Vladislav Kolarčik | Univerzita Pavla Jozefa Šafárika (original) (raw)

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Papers by Vladislav Kolarčik

Research paper thumbnail of Biologia palynology onosma

Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy w... more Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5-17.5 × 8.0-14.5 µm), and O. pseudoarenaria the largest grains in average (15.3-20.5 × 12.3-16.3 µm).

Research paper thumbnail of Palynological analysis of five selected Onosma taxa

Biologia, 2008

Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy w... more Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5–17.5 × 8.0–14.5 µm), and O. pseudoarenaria the largest grains in average (15.3–20.5 × 12.3–16.3 µm). Pollen grains of all 5 species are small sized, 3-syncolporate, subprolate, heteropolar, with ovate equatorial outlines and circular to rounded triangular polar outlines; the tectum is microechinate. A positive correlation is found between pollen size and chromosome number. The value of pollen characters for taxonomic purposes and the position of the taxa studied within the genus Onosma are discussed.

Research paper thumbnail of Systematics and evolutionary history of the Asterotricha group of the genus Onosma (Boraginaceae) in central and southern Europe inferred from AFLP and nrDNA ITS data

Plant Systematics and Evolution, 2010

Onosma is a species-rich genus with complicated patterns of morphological and karyological variat... more Onosma is a species-rich genus with complicated patterns of morphological and karyological variation, and controversial taxonomic treatments. In the present study we focused on the Asterotricha group, one of three major infrageneric groups, in the area of central and southern Europe. Ribosomal internal transcribed spacer (ITS) sequences and amplified fragment length polymorphism (AFLP) markers were used to assess species differentiation and relationships, and to gain insight into the evolutionary history of the group. ITS data supported the monophyly of the asterotrichous taxa. Six genetically differentiated entities were resolved by AFLP markers, corresponding to O. echioides, O. heterophylla s.l., O. thracica including O. rigida, O. malkarmayorum, O. taurica, and O. stellulata. Onosma stellulata is identified as the most differentiated lineage within the Asterotricha group, which is consistent with its unique chromosome number. Within O. heterophylla s.l., recognition of O. viridis as a species distinct from O. heterophylla s.str. is supported. On the other hand, O. tornensis appears to be conspecific with O. viridis. Inferring from the patterns of genetic diversity and divergence, the evolutionary history of O. heterophylla s.l. is discussed. Genetic variation within the polymorphic Apennine–Dalmatian O. echioides is almost continuous and shaped by isolation by distance, thus none of the previous intraspecific taxonomic concepts are valid. The assumed hybrid origin of O. malkarmayorum is supported, and its affinity to potential ancestors, O. heterophylla s.l. and O. thracica incl. O. rigida, is indicated. Future studies of asterotrichous Onosma species should focus on diversity centers in the eastern Mediterranean and Asia Minor, to build up a comprehensive taxonomic concept of the group and to clarify its evolution.

Research paper thumbnail of Biologia palynology onosma

Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy w... more Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5-17.5 × 8.0-14.5 µm), and O. pseudoarenaria the largest grains in average (15.3-20.5 × 12.3-16.3 µm).

Research paper thumbnail of Palynological analysis of five selected Onosma taxa

Biologia, 2008

Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy w... more Pollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5–17.5 × 8.0–14.5 µm), and O. pseudoarenaria the largest grains in average (15.3–20.5 × 12.3–16.3 µm). Pollen grains of all 5 species are small sized, 3-syncolporate, subprolate, heteropolar, with ovate equatorial outlines and circular to rounded triangular polar outlines; the tectum is microechinate. A positive correlation is found between pollen size and chromosome number. The value of pollen characters for taxonomic purposes and the position of the taxa studied within the genus Onosma are discussed.

Research paper thumbnail of Systematics and evolutionary history of the Asterotricha group of the genus Onosma (Boraginaceae) in central and southern Europe inferred from AFLP and nrDNA ITS data

Plant Systematics and Evolution, 2010

Onosma is a species-rich genus with complicated patterns of morphological and karyological variat... more Onosma is a species-rich genus with complicated patterns of morphological and karyological variation, and controversial taxonomic treatments. In the present study we focused on the Asterotricha group, one of three major infrageneric groups, in the area of central and southern Europe. Ribosomal internal transcribed spacer (ITS) sequences and amplified fragment length polymorphism (AFLP) markers were used to assess species differentiation and relationships, and to gain insight into the evolutionary history of the group. ITS data supported the monophyly of the asterotrichous taxa. Six genetically differentiated entities were resolved by AFLP markers, corresponding to O. echioides, O. heterophylla s.l., O. thracica including O. rigida, O. malkarmayorum, O. taurica, and O. stellulata. Onosma stellulata is identified as the most differentiated lineage within the Asterotricha group, which is consistent with its unique chromosome number. Within O. heterophylla s.l., recognition of O. viridis as a species distinct from O. heterophylla s.str. is supported. On the other hand, O. tornensis appears to be conspecific with O. viridis. Inferring from the patterns of genetic diversity and divergence, the evolutionary history of O. heterophylla s.l. is discussed. Genetic variation within the polymorphic Apennine–Dalmatian O. echioides is almost continuous and shaped by isolation by distance, thus none of the previous intraspecific taxonomic concepts are valid. The assumed hybrid origin of O. malkarmayorum is supported, and its affinity to potential ancestors, O. heterophylla s.l. and O. thracica incl. O. rigida, is indicated. Future studies of asterotrichous Onosma species should focus on diversity centers in the eastern Mediterranean and Asia Minor, to build up a comprehensive taxonomic concept of the group and to clarify its evolution.