William Denney | University of Pennsylvania (original) (raw)
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Papers by William Denney
Journal of the American Society of Nephrology, Nov 1, 2023
Clinical and translational science, May 1, 2024
Journal of the American Society of Nephrology, Oct 1, 2021
Cell host & microbe, Feb 1, 2024
Frontiers in neuroscience, Feb 1, 2024
Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a deter... more Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIb␣ and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/m 2 at wall shear rates of 100, 500, and 1000 s ؊1 , respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood (؎ 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow.
European Journal of Pain, 2019
BackgroundAlthough reproducibility is considered essential for any method used in scientific rese... more BackgroundAlthough reproducibility is considered essential for any method used in scientific research, it is investigated only rarely; thus, strikingly little has been published regarding the reproducibility of evoked pain models involving human subjects. Here, we studied the reproducibility of a battery of evoked pain models for demonstrating the analgesic effects of two analgesic compounds.MethodsA total of 81 healthy subjects participated in four studies involving a battery of evoked pain tests in which mechanical, thermal and electrical stimuli were used to measure pain detection and tolerance thresholds. Pharmacodynamic outcome variables were analysed using a mixed model analysis of variance, and a coefficient of variation was calculated by dividing the standard deviation by the least squares means.ResultsA total of 76 subjects completed the studies. After being administered pregabalin, the subjects’ pain tolerance thresholds in the cold pressor and pressure stimulation tests w...
Nature Metabolism
In the version of this article initially published, in the Fig. 3a,b y-axis unit labels now readi... more In the version of this article initially published, in the Fig. 3a,b y-axis unit labels now reading "(h μmol l-1), " there was an error originally where "-1 " followed "h. " The labels have been replaced in the HTML and PDF versions of the article.
Molecular Genetics and Metabolism, 2022
Journal of Hepatology, 2018
SAT-285): SYNB1020 genetic modifications. (1) argR repressor gene deleted, (2) argA gene replaced... more SAT-285): SYNB1020 genetic modifications. (1) argR repressor gene deleted, (2) argA gene replaced with a feedback-resistant version, (3) Control of argA gene by anaerobic promoter (fnr), (4) thyA gene deleted to confer auxotrophy.
Journal of the American Society of Nephrology, Nov 1, 2023
Clinical and translational science, May 1, 2024
Journal of the American Society of Nephrology, Oct 1, 2021
Cell host & microbe, Feb 1, 2024
Frontiers in neuroscience, Feb 1, 2024
Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a deter... more Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIb␣ and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/m 2 at wall shear rates of 100, 500, and 1000 s ؊1 , respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood (؎ 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow.
European Journal of Pain, 2019
BackgroundAlthough reproducibility is considered essential for any method used in scientific rese... more BackgroundAlthough reproducibility is considered essential for any method used in scientific research, it is investigated only rarely; thus, strikingly little has been published regarding the reproducibility of evoked pain models involving human subjects. Here, we studied the reproducibility of a battery of evoked pain models for demonstrating the analgesic effects of two analgesic compounds.MethodsA total of 81 healthy subjects participated in four studies involving a battery of evoked pain tests in which mechanical, thermal and electrical stimuli were used to measure pain detection and tolerance thresholds. Pharmacodynamic outcome variables were analysed using a mixed model analysis of variance, and a coefficient of variation was calculated by dividing the standard deviation by the least squares means.ResultsA total of 76 subjects completed the studies. After being administered pregabalin, the subjects’ pain tolerance thresholds in the cold pressor and pressure stimulation tests w...
Nature Metabolism
In the version of this article initially published, in the Fig. 3a,b y-axis unit labels now readi... more In the version of this article initially published, in the Fig. 3a,b y-axis unit labels now reading "(h μmol l-1), " there was an error originally where "-1 " followed "h. " The labels have been replaced in the HTML and PDF versions of the article.
Molecular Genetics and Metabolism, 2022
Journal of Hepatology, 2018
SAT-285): SYNB1020 genetic modifications. (1) argR repressor gene deleted, (2) argA gene replaced... more SAT-285): SYNB1020 genetic modifications. (1) argR repressor gene deleted, (2) argA gene replaced with a feedback-resistant version, (3) Control of argA gene by anaerobic promoter (fnr), (4) thyA gene deleted to confer auxotrophy.