection with Mycoplasma hyopneumoniae in 240 ... more The aim of the study was to determine the time of infection with Mycoplasma hyopneumoniae in 240 piglets of vaccinated and non vaccinated sows from a positive farm with an all-in/all-out (AIAO) system, located in the Chillón valley, Lima. The study was conducted during autumn and winter 2003. One group of sows was vaccinated with 2 ml of a M. hyopneumoniae commercial bacterin at 85 days of gestation while the other group remained unvaccinated. Two groups of 120 piglets each were established according to their dams' status of immunity. Blood samples were collected from piglets at various times during the production period (weaning till slaughter) for measuring antibodies against M. hyopneumoniae using an ELISA indirect assay. The 66.6 (74/112) and 85.6% (101/118) of piglets from the vaccinated and non-vaccinated dams, respectively, were seroreactive throughout the period of the study. The results indicated that the time of infection occurred in both groups at approximately 10 weeks of age, and without significant differences between groups (p>0.05). Time of infection occurred concomitantly with the stress caused by the relocation of piglets from nursery to growth-finishing corrals.
Revista de investigaciones veterinarias del Perú, Oct 27, 2022
Marcos. Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creat... more Marcos. Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creative Commons Atribución 4.0 Internacional (CC BY 4.0) [https:// creativecommons.org/licenses/by/4.0/deed.es] que permite el uso, distribución y reproducción en cualquier medio, siempre que la obra original sea debidamente citada de su fuente original Detección de genes de resistencia antimicrobiana en aislados de Escherichia coli de cerdos de producción con cuadros diarreicos Detection of antimicrobial resistance genes in isolates of Escherichia coli from production pigs with diarrheal diseases
Revista de investigaciones veterinarias del Perú, Mar 4, 2019
El objetivo del estudio fue detectar fenotípicamente los mecanismos de resistencia antimicrobiana... more El objetivo del estudio fue detectar fenotípicamente los mecanismos de resistencia antimicrobiana de 36 aislados de Escherichia coli a betalactámicos, quinolonas y aminoglucósidos mediante la técnica de Kirby-Bauer. Se utilizaron 36 aislados de E. coli procedentes de porcinos de granjas tecnificadas, obtenidos durante el periodo 2010-2015. Se utilizaron 15 antimicrobianos de importancia en medicina humana y veterinaria. Se detectó resistencia principalmente al ácido nalidíxico (89%, 32/36), cloxacilina (83%, 30/36) y amoxicilina-ácido clavulánico (69%, 25/36). Solo un 3% (1/36) presentó AmpC inducible, 42% (15/36) evidenció una posible mutación en gyrA y el 14% (5/36) al menos dos posibles mutaciones en gyrA o gyrA+parC. Además, el 33% (12/36) evidenció altas probabilidades de presencia de genes qnr. Las enzimas del mecanismo de resistencia a aminoglucósidos fueron positivas en un 39% (14/36) de AAC (6'), 28% (10/36) ANT (2") y 11% (4/36) de AAC (3) IV.
Revista de Investigaciones Veterinarias del Perú (RIVEP), 2020
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised... more Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nationwide survey on antimicrobial resistance in animal pathogens in Australia for a oneyear period (January 2013 to January 2014). Clinical Staphylococcus spp. isolates (n = 888) obtained from 22 veterinary diagnostic laboratories were identified by MALDI-TOF mass spectrometry and subjected to antimicrobial susceptibility testing for 16 antimicrobials, representing 12 antimicrobial classes. Potential risk factors associated with methicillin resistance in Staphylococcus pseudintermedius isolates from dogs were analysed based on demographic factors and clinical history, including gender, age, previous antimicrobial treatment, chronic and/or recurrent diseases and site of infections. The most commonly identified CoPS were S. pseudintermedius (70.8%; dogs n = 616, cats n = 13) and S. aureus (13.2%, horses n = 53, dogs n = 47 and cats n = 17). Overall, the frequency of methicillin resistance among S. pseudintermedius (MRSP) and S. aureus (MRSA) was 11.8% and 12.8%, respectively. MRSP isolates were strongly associated with resistance to fluoroquinolones (OR 287; 95%CI 91.2-1144.8) and clindamycin (OR 105.2, 95%CI 48.5-231.9). MRSA isolates from dogs and cats were also more likely to be resistant to fluoroquinolones (OR 5.4, 95%CI 0.6-252.1), whereas MRSA from horses were more likely to be resistant to rifampicin. In multivariate analysis, MRSP-positive status was significantly associated with particular infection sites, including surgical (OR 8.8; 95%CI 3.74-20.7), and skin and soft tissue (OR 3.9; 95%CI 1.97-7.51). S. pseudintermedius isolated from dogs with surgical site infections were three times more likely to be methicillin-resistant if cases had received prior antimicrobial treatment. Whilst the survey results indicate the proportion of CoPS obtained from Australian companion animals that are methicillin-resistant is currently moderate, the
Sustainability
The Principles for Responsible Investment in Agriculture and Food Systems (CFS-RAI) are shown as ... more The Principles for Responsible Investment in Agriculture and Food Systems (CFS-RAI) are shown as suitable instruments to contribute to the Sustainable Development Goals (SDG) in rural areas. Local Action Groups (LAGs) have proven to be effective governance structures for the implementation and management of rural development projects based on participation and collective action. This research integrates the implementation of the LAG from the CFS-RAI Principles for the design of a rural development strategy. The foundations of these new structures are analyzed and the process of creating a LAG linked to the El Mantaro Regional Development Center (RDC) of the Universidad Nacional Mayor de San Marcos (UNMSM), in the Junín region (Peru), is described. The methodological process incorporates the “Working With People” (WWP) approach and the LEADER (Liaison Entre Actions de Développement de l’Économie Rurale) specificities for the analysis of empirical information obtained in surveys and w...
Revista de Investigaciones Veterinarias del Perú
El objetivo del presente estudio fue identificar molecular y serológicamente Salmonella spp prese... more El objetivo del presente estudio fue identificar molecular y serológicamente Salmonella spp presentes en aislados de huevos, canales y vísceras aviares. Se evaluaron 46 aislados de origen aviar identificados como Salmonella spp mediante cultivos y pruebas bioquímicas en el periodo 2012-2017, procedentes de varios distritos de la ciudad de Lima. Estos aislados son conservados en el cepario de Laboratorio de Bacteriología de la Universidad Nacional Mayor de San Marcos. Se reactivaron las cepas, y se reconfirmaron las colonias sospechosas a Salmonella spp mediante el cultivo en medios selectivos. Posteriormente se realizó el PCR a las muestras sospechosas como método de diagnóstico genético de Salmonella. Se detectó el gen de invasividad invA, gen involucrado con la virulencia de Salmonella. Se realizó la serotipificación con antisueros polivalentes y monovalentes para serogrupo y serovar en el Laboratorio de Enteropatógenos del Instituto Nacional de Salud (INS), y finalmente se determ...
Universitat Oberta de Catalunya (UOC), Jun 1, 2021
Universidad Autónoma de Manizales eBooks, 2015
Con el objetivo de determinar la prevalecencia de helicobacter pylori (Hp) en pacientes con gingi... more Con el objetivo de determinar la prevalecencia de helicobacter pylori (Hp) en pacientes con gingivitis y enfermedad periodontal, se colecto y proceso muestras de placa subgingival de 117 pacientes categorizados segun los siguientes antecedentes registrados: 1) ulcera gingivitis, 2)gastritis v gingivitis, 3) gingivitis y 4) periodontitis, aleatoriamente seleccionados en la Clinica a Odontologica de la Universidad de San Marcos. Se recupero Hp en 9,4% de la poblacion en estudio, mas frecuentemente en mujeres (6,8%) que en hombres (2,6%). Helicobacter pylori se recupero en el 7,7 % de pacientes con gingivitis, y en el 1, 7 % de pacientes con gastritis y gingivitis. No se recupero de pacientes con ulcera y gingivitis, ni de pacientes con periodontitis. Hp se recupero mayormente en los estratos Ile 20 a 30 afios, y mas frecuente en mujeres que hombres.
En base a los resultados obtenidos sobre el hallazgo de Helicobacter pylori en los estudios prece... more En base a los resultados obtenidos sobre el hallazgo de Helicobacter pylori en los estudios precedentes, en Placa Dental, y en pacientes con gi. ngivitis y enfermedad periodontal, (Moronn, 1999 y 2000, respectivamente), se considero necesario implementar este proyecto, mediante la determinacion de los niveles de infeccion a traves de la presencia de anticuerpos especificos. En este caso el estudio abarcara una poblacion de estudio mas amplia, constituida por una muestra poblacional de estudiantes de In Facultad de Odontologia de la Universidad de San Marcos, con el objeto de conocer la situacion estudiantil respecto al nivel de anticuerpos contra Helicobacterpylori.
With the objective of prove the prevalence of the Helicobacter pylori (flp) in odontology student... more With the objective of prove the prevalence of the Helicobacter pylori (flp) in odontology students and establish the relation between the positive seroreactors and related symptomatologies to Hp, like: stress, gastritis, ulcer and cancer it was selected a random population sample of 91 students between 15-24 years old, for the detection of IgG antibodies in sera using the Kit Platelia R. To the same population sample it was applied a personal information form lo be filled by the students. Front a total of 91 students that have finished the study, it was founded a general seroprevalence of the Hp in 72,5 % (66/91), with sexual prevalence of 68,3 and 76 %, in women and men, respectively. At the time of relate the personal information, with the positive seroreactors, it was founded that: the 73,1 % (19/26) have stress, 61,5 % (8/13) have gastritis y 100 % (2/2) have ulcer. With regard to the symptomatologies in the student of the sample, it was founded that: the 28,6 % (26/91) have str...
Revista de Investigaciones Veterinarias del Perú, 2012
departamento de Huánuco, en el año 2007. Se colectaron muestras de sangre de 3221 animales para e... more departamento de Huánuco, en el año 2007. Se colectaron muestras de sangre de 3221 animales para el diagnóstico de Brucella sp. mediante la prueba de aglutinación Rosa de Bengala. No se encontraron reactores positivos, y con el programa @Risk de simulaciones estocástica de distribución beta se calculó una prevalencia media de 0.031% con rangos de 0.0008 a 0.1144%. La baja prevalencia permitiría implementar un programa de erradicación de brucelosis bovina en el distrito de Puerto Inca.