Devi Maya Sofa | UPN "Veteran" Jawa Timur (original) (raw)
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Address: Surabaya
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Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to a... more Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to available clinical treatments. The herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) system produces the selective elimination of HSVtk-positive cells and is a candidate for preclinical testing against glioblastoma via its ability to regulate proliferation and differentiation. Therefore, in this study, we aimed to establish a plasmid encoding the HSVtk/GCV system driven by a glial fibrillary acidic protein (GFAP) promoter and verify its possibility of neural differentiation of glioblastoma cell line under the GCV challenge. Four stable clones-N2A-pCMV-HSVtk, N2A-pGFAP-HSVtk, U251-pCMV-HSVtk, and U251-pGFAP-HSVtk-were established from neuronal N2A and glioblastoma U251 cell lines. In vitro GCV sensitivity was assessed by MTT assay for monitoring time-and dosage-dependent cytotoxicity. The capability for neural differentiation in stable glioblastoma clones during GCV treatment was assessed by performing immunocytochemistry for nestin, GFAP, and βIII-tubulin. Under GFAP promoter control, the U251 stable clone exhibited GCV sensitivity, while the neuronal N2A clones were nonreactive. During GCV treatment, cells underwent apoptosis on day 3 and dying cells were identified after day 5. Nestin was increasingly expressed in surviving cells, indicating that the population of neural stem-like cells was enriched. Lower levels of GFAP expression were detected in surviving cells. Furthermore, βIII-tubulin-positive neuron-like cells were identified after GCV treatment. This study established pGFAP-HSVtk-P2A-EGFP plasmids that successfully ablated GFAP-positive glioblastoma cells, but left neuronal N2A cells intact. These data suggest that the neural differentiation of glioblastoma cells can be promoted by treatment with the HSVtk/GCV system.
RELASI : JURNAL EKONOMI, 2021
Academic fraud can occur in university because it is influenced by various underlying factors, bo... more Academic fraud can occur in university because it is influenced by various underlying factors, both internally and externally. The purpose of holding this study was to determine whether the pressure, opportunity, rationalization, and abilities possessed by students significantly influence the occurrence of academic fraud in university. This research was conducted on 92 students of the Accounting Major in East Java "Veteran" National Development University by filling out a questionnaire via Google Form. The results of data collection were processed statistically using SmartPLS. The results of data analysis show that pressure, rationalization, and ability have a significant effect on academic fraud, while opportunities do not significantly influence students because lecturers always provide objective assessments to students, thereby reducing the chance of academic fraud. Keywords: Diamond Fraud Theory, Academic Fraud
JEA17: Jurnal Ekonomi Akuntansi, 2018
ABSTRACTThis study aims to describe how the management of the Village Fund Allocation and the Vil... more ABSTRACTThis study aims to describe how the management of the Village Fund Allocation and the Village Fund implemented in Tulungrejo districts Donomulyo Malang district, which is in the process of planning, implementation and accountability if it is based on procedures and rules, whether the principles princip management has been able to be realized. And supporting factors and inhibitors that affect the management of the Village Fund Allocation and the village fund. In this research, the calculation to determine the amount of the management of the funds used for the fields in rural development. The results of data analysis showed that the allocation of funds and funds rural village in the village which Tulungrejo in use for four areas of rural development is the empowerment of the community amounted to 81%, the field of administration of the village of 47.16%, 41.83% field of community development and areas of development village 22.97%. In general management of the allocation of fu...
Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to a... more Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to available clinical treatments. The herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) system produces the selective elimination of HSVtk-positive cells and is a candidate for preclinical testing against glioblastoma via its ability to regulate proliferation and differentiation. Therefore, in this study, we aimed to establish a plasmid encoding the HSVtk/GCV system driven by a glial fibrillary acidic protein (GFAP) promoter and verify its possibility of neural differentiation of glioblastoma cell line under the GCV challenge. Four stable clones-N2A-pCMV-HSVtk, N2A-pGFAP-HSVtk, U251-pCMV-HSVtk, and U251-pGFAP-HSVtk-were established from neuronal N2A and glioblastoma U251 cell lines. In vitro GCV sensitivity was assessed by MTT assay for monitoring time-and dosage-dependent cytotoxicity. The capability for neural differentiation in stable glioblastoma clones during GCV treatment was assessed by performing immunocytochemistry for nestin, GFAP, and βIII-tubulin. Under GFAP promoter control, the U251 stable clone exhibited GCV sensitivity, while the neuronal N2A clones were nonreactive. During GCV treatment, cells underwent apoptosis on day 3 and dying cells were identified after day 5. Nestin was increasingly expressed in surviving cells, indicating that the population of neural stem-like cells was enriched. Lower levels of GFAP expression were detected in surviving cells. Furthermore, βIII-tubulin-positive neuron-like cells were identified after GCV treatment. This study established pGFAP-HSVtk-P2A-EGFP plasmids that successfully ablated GFAP-positive glioblastoma cells, but left neuronal N2A cells intact. These data suggest that the neural differentiation of glioblastoma cells can be promoted by treatment with the HSVtk/GCV system.
RELASI : JURNAL EKONOMI, 2021
Academic fraud can occur in university because it is influenced by various underlying factors, bo... more Academic fraud can occur in university because it is influenced by various underlying factors, both internally and externally. The purpose of holding this study was to determine whether the pressure, opportunity, rationalization, and abilities possessed by students significantly influence the occurrence of academic fraud in university. This research was conducted on 92 students of the Accounting Major in East Java "Veteran" National Development University by filling out a questionnaire via Google Form. The results of data collection were processed statistically using SmartPLS. The results of data analysis show that pressure, rationalization, and ability have a significant effect on academic fraud, while opportunities do not significantly influence students because lecturers always provide objective assessments to students, thereby reducing the chance of academic fraud. Keywords: Diamond Fraud Theory, Academic Fraud
JEA17: Jurnal Ekonomi Akuntansi, 2018
ABSTRACTThis study aims to describe how the management of the Village Fund Allocation and the Vil... more ABSTRACTThis study aims to describe how the management of the Village Fund Allocation and the Village Fund implemented in Tulungrejo districts Donomulyo Malang district, which is in the process of planning, implementation and accountability if it is based on procedures and rules, whether the principles princip management has been able to be realized. And supporting factors and inhibitors that affect the management of the Village Fund Allocation and the village fund. In this research, the calculation to determine the amount of the management of the funds used for the fields in rural development. The results of data analysis showed that the allocation of funds and funds rural village in the village which Tulungrejo in use for four areas of rural development is the empowerment of the community amounted to 81%, the field of administration of the village of 47.16%, 41.83% field of community development and areas of development village 22.97%. In general management of the allocation of fu...