Huma Akbar | University of Sargodha (original) (raw)

Papers by Huma Akbar

Research paper thumbnail of The Study of Histopathological Changes in the Gonads of Frog upon Exposure to Lambda Cyhalothrin

International Journal of Scientific and Engineering Research

Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they ca... more Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they cause noteworthy risk to non ‐targ eted organisms. This present work includes susceptible species such as amphibians show venomous effect and death by extensive used pesticide lambda cyhalothrin in rice fields of Pakistan and this ecological alteration may initiate a reduced biodiversity, trophic cascades' damage and ultimate harm to public health. Analysis has been made to assess the influence of various concentrations of lambda cyhalothrin on several histopathological constraints of gonads of frog. In this work 40 frogs were divided into four groups A (control), B(low lethal), C(median lethal) and D(high lethal) at no, 0.008,0.010 and 0.012mg/litre doses of different weight retaining frogs, respectively. The doses were given in water of aquarium mg/litre at 20th June,2014 and frogs in took them through cutaneous respiration and inhalation. Effect differs with frogs' weight and mo...

Research paper thumbnail of Nutritional analysis of Rhazya stricta collected from different sites of Kalabagh, Mianwali, Pakistan

Pure and Applied Biology, 2020

Research paper thumbnail of BLOOD GROUP AND Β-THALASSEMIA MAJOR  IN DISTRICT SWAT, KPK, PAKISTAN

Thalassemia are defined as a group of inherited hematological disorders characterized by early on... more Thalassemia are defined as a group of inherited hematological disorders characterized by early onset of anemia resulting from
reduced rate of synthesis of one or more globin chains caused by globin chain mutations. The present study was designed to find out the
occurrence and comparison (prevalence) of beta thalassemia major in the population of Urban (Mingora) and Rural (tehsil Kabal) localities
of District Swat. Occurrence of thalassemic patients in both the localities were categorized, regarding three aspects i.e. age wise, gender
wise and blood group wise. It was also examined to test out if there is any correlation of blood group with the disease or not? Prevalence
ratio of both the localities was also determined. Data of patients was collected from Wajeeha thalassemia Center Swat and Alfajr Foundation
Swat. In the total population of 440000 persons of Mingora, 35 (0.008%), while in that of 170000 population of tehsil Kabal 42 (0.024%)
registered patients were reported. Out of all these 70 patients, 40 (51.94%) were male while 37 (48.05%) were female. 24 (31.16%) were in
age group 1-5, 25 (32.46%) in age group 6-10, 17 (22.7%) in 11-15, 08 (10.38%) in 16-20 and 03 (03.89%) were in age group 21-25. There
were no patients above the age of 25. In blood group wise distribution, as Rh positive blood group occur 85% in nature, so the individuals
having these group in our data were examined to be 74 out of 77 in which A+ were 22 (31.42%), no one in A-, 26 were B+ (33.76%), one
was B- (01.29%), 3 were AB+ (03.89%), no one in AB-, 23 were O+ (29.87%) and 2 were O- (02.59%). This shows that there is no correlation
of the disease with blood group. The recent study will provide reports and recommendations to the concerned field and will make awareness
of beta thalassemia major disease among patients and public for their safety. Moreover, same study should be conducted in large population
size to determine the other risk factors.
Index Terms— Thalassemias , Alpha-thalassemia , β thalassaemia , iron-deficiency anemia, Anemiais.

Research paper thumbnail of PREVALENCE OF HCV IN TEHSIL BABOZI DISTRICT SWAT, Khyber -Pakhtunkhwa, Pakistan

Hepatitis C is a major health problem caused by the HCV which is predomint and associated with li... more Hepatitis C is a major health problem caused by the HCV which is predomint and associated with liver cirrhosis and hepatocellular carcinoma. About 170 million people are infected with chronic Hepatitis C. There is no current vaccine existing while the vitamin D shortage is a common reason for treatment failure in a person with Hepatitis C virus. The study was conducted in Tehsil Babozai District Swat to investigate the prevalence of Hepatitis C. Data was collected from 9 Union councils in the area comprising of 223 individuals of age 15 to 65. After collection samples were tested in the laboratory to find out the percentage of positive cases. Ratio of Hepatitis C positive cases in males was 1.78% and 5.66% in females, which shows a remarkable higher incidence of infection in females. Ratio of Hepatitis C positive cases was 1.6 % in the age group 15-26, 4% in age group 46_55, and 6.25% in age group 56_65 which indicate the high vulnerability of old age group (56-65 years) to Hepatitis C infection. The occupation wise positive cases ratio was 6.38% in house wives, 5.5% of farmers, 2.63% in shopkeepers and 3.33% of students while respondents belonging to other professions were found negative for Hepatitis C infection. This ratio shows majority of Hepatitis C positive cases in housewives and farmers living in peripheral localities in the study area, where there is lack of education and health facilities. The main reason of the occurrence of Hepatitis C infection was found to be lack of proper blood screening, sterilized surgical equipment's and news razors in barber shop as well as reuse of an intravenous syringe in local health care centers and hospital. To combat Hepatitis C infection in this area there is a need of awareness complains, proper education and check and balance in health care units in hospital of Swat.

Research paper thumbnail of Nutritional analysis of Rhazya stricta collected from different sites of Kalabagh, Mianwali, Pakistan

Pure Appl. Biol, 2020

Medicinal plants have constantly played significant parts in fields of culture, medicine and nutr... more Medicinal plants have constantly played significant parts in fields of culture, medicine and nutrition. Rhazya stricta is a small, upright perennial, poisonous shrub. Different plant parts have been used in medicine traditionally in contradiction of many diseases like diabetes, skin diseases, foot burning, stomach pain. The aim of research work was to study different nutrient composition in Rhazya stricta collected from different sites of Kalabagh, Mianwali. By using AOAC standard methods moisture content, fat content, fiber and protein content were checked. Proximate analysis showed plant besides having medicinal importance plant have moderate nutritional composition. There is little variation in nutrients of Rhazya stricta collected from different sites because of little variation in quality of soil and climatic conditions. It is concluded that medicinal plants should also be studied for their nutritional composition beside medicinal importance.

Research paper thumbnail of Role of anuran tadpoles as biological control for dengue larvae

International Journal of Mosquito Research, 2019

Dengue is a viral disease caused by Aedes mosquitoes (Diptera: Culicidae) and cause serious illne... more Dengue is a viral disease caused by Aedes mosquitoes (Diptera: Culicidae) and cause serious illness in people community living in tropics. It is able to cause bundles of symptoms and may cause bleeding internally leading to death of the patient. Currently there is no immunization against dengue disorder but the most broadly utilized techniques for control of mosquitoes are usage of insecticides and biological control. Present study has been designed to observe and compare the consumption of dengue mosquito larvae by two anuran species, Common Skittering Frog (Euphlyctis cyanophlyctis) and Southeast Asian Toad (Duttaphrynus melanostictus) tadpoles inhabiting Rawalpindi and Islamabad. The tadpoles and dengue larvae were collected from ponds and pools using dip nets. Six trails were carried with anuran species of common skittering frog and Southeast Asian toad. Each trail was replicated thrice. The Kruskal-Wallis test present that the total number of consumed dengue larvae among various trials between two species (Common Skittering Frog and Southeast Asian Toad) did not differ-significantly (P=0.26) and (P=0.08), while the Wilcoxon test showed that the number of consumed dengue larvae by tadpoles of the two anuran species was P=0.08 which also did not differ significantly. Therefore it was concluded that the Southeast Asian toad is better in the consumption of dengue mosquito larvae as compared to common skittering frog.

Research paper thumbnail of The Study of Histopathological Changes in the Gonads of Frog

International Journal of Scientific & Engineering Research, Volume 5, 2014

ABSTRACT-Pesticides frequently premeditated a quick source for scheming weeds and insect pests bu... more ABSTRACT-Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they cause noteworthy risk to non ‐targeted organisms. This present work includes susceptible species such as amphibians show venomous effect and death by extensive used pesticide lambda cyhalothrin in rice fields of Pakistan and this ecological alteration may initiate a reduced biodiversity, trophic cascades’ damage and ultimate harm to public health. Analysis has been made to assess the influence of various concentrations of lambda cyhalothrin on several histopathological constraints of gonads of frog. In this work 40 frogs were divided into four groups A (control), B(low lethal), C(median lethal) and D(high lethal) at no, 0.008,0.010 and 0.012mg/litre doses of different weight retaining frogs, respectively. The doses were given in water of aquarium mg/litre at 20th June,2014 and frogs in took them through cutaneous respiration and inhalation. Effect differs with frogs’ weight and morphological and histological alterations were observed in control group compared to treated group. At 2nd and 3rd day, frogs were pithed and observations concerning to morphology and histology of gonads were documented in all treatments, which incorporated discoloration and disruption of gonads and total degeneration of testis in less weighty frogs in morphological changes. Histopathological changes were foremost and included breaking of cyst walls, bunching of spermatocytes, necrosis of sertoli cells and sprinkled masses of decayed sperm bundles etc in testis and contraction in follicles, reduction in the number of oocytes, edema in stroma and formation of denatured yolk and necrosis etc. in ovaries which concluded that it has adverse affect on fertility of amphibians ultimately reducing their population size at all doses.
INDEX TERMS: Amphibian, Lambda-Cyhalothrin, Frog, Gonads, Rice fields, Decline, Pakistan

Research paper thumbnail of The Study of Histopathological Changes in the Gonads of Frog upon Exposure to Lambda Cyhalothrin

International Journal of Scientific and Engineering Research

Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they ca... more Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they cause noteworthy risk to non ‐targ eted organisms. This present work includes susceptible species such as amphibians show venomous effect and death by extensive used pesticide lambda cyhalothrin in rice fields of Pakistan and this ecological alteration may initiate a reduced biodiversity, trophic cascades' damage and ultimate harm to public health. Analysis has been made to assess the influence of various concentrations of lambda cyhalothrin on several histopathological constraints of gonads of frog. In this work 40 frogs were divided into four groups A (control), B(low lethal), C(median lethal) and D(high lethal) at no, 0.008,0.010 and 0.012mg/litre doses of different weight retaining frogs, respectively. The doses were given in water of aquarium mg/litre at 20th June,2014 and frogs in took them through cutaneous respiration and inhalation. Effect differs with frogs' weight and mo...

Research paper thumbnail of Nutritional analysis of Rhazya stricta collected from different sites of Kalabagh, Mianwali, Pakistan

Pure and Applied Biology, 2020

Research paper thumbnail of BLOOD GROUP AND Β-THALASSEMIA MAJOR  IN DISTRICT SWAT, KPK, PAKISTAN

Thalassemia are defined as a group of inherited hematological disorders characterized by early on... more Thalassemia are defined as a group of inherited hematological disorders characterized by early onset of anemia resulting from
reduced rate of synthesis of one or more globin chains caused by globin chain mutations. The present study was designed to find out the
occurrence and comparison (prevalence) of beta thalassemia major in the population of Urban (Mingora) and Rural (tehsil Kabal) localities
of District Swat. Occurrence of thalassemic patients in both the localities were categorized, regarding three aspects i.e. age wise, gender
wise and blood group wise. It was also examined to test out if there is any correlation of blood group with the disease or not? Prevalence
ratio of both the localities was also determined. Data of patients was collected from Wajeeha thalassemia Center Swat and Alfajr Foundation
Swat. In the total population of 440000 persons of Mingora, 35 (0.008%), while in that of 170000 population of tehsil Kabal 42 (0.024%)
registered patients were reported. Out of all these 70 patients, 40 (51.94%) were male while 37 (48.05%) were female. 24 (31.16%) were in
age group 1-5, 25 (32.46%) in age group 6-10, 17 (22.7%) in 11-15, 08 (10.38%) in 16-20 and 03 (03.89%) were in age group 21-25. There
were no patients above the age of 25. In blood group wise distribution, as Rh positive blood group occur 85% in nature, so the individuals
having these group in our data were examined to be 74 out of 77 in which A+ were 22 (31.42%), no one in A-, 26 were B+ (33.76%), one
was B- (01.29%), 3 were AB+ (03.89%), no one in AB-, 23 were O+ (29.87%) and 2 were O- (02.59%). This shows that there is no correlation
of the disease with blood group. The recent study will provide reports and recommendations to the concerned field and will make awareness
of beta thalassemia major disease among patients and public for their safety. Moreover, same study should be conducted in large population
size to determine the other risk factors.
Index Terms— Thalassemias , Alpha-thalassemia , β thalassaemia , iron-deficiency anemia, Anemiais.

Research paper thumbnail of PREVALENCE OF HCV IN TEHSIL BABOZI DISTRICT SWAT, Khyber -Pakhtunkhwa, Pakistan

Hepatitis C is a major health problem caused by the HCV which is predomint and associated with li... more Hepatitis C is a major health problem caused by the HCV which is predomint and associated with liver cirrhosis and hepatocellular carcinoma. About 170 million people are infected with chronic Hepatitis C. There is no current vaccine existing while the vitamin D shortage is a common reason for treatment failure in a person with Hepatitis C virus. The study was conducted in Tehsil Babozai District Swat to investigate the prevalence of Hepatitis C. Data was collected from 9 Union councils in the area comprising of 223 individuals of age 15 to 65. After collection samples were tested in the laboratory to find out the percentage of positive cases. Ratio of Hepatitis C positive cases in males was 1.78% and 5.66% in females, which shows a remarkable higher incidence of infection in females. Ratio of Hepatitis C positive cases was 1.6 % in the age group 15-26, 4% in age group 46_55, and 6.25% in age group 56_65 which indicate the high vulnerability of old age group (56-65 years) to Hepatitis C infection. The occupation wise positive cases ratio was 6.38% in house wives, 5.5% of farmers, 2.63% in shopkeepers and 3.33% of students while respondents belonging to other professions were found negative for Hepatitis C infection. This ratio shows majority of Hepatitis C positive cases in housewives and farmers living in peripheral localities in the study area, where there is lack of education and health facilities. The main reason of the occurrence of Hepatitis C infection was found to be lack of proper blood screening, sterilized surgical equipment's and news razors in barber shop as well as reuse of an intravenous syringe in local health care centers and hospital. To combat Hepatitis C infection in this area there is a need of awareness complains, proper education and check and balance in health care units in hospital of Swat.

Research paper thumbnail of Nutritional analysis of Rhazya stricta collected from different sites of Kalabagh, Mianwali, Pakistan

Pure Appl. Biol, 2020

Medicinal plants have constantly played significant parts in fields of culture, medicine and nutr... more Medicinal plants have constantly played significant parts in fields of culture, medicine and nutrition. Rhazya stricta is a small, upright perennial, poisonous shrub. Different plant parts have been used in medicine traditionally in contradiction of many diseases like diabetes, skin diseases, foot burning, stomach pain. The aim of research work was to study different nutrient composition in Rhazya stricta collected from different sites of Kalabagh, Mianwali. By using AOAC standard methods moisture content, fat content, fiber and protein content were checked. Proximate analysis showed plant besides having medicinal importance plant have moderate nutritional composition. There is little variation in nutrients of Rhazya stricta collected from different sites because of little variation in quality of soil and climatic conditions. It is concluded that medicinal plants should also be studied for their nutritional composition beside medicinal importance.

Research paper thumbnail of Role of anuran tadpoles as biological control for dengue larvae

International Journal of Mosquito Research, 2019

Dengue is a viral disease caused by Aedes mosquitoes (Diptera: Culicidae) and cause serious illne... more Dengue is a viral disease caused by Aedes mosquitoes (Diptera: Culicidae) and cause serious illness in people community living in tropics. It is able to cause bundles of symptoms and may cause bleeding internally leading to death of the patient. Currently there is no immunization against dengue disorder but the most broadly utilized techniques for control of mosquitoes are usage of insecticides and biological control. Present study has been designed to observe and compare the consumption of dengue mosquito larvae by two anuran species, Common Skittering Frog (Euphlyctis cyanophlyctis) and Southeast Asian Toad (Duttaphrynus melanostictus) tadpoles inhabiting Rawalpindi and Islamabad. The tadpoles and dengue larvae were collected from ponds and pools using dip nets. Six trails were carried with anuran species of common skittering frog and Southeast Asian toad. Each trail was replicated thrice. The Kruskal-Wallis test present that the total number of consumed dengue larvae among various trials between two species (Common Skittering Frog and Southeast Asian Toad) did not differ-significantly (P=0.26) and (P=0.08), while the Wilcoxon test showed that the number of consumed dengue larvae by tadpoles of the two anuran species was P=0.08 which also did not differ significantly. Therefore it was concluded that the Southeast Asian toad is better in the consumption of dengue mosquito larvae as compared to common skittering frog.

Research paper thumbnail of The Study of Histopathological Changes in the Gonads of Frog

International Journal of Scientific & Engineering Research, Volume 5, 2014

ABSTRACT-Pesticides frequently premeditated a quick source for scheming weeds and insect pests bu... more ABSTRACT-Pesticides frequently premeditated a quick source for scheming weeds and insect pests but they cause noteworthy risk to non ‐targeted organisms. This present work includes susceptible species such as amphibians show venomous effect and death by extensive used pesticide lambda cyhalothrin in rice fields of Pakistan and this ecological alteration may initiate a reduced biodiversity, trophic cascades’ damage and ultimate harm to public health. Analysis has been made to assess the influence of various concentrations of lambda cyhalothrin on several histopathological constraints of gonads of frog. In this work 40 frogs were divided into four groups A (control), B(low lethal), C(median lethal) and D(high lethal) at no, 0.008,0.010 and 0.012mg/litre doses of different weight retaining frogs, respectively. The doses were given in water of aquarium mg/litre at 20th June,2014 and frogs in took them through cutaneous respiration and inhalation. Effect differs with frogs’ weight and morphological and histological alterations were observed in control group compared to treated group. At 2nd and 3rd day, frogs were pithed and observations concerning to morphology and histology of gonads were documented in all treatments, which incorporated discoloration and disruption of gonads and total degeneration of testis in less weighty frogs in morphological changes. Histopathological changes were foremost and included breaking of cyst walls, bunching of spermatocytes, necrosis of sertoli cells and sprinkled masses of decayed sperm bundles etc in testis and contraction in follicles, reduction in the number of oocytes, edema in stroma and formation of denatured yolk and necrosis etc. in ovaries which concluded that it has adverse affect on fertility of amphibians ultimately reducing their population size at all doses.
INDEX TERMS: Amphibian, Lambda-Cyhalothrin, Frog, Gonads, Rice fields, Decline, Pakistan