Iman Gatea | University of Baghdad (original) (raw)

Papers by Iman Gatea

Research paper thumbnail of Biological Treatment of Organ Chlorinated Pesticide Using Local Bacterial Isolates

International Journal for Sciences and Technology, 2013

The aim of this research was to isolate microbial isolates with ability of growing in medium with... more The aim of this research was to isolate microbial isolates with ability of growing in medium with presence of added pesticide and may therefore be used for bioremiation of pesticides contaminated sites (Soil and water). A bacterial groups which capable of degradation of chlorinated organic pesticides was isolated from many agricultural soil and contaminated water. After purification the strains were assessed in order to discover their ability to degrade (COP) (α propachlor) with concentration of 25mg/L as carbon source in mineral medium and in rich medium. However, the best three pure strains were able to grow in M.M includes (COP) without enrichment. This group which was composed of three isolates characterized based on their morphological and biochemical characteristic. The isolates were presumptively identified as rhodococcus spp. and Streptomyces albus. Using growth curve as a parameter of (COP) compounds, optimum conditions (Pesticide concentration, temperature, and time) of selected degrading bacterial strains were studied. Results indicated that the optimal temperature was 37°C for all isolates, with best growth at 100mg/L of propachlor by str. albus, in addition of its efficiency to degrade the compound during 48 hr at incubation.

Research paper thumbnail of The inhibitory effect of silver nanoparticles on someespecially bacteria microorganisms, Pesudomonas aeruginosa, Esherichia coli and Staphylococcus aureus

2ND INTERNATIONAL CONFERENCE ON MATERIALS ENGINEERING & SCIENCE (IConMEAS 2019)

Research paper thumbnail of Bioplastic (Poly -3-HYDROXYBUTYRATE) Production by Local Pseudomonas Aeruginosa Isolates Utilizing Waste Cooking Oil

World Journal of Pharmaceutical Research

Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes t... more Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes to the deterioration of the environment and creating severs problems. Synthetic plastic poses threat to the environment, because it is non-biodegradable are now accumulate in the environment at great millions of tons per year.

Research paper thumbnail of Activity Assay of Bacterial Mixed Culture in Biological Treatment of Organochloropesticides

World Journal of Pharmaceutical Research

Research paper thumbnail of Biological Treatment of Organ Chlorinated Pesticide Using Local Bacterial Isolates

International Journal for Sciences and Technology, 2013

The aim of this research was to isolate microbial isolates with ability of growing in medium with... more The aim of this research was to isolate microbial isolates with ability of growing in medium with presence of added pesticide and may therefore be used for bioremiation of pesticides contaminated sites (Soil and water). A bacterial groups which capable of degradation of chlorinated organic pesticides was isolated from many agricultural soil and contaminated water. After purification the strains were assessed in order to discover their ability to degrade (COP) (α propachlor) with concentration of 25mg/L as carbon source in mineral medium and in rich medium. However, the best three pure strains were able to grow in M.M includes (COP) without enrichment. This group which was composed of three isolates characterized based on their morphological and biochemical characteristic. The isolates were presumptively identified as rhodococcus spp. and Streptomyces albus. Using growth curve as a parameter of (COP) compounds, optimum conditions (Pesticide concentration, temperature, and time) of selected degrading bacterial strains were studied. Results indicated that the optimal temperature was 37°C for all isolates, with best growth at 100mg/L of propachlor by str. albus, in addition of its efficiency to degrade the compound during 48 hr at incubation.

Research paper thumbnail of Biosynthesis of Medium Chain Length Poly-β-hydroxybutyrate by Pseudomonas aeruginosa Dw7

Polyhydroxya lkanoates (PHAs), are a promising family of bio-based polymers, which considered to ... more Polyhydroxya lkanoates (PHAs), are a promising family of bio-based polymers, which considered to be alternatives to traditional petroleum-based plastics. Poly-β-hydroxybutyrate (PHB) is the most known degradable biopolymers, produced by bacterial genera. It is generally accepted that PHB can be used instead of plastic to solve one of the greatest problems facing the environment. Pseudomonas aeruginosa Dw7 was grown on mineral salt medium supplemented with waste cooking of corn oil for the synthesis of a medium chain-PHB. This study is divided into three steps, at first fermentation of P.seudomonas aeruginosa Dw7, which was carried out aerobically at optimum temperature of 30˚C and 500 rpm of agitation speed. Lab scale bioreactor (5L) operated as a batch culture system for 80 hours. The highest cell dry weight (CDW) of bacteria reached to 5.3 g/l observed after 60 h of operation, which was corresponding to 62.6 % of PHB. The results suggested the efficiency of the system for production of PHB in large scale. Many solvent systems were conducted to evaluate the best solvent for PHB extraction from bacterial cell in the second step. Chloroform– hypochlorite dispersion extraction was followed by that extraction with chloroform showed the best solvent system for yielding of PHB. Relatively; it has the high rate with 63% yield of PHB. Characterization study of PHB was the last step. PHB were included chemical, physical; mechanical and solubility properties of the produced PHB have been characterized by many analyses techniques, comprising Gas Chromatography Mass Spectrometry (GC-MS), Fourier-transform Infrared Spectroscopy (FTIR), X-Ray diffraction (X-Ray) and melting point. PHB was a yellowish white crystal, soluble in chloroform and other chlorinated hydrocarbons like dichloromethane, dichlorethane and chloropropane. X-Ray diffraction (XRD) study was carried out to check if the polymer had a crystalline or amorphous structure. The increased intensity of peaks showed that the polymer had more organized packed crystalline structure. The results of the GC-MS recorded that there were 9 different active peaks predictive. The two major compounds that were produced by P. aeruginosa Dw7 were then identified as undecanoic acid which eluted at 15.4 min and tridecanoic acid at 17.3 min with relative abundance of 100% and 33.14% respectively. The characteristic FTIR peaks for PHB indicated that the most prominent marker band for the identification of PHB is the ester carbonyl band at C=O, OH and CO .

Research paper thumbnail of Biological Treatment of Organ Chlorinated Pesticide Using Local Bacterial Isolates

International Journal for Sciences and Technology, 2013

The aim of this research was to isolate microbial isolates with ability of growing in medium with... more The aim of this research was to isolate microbial isolates with ability of growing in medium with presence of added pesticide and may therefore be used for bioremiation of pesticides contaminated sites (Soil and water). A bacterial groups which capable of degradation of chlorinated organic pesticides was isolated from many agricultural soil and contaminated water. After purification the strains were assessed in order to discover their ability to degrade (COP) (α propachlor) with concentration of 25mg/L as carbon source in mineral medium and in rich medium. However, the best three pure strains were able to grow in M.M includes (COP) without enrichment. This group which was composed of three isolates characterized based on their morphological and biochemical characteristic. The isolates were presumptively identified as rhodococcus spp. and Streptomyces albus. Using growth curve as a parameter of (COP) compounds, optimum conditions (Pesticide concentration, temperature, and time) of selected degrading bacterial strains were studied. Results indicated that the optimal temperature was 37°C for all isolates, with best growth at 100mg/L of propachlor by str. albus, in addition of its efficiency to degrade the compound during 48 hr at incubation.

Research paper thumbnail of The inhibitory effect of silver nanoparticles on someespecially bacteria microorganisms, Pesudomonas aeruginosa, Esherichia coli and Staphylococcus aureus

2ND INTERNATIONAL CONFERENCE ON MATERIALS ENGINEERING & SCIENCE (IConMEAS 2019)

Research paper thumbnail of Bioplastic (Poly -3-HYDROXYBUTYRATE) Production by Local Pseudomonas Aeruginosa Isolates Utilizing Waste Cooking Oil

World Journal of Pharmaceutical Research

Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes t... more Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes to the deterioration of the environment and creating severs problems. Synthetic plastic poses threat to the environment, because it is non-biodegradable are now accumulate in the environment at great millions of tons per year.

Research paper thumbnail of Activity Assay of Bacterial Mixed Culture in Biological Treatment of Organochloropesticides

World Journal of Pharmaceutical Research

Research paper thumbnail of Biological Treatment of Organ Chlorinated Pesticide Using Local Bacterial Isolates

International Journal for Sciences and Technology, 2013

The aim of this research was to isolate microbial isolates with ability of growing in medium with... more The aim of this research was to isolate microbial isolates with ability of growing in medium with presence of added pesticide and may therefore be used for bioremiation of pesticides contaminated sites (Soil and water). A bacterial groups which capable of degradation of chlorinated organic pesticides was isolated from many agricultural soil and contaminated water. After purification the strains were assessed in order to discover their ability to degrade (COP) (α propachlor) with concentration of 25mg/L as carbon source in mineral medium and in rich medium. However, the best three pure strains were able to grow in M.M includes (COP) without enrichment. This group which was composed of three isolates characterized based on their morphological and biochemical characteristic. The isolates were presumptively identified as rhodococcus spp. and Streptomyces albus. Using growth curve as a parameter of (COP) compounds, optimum conditions (Pesticide concentration, temperature, and time) of selected degrading bacterial strains were studied. Results indicated that the optimal temperature was 37°C for all isolates, with best growth at 100mg/L of propachlor by str. albus, in addition of its efficiency to degrade the compound during 48 hr at incubation.

Research paper thumbnail of Biosynthesis of Medium Chain Length Poly-β-hydroxybutyrate by Pseudomonas aeruginosa Dw7

Polyhydroxya lkanoates (PHAs), are a promising family of bio-based polymers, which considered to ... more Polyhydroxya lkanoates (PHAs), are a promising family of bio-based polymers, which considered to be alternatives to traditional petroleum-based plastics. Poly-β-hydroxybutyrate (PHB) is the most known degradable biopolymers, produced by bacterial genera. It is generally accepted that PHB can be used instead of plastic to solve one of the greatest problems facing the environment. Pseudomonas aeruginosa Dw7 was grown on mineral salt medium supplemented with waste cooking of corn oil for the synthesis of a medium chain-PHB. This study is divided into three steps, at first fermentation of P.seudomonas aeruginosa Dw7, which was carried out aerobically at optimum temperature of 30˚C and 500 rpm of agitation speed. Lab scale bioreactor (5L) operated as a batch culture system for 80 hours. The highest cell dry weight (CDW) of bacteria reached to 5.3 g/l observed after 60 h of operation, which was corresponding to 62.6 % of PHB. The results suggested the efficiency of the system for production of PHB in large scale. Many solvent systems were conducted to evaluate the best solvent for PHB extraction from bacterial cell in the second step. Chloroform– hypochlorite dispersion extraction was followed by that extraction with chloroform showed the best solvent system for yielding of PHB. Relatively; it has the high rate with 63% yield of PHB. Characterization study of PHB was the last step. PHB were included chemical, physical; mechanical and solubility properties of the produced PHB have been characterized by many analyses techniques, comprising Gas Chromatography Mass Spectrometry (GC-MS), Fourier-transform Infrared Spectroscopy (FTIR), X-Ray diffraction (X-Ray) and melting point. PHB was a yellowish white crystal, soluble in chloroform and other chlorinated hydrocarbons like dichloromethane, dichlorethane and chloropropane. X-Ray diffraction (XRD) study was carried out to check if the polymer had a crystalline or amorphous structure. The increased intensity of peaks showed that the polymer had more organized packed crystalline structure. The results of the GC-MS recorded that there were 9 different active peaks predictive. The two major compounds that were produced by P. aeruginosa Dw7 were then identified as undecanoic acid which eluted at 15.4 min and tridecanoic acid at 17.3 min with relative abundance of 100% and 33.14% respectively. The characteristic FTIR peaks for PHB indicated that the most prominent marker band for the identification of PHB is the ester carbonyl band at C=O, OH and CO .