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Research paper thumbnail of Iron responsive protein-2 is associated with lung function and disease progression in COPD

Research paper thumbnail of Effect of WISP-1 on Collagen Turnover in Primary Airway Smooth Muscle Cells: Implications for Asthma and Copd Airway Remodeling

Research paper thumbnail of Association between formylglycine-generating enzyme variants and lung function in patients with COPD

Research paper thumbnail of Activation of the IGF-1 pathway by Trofinetide as a novel therapeutic approach for asthma and COPD

Research paper thumbnail of Sulfatase modifying factor-1 common polymorphisms are associated with response to inhaled corticosteroids in COPD

Research paper thumbnail of Collagen turnover in airway smooth muscle cells is mediated by WISP-1 differently in asthma and in COPD

Airway cell biology and immunopathology

Research paper thumbnail of Genetic variants in the Iron responsive element binding protein 2 (IREB2) are associated with spirometry parameters and cough prevalence

Molecular pathology and functional genomics

Research paper thumbnail of Clinical, Histological, and Molecular Prognostic Factors in Childhood Medulloblastoma: Where Do We Stand?

Diagnostics, May 30, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Polymorphisms in the Sulfatase Modifying Factor 1 (SUMF1) gene are associated with lung function parameters in patients with COPD

Molecular pathology and functional genomics

Research paper thumbnail of Retinal Vascular Lesions in Patients with Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis

Journal of Personalized Medicine

Background: This systematic review and meta-analysis aimed to summarize and compare data on retin... more Background: This systematic review and meta-analysis aimed to summarize and compare data on retinal vascular lesions between patients with nonalcoholic fatty liver disease (NAFLD) and individuals without the disease. Methods: Search was performed in PubMed, Scopus and Cochrane Library, complemented by handsearching (PROSPERO ID: CRD42022345558). Thirty-six studies comprising 24,985 individuals (12,387 NAFLD patients and 12,598 controls) were selected for the meta-analysis. Results: Apart from retinopathy, no study with a different type of retinal vascular lesion was retrieved. Overall, there was no significant difference in the presence of retinopathy in NAFLD patients compared to controls (Odds Ratio (OR) = 1.20; 95% Confidence Interval (CI): 0.91–1.59). Heterogeneity among studies was high (I2 = 93%; p < 0.00001), while Egger’s test revealed no publication bias (p = 0.60). However, subgroup analysis showed positive association between retinopathy and NAFLD in type 1 diabetes me...

Research paper thumbnail of An association study of the PNPLA3 I148M polymorphism (rs738409) with serum lipids in patients with dyslipidemia

Exploration of Medicine

Aim: One single nucleotide polymorphism (SNP) rs738409 in the patatin-like phospholipase domain-c... more Aim: One single nucleotide polymorphism (SNP) rs738409 in the patatin-like phospholipase domain-containing protein 3 (PNPLA3) gene has been considered a major genetic risk factor of nonalcoholic fatty liver disease (NAFLD). Data have indicated that NAFLD is related to insulin resistance and dyslipidemia, but whether rs738409 is associated with circulating lipid and lipoproteins is not fully elucidated. The main aim of this study was to assess the association of rs738409 with lipid and lipoprotein levels in patients with dyslipidemia. Methods: This was a post-hoc analysis of a study in patients with dyslipidemia recruited on an outpatient basis. Morning blood samples were collected after a 12-h fast. Genomic DNA was extracted from whole-blood samples. Results: One hundred seventy-five patients with dyslipidemia were included (97 women). Lipid levels [total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C)]...

Research paper thumbnail of Effect of common OPRM1, COMT, SLC6A4, ABCB1, and CYP2B6 polymorphisms on perioperative analgesic and propofol demands on patients subjected to thyroidectomy surgery

Pharmacological Reports

Background Perioperative anesthetic and/or analgesic demand present considerable variation, and p... more Background Perioperative anesthetic and/or analgesic demand present considerable variation, and part of that variation appears to be genetic in origin. Here we investigate the impact of common polymorphisms in OPRM1, COMT, SLC6A4, ABCB1, and CYP2B6 genes, on the intra-operative consumption of remifentanil and propofol, as well as the postoperative analgesic needs, in patients subjected to thyroidectomy surgery. Methods We conducted a prospective cohort study with 90 patients scheduled to undergo elective thyroidectomy, under total intravenous anesthesia achieved by target control infusion (TCI) of propofol and remifentanil. Postoperative analgesics were administered by protocol and on-demand by the individual patient. Genotyping was established by PCR–RFLP methods. Genotyping data, intra-operative hemodynamics, and total consumption of remifentanil and propofol, as well as postoperative analgesic needs and pain perception, were recorded for each individual. Results Patients with the...

Research paper thumbnail of An Innovative PTD-IVT-mRNA Delivery Platform for CAR Immunotherapy of ErbB(+) Solid Tumor Neoplastic Cells

Biomedicines

Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells t... more Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells to carry such a receptor and, thus, recognize cancer cell surface antigens. Viral transfection is currently the preferred method, but it carries the risk of off-target mutagenicity. Other transfection platforms have thus been proposed, such the in vitro transcribed (IVT)-mRNAs. In this study, we exploited our innovative, patented delivery platform to produce protein transduction domain (PTD)-IVT-mRNAs for the expression of CAR on NK-92 cells. CAR T1E-engineered NK-92 cells, harboring the sequence of T1E single-chain fragment variant (scFv) to recognize the ErbB receptor, bearing either CD28 or 4-1BB as co-stimulatory signaling domains, were prepared and assessed for their effectiveness in two different ErbB(+) cancer cell lines. Our results showed that the PTD-IVT-mRNA of CAR was safely transduced and expressed into NK-92 cells. CAR T1E-engineered NK-92 cells provoked high levels of cell ...

Research paper thumbnail of Rupatadine effectively prevents the histamine-induced up regulation of histamine H1R and bradykinin B2R receptor gene expression in the rat paw

Pharmacological reports : PR, 2014

Activation of histamine H1 receptor (H1R) is a well-known hallmark of allergic and inflammatory p... more Activation of histamine H1 receptor (H1R) is a well-known hallmark of allergic and inflammatory pathology. Both types of bradykinin receptors (B1R and B2R) are also known to contribute significantly to the latter and some sort of functional interaction between them and H1R has been alluded to in the past. Here we use an experimental model of rat paw oedema formation to examine the effect of exogenously added histamine on the gene expression of H1R and bradykinin receptors B1R and B2R, alone or in combination to rupatadine, a second generation antihistamine agent. Histamine-induced oedema formation was monitored with a plethysmometer. The gene expression of H1R, B1R and B2R was analyzed with both conventional and real-time PCR. Rupatadine fumarate was used in pure form and administered intraperitoneally, prior to histamine injection into the paw. Microscopy of haematoxylin and eosin-stained sections of paw tissue was used to examine effects on tissue architecture. Histamine injection...

Research paper thumbnail of The effect of pharmacological cessation and restoration of menstrual cycle on bone metabolism in premenopausal women with endometriosis

Research paper thumbnail of A Study of Blood Fatty Acids Profile in Hyperlipidemic and Normolipidemic Subjects in Association with Common PNPLA3 and ABCB1 Polymorphisms

Metabolites, 2021

Adiponutrin (patatin-like phospholipase domain-containing 3; PNPLA3), encoded in humans by the PN... more Adiponutrin (patatin-like phospholipase domain-containing 3; PNPLA3), encoded in humans by the PNPLA3 gene, is a protein associated with lipid droplet and endoplasmic reticulum membranes, where it is apparently involved in fatty acid redistribution between triglycerides and phospholipids. A common polymorphism of PNPLA3 (I148M, rs738409), linked to increased PNPLA3 presence on lipid droplets, is a strong genetic determinant of non-alcoholic fatty liver disease (NAFLD) and of its progression. P-glycoprotein (Pgp, MDR1—multidrug resistance protein 1, ABCB1—ATP-binding cassette sub-family B member 1), encoded by the ABCB1 gene, is another membrane protein implicated in lipid homeostasis and steatosis. In the past, common ABCB1 polymorphisms have been associated with the distribution of serum lipids but not with fatty acids (FA) profiles. Similarly, data on the effect of PNPLA3 I148M polymorphism on blood FAs are scarce. In this study, a gas chromatography-flame ionization detection (GC...

Research paper thumbnail of Circulating sclerostin levels during denosumab discontinuation and the subsequent early or late zoledronate infusion

Research paper thumbnail of Ιrisin Levels in Postmenopausal Women with an Incident Hip Fracture

Irisin, a myokine mainly produced by the skeletal muscle [1], has been suggested as a major regul... more Irisin, a myokine mainly produced by the skeletal muscle [1], has been suggested as a major regulator of the cross-talk between muscle and bone [2, 3]. In human studies, circulating irisin has been positively associated with bone status in healthy children [4], young athletes [5], and older patients undergoing hip or knee replacement [6], and more importantly, inversely associated with prevalent osteoporotic fractures in postmenopausal women [7–9], independently from other factors associated with bone fragility, including BMD and bone turnover markers [7]. In the present study we evaluated circulating irisin levels at the time of fracture in postmenopausal women who sustained a hip fracture compared to controls, being postmenopausal women with osteoarthritis before undergoing hip or knee arthroplasty. Methods

Research paper thumbnail of Antibody Titers 3-Months Post-Vaccination with the Pfizer/Biontech Vaccine in Greece

Research paper thumbnail of Comparative Effect of Zoledronate at 6 Versus 18 Months Following Denosumab Discontinuation

Calcified Tissue International

Research paper thumbnail of Iron responsive protein-2 is associated with lung function and disease progression in COPD

Research paper thumbnail of Effect of WISP-1 on Collagen Turnover in Primary Airway Smooth Muscle Cells: Implications for Asthma and Copd Airway Remodeling

Research paper thumbnail of Association between formylglycine-generating enzyme variants and lung function in patients with COPD

Research paper thumbnail of Activation of the IGF-1 pathway by Trofinetide as a novel therapeutic approach for asthma and COPD

Research paper thumbnail of Sulfatase modifying factor-1 common polymorphisms are associated with response to inhaled corticosteroids in COPD

Research paper thumbnail of Collagen turnover in airway smooth muscle cells is mediated by WISP-1 differently in asthma and in COPD

Airway cell biology and immunopathology

Research paper thumbnail of Genetic variants in the Iron responsive element binding protein 2 (IREB2) are associated with spirometry parameters and cough prevalence

Molecular pathology and functional genomics

Research paper thumbnail of Clinical, Histological, and Molecular Prognostic Factors in Childhood Medulloblastoma: Where Do We Stand?

Diagnostics, May 30, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Polymorphisms in the Sulfatase Modifying Factor 1 (SUMF1) gene are associated with lung function parameters in patients with COPD

Molecular pathology and functional genomics

Research paper thumbnail of Retinal Vascular Lesions in Patients with Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis

Journal of Personalized Medicine

Background: This systematic review and meta-analysis aimed to summarize and compare data on retin... more Background: This systematic review and meta-analysis aimed to summarize and compare data on retinal vascular lesions between patients with nonalcoholic fatty liver disease (NAFLD) and individuals without the disease. Methods: Search was performed in PubMed, Scopus and Cochrane Library, complemented by handsearching (PROSPERO ID: CRD42022345558). Thirty-six studies comprising 24,985 individuals (12,387 NAFLD patients and 12,598 controls) were selected for the meta-analysis. Results: Apart from retinopathy, no study with a different type of retinal vascular lesion was retrieved. Overall, there was no significant difference in the presence of retinopathy in NAFLD patients compared to controls (Odds Ratio (OR) = 1.20; 95% Confidence Interval (CI): 0.91–1.59). Heterogeneity among studies was high (I2 = 93%; p < 0.00001), while Egger’s test revealed no publication bias (p = 0.60). However, subgroup analysis showed positive association between retinopathy and NAFLD in type 1 diabetes me...

Research paper thumbnail of An association study of the PNPLA3 I148M polymorphism (rs738409) with serum lipids in patients with dyslipidemia

Exploration of Medicine

Aim: One single nucleotide polymorphism (SNP) rs738409 in the patatin-like phospholipase domain-c... more Aim: One single nucleotide polymorphism (SNP) rs738409 in the patatin-like phospholipase domain-containing protein 3 (PNPLA3) gene has been considered a major genetic risk factor of nonalcoholic fatty liver disease (NAFLD). Data have indicated that NAFLD is related to insulin resistance and dyslipidemia, but whether rs738409 is associated with circulating lipid and lipoproteins is not fully elucidated. The main aim of this study was to assess the association of rs738409 with lipid and lipoprotein levels in patients with dyslipidemia. Methods: This was a post-hoc analysis of a study in patients with dyslipidemia recruited on an outpatient basis. Morning blood samples were collected after a 12-h fast. Genomic DNA was extracted from whole-blood samples. Results: One hundred seventy-five patients with dyslipidemia were included (97 women). Lipid levels [total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C)]...

Research paper thumbnail of Effect of common OPRM1, COMT, SLC6A4, ABCB1, and CYP2B6 polymorphisms on perioperative analgesic and propofol demands on patients subjected to thyroidectomy surgery

Pharmacological Reports

Background Perioperative anesthetic and/or analgesic demand present considerable variation, and p... more Background Perioperative anesthetic and/or analgesic demand present considerable variation, and part of that variation appears to be genetic in origin. Here we investigate the impact of common polymorphisms in OPRM1, COMT, SLC6A4, ABCB1, and CYP2B6 genes, on the intra-operative consumption of remifentanil and propofol, as well as the postoperative analgesic needs, in patients subjected to thyroidectomy surgery. Methods We conducted a prospective cohort study with 90 patients scheduled to undergo elective thyroidectomy, under total intravenous anesthesia achieved by target control infusion (TCI) of propofol and remifentanil. Postoperative analgesics were administered by protocol and on-demand by the individual patient. Genotyping was established by PCR–RFLP methods. Genotyping data, intra-operative hemodynamics, and total consumption of remifentanil and propofol, as well as postoperative analgesic needs and pain perception, were recorded for each individual. Results Patients with the...

Research paper thumbnail of An Innovative PTD-IVT-mRNA Delivery Platform for CAR Immunotherapy of ErbB(+) Solid Tumor Neoplastic Cells

Biomedicines

Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells t... more Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells to carry such a receptor and, thus, recognize cancer cell surface antigens. Viral transfection is currently the preferred method, but it carries the risk of off-target mutagenicity. Other transfection platforms have thus been proposed, such the in vitro transcribed (IVT)-mRNAs. In this study, we exploited our innovative, patented delivery platform to produce protein transduction domain (PTD)-IVT-mRNAs for the expression of CAR on NK-92 cells. CAR T1E-engineered NK-92 cells, harboring the sequence of T1E single-chain fragment variant (scFv) to recognize the ErbB receptor, bearing either CD28 or 4-1BB as co-stimulatory signaling domains, were prepared and assessed for their effectiveness in two different ErbB(+) cancer cell lines. Our results showed that the PTD-IVT-mRNA of CAR was safely transduced and expressed into NK-92 cells. CAR T1E-engineered NK-92 cells provoked high levels of cell ...

Research paper thumbnail of Rupatadine effectively prevents the histamine-induced up regulation of histamine H1R and bradykinin B2R receptor gene expression in the rat paw

Pharmacological reports : PR, 2014

Activation of histamine H1 receptor (H1R) is a well-known hallmark of allergic and inflammatory p... more Activation of histamine H1 receptor (H1R) is a well-known hallmark of allergic and inflammatory pathology. Both types of bradykinin receptors (B1R and B2R) are also known to contribute significantly to the latter and some sort of functional interaction between them and H1R has been alluded to in the past. Here we use an experimental model of rat paw oedema formation to examine the effect of exogenously added histamine on the gene expression of H1R and bradykinin receptors B1R and B2R, alone or in combination to rupatadine, a second generation antihistamine agent. Histamine-induced oedema formation was monitored with a plethysmometer. The gene expression of H1R, B1R and B2R was analyzed with both conventional and real-time PCR. Rupatadine fumarate was used in pure form and administered intraperitoneally, prior to histamine injection into the paw. Microscopy of haematoxylin and eosin-stained sections of paw tissue was used to examine effects on tissue architecture. Histamine injection...

Research paper thumbnail of The effect of pharmacological cessation and restoration of menstrual cycle on bone metabolism in premenopausal women with endometriosis

Research paper thumbnail of A Study of Blood Fatty Acids Profile in Hyperlipidemic and Normolipidemic Subjects in Association with Common PNPLA3 and ABCB1 Polymorphisms

Metabolites, 2021

Adiponutrin (patatin-like phospholipase domain-containing 3; PNPLA3), encoded in humans by the PN... more Adiponutrin (patatin-like phospholipase domain-containing 3; PNPLA3), encoded in humans by the PNPLA3 gene, is a protein associated with lipid droplet and endoplasmic reticulum membranes, where it is apparently involved in fatty acid redistribution between triglycerides and phospholipids. A common polymorphism of PNPLA3 (I148M, rs738409), linked to increased PNPLA3 presence on lipid droplets, is a strong genetic determinant of non-alcoholic fatty liver disease (NAFLD) and of its progression. P-glycoprotein (Pgp, MDR1—multidrug resistance protein 1, ABCB1—ATP-binding cassette sub-family B member 1), encoded by the ABCB1 gene, is another membrane protein implicated in lipid homeostasis and steatosis. In the past, common ABCB1 polymorphisms have been associated with the distribution of serum lipids but not with fatty acids (FA) profiles. Similarly, data on the effect of PNPLA3 I148M polymorphism on blood FAs are scarce. In this study, a gas chromatography-flame ionization detection (GC...

Research paper thumbnail of Circulating sclerostin levels during denosumab discontinuation and the subsequent early or late zoledronate infusion

Research paper thumbnail of Ιrisin Levels in Postmenopausal Women with an Incident Hip Fracture

Irisin, a myokine mainly produced by the skeletal muscle [1], has been suggested as a major regul... more Irisin, a myokine mainly produced by the skeletal muscle [1], has been suggested as a major regulator of the cross-talk between muscle and bone [2, 3]. In human studies, circulating irisin has been positively associated with bone status in healthy children [4], young athletes [5], and older patients undergoing hip or knee replacement [6], and more importantly, inversely associated with prevalent osteoporotic fractures in postmenopausal women [7–9], independently from other factors associated with bone fragility, including BMD and bone turnover markers [7]. In the present study we evaluated circulating irisin levels at the time of fracture in postmenopausal women who sustained a hip fracture compared to controls, being postmenopausal women with osteoarthritis before undergoing hip or knee arthroplasty. Methods

Research paper thumbnail of Antibody Titers 3-Months Post-Vaccination with the Pfizer/Biontech Vaccine in Greece

Research paper thumbnail of Comparative Effect of Zoledronate at 6 Versus 18 Months Following Denosumab Discontinuation

Calcified Tissue International