Grazia P. | University of Perugia, Italy (original) (raw)

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Papers by Grazia P.

Research paper thumbnail of Comparison of aortoiliac repair with iliac branch endoprosthesis versus hypogastric occlusion in aortoiliac aneurysms

Italian Journal of Vascular and Endovascular Surgery, 2019

Research paper thumbnail of Antiproliferative and Proapoptotic Effects of the TrK-inhibitor GW441756 in Human Myosarcomas and Prostatic Carcinoma

Current Signal Transduction Therapy, 2013

Research paper thumbnail of The tricyclic antidepressant amitriptyline is cytotoxic to HTB114 human leiomyosarcoma and induces p75NTR-dependent apoptosis

Anti-Cancer Drugs, 2013

Nerve growth factor (NGF) receptors, TrKA and p75(NTR), are being investigated in cancer therapy.... more Nerve growth factor (NGF) receptors, TrKA and p75(NTR), are being investigated in cancer therapy. Our previous data show that, in HTB114 uterine leiomyosarcoma cells, p75(NTR)-dependent apoptosis is inducible by cytotoxic drugs and can suppress nerve growth factor-dependent growth. Although amitriptyline can kill cancer cells and bind TrKA/B, its effects on p75-dependent apoptosis are unknown. The aim of this paper was to evaluate the antineoplastic potential of amitriptyline, and the role of p75(NTR)-dependent apoptosis in the chemoresistant uterine HTB114 leiomyosarcoma. Using proliferation assays and fluorescence-activated cell sorting analysis, we found that amitriptyline caused a marked reduction in HTB114 cell viability, associated with the parallel upregulation of p75(NTR) expression. This converted the TrKA⁺-proliferating cells into TrKA⁺/p75(NTR⁺), leading to downregulation of TrKA-prosurvival signaling (AKT) and activation of p75(NTR)-dependent apoptosis (through caspase-3). Overall, we provide novel evidence that HTB114 uterine leiomyosarcoma cells are highly sensitive to amitriptyline, supporting the role of p75(NTR)-dependent apoptosis as a novel cytotoxic mechanism of this drug and of p75(NTR) as an inducible stress receptor and a novel target in clinical oncology.

Research paper thumbnail of Novel isoforms of CaBP (Annexin V) from mammalian brain: structural and phosphorylation differences that suggest distinct biological roles

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992

Two calcium-dependent phospholipid-and membrane-binding proteins have been purified from bovine b... more Two calcium-dependent phospholipid-and membrane-binding proteins have been purified from bovine brain. These are termed CaBP33 and CaBP37. Complete sequence analysis has revealed that these two proteins are isoforms of annexin V. Despite an apparent difference of 4 kDa between the two proteins on SDS-PAGE, only two amino-acid substitutions were found. These are, in CaBP33, Ser-36 and Lys-125 and in CaBP37, Thr-36 and Glu-125. This corresponds to a mass difference of 15 Da. This was confirmed by electrospray mass spectrometric analysis. Both isoforms can be phosphorylated substoichiometrically in vitro by protein kinase C at residue Thr-22.

Research paper thumbnail of Second asymptomatic carotid surgery trial (ACST-2): a randomised comparison of carotid artery stenting versus carotid endarterectomy

Research paper thumbnail of Studio del meccanismo di splicing alternativo del gene murino di Annessina VI e della espressione differenziale dei due trascritti in diversi tessuti murini

Research paper thumbnail of Immunocytochemical localization of pgp 9.5 in the central and peripheral nervous system

European Journal of Neuroscience

Research paper thumbnail of CABP33 (Annexin V, Endonexin II) in porcine heart

The Journal of Cell Biology

Research paper thumbnail of Interaction between two brain annexins and brain cytoskeletons

Research paper thumbnail of Caratterizzazione di due nuove annessine (CBP33 e CABP37) purificate da cervello

Research paper thumbnail of Properties of two novel annexins (CABP33 and CABP37) purified from bovine brain

Research paper thumbnail of Characterization of two novel calcium-dependent phospholipid- and membrane-binding proteins purified from bovine brain

Research paper thumbnail of Analisi di una nuova annessina, CABP33, in muscolo cardiaco di mammifero

Research paper thumbnail of Interaction between S100 protein and annexing II (p36, the heavy chain of calpactin I)

The Journal of Cell Biology

Research paper thumbnail of Porcine heart annexins (calcium-dependent phospholipid- and membrane-binding proteins)

Research paper thumbnail of Immunohistochemistry of annexin V (CABP33) in rat tissues

Research paper thumbnail of Bovine CABP33 and CABP37 are isoforms of Annexin V, a calcium-dependent phospholipid- and membrane-binding protein

Research paper thumbnail of A substitution of Lys125 to Glu 125 causes a molecular weight shift on SDS-PAGE of 4 kDa

Research paper thumbnail of Binding of S100 protein to annexin II and p11, the heavy and light chains of the cytoskeletal protein complex calpactin I)

Research paper thumbnail of Annessina (CABP33) cardiaca: purificazione, caratterizzazioen e localizzazione subcellulare

Research paper thumbnail of Comparison of aortoiliac repair with iliac branch endoprosthesis versus hypogastric occlusion in aortoiliac aneurysms

Italian Journal of Vascular and Endovascular Surgery, 2019

Research paper thumbnail of Antiproliferative and Proapoptotic Effects of the TrK-inhibitor GW441756 in Human Myosarcomas and Prostatic Carcinoma

Current Signal Transduction Therapy, 2013

Research paper thumbnail of The tricyclic antidepressant amitriptyline is cytotoxic to HTB114 human leiomyosarcoma and induces p75NTR-dependent apoptosis

Anti-Cancer Drugs, 2013

Nerve growth factor (NGF) receptors, TrKA and p75(NTR), are being investigated in cancer therapy.... more Nerve growth factor (NGF) receptors, TrKA and p75(NTR), are being investigated in cancer therapy. Our previous data show that, in HTB114 uterine leiomyosarcoma cells, p75(NTR)-dependent apoptosis is inducible by cytotoxic drugs and can suppress nerve growth factor-dependent growth. Although amitriptyline can kill cancer cells and bind TrKA/B, its effects on p75-dependent apoptosis are unknown. The aim of this paper was to evaluate the antineoplastic potential of amitriptyline, and the role of p75(NTR)-dependent apoptosis in the chemoresistant uterine HTB114 leiomyosarcoma. Using proliferation assays and fluorescence-activated cell sorting analysis, we found that amitriptyline caused a marked reduction in HTB114 cell viability, associated with the parallel upregulation of p75(NTR) expression. This converted the TrKA⁺-proliferating cells into TrKA⁺/p75(NTR⁺), leading to downregulation of TrKA-prosurvival signaling (AKT) and activation of p75(NTR)-dependent apoptosis (through caspase-3). Overall, we provide novel evidence that HTB114 uterine leiomyosarcoma cells are highly sensitive to amitriptyline, supporting the role of p75(NTR)-dependent apoptosis as a novel cytotoxic mechanism of this drug and of p75(NTR) as an inducible stress receptor and a novel target in clinical oncology.

Research paper thumbnail of Novel isoforms of CaBP (Annexin V) from mammalian brain: structural and phosphorylation differences that suggest distinct biological roles

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992

Two calcium-dependent phospholipid-and membrane-binding proteins have been purified from bovine b... more Two calcium-dependent phospholipid-and membrane-binding proteins have been purified from bovine brain. These are termed CaBP33 and CaBP37. Complete sequence analysis has revealed that these two proteins are isoforms of annexin V. Despite an apparent difference of 4 kDa between the two proteins on SDS-PAGE, only two amino-acid substitutions were found. These are, in CaBP33, Ser-36 and Lys-125 and in CaBP37, Thr-36 and Glu-125. This corresponds to a mass difference of 15 Da. This was confirmed by electrospray mass spectrometric analysis. Both isoforms can be phosphorylated substoichiometrically in vitro by protein kinase C at residue Thr-22.

Research paper thumbnail of Second asymptomatic carotid surgery trial (ACST-2): a randomised comparison of carotid artery stenting versus carotid endarterectomy

Research paper thumbnail of Studio del meccanismo di splicing alternativo del gene murino di Annessina VI e della espressione differenziale dei due trascritti in diversi tessuti murini

Research paper thumbnail of Immunocytochemical localization of pgp 9.5 in the central and peripheral nervous system

European Journal of Neuroscience

Research paper thumbnail of CABP33 (Annexin V, Endonexin II) in porcine heart

The Journal of Cell Biology

Research paper thumbnail of Interaction between two brain annexins and brain cytoskeletons

Research paper thumbnail of Caratterizzazione di due nuove annessine (CBP33 e CABP37) purificate da cervello

Research paper thumbnail of Properties of two novel annexins (CABP33 and CABP37) purified from bovine brain

Research paper thumbnail of Characterization of two novel calcium-dependent phospholipid- and membrane-binding proteins purified from bovine brain

Research paper thumbnail of Analisi di una nuova annessina, CABP33, in muscolo cardiaco di mammifero

Research paper thumbnail of Interaction between S100 protein and annexing II (p36, the heavy chain of calpactin I)

The Journal of Cell Biology

Research paper thumbnail of Porcine heart annexins (calcium-dependent phospholipid- and membrane-binding proteins)

Research paper thumbnail of Immunohistochemistry of annexin V (CABP33) in rat tissues

Research paper thumbnail of Bovine CABP33 and CABP37 are isoforms of Annexin V, a calcium-dependent phospholipid- and membrane-binding protein

Research paper thumbnail of A substitution of Lys125 to Glu 125 causes a molecular weight shift on SDS-PAGE of 4 kDa

Research paper thumbnail of Binding of S100 protein to annexin II and p11, the heavy and light chains of the cytoskeletal protein complex calpactin I)

Research paper thumbnail of Annessina (CABP33) cardiaca: purificazione, caratterizzazioen e localizzazione subcellulare

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