Ulisses Montarroyos | Universidade de Pernambuco - UPE (Brasil) (original) (raw)
Papers by Ulisses Montarroyos
PLoS ONE, 2013
Background: Given the lack of detailed studies on tuberculosis (TB) in patients on antiretroviral... more Background: Given the lack of detailed studies on tuberculosis (TB) in patients on antiretroviral treatment (ART) in South-East Asia, we aimed to determine the incidence and risk factors for early (after ≤6 months of ART) and late (after .6 months of ART) incident TB in Cambodia.
Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosi... more Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosis (TB) presents a series of challenges for treatment compliance for both providers and patients. We carried out this study to identify risk factors for default from TB treatment in people living with HIV.
BMC Infectious Diseases, 2011
Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosi... more Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosis (TB) presents a series of challenges for treatment compliance for both providers and patients. We carried out this study to identify risk factors for default from TB treatment in people living with HIV.
The American journal of tropical medicine and hygiene, 2009
This multicentric population-based study in Brazil is the first national effort to estimate the p... more This multicentric population-based study in Brazil is the first national effort to estimate the prevalence of hepatitis B (HBV) and risk factors in the capital cities of the Northeast, Central-West, and Federal Districts (2004-2005). Random multistage cluster sampling was used to select persons 13-69 years of age. Markers for HBV were tested by enzyme-linked immunosorbent assay. The HBV genotypes were determined by sequencing hepatitis B surface antigen (HBsAg). Multivariate analyses and simple catalytic model were performed. Overall, 7,881 persons were included; < 70% were not vaccinated. Positivity for HBsAg was less than 1% among non-vaccinated persons and genotypes A, D, and F co-circulated. The incidence of infection increased with age with similar force of infection in all regions. Males and persons having initiated sexual activity were associated with HBV infection in the two settings; healthcare jobs and prior hospitalization were risk factors in the Federal District. Our...
Journal of the International Association of Physicians in AIDS Care (Chicago, Ill. : 2002)
The effects of HIV/AIDS and antiretroviral drugs on vitamin D metabolism are still mostly unknown... more The effects of HIV/AIDS and antiretroviral drugs on vitamin D metabolism are still mostly unknown. This was a cross-sectional study to estimate the prevalence of vitamin D deficiency and identify its association with the clinical and metabolic parameters among 214 HIV-positive female patients on antiretroviral therapy (ART) in Brazil. The prevalence of vitamin D deficiency (< 30 ng/ml) was 40.65% (87/214). Hypercholesterolemia, high LDL-c, duration of use of current antiretroviral regimen, hypertriglyceridemia, body mass index, age, hypertension, time with AIDS ≥ 10 years and hyperglycemia were selected for multivariate analysis (p < 0.20). After this analysis, hypercholesterolemia and use of current antiretroviral regimen ≥ 3 years remained independently associated with vitamin D deficiency. There was an inverse statistically significant correlation between total cholesterol and serum 25(OH)D levels. High prevalence of vitamin D deficiency was found among HIV-positive women o...
Jornal Brasileiro de Pneumologia, 2006
Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle... more Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle 535 Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle* Characteristics of elderly tuberculosis patients in Recife, Brazil: a contribution to the tuberculosis control program ZILDA DO REGO CAVALCANTI 1 , MARIA DE FÁTIMA PESSOA MILITÃO DE ALBUQUERQUE 2 , ANTÔNIO ROBERTO LEITE CAMPELO 2 , RICARDO XIMENES 3 , ULISSES MONTARROYOS 4 , MARIANNE KAREL AMARAL VERÇOSA 5 RESUMO Objetivo: Descrever as características demográficas, de hábitos de vida, socioeconômicas, clínico-epidemiológicas e de acesso aos serviços de saúde de idosos com tuberculose, diagnosticados e tratados no Recife (PE), e compará-las com os adultos jovens em mesmas condições. Métodos: Utilizou-se uma estratégia de análise do tipo caso-controle em uma coorte de pacientes com tuberculose, atendidos nas unidades de saúde pública do Recife no período de maio de 2001 a julho de 2003. Resultados: Foram incluídos no estudo 1.127 pacientes, 136 idosos (casos) e 991 adultos jovens (controles). Nos dois grupos o sexo prevalente foi o masculino e a forma da doença a pulmonar. O etilismo foi mais freqüente entre os controles e o analfabetismo entre os casos. Os idosos queixaram-se menos de tosse, sudorese e dor torácica. A sorologia para o vírus da imunodeficiência humana foi realizada em apenas 29 pacientes (2,6%). Os controles tiveram maior percentual de positividade nos exames de baciloscopia e cultura. Ambos os grupos tiveram que procurar mais de dois serviços de saúde e passaram-se mais de dois meses até que se fizesse o diagnóstico da doença. Os idosos tiveram maiores índices de cura e óbito, e abandonaram menos o tratamento. Conclusão: Na população estudada, os idosos apresentaram menos tosse, sudorese noturna e dor torácica, menor positividade nos exames complementares e maior mortalidade. Devem constituir um grupo com abordagem especial dos serviços de saúde pública.
![Research paper thumbnail of [Hematological and histopathological evaluation of BALB/c and C57BL/6 mice exposed to Cytoplasmic Repetitive Antigen and Flagellar Repetitive Antigen recombinant antigens of Trypanosoma cruzi]](https://attachments.academia-assets.com/44036524/thumbnails/1.jpg)
](Revista da Sociedade Brasileira de Medicina Tropical
The Cytoplasmic Repetitive Antigen and Flagellar Repetitive Antigen recombinant antigens of Trypa... more The Cytoplasmic Repetitive Antigen and Flagellar Repetitive Antigen recombinant antigens of Trypanosoma cruzi were inoculated into BALB/c and C57BL/6 mice and its effects evaluated at hematological and histopathological levels. The results showed that the histological pattern of the organs and the hematological profile of mice were not modified suggesting that these antigens are not harmful for the animal.
Revista da Sociedade Brasileira de Medicina Tropical
Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypan... more Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.
BMC medical research methodology, 2007
In most case control studies the hardest decision is the choice of the control group, as in the i... more In most case control studies the hardest decision is the choice of the control group, as in the ideal control group the proportion exposed is the same as in the population that produced the cases. A comparison of two control groups in a case control study of the efficacy of BCG revaccination. One group was selected from subjects presenting to the heath unit the case attended for routine prevention and care; the second group was selected from the neighbourhood of cases. All Health Units from which controls were selected offered BCG revaccination. Efficacy estimated in a randomized control trial of BCG revaccination was used to establish that the neighbourhood control group was the one that gave unbiased results. The proportion of controls with scars indicating BCG revaccination was higher among the control group selected from Health Unit attenders than among neighbourhood controls. This excess was not removed after control for social variables and history of exposure to tuberculosis,...
Revista Da Sociedade Brasileira De Medicina Tropical - REV SOC BRAS MED TROP, 2003
Resumo Os antígenos recombinantes Cytoplasmic Repetitive Antigen e Flagellar Repetitive Antigen d... more Resumo Os antígenos recombinantes Cytoplasmic Repetitive Antigen e Flagellar Repetitive Antigen de Trypanosoma cruzi foram inoculados em camundongos BALB/c e C57BL/6 e o seu efeito avaliado a nível hematológico e histopatológico. Os resultados mostraram que o padrão histológico normal dos órgãos e o perfil hematológico dos camundongos não foram modificados sugerindo que esses antígenos não parecem causar dano ao animal. Palavras-chaves: Trypanosoma cruzi. Antígenos recombinantes. Perfis hematológico e histopatológico.
Reproductive Health, 2011
To evaluate the effectiveness and safety of polyunsaturated fatty acids for the treatment of the ... more To evaluate the effectiveness and safety of polyunsaturated fatty acids for the treatment of the premenstrual syndrome (PMS) using a graded symptom scale and to assess the effect of this treatment on basal plasma levels of prolactin and total cholesterol. A randomized, double-blind, placebo-controlled study was conducted with 120 women with PMS divided into three groups and treated with 1 or 2 grams of the medication or placebo. Symptoms were recorded over a 6-month period using the Prospective Record of the Impact and Severity of Menstruation (PRISM) calendar. Total cholesterol and prolactin levels were measured. Analysis of variance (ANOVA), Pearson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s chi-square test, Wilcoxon&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s nonparametric signed-rank test for paired samples and the Mann-Whitney nonparametric test for independent samples were used in the statistical analysis. There were no differences in age, marital status, schooling or ethnicity between the groups. In the group treated with 1 gram of the medication, a significant reduction was found when the median PRISM score recorded in the luteal phase at baseline (99) was compared with the median score recorded in the 3rd month (58) and in the 6th month of evaluation (35). In the 2-gram group, these differences were even more significant (baseline score: 98; 3rd month: 48; 6th month: 28). In the placebo group, there was a significant reduction at the 3rd but not at the 6th month (baseline: 96.5; 3rd month: 63.5; 6th month: 62). The difference between the phases of the menstrual cycle was greater in the 2-gram group compared to the group treated with 1 gram of the medication. There were no statistically significant differences in prolactin or total cholesterol levels between baseline values and those recorded after six months of treatment. The difference between the groups using the medication and the placebo group with respect to the improvement in symptomatology appears to indicate the effectiveness of the drug. Improvement in symptoms was higher when the 2-gram dose was used. This medication was not associated with any changes in prolactin or total cholesterol levels in these women.
PLoS ONE, 2014
Background: The challenge of diagnosing smear-negative pulmonary TB (tuberculosis) in people livi... more Background: The challenge of diagnosing smear-negative pulmonary TB (tuberculosis) in people living with HIV justifies the use of instruments other than the smear test for diagnosing the disease. Considering the clinical-radiological similarities of TB amongst HIV-infected adults and children, the proposal of this study was to assess the accuracy of a scoring system used to diagnose smear-negative pulmonary TB in children and adolescents, in HIV-infected adults suspected of having smearnegative pulmonary TB.
PLoS ONE, 2014
Background: The measurement of CD4+ T-cell (CD4) counts is a strong predictor of progression to A... more Background: The measurement of CD4+ T-cell (CD4) counts is a strong predictor of progression to AIDS and a means of monitoring antiviral therapy (ART). The success or failure of controlling virus levels in untreated patients or those taking ART may be associated with treatment adherence, habits, correlated infections unrelated to HIV, cancer, immunosuppressive drugs; as well as socio-economic and psychosocial aspects and access to healthcare. The aim of the present study was to identify, using a multilevel model, the factors related to the variations of CD4 counts over time, in patients living with HIV.
Memórias do Instituto Oswaldo Cruz, 2003
In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c ... more In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagelar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of all IgG isotypes, mainly IgG3 and IgG1. FRAimmunization elicited only high levels of IgG1.
Memórias do Instituto Oswaldo Cruz, 2002
In the present communication we analyzed the levels of IgG1, IgG2, IgG3, IgG4 and IgE isotypes to... more In the present communication we analyzed the levels of IgG1, IgG2, IgG3, IgG4 and IgE isotypes to soluble egg antigen of Schistosoma mansoni by ELISA in individuals from an endemic area for schistosomiasis in Northeast Brazil. The analysis was performed before and after treatment to evaluate the age-dependent pattern, and to identify differences in the reactivities to antigens. Our results suggest that schistosomiasis treatment would not interfere with this sort of immune response.
Memórias do Instituto Oswaldo Cruz, 2004
The purpose of this study was to examine the circulating filarial antigen (CFA) detected by the m... more The purpose of this study was to examine the circulating filarial antigen (CFA) detected by the monoclonal antibody (mAb) Og4C3-ELISA in paired samples of serum and hydrocele fluid from 104 men with hydrocele, living in an endemic area of Wuchereria bancrofti. Nocturnal blood specimens were filtered and examined for microfilariae (MF) and ultrasound was used in order to identify the presence of adult worms (the filaria dance sign -FDS) in the lymphatic vessels of the scrotal area. Four groups were selected according to their parasitological status: group I -71 MF _ and FDS _ ; group II -21 MF+ and FDS+; group III -10 MF-and FDS+ and group IV-2 MF+ and FDS _ . CFA was identified simultaneously (fluid and serum) in 11 (15.5%), 21 (100%), 3 (30%), and 1 (50%) in groups I, II, III, and IV, respectively. In despite of high CFA+ level (antigen Og4C3) units/ml, the Geometrical Mean (GM) = 2696) in the sera of these 36/104 paired samples, when compared to the hydrocele fluid, (GM = 1079), showed a very good correlation between the CFA level in the serum and CFA level in the fluid (r = 0.731). CFA level in the serum of the 23 microfilaremics (groups II and IV) was extremely high (GM = 4189) and was correlated with MF density (r = 0.442). These findings report for the first time the potential alternative use of the hydrocele fluid to investigate CFA using the mAb Og4C3-ELISA.
International Journal of Infectious Diseases, 2010
International Journal of Infectious Diseases, 2010
The study sites were classified as areas with intermediate endemicity area for hepatitis A infect... more The study sites were classified as areas with intermediate endemicity area for hepatitis A infection. Differences in age trends of infection were detected among settings. This multilevel model allowed for quantification of contextual predictors of hepatitis A infection in urban areas.
International Journal of Infectious Diseases, 2010
Abstracts e235 outcome. Additionally, education and sewage disposal service variables that were c... more Abstracts e235 outcome. Additionally, education and sewage disposal service variables that were collected through household-level interviews or retrieved from IBGE census tract data were associated with HAV infection in all sites.
International Journal of Infectious Diseases, 2010
Abstracts nucleos(t)ide analogues. Patients were evaluated based on virologic and serologic respo... more Abstracts nucleos(t)ide analogues. Patients were evaluated based on virologic and serologic response to therapy, and were classified as patients with a detectable level of HBV DNA and undetectable HBV DNA (<1.08 log copies/ml). The mutations associated with HBV drug resistance were investigated in patients with detectable HBV DNA. Due to resistance, in this group of patients treatment was change.
PLoS ONE, 2013
Background: Given the lack of detailed studies on tuberculosis (TB) in patients on antiretroviral... more Background: Given the lack of detailed studies on tuberculosis (TB) in patients on antiretroviral treatment (ART) in South-East Asia, we aimed to determine the incidence and risk factors for early (after ≤6 months of ART) and late (after .6 months of ART) incident TB in Cambodia.
Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosi... more Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosis (TB) presents a series of challenges for treatment compliance for both providers and patients. We carried out this study to identify risk factors for default from TB treatment in people living with HIV.
BMC Infectious Diseases, 2011
Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosi... more Background: Concomitant treatment of Human Immunodeficiency Virus (HIV) infection and tuberculosis (TB) presents a series of challenges for treatment compliance for both providers and patients. We carried out this study to identify risk factors for default from TB treatment in people living with HIV.
The American journal of tropical medicine and hygiene, 2009
This multicentric population-based study in Brazil is the first national effort to estimate the p... more This multicentric population-based study in Brazil is the first national effort to estimate the prevalence of hepatitis B (HBV) and risk factors in the capital cities of the Northeast, Central-West, and Federal Districts (2004-2005). Random multistage cluster sampling was used to select persons 13-69 years of age. Markers for HBV were tested by enzyme-linked immunosorbent assay. The HBV genotypes were determined by sequencing hepatitis B surface antigen (HBsAg). Multivariate analyses and simple catalytic model were performed. Overall, 7,881 persons were included; < 70% were not vaccinated. Positivity for HBsAg was less than 1% among non-vaccinated persons and genotypes A, D, and F co-circulated. The incidence of infection increased with age with similar force of infection in all regions. Males and persons having initiated sexual activity were associated with HBV infection in the two settings; healthcare jobs and prior hospitalization were risk factors in the Federal District. Our...
Journal of the International Association of Physicians in AIDS Care (Chicago, Ill. : 2002)
The effects of HIV/AIDS and antiretroviral drugs on vitamin D metabolism are still mostly unknown... more The effects of HIV/AIDS and antiretroviral drugs on vitamin D metabolism are still mostly unknown. This was a cross-sectional study to estimate the prevalence of vitamin D deficiency and identify its association with the clinical and metabolic parameters among 214 HIV-positive female patients on antiretroviral therapy (ART) in Brazil. The prevalence of vitamin D deficiency (< 30 ng/ml) was 40.65% (87/214). Hypercholesterolemia, high LDL-c, duration of use of current antiretroviral regimen, hypertriglyceridemia, body mass index, age, hypertension, time with AIDS ≥ 10 years and hyperglycemia were selected for multivariate analysis (p < 0.20). After this analysis, hypercholesterolemia and use of current antiretroviral regimen ≥ 3 years remained independently associated with vitamin D deficiency. There was an inverse statistically significant correlation between total cholesterol and serum 25(OH)D levels. High prevalence of vitamin D deficiency was found among HIV-positive women o...
Jornal Brasileiro de Pneumologia, 2006
Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle... more Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle 535 Características da tuberculose em idosos no Recife (PE): contribuição para o programa de controle* Characteristics of elderly tuberculosis patients in Recife, Brazil: a contribution to the tuberculosis control program ZILDA DO REGO CAVALCANTI 1 , MARIA DE FÁTIMA PESSOA MILITÃO DE ALBUQUERQUE 2 , ANTÔNIO ROBERTO LEITE CAMPELO 2 , RICARDO XIMENES 3 , ULISSES MONTARROYOS 4 , MARIANNE KAREL AMARAL VERÇOSA 5 RESUMO Objetivo: Descrever as características demográficas, de hábitos de vida, socioeconômicas, clínico-epidemiológicas e de acesso aos serviços de saúde de idosos com tuberculose, diagnosticados e tratados no Recife (PE), e compará-las com os adultos jovens em mesmas condições. Métodos: Utilizou-se uma estratégia de análise do tipo caso-controle em uma coorte de pacientes com tuberculose, atendidos nas unidades de saúde pública do Recife no período de maio de 2001 a julho de 2003. Resultados: Foram incluídos no estudo 1.127 pacientes, 136 idosos (casos) e 991 adultos jovens (controles). Nos dois grupos o sexo prevalente foi o masculino e a forma da doença a pulmonar. O etilismo foi mais freqüente entre os controles e o analfabetismo entre os casos. Os idosos queixaram-se menos de tosse, sudorese e dor torácica. A sorologia para o vírus da imunodeficiência humana foi realizada em apenas 29 pacientes (2,6%). Os controles tiveram maior percentual de positividade nos exames de baciloscopia e cultura. Ambos os grupos tiveram que procurar mais de dois serviços de saúde e passaram-se mais de dois meses até que se fizesse o diagnóstico da doença. Os idosos tiveram maiores índices de cura e óbito, e abandonaram menos o tratamento. Conclusão: Na população estudada, os idosos apresentaram menos tosse, sudorese noturna e dor torácica, menor positividade nos exames complementares e maior mortalidade. Devem constituir um grupo com abordagem especial dos serviços de saúde pública.
Revista da Sociedade Brasileira de Medicina Tropical
The Cytoplasmic Repetitive Antigen and Flagellar Repetitive Antigen recombinant antigens of Trypa... more The Cytoplasmic Repetitive Antigen and Flagellar Repetitive Antigen recombinant antigens of Trypanosoma cruzi were inoculated into BALB/c and C57BL/6 mice and its effects evaluated at hematological and histopathological levels. The results showed that the histological pattern of the organs and the hematological profile of mice were not modified suggesting that these antigens are not harmful for the animal.
Revista da Sociedade Brasileira de Medicina Tropical
Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypan... more Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [ H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.
BMC medical research methodology, 2007
In most case control studies the hardest decision is the choice of the control group, as in the i... more In most case control studies the hardest decision is the choice of the control group, as in the ideal control group the proportion exposed is the same as in the population that produced the cases. A comparison of two control groups in a case control study of the efficacy of BCG revaccination. One group was selected from subjects presenting to the heath unit the case attended for routine prevention and care; the second group was selected from the neighbourhood of cases. All Health Units from which controls were selected offered BCG revaccination. Efficacy estimated in a randomized control trial of BCG revaccination was used to establish that the neighbourhood control group was the one that gave unbiased results. The proportion of controls with scars indicating BCG revaccination was higher among the control group selected from Health Unit attenders than among neighbourhood controls. This excess was not removed after control for social variables and history of exposure to tuberculosis,...
Revista Da Sociedade Brasileira De Medicina Tropical - REV SOC BRAS MED TROP, 2003
Resumo Os antígenos recombinantes Cytoplasmic Repetitive Antigen e Flagellar Repetitive Antigen d... more Resumo Os antígenos recombinantes Cytoplasmic Repetitive Antigen e Flagellar Repetitive Antigen de Trypanosoma cruzi foram inoculados em camundongos BALB/c e C57BL/6 e o seu efeito avaliado a nível hematológico e histopatológico. Os resultados mostraram que o padrão histológico normal dos órgãos e o perfil hematológico dos camundongos não foram modificados sugerindo que esses antígenos não parecem causar dano ao animal. Palavras-chaves: Trypanosoma cruzi. Antígenos recombinantes. Perfis hematológico e histopatológico.
Reproductive Health, 2011
To evaluate the effectiveness and safety of polyunsaturated fatty acids for the treatment of the ... more To evaluate the effectiveness and safety of polyunsaturated fatty acids for the treatment of the premenstrual syndrome (PMS) using a graded symptom scale and to assess the effect of this treatment on basal plasma levels of prolactin and total cholesterol. A randomized, double-blind, placebo-controlled study was conducted with 120 women with PMS divided into three groups and treated with 1 or 2 grams of the medication or placebo. Symptoms were recorded over a 6-month period using the Prospective Record of the Impact and Severity of Menstruation (PRISM) calendar. Total cholesterol and prolactin levels were measured. Analysis of variance (ANOVA), Pearson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s chi-square test, Wilcoxon&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s nonparametric signed-rank test for paired samples and the Mann-Whitney nonparametric test for independent samples were used in the statistical analysis. There were no differences in age, marital status, schooling or ethnicity between the groups. In the group treated with 1 gram of the medication, a significant reduction was found when the median PRISM score recorded in the luteal phase at baseline (99) was compared with the median score recorded in the 3rd month (58) and in the 6th month of evaluation (35). In the 2-gram group, these differences were even more significant (baseline score: 98; 3rd month: 48; 6th month: 28). In the placebo group, there was a significant reduction at the 3rd but not at the 6th month (baseline: 96.5; 3rd month: 63.5; 6th month: 62). The difference between the phases of the menstrual cycle was greater in the 2-gram group compared to the group treated with 1 gram of the medication. There were no statistically significant differences in prolactin or total cholesterol levels between baseline values and those recorded after six months of treatment. The difference between the groups using the medication and the placebo group with respect to the improvement in symptomatology appears to indicate the effectiveness of the drug. Improvement in symptoms was higher when the 2-gram dose was used. This medication was not associated with any changes in prolactin or total cholesterol levels in these women.
PLoS ONE, 2014
Background: The challenge of diagnosing smear-negative pulmonary TB (tuberculosis) in people livi... more Background: The challenge of diagnosing smear-negative pulmonary TB (tuberculosis) in people living with HIV justifies the use of instruments other than the smear test for diagnosing the disease. Considering the clinical-radiological similarities of TB amongst HIV-infected adults and children, the proposal of this study was to assess the accuracy of a scoring system used to diagnose smear-negative pulmonary TB in children and adolescents, in HIV-infected adults suspected of having smearnegative pulmonary TB.
PLoS ONE, 2014
Background: The measurement of CD4+ T-cell (CD4) counts is a strong predictor of progression to A... more Background: The measurement of CD4+ T-cell (CD4) counts is a strong predictor of progression to AIDS and a means of monitoring antiviral therapy (ART). The success or failure of controlling virus levels in untreated patients or those taking ART may be associated with treatment adherence, habits, correlated infections unrelated to HIV, cancer, immunosuppressive drugs; as well as socio-economic and psychosocial aspects and access to healthcare. The aim of the present study was to identify, using a multilevel model, the factors related to the variations of CD4 counts over time, in patients living with HIV.
Memórias do Instituto Oswaldo Cruz, 2003
In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c ... more In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagelar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of all IgG isotypes, mainly IgG3 and IgG1. FRAimmunization elicited only high levels of IgG1.
Memórias do Instituto Oswaldo Cruz, 2002
In the present communication we analyzed the levels of IgG1, IgG2, IgG3, IgG4 and IgE isotypes to... more In the present communication we analyzed the levels of IgG1, IgG2, IgG3, IgG4 and IgE isotypes to soluble egg antigen of Schistosoma mansoni by ELISA in individuals from an endemic area for schistosomiasis in Northeast Brazil. The analysis was performed before and after treatment to evaluate the age-dependent pattern, and to identify differences in the reactivities to antigens. Our results suggest that schistosomiasis treatment would not interfere with this sort of immune response.
Memórias do Instituto Oswaldo Cruz, 2004
The purpose of this study was to examine the circulating filarial antigen (CFA) detected by the m... more The purpose of this study was to examine the circulating filarial antigen (CFA) detected by the monoclonal antibody (mAb) Og4C3-ELISA in paired samples of serum and hydrocele fluid from 104 men with hydrocele, living in an endemic area of Wuchereria bancrofti. Nocturnal blood specimens were filtered and examined for microfilariae (MF) and ultrasound was used in order to identify the presence of adult worms (the filaria dance sign -FDS) in the lymphatic vessels of the scrotal area. Four groups were selected according to their parasitological status: group I -71 MF _ and FDS _ ; group II -21 MF+ and FDS+; group III -10 MF-and FDS+ and group IV-2 MF+ and FDS _ . CFA was identified simultaneously (fluid and serum) in 11 (15.5%), 21 (100%), 3 (30%), and 1 (50%) in groups I, II, III, and IV, respectively. In despite of high CFA+ level (antigen Og4C3) units/ml, the Geometrical Mean (GM) = 2696) in the sera of these 36/104 paired samples, when compared to the hydrocele fluid, (GM = 1079), showed a very good correlation between the CFA level in the serum and CFA level in the fluid (r = 0.731). CFA level in the serum of the 23 microfilaremics (groups II and IV) was extremely high (GM = 4189) and was correlated with MF density (r = 0.442). These findings report for the first time the potential alternative use of the hydrocele fluid to investigate CFA using the mAb Og4C3-ELISA.
International Journal of Infectious Diseases, 2010
International Journal of Infectious Diseases, 2010
The study sites were classified as areas with intermediate endemicity area for hepatitis A infect... more The study sites were classified as areas with intermediate endemicity area for hepatitis A infection. Differences in age trends of infection were detected among settings. This multilevel model allowed for quantification of contextual predictors of hepatitis A infection in urban areas.
International Journal of Infectious Diseases, 2010
Abstracts e235 outcome. Additionally, education and sewage disposal service variables that were c... more Abstracts e235 outcome. Additionally, education and sewage disposal service variables that were collected through household-level interviews or retrieved from IBGE census tract data were associated with HAV infection in all sites.
International Journal of Infectious Diseases, 2010
Abstracts nucleos(t)ide analogues. Patients were evaluated based on virologic and serologic respo... more Abstracts nucleos(t)ide analogues. Patients were evaluated based on virologic and serologic response to therapy, and were classified as patients with a detectable level of HBV DNA and undetectable HBV DNA (<1.08 log copies/ml). The mutations associated with HBV drug resistance were investigated in patients with detectable HBV DNA. Due to resistance, in this group of patients treatment was change.