Ilinca Gussi | UNIVERSITY OF MEDICINE AND PHARMACY CAROL DAVILA BUCHAREST (original) (raw)

Papers by Ilinca Gussi

Endocrine, 2005

... 8. Colao A, Di Sarno A, Cappabianca P, Di Somma C, Pivonello R, Lombardi G. Withdrawal of lon... more ... 8. Colao A, Di Sarno A, Cappabianca P, Di Somma C, Pivonello R, Lombardi G. Withdrawal of longterm cabergoline therapy for tumoral and nontumoral hyperprolactinemia. ... 33. Ciccarelli E, Grottoli S, Razzore P, Gaia D, Bertagna A, Cirillo S et al. ...

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2015

The electrical activity of the uterus, i.e. the electrohysterogram (EHG), is one of the most prom... more The electrical activity of the uterus, i.e. the electrohysterogram (EHG), is one of the most prominent tool for preterm labour. There is no standard acquisition set up and often the EHG is corrupted with different types of noise: maternal and fetal electrocardiogram (mECG, fECG), electrical activity of the skeletal muscles, movement artifacts, power line interference (PLI) etc. Moreover, some of these noises overlap in frequency domain with the EHG. Thus, simple linear filtering approaches are not adequate. In this paper the empirical mode decomposition (EMD), a simple and data driven method, is proposed for EHG denoising. The method is evaluated on simulated data having different signal to noise ratios (SNRs) obtaining promising results.

Acta Endocrinologica-bucharest, 2005

... S. Radian1, M. Bensaada4, Corinne Lautier4, JP Molés4, F. Grigorescu*,4 Ilinca Gussi2, C. Bad... more ... S. Radian1, M. Bensaada4, Corinne Lautier4, JP Molés4, F. Grigorescu*,4 Ilinca Gussi2, C. Badiu1, S. Nastasia2, D. Hudita2, Laura Leonte3, B ... 13. Pavel S, Dorcescu M, Petrescu-Holban R, Ghinea E. Biosynthesis of a vasotocin-like peptide in cell cultures from pineal glands of ...

OBJECTIVE The diagnostic impact of chromogranin A (CgA) measurement has been studied in various n... more OBJECTIVE The diagnostic impact of chromogranin A (CgA) measurement has been studied in various neuroendocrine tumours (NET) such as pheochromocytomas, gastrinomas and neuroblastomas. Clinically nonfunctioning pituitary adenomas (NFPA) are generally diagnosed on tumoural symptoms or hypopituitarism and, except for gonadotrophins and their free subunits which may be increased in the case of gonadotrophinomas, markers of endocrine secretory activity are lacking not only for diagnostic purpose but also in the postoperative follow-up of these patients. As the presence of CgA has been demonstrated by immunohistochemistry in pituitary adenomas, we performed this study to further assess the sensitivity of CgA measurement in sporadic pituitary adenomas using a new, specific, sandwich immunoassay. SUBJECTS We first completed a basal normative data set obtained using this assay by studying four healthy men (49 ± ± ± ± 13 years old), five healthy premenopausal women (35·8 ± ± ± ± 7·5 years old) and five healthy postmenopausal women (49·1 ± ± ± ± 4·6 years old) basally and after TRH administration. Twenty-seven patients [12 men (64·2 ± ± ± ± 11·8 years), even premenopausal women (38·4 ± ± ± ± 5·7 years) and eight postmenopausal women (67·7 ± ± ± ± 10·3 years)] with NFPA, 15 acromegalic patients [nine men (45 ± ± ± ± 13·3 years), six women (52 ± ± ± ± 14·9 years)] and 19 patients with a prolactin-secreting adenoma [four men (41·2 ± ± ± ± 18 years) and 15 women (31·2 ± ± ± ± 7·5 years), with a macroadenoma ( n = 11) or a microadenoma ( n = 8)] had basal and TRH-stimulated measurement of CgA. A gonadotrophin-releasing hormone (GnRH)stimulation test was also performed in two, four and four patients, respectively. All patients had sporadic pituitary adenomas.

Stem cell research & therapy, 2013

Human Wharton&amp;amp;amp;#39;s jelly (WJ) has become a preferred source of mesenchymal s... more Human Wharton&amp;amp;amp;#39;s jelly (WJ) has become a preferred source of mesenchymal stem cells (MSCs) whose clinical applications are limited by the use of adequate xeno-free (XF), in vitro manipulation conditions. Therefore, the objective of our study was to characterize WJ-derived MSCs (WJ-MSCs), isolated by different methods and cultured in a commercially available, MSC XF medium, not least of all by investigating their endothelial differentiation capacity. WJ explants and enzymatically dissociated WJ cells were cultured in a defined, XF medium for MSCs. Adherent cells at passages 2 and 5 were characterized as MSCs by flow cytometry, MTT, real-time quantitative reverse transcription PCR, and functional multipotent differentiation assays. The endothelial differentiation capacity of MSCs isolated and expanded until passage 2 in the MSC XF medium, and then subcultured for five passages in a commercially available endothelial growth medium (group A), was assessed over serial passages, as compared to adherent WJ-derived cells isolated and expanded for five consecutive passages in the endothelial medium (group B). The MSC phenotype of WJ explant- and pellet-derived cells, isolated and expanded in the MSC XF medium, was proven based on the expression of CD44/CD73/CD90/CD105 surface markers and osteo-/adipo-/chondrogenic multipotent differentiation potential, which differed according to the isolation method and/or passage number. Upon exposure to endothelial differentiation cues, cells belonging to group A did not exhibit endothelial cell characteristics over serial passages; by contrast, WJ pellet-derived cells belonging to group B expressed endothelial characteristics at gene, protein and functional levels, potentially due to culture conditions favoring the isolation of other stem/progenitor cell types than MSCs, able to give rise to an endothelial progeny. The use of defined, MSC XF media for isolation and expansion of human WJ-MSCs is a prerequisite for the establishment of their real endothelial differentiation capacity, as candidates for clinical therapy applications. Thus, the standardization of WJ-MSCs isolation and culture expansion techniques in defined, MSC XF media, for their accurate characterization, would be a priority in the stem cell research field.

Acta Endocrinologica (Bucharest), 2013

Acta Endocrinologica (Bucharest), 2011

IFMBE Proceedings, 2009

Monitoring the fetal heart rate (fHR) and the morphology of fetal electrocardiogram (fECG) during... more Monitoring the fetal heart rate (fHR) and the morphology of fetal electrocardiogram (fECG) during pregnancy is important to suport medical decision while evaluating the health state of the fetus. But when assessing the fECG through the abdominal signals (ADS), its very low amplitude causes a problem, since the fECG representation in the ADS is buried in a mixture of other

2014 International Conference and Exposition on Electrical and Power Engineering (EPE), 2014

Ultrasound in Obstetrics and Gynecology, 2005

Objective: Conventional Pulsatility Index (PI) analysis of the flow velocity waveform (FVW) of ut... more Objective: Conventional Pulsatility Index (PI) analysis of the flow velocity waveform (FVW) of uterine artery Doppler involves velocities throughout the entire cardiac cycle. The range of velocities observed in systole are sensitive to local vessel characteristics of elasticity and recoil. Forward-flow velocities in diastole reflect the degree of 'run-off' during the relaxation phase of the cardiac cycle and hence, are a reflection of placental resistance downstream. We describe a new method of analysis of the forward-flow in diastole, which we have termed the diastolic flow index (DFI) and investigate its performance in the prediction of pre-eclampsia (PET). Methods: Pulsed-wave Doppler was prospectively used to obtain FVWs of uterine artery Doppler between 11-14 weeks and again between 21-23 weeks' gestation in 1500 pregnancies. The velocitytime integral (VTI) of the waveform in diastole was measured. The index was controlled for variations in heart rate (HR) since the length of diastole is rate-dependent. The placental bed is viewed as an end-organ and flow to it must therefore be corrected for body surface area (BSA).

Ultrasound in Obstetrics and Gynecology, 2006

Methods Thirty-nine cases with severe TTTS were treated at 16-26 weeks of gestation. Maternal hem... more Methods Thirty-nine cases with severe TTTS were treated at 16-26 weeks of gestation. Maternal hemodynamic evaluation was performed, including heart rate (HR), arterial pressure and echocardiography with calculation of shortening fraction (SF), left atrial dimensions, stroke volume (SV), cardiac output (CO) and total vascular resistance (TVR), before and 6 h and 24 h after placental surgery. Hemoglobin (Hb), hematocrit (Ht) and protein levels were also measured. Cases were retrospectively divided into groups according to treatment: Group A had laser therapy followed by amnioreduction > 1000 mL (n = 25); Group B had laser therapy followed by amnioreduction < 1000 mL (n = 14).

Ultrasound in Obstetrics and Gynecology, 2004

International Journal of Gynecology & Obstetrics, 2009

American Journal of Obstetrics and Gynecology, 2004

OBJECTIVE: To observe changes in the renin-angiotensin-aldosterone system following the treatment... more OBJECTIVE: To observe changes in the renin-angiotensin-aldosterone system following the treatment of twin-to-twin transfusion syndrome (TTTS).

BJOG: An International Journal of Obstetrics & Gynaecology, 2006

Objective To monitor changes in the maternal renin-angiotensinaldosterone system following laser ... more Objective To monitor changes in the maternal renin-angiotensinaldosterone system following laser therapy and amnioreduction in severe twin-to-twin transfusion syndrome (TTTS).

Ultrasound in Obstetrics & Gynecology, 2014

Advances in Telemedicine: Applications in Various Medical Disciplines and Geographical Regions, 2011

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2013

The analysis of the fetal heart rate (fHR) is important in detecting the fetal distress related w... more The analysis of the fetal heart rate (fHR) is important in detecting the fetal distress related with hypoxic episodes, noticed sometimes during the uterine activity, which can severely affect the fetus. Occasional synchrony between the fHR and the maternal heart rate (mHR) was reported and the mHR shows some variations during pregnancy and labor, especially when the contractions are very strong. The current study proposes a new strategy to investigate the relations between the fHR, the mHR and the uterine activity, by applying the time-variant Partial Directed Coherence (tvPDC).

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2013

The fetal electrocardiogram (fECG) obtained from the abdominal signals, to monitor the wellbeing ... more The fetal electrocardiogram (fECG) obtained from the abdominal signals, to monitor the wellbeing of the fetus, is a weak signal, recorded by placing electrodes on the maternal abdomen surface. When recording the abdominal fECG, the main problem is to separate the fECG from the background noise, including the maternal electrocardiogram (mECG) and/or the power line interference (PLI), this leading to an improved fECG signal to noise ratio (SNR). This paper proposes and evaluates three types of recording configurations, having different reference location, and analyzes the performance of each recording setup, based on the corresponding SNRs, quantitatively evaluated. The fECG extraction is carried out in order to evaluate the performance of each proposed configuration.

Computational and mathematical methods in medicine, 2014

Interference of power line (PLI) (fundamental frequency and its harmonics) is usually present in ... more Interference of power line (PLI) (fundamental frequency and its harmonics) is usually present in biopotential measurements. Despite all countermeasures, the PLI still corrupts physiological signals, for example, electromyograms (EMG), electroencephalograms (EEG), and electrocardiograms (ECG). When analyzing the fetal ECG (fECG) recorded on the maternal abdomen, the PLI represents a particular strong noise component, being sometimes 10 times greater than the fECG signal, and thus impairing the extraction of any useful information regarding the fetal health state. Many signal processing methods for cancelling the PLI from biopotentials are available in the literature. In this review study, six different principles are analyzed and discussed, and their performance is evaluated on simulated data (three different scenarios), based on five quantitative performance indices.