Dr. Sarada D Tetali | University of Hyderabad (original) (raw)

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Papers by Dr. Sarada D Tetali

Research paper thumbnail of Metabolomics of Withania somnifera (L.) Dunal: Advances and applications

Journal of Ethnopharmacology

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Research paper thumbnail of Interactions of different lipoproteins with supported phospholipid raft membrane (SPRM) patterns to understand similar in-vivo processes

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Research paper thumbnail of Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells

Clinical Hemorheology and Microcirculation

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Research paper thumbnail of Dry leaf extracts of Tinospora cordifolia (Willd.) Miers attenuate oxidative stress and inflammatory condition in human monocytic (THP-1) cells

Phytomedicine

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Research paper thumbnail of Biochemical properties of a bacterially-expressed Bowman-Birk inhibitor from Rhynchosia sublobata (Schumach.) Meikle seeds and its activity against gut proteases of Achaea janata

Phytochemistry

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Research paper thumbnail of Terpenes and isoprenoids: a wealth of compounds for global use

Planta

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Research paper thumbnail of Highly Sensitive HPLC Method for Estimation of Total or Individual Curcuminoids in <i>Curcuma</i> Cultivars and Commercial Turmeric Powders

Current Science

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Research paper thumbnail of Metabolomics of Medicinal Plants - A Versatile Tool for Standardization of Herbal Products and Quality Evaluation of Ayurvedic Formulations

Current Science, 2016

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Research paper thumbnail of Influence of nanoporous poly (ether imide) particle extracts on human aortic endothelial cells (HAECs)

Clinical Hemorheology and Microcirculation, 2016

Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal fa... more Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal failure patients stimulate proinflammatory effects, and consequently kidney and cardiovascular diseases. Low clearance rate of these uremic toxins from the blood of uremic patients by conventional techniques like hemodialysis is due to their strong covalent albumin binding (greater than 95%) and hydrophobic nature, which led to alternatives like usage of hydrophobic adsorber&amp;amp;#39;s in removing these toxins from the plasma of kidney patients. Polymers like polyethylene, polyurethane, polymethylmethacrylate, cellophane and polytetrafluoroethylene were already in use as substitutes for metal devices as dialysis membranes. Among new synthetic polymers, one such ideal adsorber material are highly porous microparticles of poly(ether imide) (PEI) with diameters in the range from 50-180μm and a porosity around 88±2% prepared by a spraying and coagulation process.It is essential to make sure that these synthetic polymers should not evoke any inflammatory or apoptotic response during dialysis. Therefore in our study we evaluated in vitro effect of PEI microparticle extracts in human aortic endothelial cells (HEACs) concerning toxicity, inflammation and apoptosis. No cell toxicity was observed when HAECs were treated with PEI extracts and inflammatory/apoptotic markers were not upregulated in presence of PEI extracts. Our results ensure biocompatibility of PEI particles and further hemocompatibility of particles will be tested.

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Research paper thumbnail of Adsorption capacity of poly(ether imide) microparticles to uremic toxins

Clinical Hemorheology and Microcirculation, 2016

Uremia is a phenomenon caused by retention of uremic toxins in the plasma due to functional impai... more Uremia is a phenomenon caused by retention of uremic toxins in the plasma due to functional impairment of kidneys in the elimination of urinary waste products. Uremia is presently treated by dialysis techniques like hemofiltration, dialysis or hemodiafiltration. However, these techniques in use are more favorable towards removing hydrophilic than hydrophobic uremic toxins. Hydrophobic uremic toxins, such as hydroxy hipuric acid (OH-HPA), phenylacetic acid (PAA), indoxyl sulfate (IDS) and p-cresylsulfate (pCRS), contribute substantially to the progression of chronic kidney disease (CKD) and cardiovascular disease. Therefore, objective of the present study is to test adsorption capacity of highly porous microparticles prepared from poly(ether imide) (PEI) as an alternative technique for the removal of uremic toxins. Two types of nanoporous, spherically shaped microparticles were prepared from PEI by a spraying/coagulation process.PEI particles were packed into a preparative HPLC column to which a mixture of the four types of uremic toxins was injected and eluted with ethanol. Eluted toxins were quantified by analytical HPLC. PEI particles were able to adsorb all four toxins, with the highest affinity for PAA and pCR. IDS and OH-HPA showed a partially non-reversible binding. In summary, PEI particles are interesting candidates to be explored for future application in CKD.

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Research paper thumbnail of Effect of extracts of poly(ether imide) microparticles on cytotoxicity, ROS generation and proinflammatory effects on human monocytic (THP-1) cells

Clinical hemorheology and microcirculation, Jan 26, 2015

Current haemodialysis techniques are not capable to remove efficiently low molecular weight hydro... more Current haemodialysis techniques are not capable to remove efficiently low molecular weight hydrophobic uremic toxins from the blood of patients suffering from chronic renal failure. With respect to the hydrophobic characteristics and the high level of protein binding of these uremic toxins, hydrophobic adsorber materials might be an alternative to remove these substances from the plasma of the chronic kidney disease (CKD) patients. Here nanoporous microparticles prepared from poly(ether imide) (PEI) with an average diameter of 90±30μm and a porosity around 88±2% prepared by a spraying/coagulation process are considered as candidate adsorber materials. A prerequisite for the clinical application of such particles is their biocompatibility, which can be examined i.e. indirectly in cell culture experiments with the particles' extracts. In this work we studied the effects of aqueous extracts of PEI microparticles on the viability of THP-1 cells, a human leukemia monocytic cell line...

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Research paper thumbnail of tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size

Planta, 2003

DNA insertional mutagenesis and screening of the green alga Chlamydomonas reinhardtii was employe... more DNA insertional mutagenesis and screening of the green alga Chlamydomonas reinhardtii was employed to isolate tla1, a stable transformant having a truncated light-harvesting chlorophyll antenna size. Molecular analysis showed a single plasmid insertion into an open reading frame of the nuclear genome corresponding to a novel gene ( Tla1) that encodes a protein of 213 amino acids. Genetic analysis showed co-segregation of plasmid and tla1 phenotype. Biochemical analyses showed the tla1 mutant to be chlorophyll deficient, with a functional chlorophyll antenna size of photosystem I and photosystem II being about 50% and 65% of that of the wild type, respectively. It contained a correspondingly lower amount of light-harvesting proteins than the wild type and had lower steady-state levels of Lhcb mRNA. The tla1 strain required a higher light intensity for the saturation of photosynthesis and showed greater solar conversion efficiencies and a higher photosynthetic productivity than the wi...

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Research paper thumbnail of Testosterone-derived estradiol production by male endothelium is robust and dependent on p450 aromatase via estrogen receptor alpha

SpringerPlus, 2013

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Research paper thumbnail of Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

THE PLANT CELL ONLINE, 2004

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Research paper thumbnail of Postprandial apoE Isoform and Conformational Changes Associated with VLDL Lipolysis Products Modulate Monocyte Inflammation

PLoS ONE, 2012

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Research paper thumbnail of Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene

Planta, 2007

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Research paper thumbnail of VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation

The Journal of Lipid Research, 2010

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Research paper thumbnail of C-terminal interactions of apolipoprotein E4 respond to the postprandial state

The Journal of Lipid Research, 2006

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Research paper thumbnail of Gum resin of Boswellia serrata inhibited human monocytic (THP-1) cell activation and platelet aggregation

Journal of Ethnopharmacology, 2011

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Research paper thumbnail of Stem-bark of Terminalia arjuna attenuates human monocytic (THP-1) and aortic endothelial cell activation

Journal of Ethnopharmacology, 2013

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Research paper thumbnail of Metabolomics of Withania somnifera (L.) Dunal: Advances and applications

Journal of Ethnopharmacology

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Interactions of different lipoproteins with supported phospholipid raft membrane (SPRM) patterns to understand similar in-vivo processes

Biochimica et Biophysica Acta (BBA) - Biomembranes

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells

Clinical Hemorheology and Microcirculation

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Dry leaf extracts of Tinospora cordifolia (Willd.) Miers attenuate oxidative stress and inflammatory condition in human monocytic (THP-1) cells

Phytomedicine

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Biochemical properties of a bacterially-expressed Bowman-Birk inhibitor from Rhynchosia sublobata (Schumach.) Meikle seeds and its activity against gut proteases of Achaea janata

Phytochemistry

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Terpenes and isoprenoids: a wealth of compounds for global use

Planta

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Highly Sensitive HPLC Method for Estimation of Total or Individual Curcuminoids in <i>Curcuma</i> Cultivars and Commercial Turmeric Powders

Current Science

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Metabolomics of Medicinal Plants - A Versatile Tool for Standardization of Herbal Products and Quality Evaluation of Ayurvedic Formulations

Current Science, 2016

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Influence of nanoporous poly (ether imide) particle extracts on human aortic endothelial cells (HAECs)

Clinical Hemorheology and Microcirculation, 2016

Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal fa... more Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal failure patients stimulate proinflammatory effects, and consequently kidney and cardiovascular diseases. Low clearance rate of these uremic toxins from the blood of uremic patients by conventional techniques like hemodialysis is due to their strong covalent albumin binding (greater than 95%) and hydrophobic nature, which led to alternatives like usage of hydrophobic adsorber&amp;amp;#39;s in removing these toxins from the plasma of kidney patients. Polymers like polyethylene, polyurethane, polymethylmethacrylate, cellophane and polytetrafluoroethylene were already in use as substitutes for metal devices as dialysis membranes. Among new synthetic polymers, one such ideal adsorber material are highly porous microparticles of poly(ether imide) (PEI) with diameters in the range from 50-180μm and a porosity around 88±2% prepared by a spraying and coagulation process.It is essential to make sure that these synthetic polymers should not evoke any inflammatory or apoptotic response during dialysis. Therefore in our study we evaluated in vitro effect of PEI microparticle extracts in human aortic endothelial cells (HEACs) concerning toxicity, inflammation and apoptosis. No cell toxicity was observed when HAECs were treated with PEI extracts and inflammatory/apoptotic markers were not upregulated in presence of PEI extracts. Our results ensure biocompatibility of PEI particles and further hemocompatibility of particles will be tested.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Adsorption capacity of poly(ether imide) microparticles to uremic toxins

Clinical Hemorheology and Microcirculation, 2016

Uremia is a phenomenon caused by retention of uremic toxins in the plasma due to functional impai... more Uremia is a phenomenon caused by retention of uremic toxins in the plasma due to functional impairment of kidneys in the elimination of urinary waste products. Uremia is presently treated by dialysis techniques like hemofiltration, dialysis or hemodiafiltration. However, these techniques in use are more favorable towards removing hydrophilic than hydrophobic uremic toxins. Hydrophobic uremic toxins, such as hydroxy hipuric acid (OH-HPA), phenylacetic acid (PAA), indoxyl sulfate (IDS) and p-cresylsulfate (pCRS), contribute substantially to the progression of chronic kidney disease (CKD) and cardiovascular disease. Therefore, objective of the present study is to test adsorption capacity of highly porous microparticles prepared from poly(ether imide) (PEI) as an alternative technique for the removal of uremic toxins. Two types of nanoporous, spherically shaped microparticles were prepared from PEI by a spraying/coagulation process.PEI particles were packed into a preparative HPLC column to which a mixture of the four types of uremic toxins was injected and eluted with ethanol. Eluted toxins were quantified by analytical HPLC. PEI particles were able to adsorb all four toxins, with the highest affinity for PAA and pCR. IDS and OH-HPA showed a partially non-reversible binding. In summary, PEI particles are interesting candidates to be explored for future application in CKD.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of extracts of poly(ether imide) microparticles on cytotoxicity, ROS generation and proinflammatory effects on human monocytic (THP-1) cells

Clinical hemorheology and microcirculation, Jan 26, 2015

Current haemodialysis techniques are not capable to remove efficiently low molecular weight hydro... more Current haemodialysis techniques are not capable to remove efficiently low molecular weight hydrophobic uremic toxins from the blood of patients suffering from chronic renal failure. With respect to the hydrophobic characteristics and the high level of protein binding of these uremic toxins, hydrophobic adsorber materials might be an alternative to remove these substances from the plasma of the chronic kidney disease (CKD) patients. Here nanoporous microparticles prepared from poly(ether imide) (PEI) with an average diameter of 90±30μm and a porosity around 88±2% prepared by a spraying/coagulation process are considered as candidate adsorber materials. A prerequisite for the clinical application of such particles is their biocompatibility, which can be examined i.e. indirectly in cell culture experiments with the particles' extracts. In this work we studied the effects of aqueous extracts of PEI microparticles on the viability of THP-1 cells, a human leukemia monocytic cell line...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size

Planta, 2003

DNA insertional mutagenesis and screening of the green alga Chlamydomonas reinhardtii was employe... more DNA insertional mutagenesis and screening of the green alga Chlamydomonas reinhardtii was employed to isolate tla1, a stable transformant having a truncated light-harvesting chlorophyll antenna size. Molecular analysis showed a single plasmid insertion into an open reading frame of the nuclear genome corresponding to a novel gene ( Tla1) that encodes a protein of 213 amino acids. Genetic analysis showed co-segregation of plasmid and tla1 phenotype. Biochemical analyses showed the tla1 mutant to be chlorophyll deficient, with a functional chlorophyll antenna size of photosystem I and photosystem II being about 50% and 65% of that of the wild type, respectively. It contained a correspondingly lower amount of light-harvesting proteins than the wild type and had lower steady-state levels of Lhcb mRNA. The tla1 strain required a higher light intensity for the saturation of photosynthesis and showed greater solar conversion efficiencies and a higher photosynthetic productivity than the wi...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Testosterone-derived estradiol production by male endothelium is robust and dependent on p450 aromatase via estrogen receptor alpha

SpringerPlus, 2013

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

THE PLANT CELL ONLINE, 2004

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Postprandial apoE Isoform and Conformational Changes Associated with VLDL Lipolysis Products Modulate Monocyte Inflammation

PLoS ONE, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii is regulated by the novel Tla1 gene

Planta, 2007

Bookmarks Related papers MentionsView impact

Research paper thumbnail of VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation

The Journal of Lipid Research, 2010

Bookmarks Related papers MentionsView impact

Research paper thumbnail of C-terminal interactions of apolipoprotein E4 respond to the postprandial state

The Journal of Lipid Research, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Gum resin of Boswellia serrata inhibited human monocytic (THP-1) cell activation and platelet aggregation

Journal of Ethnopharmacology, 2011

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Stem-bark of Terminalia arjuna attenuates human monocytic (THP-1) and aortic endothelial cell activation

Journal of Ethnopharmacology, 2013

Bookmarks Related papers MentionsView impact