batool hassan Al-ghurabi | University of Baghdad (original) (raw)

Papers by batool hassan Al-ghurabi

The Egyptian Journal of Hospital Medicine

Background: Despite the fact that fluoride-based remineralization technologies have dominated pre... more Background: Despite the fact that fluoride-based remineralization technologies have dominated preventive dentistry for the past century, but new and innovative nanomaterials approaches are transforming the industry. A variety of unique nanomaterials are now easily available to assist remineralization of dental enamel surface. Objective: investigate the effect of indium oxide nanoparticles application in comparison to de-ionized water and sodium fluoride on human tooth enamel by assessing the morphological changes in enamel ultra-structure. Using a field emission scanning electron microscope, x-ray diffraction, and ultraviolet visible absorption to evaluate the physical characteristics of indium oxide nanoparticles created by laser ablation in liquid. Method: Teeth samples were seventy-two divided randomly into two phases; each phase consists of study and treated groups with indium oxide nanoparticles, control positive treated with sodium fluoride, and control negative with deionized water. Each group was consisted of twelve teeth, from each group two teeth for scanning electron microscopy and the rests were for energy dispersion spectroscopy. Result: result showed change in chemical composition of dental enamel was recorded for both preventive and treatment groups after nanoparticles application and this was supported by energy dispersive spectroscopy and scanning electron microscope. Conclusion: Laser prepared indium oxide nanoparticles colloidal suspensions were found to be effective remineralizing agent to dental enamel and thus can be considered as an alternative to sodium fluoride mouth wash.

International journal of health sciences

Periodontitis is a complex infectious disease that affects a wide range of people and has a lot o... more Periodontitis is a complex infectious disease that affects a wide range of people and has a lot of risk factors. Vitamin D increases innate immunity by regulating the generation of antimicrobial peptides and cytokine response, which may minimize the risk of infection through a variety of methods. Antimicrobial peptides defend the host from a variety of pathogens. This study was conducted to determine the vitamin D levels in patients and investigate how vitamin D affects the levels of antimicrobial peptides. The University of Baghdad's Department of Periodontics, College of Dentistry, and clinic recruited fifty new patients. For each patient, periodontal parameters were assessed. In order to estimate the level of vitamin D and antimicrobial peptides (cathelicidins and human beta defensins-1), blood samples from all patients were taken. The finding revealed that serum vitamin D deficiency is associated with decreased levels of cathelicidins and human beta defensins-1 in serum of p...

Journal of Baghdad College of Dentistry

Background:SARS-CoV-2 infection has caused a global pandemic that continues to negatively impact ... more Background:SARS-CoV-2 infection has caused a global pandemic that continues to negatively impact human health. A large group of microbial domains including bacteria co-evolved and interacted in complex molecular pathogenesis along with SARS-CoV-2. Evidence suggests that periodontal disease bacteria are involved in COVID-19, and are associated with chronic inflammatory systemic diseases. This study was performed to investigate the association between bacterial loads of Porphyromonas gingivalis and pathogenesis of SARS-CoV-2 infection. Fifty patients with confirmed COVID-19 by reverse transcriptase-polymerase chain reaction, their age ranges between 20-76 years, and 35 healthy volunteers (matched accordingly with age and sex to the patients) participated in this case control study. Oral hygiene status was determined by the simplified oral hygiene index. Blood and saliva samples were obtained from patients and controls, Porphyromonas gingivalis quantification from extracted DNA of bloo...

Journal of Emergency Medicine, Trauma and Acute Care

Journal of Research in Medical and Dental Science, 2021

Journal of the Faculty of Medicine, 2010

Background: It is well known that the low level laser irradiation has act on immune system cells ... more Background: It is well known that the low level laser irradiation has act on immune system cells in a number of ways, one of them includes increasing phagocytic activity of irradiated cells. This study was established to shed light on the possible effect of laser irradiation on phagocytic activity of polymorphoneutrophils. Subjects and methods: Fresh blood samples were obtained from twenty healthy volunteers for phagocytosis assay. The polymorphoneutrophils were isolated from blood and examined their phagocytic capacity befor and after exposure to laser irradiation. Results: The present study revealed a significant increase in the mean percentage of phagocytosis after exposure to diode laser of wave length (632) nm (red), which was further increased by increasing the time of exposure. Conclusion: The current study suggests that low level of laser can increase the phagocytic activity of polymorphoneutrophils, and this increase propotional to increaseing the time of exposure.

Journal of the Faculty of Medicine, Oct 1, 2009

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in the pathogenesis of many solid cancers.This study aimed to estimate serum levels of IL-2 and IL-4, and to shed light on the correlation of these interleukins with progression of breast cancer. Patients and Methods: The study included 80 women, it comprised of 45 breast cancer patients, 12 patients with benign breast lesions and 23 apparently healthy controls. ELISA method has been used for estimation the level of IL-2 and IL-4 in serum of three studied groups. Results: This study showed elevation of IL-4 level in the sera of breast cancer patients with significant differences between breast cancer and controls (p<0.001), this elevation was correlated with progression of the tumor. In addition, the elevation of serum level of IL-4 was found to be inversely related to ER and PR expression (P=<0.05). On the other hand, there was a significant decrease in the median of serum level of IL-2 in patients as compared with control groups. Conclusions: There was association between elevated serum level of IL-4 and breast cancer and this elevation was correlated with advanced stage of disease. In addition, there was no association between the statistical significant decrease of IL-2 serum level and the advanced stage of breast cancer.

Journal of the Faculty of Medicine Baghdad, 2010

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in pathogenesis of many solid cancer. This study aimed to shed light on the possible role of IL-6 in pathogenesis of CRC and its relationship with serum CEA level. Patients and methods: This study covered 50subjects. It comprised of 30 patients with CRC, compared with 20 healthy normal controls. Serum IL-6 and CEA analysis were performed by ELISA. Results: This study revealed a significant elevation in serum IL-6 level among CRC patients (16.2 pg/ml) in comparison to that of healthy control (4.8 pg/ml) (p<0.001). As well as an interesting significant increase of mean serum CEA level in CRC patients (7.5± 6.62 ng/ml) as compared to healthy control (2.4±0.90 ng/ml). On the other hand there was strong linear correlation between serum CEA level and serum IL-6 level p<0.001. Conclusion: There are a significant correlation between the elevation o...

Journal of the Faculty of Medicine Baghdad, 2010

Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The gene... more Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region seem to influence susceptibility to certain diseases. Patients and methods: Polymerase chain reaction-Sequence Specific Primers PCR-SSP is the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls. Results: An increased frequency of HLA-DR3, DR4 and DR7 was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively, while DR*0211 (DR2) may be formed the basis for protection against the disease. HLA-DQ on the other hand, yielded on association in Iraqi patients with AIH. Conclusions: This finding demonstrated that HLA-DR3, DR4 and DR7 might play a role in AIH susceptibility.

Background: It is well known that the low level laser irradiation has act on immune system cells ... more Background: It is well known that the low level laser irradiation has act on immune system cells in a number of ways, one of them includes increasing phagocytic activity of irradiated cells. This study was established to shed light on the possible effect of laser irradiation on phagocytic activity of polymorphoneutrophils. Subjects and methods: Fresh blood samples were obtained from twenty healthy volunteers for phagocytosis assay. The polymorphoneutrophils were isolated from blood and examined their phagocytic capacity befor and after exposure to laser irradiation. Results: The present study revealed a significant increase in the mean percentage of phagocytosis after exposure to diode laser of wave length (632) nm (red), which was further increased by increasing the time of exposure. Conclusion: The current study suggests that low level of laser can increase the phagocytic activity of polymorphoneutrophils, and this increase propotional to increaseing the time of exposure.

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in the pathogenesis of many solid cancers.This study aimed to estimate serum levels of IL-2 and IL-4, and to shed light on the correlation of these interleukins with progression of breast cancer. Patients and Methods: The study included 80 women, it comprised of 45 breast cancer patients, 12 patients with benign breast lesions and 23 apparently healthy controls. ELISA method has been used for estimation the level of IL-2 and IL-4 in serum of three studied groups. Results: This study showed elevation of IL-4 level in the sera of breast cancer patients with significant differences between breast cancer and controls (p<0.001), this elevation was correlated with progression of the tumor. In addition, the elevation of serum level of IL-4 was found to be inversely related to ER and PR expression (P=<0.05). On the other hand, there was a significa...

Annals of Tropical Medicine and Public Health, 2021

Background: Periodontitis is a multifactorial disease that involves microorganism and host respon... more Background: Periodontitis is a multifactorial disease that involves microorganism and host response caus ing periodontal tissue damages. MiRNAs is one of the epigenetic factors deregulation and many of them can contribute to the periodontitis pathogenesis. Thecurrent study aimed to investigate the level of miRNA-146a and IL-17A in saliva of chronic periodontitis patients and controls.This case-control study was performed on (40) chronic periodontitis patients and (40) healthy individuals as controls. Periodontal parameters used in the present study included PLI, GI, PPD and CAL. Saliva samples were taken from all subjects . ELISA was carried out to estimate the levels of IL-17, while miRNA-146a was determined by RTqPCR. The current results found a significant elevation in median salivary levels of IL-17 in patients group when compared with the control group (P˂0.05). Moreover, IL-17 showed ahighly significant positive correlation with PLI, PPD&CAL. However, miRNA-146a revealed a sig...

Hydatidosis is a parasitic disease caused by infection with larval stage of cestode worm Echinoco... more Hydatidosis is a parasitic disease caused by infection with larval stage of cestode worm Echinococcus granulosus ,it is a worle_wide disease results in serious health and financial loss. Iraq considered as an endemic region for this disease. the association of HLA –alleles frequency with the occurrence of various diseases were studied . and significant associations were found, no sufficient studies on hydatidosis and possible role of HLA typing and the immune status of the patients and association of them with cyst characteristics ,and cytokines profile and their effect on production of immunoglobulin . blood samples were collected for extraction of DNA and amplification by (PCR-SSO) and HLA typing by Automatic Line Probe Assay (Auto-Lipa) for HLA typing of both patients and healthy control groups, to study the association of certain HLA alleles. With occurrence of Hydatid disease. The present study reported increase in frequency of HLA-A*0273 in hydatid patients compare to healthy ...

Journal of Natural Product and Plant Resources, 2017

Hydatid disease or cystic echinococcosis is a parasitic infection caused by the larval stage of t... more Hydatid disease or cystic echinococcosis is a parasitic infection caused by the larval stage of tape worm is one of the most significant zoonosis all around the world. In addition to environmental factors, genetic constitution of hosts seems to play a crucial role in acquiring the infection and developing disease. This study was carried out to investigate the association of HLA-class class I (A, B) with hydatid disease by genotyping in Iraqi patients, as well as to provide information about genotypes that confer susceptibility or resistance to develop the disease. Thirty patients with hydatid disease their age range (16-57) years and twenty healthy controls their ages were matched with the patients were enrolled in this study. Blood was collected from patients and controls, DNA was extracted from blood samples, and then HLA-Class I genotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). The present findings showed that HLA-A *0273 al...

International Journal of Medical Research and Health Sciences, 2017

Background: Mobiles have become one of the most indispensable accessories of profession and socia... more Background: Mobiles have become one of the most indispensable accessories of profession and social life. The mobiles make life easier, but they pose a number of new hazards also. Objectives: The principle aim of this study was to evaluate the microbial contamination of mobile phones belonging to dentists in College of Dentistry in Baghdad University. Materials and Methods: 35 dentists (20 female and 15 male) were enrolled in this study. Sampling was taken from each participant’s mobile by using moist sterile swab impregnated by normal saline for microbial analyses. Results: The findings of this study revealed that the growth of microorganisms has found in all samples taken from the mobile phones of dentists. The most common microorganisms detected were Staphylococcus epidermidis, Micrococcus spp., Candida albicans and Aspergillus niger. On the other hand, there was no significant difference (P>0.05) in the microorganisms isolated and their percentage frequency of occurrence betwe...

Endometriosis is a chronic gynecological disease manifested by the occurrence of ectopic foci of ... more Endometriosis is a chronic gynecological disease manifested by the occurrence of ectopic foci of endometrial tissue in the pelvic cavity and/or ovary. Etiopathogenesis of the disease is still poorly understood but there is a growing bulk of evidence that genetic factors and immunological abnormalities play a role in this disease. This study was performed to investigate the association of human leukocyte antigens class II genotypes (HLA-DR and DQ) with the susceptibility to endometriosis. Fifty female patients with endometriosis their age range (19 – 46) years and 30 females as control their ages were matched with the patients were enrolled in this study. Blood was collected from patients and controls, DNA was extracted from blood samples, and then HLAgenotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). The present findings showed that DRB1*0307, DRB1*0701 and DQB1*0301 alleles were found with highly significant frequencies among p...

Background: Quantitation of serum immunoglobulins (IgG, IgA and IgM) provides useful information ... more Background: Quantitation of serum immunoglobulins (IgG, IgA and IgM) provides useful information for the evaluation of certain cancers. Objectives:to estimate serum immunoglobulins level before and after surgery, and to shed light on the correlation of immunoglobulins with progression of CRC. Patients and Methodes. By single radial immune diffusion method IgG, IgA, IgM were estimated in 100 CRC patients preoperatively, and in 20 patients postoperatively compared with 35 patients control with ulcerative colitis (UC) and 50 healthy control. Results: The study showed significantly increased the serum IgM level in patients group preoperatively (p<0.001) compared with control group. On the other hand, no significant differences were observed in respect to the mean of IgG, IgA, and IgM after surgical excision of the tumor in 20 patients. J Fac Med Baghdad 2007; Vol. 49, No.2 Received Sep. 2006 Accepted Jan. 2007

Background: It is essential that peri-implant and periodontal sites are studied to contribute com... more Background: It is essential that peri-implant and periodontal sites are studied to contribute comparison data for investigations that are dealing with diseased sites. The objective of this study was to compare the expression of host-derived markers interleukin-1β (IL-1β) and myeloperoxidase (MPO) in peri-implant sulcular fluid (PISF) with the gingival crevicular fluid (GCF) of contralateral natural teeth and to investigate the anti-inflammatory effect of Aloe vera gel on these biomarkers. Materials and Methods: 20 subjects aged 2545 year who have one clinically stable titanium implant carrying a full ceramic crown and one contralateral natural tooth were enrolled in a split-mouth study.Clinical parameters were measured including Plaque index (Pl I), ,Gingival Index (GI), Probing depth (PD). PISF and GCF were collected and the levels of interleukin-1β and myeloperoxidase were determined usingELISA technique at baseline and 2 weeks after application of Aloe vera gel. Results: IL-1β le...

Iraqi postgraduate Medical Journal, 2013

BACKGROUND: Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of... more BACKGROUND: Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus. OBJECTIVE: This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus. SUBJECTS AND METHODS: Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. RESULTS: The present study revealed significantly high frequency of DQB1*0101 and DQB1*0201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A*3301, B*0826, DRB1*0701, *1101 and H...

Iraqi journal of science, 2010

The Egyptian Journal of Hospital Medicine

Background: Despite the fact that fluoride-based remineralization technologies have dominated pre... more Background: Despite the fact that fluoride-based remineralization technologies have dominated preventive dentistry for the past century, but new and innovative nanomaterials approaches are transforming the industry. A variety of unique nanomaterials are now easily available to assist remineralization of dental enamel surface. Objective: investigate the effect of indium oxide nanoparticles application in comparison to de-ionized water and sodium fluoride on human tooth enamel by assessing the morphological changes in enamel ultra-structure. Using a field emission scanning electron microscope, x-ray diffraction, and ultraviolet visible absorption to evaluate the physical characteristics of indium oxide nanoparticles created by laser ablation in liquid. Method: Teeth samples were seventy-two divided randomly into two phases; each phase consists of study and treated groups with indium oxide nanoparticles, control positive treated with sodium fluoride, and control negative with deionized water. Each group was consisted of twelve teeth, from each group two teeth for scanning electron microscopy and the rests were for energy dispersion spectroscopy. Result: result showed change in chemical composition of dental enamel was recorded for both preventive and treatment groups after nanoparticles application and this was supported by energy dispersive spectroscopy and scanning electron microscope. Conclusion: Laser prepared indium oxide nanoparticles colloidal suspensions were found to be effective remineralizing agent to dental enamel and thus can be considered as an alternative to sodium fluoride mouth wash.

International journal of health sciences

Periodontitis is a complex infectious disease that affects a wide range of people and has a lot o... more Periodontitis is a complex infectious disease that affects a wide range of people and has a lot of risk factors. Vitamin D increases innate immunity by regulating the generation of antimicrobial peptides and cytokine response, which may minimize the risk of infection through a variety of methods. Antimicrobial peptides defend the host from a variety of pathogens. This study was conducted to determine the vitamin D levels in patients and investigate how vitamin D affects the levels of antimicrobial peptides. The University of Baghdad's Department of Periodontics, College of Dentistry, and clinic recruited fifty new patients. For each patient, periodontal parameters were assessed. In order to estimate the level of vitamin D and antimicrobial peptides (cathelicidins and human beta defensins-1), blood samples from all patients were taken. The finding revealed that serum vitamin D deficiency is associated with decreased levels of cathelicidins and human beta defensins-1 in serum of p...

Journal of Baghdad College of Dentistry

Background:SARS-CoV-2 infection has caused a global pandemic that continues to negatively impact ... more Background:SARS-CoV-2 infection has caused a global pandemic that continues to negatively impact human health. A large group of microbial domains including bacteria co-evolved and interacted in complex molecular pathogenesis along with SARS-CoV-2. Evidence suggests that periodontal disease bacteria are involved in COVID-19, and are associated with chronic inflammatory systemic diseases. This study was performed to investigate the association between bacterial loads of Porphyromonas gingivalis and pathogenesis of SARS-CoV-2 infection. Fifty patients with confirmed COVID-19 by reverse transcriptase-polymerase chain reaction, their age ranges between 20-76 years, and 35 healthy volunteers (matched accordingly with age and sex to the patients) participated in this case control study. Oral hygiene status was determined by the simplified oral hygiene index. Blood and saliva samples were obtained from patients and controls, Porphyromonas gingivalis quantification from extracted DNA of bloo...

Journal of Emergency Medicine, Trauma and Acute Care

Journal of Research in Medical and Dental Science, 2021

Journal of the Faculty of Medicine, 2010

Background: It is well known that the low level laser irradiation has act on immune system cells ... more Background: It is well known that the low level laser irradiation has act on immune system cells in a number of ways, one of them includes increasing phagocytic activity of irradiated cells. This study was established to shed light on the possible effect of laser irradiation on phagocytic activity of polymorphoneutrophils. Subjects and methods: Fresh blood samples were obtained from twenty healthy volunteers for phagocytosis assay. The polymorphoneutrophils were isolated from blood and examined their phagocytic capacity befor and after exposure to laser irradiation. Results: The present study revealed a significant increase in the mean percentage of phagocytosis after exposure to diode laser of wave length (632) nm (red), which was further increased by increasing the time of exposure. Conclusion: The current study suggests that low level of laser can increase the phagocytic activity of polymorphoneutrophils, and this increase propotional to increaseing the time of exposure.

Journal of the Faculty of Medicine, Oct 1, 2009

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in the pathogenesis of many solid cancers.This study aimed to estimate serum levels of IL-2 and IL-4, and to shed light on the correlation of these interleukins with progression of breast cancer. Patients and Methods: The study included 80 women, it comprised of 45 breast cancer patients, 12 patients with benign breast lesions and 23 apparently healthy controls. ELISA method has been used for estimation the level of IL-2 and IL-4 in serum of three studied groups. Results: This study showed elevation of IL-4 level in the sera of breast cancer patients with significant differences between breast cancer and controls (p<0.001), this elevation was correlated with progression of the tumor. In addition, the elevation of serum level of IL-4 was found to be inversely related to ER and PR expression (P=<0.05). On the other hand, there was a significant decrease in the median of serum level of IL-2 in patients as compared with control groups. Conclusions: There was association between elevated serum level of IL-4 and breast cancer and this elevation was correlated with advanced stage of disease. In addition, there was no association between the statistical significant decrease of IL-2 serum level and the advanced stage of breast cancer.

Journal of the Faculty of Medicine Baghdad, 2010

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in pathogenesis of many solid cancer. This study aimed to shed light on the possible role of IL-6 in pathogenesis of CRC and its relationship with serum CEA level. Patients and methods: This study covered 50subjects. It comprised of 30 patients with CRC, compared with 20 healthy normal controls. Serum IL-6 and CEA analysis were performed by ELISA. Results: This study revealed a significant elevation in serum IL-6 level among CRC patients (16.2 pg/ml) in comparison to that of healthy control (4.8 pg/ml) (p<0.001). As well as an interesting significant increase of mean serum CEA level in CRC patients (7.5± 6.62 ng/ml) as compared to healthy control (2.4±0.90 ng/ml). On the other hand there was strong linear correlation between serum CEA level and serum IL-6 level p<0.001. Conclusion: There are a significant correlation between the elevation o...

Journal of the Faculty of Medicine Baghdad, 2010

Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The gene... more Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region seem to influence susceptibility to certain diseases. Patients and methods: Polymerase chain reaction-Sequence Specific Primers PCR-SSP is the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls. Results: An increased frequency of HLA-DR3, DR4 and DR7 was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively, while DR*0211 (DR2) may be formed the basis for protection against the disease. HLA-DQ on the other hand, yielded on association in Iraqi patients with AIH. Conclusions: This finding demonstrated that HLA-DR3, DR4 and DR7 might play a role in AIH susceptibility.

Background: It is well known that the low level laser irradiation has act on immune system cells ... more Background: It is well known that the low level laser irradiation has act on immune system cells in a number of ways, one of them includes increasing phagocytic activity of irradiated cells. This study was established to shed light on the possible effect of laser irradiation on phagocytic activity of polymorphoneutrophils. Subjects and methods: Fresh blood samples were obtained from twenty healthy volunteers for phagocytosis assay. The polymorphoneutrophils were isolated from blood and examined their phagocytic capacity befor and after exposure to laser irradiation. Results: The present study revealed a significant increase in the mean percentage of phagocytosis after exposure to diode laser of wave length (632) nm (red), which was further increased by increasing the time of exposure. Conclusion: The current study suggests that low level of laser can increase the phagocytic activity of polymorphoneutrophils, and this increase propotional to increaseing the time of exposure.

Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well ... more Background: The role of cytokines in cancer immunity and carcinogenesis in general has been well established, which play an important role in the pathogenesis of many solid cancers.This study aimed to estimate serum levels of IL-2 and IL-4, and to shed light on the correlation of these interleukins with progression of breast cancer. Patients and Methods: The study included 80 women, it comprised of 45 breast cancer patients, 12 patients with benign breast lesions and 23 apparently healthy controls. ELISA method has been used for estimation the level of IL-2 and IL-4 in serum of three studied groups. Results: This study showed elevation of IL-4 level in the sera of breast cancer patients with significant differences between breast cancer and controls (p<0.001), this elevation was correlated with progression of the tumor. In addition, the elevation of serum level of IL-4 was found to be inversely related to ER and PR expression (P=<0.05). On the other hand, there was a significa...

Annals of Tropical Medicine and Public Health, 2021

Background: Periodontitis is a multifactorial disease that involves microorganism and host respon... more Background: Periodontitis is a multifactorial disease that involves microorganism and host response caus ing periodontal tissue damages. MiRNAs is one of the epigenetic factors deregulation and many of them can contribute to the periodontitis pathogenesis. Thecurrent study aimed to investigate the level of miRNA-146a and IL-17A in saliva of chronic periodontitis patients and controls.This case-control study was performed on (40) chronic periodontitis patients and (40) healthy individuals as controls. Periodontal parameters used in the present study included PLI, GI, PPD and CAL. Saliva samples were taken from all subjects . ELISA was carried out to estimate the levels of IL-17, while miRNA-146a was determined by RTqPCR. The current results found a significant elevation in median salivary levels of IL-17 in patients group when compared with the control group (P˂0.05). Moreover, IL-17 showed ahighly significant positive correlation with PLI, PPD&CAL. However, miRNA-146a revealed a sig...

Hydatidosis is a parasitic disease caused by infection with larval stage of cestode worm Echinoco... more Hydatidosis is a parasitic disease caused by infection with larval stage of cestode worm Echinococcus granulosus ,it is a worle_wide disease results in serious health and financial loss. Iraq considered as an endemic region for this disease. the association of HLA –alleles frequency with the occurrence of various diseases were studied . and significant associations were found, no sufficient studies on hydatidosis and possible role of HLA typing and the immune status of the patients and association of them with cyst characteristics ,and cytokines profile and their effect on production of immunoglobulin . blood samples were collected for extraction of DNA and amplification by (PCR-SSO) and HLA typing by Automatic Line Probe Assay (Auto-Lipa) for HLA typing of both patients and healthy control groups, to study the association of certain HLA alleles. With occurrence of Hydatid disease. The present study reported increase in frequency of HLA-A*0273 in hydatid patients compare to healthy ...

Journal of Natural Product and Plant Resources, 2017

Hydatid disease or cystic echinococcosis is a parasitic infection caused by the larval stage of t... more Hydatid disease or cystic echinococcosis is a parasitic infection caused by the larval stage of tape worm is one of the most significant zoonosis all around the world. In addition to environmental factors, genetic constitution of hosts seems to play a crucial role in acquiring the infection and developing disease. This study was carried out to investigate the association of HLA-class class I (A, B) with hydatid disease by genotyping in Iraqi patients, as well as to provide information about genotypes that confer susceptibility or resistance to develop the disease. Thirty patients with hydatid disease their age range (16-57) years and twenty healthy controls their ages were matched with the patients were enrolled in this study. Blood was collected from patients and controls, DNA was extracted from blood samples, and then HLA-Class I genotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). The present findings showed that HLA-A *0273 al...

International Journal of Medical Research and Health Sciences, 2017

Background: Mobiles have become one of the most indispensable accessories of profession and socia... more Background: Mobiles have become one of the most indispensable accessories of profession and social life. The mobiles make life easier, but they pose a number of new hazards also. Objectives: The principle aim of this study was to evaluate the microbial contamination of mobile phones belonging to dentists in College of Dentistry in Baghdad University. Materials and Methods: 35 dentists (20 female and 15 male) were enrolled in this study. Sampling was taken from each participant’s mobile by using moist sterile swab impregnated by normal saline for microbial analyses. Results: The findings of this study revealed that the growth of microorganisms has found in all samples taken from the mobile phones of dentists. The most common microorganisms detected were Staphylococcus epidermidis, Micrococcus spp., Candida albicans and Aspergillus niger. On the other hand, there was no significant difference (P>0.05) in the microorganisms isolated and their percentage frequency of occurrence betwe...

Endometriosis is a chronic gynecological disease manifested by the occurrence of ectopic foci of ... more Endometriosis is a chronic gynecological disease manifested by the occurrence of ectopic foci of endometrial tissue in the pelvic cavity and/or ovary. Etiopathogenesis of the disease is still poorly understood but there is a growing bulk of evidence that genetic factors and immunological abnormalities play a role in this disease. This study was performed to investigate the association of human leukocyte antigens class II genotypes (HLA-DR and DQ) with the susceptibility to endometriosis. Fifty female patients with endometriosis their age range (19 – 46) years and 30 females as control their ages were matched with the patients were enrolled in this study. Blood was collected from patients and controls, DNA was extracted from blood samples, and then HLAgenotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). The present findings showed that DRB1*0307, DRB1*0701 and DQB1*0301 alleles were found with highly significant frequencies among p...

Background: Quantitation of serum immunoglobulins (IgG, IgA and IgM) provides useful information ... more Background: Quantitation of serum immunoglobulins (IgG, IgA and IgM) provides useful information for the evaluation of certain cancers. Objectives:to estimate serum immunoglobulins level before and after surgery, and to shed light on the correlation of immunoglobulins with progression of CRC. Patients and Methodes. By single radial immune diffusion method IgG, IgA, IgM were estimated in 100 CRC patients preoperatively, and in 20 patients postoperatively compared with 35 patients control with ulcerative colitis (UC) and 50 healthy control. Results: The study showed significantly increased the serum IgM level in patients group preoperatively (p<0.001) compared with control group. On the other hand, no significant differences were observed in respect to the mean of IgG, IgA, and IgM after surgical excision of the tumor in 20 patients. J Fac Med Baghdad 2007; Vol. 49, No.2 Received Sep. 2006 Accepted Jan. 2007

Background: It is essential that peri-implant and periodontal sites are studied to contribute com... more Background: It is essential that peri-implant and periodontal sites are studied to contribute comparison data for investigations that are dealing with diseased sites. The objective of this study was to compare the expression of host-derived markers interleukin-1β (IL-1β) and myeloperoxidase (MPO) in peri-implant sulcular fluid (PISF) with the gingival crevicular fluid (GCF) of contralateral natural teeth and to investigate the anti-inflammatory effect of Aloe vera gel on these biomarkers. Materials and Methods: 20 subjects aged 2545 year who have one clinically stable titanium implant carrying a full ceramic crown and one contralateral natural tooth were enrolled in a split-mouth study.Clinical parameters were measured including Plaque index (Pl I), ,Gingival Index (GI), Probing depth (PD). PISF and GCF were collected and the levels of interleukin-1β and myeloperoxidase were determined usingELISA technique at baseline and 2 weeks after application of Aloe vera gel. Results: IL-1β le...

Iraqi postgraduate Medical Journal, 2013

BACKGROUND: Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of... more BACKGROUND: Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus. OBJECTIVE: This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus. SUBJECTS AND METHODS: Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. RESULTS: The present study revealed significantly high frequency of DQB1*0101 and DQB1*0201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A*3301, B*0826, DRB1*0701, *1101 and H...

Iraqi journal of science, 2010