Antonio Rulli | University of Perugia, Italy (original) (raw)

Papers by Antonio Rulli

Association among gene polymorphisms and clinical-pathological features. (DOC 99Â kb)

Background: The Primary Breast Lymphomas (PBL) represent 0,38-0,70% of all non-Hodgkin lymphomas ... more Background: The Primary Breast Lymphomas (PBL) represent 0,38-0,70% of all non-Hodgkin lymphomas (NHL), 1,72,2% of all extranodal NHL and only 0,04-0,5% of all breast cancer. Most frequent PBLs are the diffuse large B cell lymphomas; in any case-reports MALT lymphomas lack or are a rare occurrence. Their incidence is growing. From 1880 (first breast resection for "lymphadenoid sarcoma" carried out by Gross) to the recent past the gold standard treatment for such diseases was surgery. At present such role has lost some of its importance, and it is matter of debate. Methods: Twenty-three women affected by PBL underwent surgery. Average age was 63 years (range: 39-83). Seven suffered of hypothyroidism secondary to autoimmune thyroiditis. Fourteen patients underwent mastectomy, nine patients received quadrantectomy (average neoplasm diameter: 1,85 cm, range: 1,1-2,6 cm). In 10 cases axillary dissection was carried out. Pathologic examination revealed 16 diffuse large B cell ly...

The Breast, 2017

Aims: Lipofilling has been developed to correct the problem after breast conserving surgery, brea... more Aims: Lipofilling has been developed to correct the problem after breast conserving surgery, breast reconstruction in the patient with breast cancer. The aim of this study is to evaluate amount and proportion of adipose-derived stromal cell (ADSCs) in harvested fat on lipofilling outcome and other factors effecting outcome of the lipofilling procedure. Methods: A prospective cohort study was performed between 2014 and 2016 in breast cancer and phyllodes tumor patients who underwent breast conservative surgery, total mastectomy with immediate or delayed reconstruction and have the deformity or asymmetry after surgery. All patients had undergone lipofilling procedure using Coleman's technique. Data were collected, CD marker, cell viability and outcome of the lipofilling procedure such as complication, fat necrosis, and local recurrence. Results: Seventeen patients had undergone lipofilling procedure. The mean follow-up time was 11.02 months. One patient had seroma complication at donor site, one patient had breast abscess after the operation. Mammogram and ultrasound were performed in 13 patients. Two patients developed BIRADS 4A (Breast Imaging Reporting And Data system) in mammography and needed tissue diagnosis, one patient had fat necrosis from fine needle aspiration (FNA) and another had fat necrosis from core needle biopsy. Six fat samples were analyzed the cluster of differentiation (CD) marker. The fat graft demonstrated CD 45−, CD34+ in all samples. One sample demonstrated CD 45-CD34+, CD105-, CD90+ confirm that there are ADSCs. No local recurrence was found in the period of follow up time. Conclusion: These data confirm the fat graft has adiposederived cell populations and ADSCs. The lipofilling procedure can use safety in patients who have defect from oncologic breast surgery.

th he d lo se ts d ileitis due to colon cancer with a rapid clinical course, in which shock statu... more th he d lo se ts d ileitis due to colon cancer with a rapid clinical course, in which shock status occurred within ten hour of initial C-reactive protein 2.70 mg/dl, hemoglobin 15.3 mg/dl, and blood urea nitrogen 41 mg/dl, indicative of severe dehydra-

European Journal of Cancer Supplements, 2004

CMF/FEC treatment-related toxicity graded according to the NCI- CTC v.2.0. (DOC 55Â kb)

Annali italiani di chirurgia

Breast surgery is classified among the procedures performed in clean surgery and is associated wi... more Breast surgery is classified among the procedures performed in clean surgery and is associated with a low incidence of wound infection (3-15%). The objective of this study was to evaluate the advantages antibiotic prophylaxis in patients undergoing breast surgery. A multicenter randomized controlled study was performed between January 2008 and November 2008. One thousand four hundred patients were enrolled in prospective randomized study; surgical wound infection was found in 41 patients (2.93%). In our RCT we have shown that in breast surgery antibiotic prophylaxis does not present significant advantages in patients with potential risk of infection (17 patients, 2.42%, subjected to antibiotic prophylaxis vs 24 patients, 3.43%, without antibiotic prophylaxis) (P = 0.27). In patients with drainage there is a significant minor incidence of wound infections in patients receiving antibiotic prophylaxis (5 patients, 0.92%, subjected to antibiotic prophylaxis vs 14 patients, 3.09%, withou...

Biomedical Journal of Scientific & Technical Research, 2021

Background: Breast cancer is distinguished in three different subtypes, based on clinical and mol... more Background: Breast cancer is distinguished in three different subtypes, based on clinical and molecular parameters: Luminal - HER2 - Basal type. Luminal carcinomas (which represent about 65% of the total) are distinguished by a particular heterogeneity of biological behaviour with disease recovery in about 40-50% and death in about two-thirds of these patients at 5 years from diagnosis, despite initial anatomopathological pictures of apparent low aggressiveness. Precisely, more biomolecular parameters are desirable for this diagnostic category which starting from an estimated disease recovery can guide therapeutic choices in a more articulated way that is modelled on the actual needs.Method: The aim of this work is to build a panel of genes that can be used to personalize therapy and consequently reduce mortality. Our kit is patented and includes 33 genes that best characterize neoplastic heterogeneity and sensitivity to drugs. The study involved the total transcriptome (RNA) sequencing of 40 patient samples carried out in the two-year period 1994/1995 with the aim of identifying a group of genes expressed differentially among patients with good prognosis and those with poor prognosis. Results: Total RNA extraction from formalin-fixed, paraffin-embedded samples and then Library preparation for RNA-Seq was achieved. The study highlighted some genes: CXCL13 life gene, IFITM10 death gene always present regardless of molecular subtype, and DSCAM-AS1 gene, specific for Luminal A subtype, that if present, let the patient avoid standard PBI and neoadjuvant therapy. Conclusion: The goal is to implement a different surgical and adjuvant personalized therapy for every single patient.

Minerva chirurgica, 2019

BACKGROUND Radical lymph nodes dissection (RLND) is the standard procedure for regional control o... more BACKGROUND Radical lymph nodes dissection (RLND) is the standard procedure for regional control of disease in patients with metastatic melanoma (stage III). Because of its aggressiveness, lymphorrhoea is a constant problem in post-operative. METHODS In this prospective non randomized study with a retrospective analysis of data, patients undergoing surgical treatment of axillary or iliac-inguinal radical lymph-node dissection (RLND) received standard treatment plus a collagen-fibrin sealant patch (TachoSil) (n=50) or standard treatment alone (n=50). The first endpoint was the comparison between the treated population and the control group, in terms of persistence of drainage and average daily volume of lymphorrhoea. The second aim was to relate patients' BMI, volume of lymphorrhoea and time of drainage in the two groups. RESULTS In the comparison between the two groups receiving or not the treatment with collagen-fibrin patch, we found an average difference both in the volume of ...

The American Surgeon, 2014

Background Liquid biopsy (LB) is a technique that utilizes circulating biomarkers from cancer pat... more Background Liquid biopsy (LB) is a technique that utilizes circulating biomarkers from cancer patients to provide information regarding the genetic landscape of the cancer. LB is emerging as an alternative and complementary diagnostic and prognostic tool to surgical biopsy and is expected to provide the tool for the implementation of precision oncology in clinical settings. In fact, it may contribute to enhance understanding of tumor heterogeneity and permitting the dynamic monitoring of treatment responses and genomic variations. Thus, LB is a promising method for the management of cancer, including breast cancer (BC), whose incidence in Italy is progressively increasing. Previous studies focused mainly on patients with advanced-stage BC. In the present study we evaluated the number of circulating tumor cells (CTCs), the quantity of cell free tumor DNA (cftDNA) and the analysis of the mutational profile of DNA from CTCs (ctcDNA) and cftDNA in early stage BC patients. Methods Matche...

BMC cancer, Jan 26, 2017

We investigated whether GSTT1 ("null" allele), GSTM1 ("null"allele), GSTP1 (A... more We investigated whether GSTT1 ("null" allele), GSTM1 ("null"allele), GSTP1 (A313G), RFC1 (G80A), MTHFR (C677T), TS (2R/3R) polymorphisms were associated with toxicity and survival in patients with early breast cancer (EBC) treated with adjuvant chemotherapy (CT). This prospective trial included patients with stage I-III BC subjected to CT with CMF or FEC regimens. PCR-RFLP was performed for MTHFR, RFC1 and GSTP1, while PCR for TS, GSTT1 and GSTM1 genes. Among the 244 patients consecutively enrolled, 48.7% were treated with FEC and 51.3% with CMF. Patients with TS2R/3R genotype showed less frequently severe neutropenia (G3/G4) than those with TS2R/2R and 3R/3R genotype (p = 0.038). Patients with MTHFRCT genotype had a higher probability of developing severe neutropenia than those with MTHFR CC genotype (p = 0.043). Patients with RFC1GG or GSTT1-null genotype or their combination (GSTT1-null/RFC1GG) were significantly associated with a shorter disease free survival...

Journal of experimental & clinical cancer research : CR, May 16, 2017

In vivo (Athens, Greece)

To state the limits of axillary lymphadenectomy in patients with metastatic melanoma. We performe... more To state the limits of axillary lymphadenectomy in patients with metastatic melanoma. We performed a prospective study on patients submitted to axillary dissection for stage III melanoma. At surgery, the third-level nodes were separately dissected to be examined by the pathologist. We analyzed 86 patients. In 93%, the third-level nodes were disease-free; none of the patients with previous positive sentinel nodes (SN) showed nodal metastases at level III. Patients (7%) found to have positive level III nodes had undergone therapeutic lymphadenectomy for bulky nodal disease. Our data show that axillary non-sentinel nodes of level III are usually disease-free in cases of previously positive SN, while they can be involved in the presence of bulky disease. A prerequisite allowing sparing of level III nodes after a positive SN biopsy is the meticulous research of all level I and II lymph nodes.

Breast Cancer Res Treat, 2006