Dmytro Snisarenko | Université Paul Sabatier de Toulouse (original) (raw)

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Papers by Dmytro Snisarenko

Research paper thumbnail of Clairance de molécules de taille moyenne à travers un rein artificiel

Despite a long history of development, the hemodialysis procedure (artificial kidney) still posse... more Despite a long history of development, the hemodialysis procedure (artificial kidney) still possesses some limitations, such as loss of the initial properties of the membrane due to fouling and poor removal of the middle sized uremic toxins. The present study is part of an European project named BioArt the aim of which was to overcome these limitations. In that objective, one of the partners of BioArt project reported on the development of the novel promising concept of double layer membrane with embedded adsorptive particles. A thorough characterization of the new membrane was then necessary, more precisely the extent to which mixed matrix layer can improve the removal of the uremic toxins from various groups needed to be evaluated, as well as the propensity of the membrane material to become fouled. The studies of the fouling phenomena are conventionally performed at the macro scale (bundle of hollow fibers) without insights of what is happening at the scale of an isolated fiber. ...

Research paper thumbnail of Medium sized molecules clearance through artificial kidneys

Despite a long history of development, the hemodialysis procedure (artificial kidney) still posse... more Despite a long history of development, the hemodialysis procedure (artificial kidney) still possesses some limitations, such as loss of the initial properties of the membrane due to fouling and poor removal of the middle sized uremic toxins. The present study is part of an European project named BioArt the aim of which was to overcome these limitations. In that objective, one of the partners of BioArt project reported on the development of the novel promising concept of double layer membrane with embedded adsorptive particles. A thorough characterization of the new membrane was then necessary, more precisely the extent to which mixed matrix layer can improve the removal of the uremic toxins from various groups needed to be evaluated, as well as the propensity of the membrane material to become fouled. The studies of the fouling phenomena are conventionally performed at the macro scale (bundle of hollow fibers) without insights of what is happening at the scale of an isolated fiber. ...

Research paper thumbnail of Insight into the transport mechanism of solute removed in dialysis by a membrane with double functionality

Chemical Engineering Research and Design, 2017

OATAO is an open access repository that collects the work of Toulouse researchers and makes it fr... more OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible.

Research paper thumbnail of Preparation and characterization of microfiltration flat polymeric membranes for biomedical applications

Preparation and characterization of microfiltration flat polymeric membranes for biomedical appli... more Preparation and characterization of microfiltration flat polymeric membranes for biomedical applications The EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities (http://www.em3e.eu). Copyright @ Name, FCT/UNL A Faculdade de Ciências e Tecnologia e a Universidade Nova de Lisboa têm o direito, perpétuo e sem limites geográficos, de arquivar e publicar esta dissertação através de exemplares impressos reproduzidos em papel ou de forma digital, ou por qualquer outro meio conhecido ou que venha a ser inventado, e de a divulgar através de repositórios científicos e de admitir a sua cópia e distribuição com objectivos educacionais ou de investigação, não comerciais, desde que seja dado crédito ao autor e editor. Projecto financiado com o apoio da Comissão Europeia. A informação contida nesta publicação vincula exclusivamente o autor, não sendo a Comissão responsável pela utilização que dela possa ser feita. I would like to express my gratitude to Mr. Luca Querze and Mr. Davide Rinaldi for the useful comments, remarks and engagement through the whole process of this master thesis preparation. Furthermore I would like to thank the services and help from Alessia Campisi working in HR department. Additionally I want to mention the contribution of Ing. Nino Gaeta in the organization of my stay in GVS Filter Technology. My deep appreciation also goes to all members of the membrane research group (Fausto Lentini, Felice Sidari, Elsa Caruso) for their advice and assistance. I would like to thank Weibing Feng for SEM analysis and permporometry tests. I would like to thank the examination committee for their advices and comments on my thesis. I would like to thank all my best friends for their support and encouragement. Finally, I would like to thank my family and parents for their love and support.

Research paper thumbnail of Combining fluorescence and permeability measurements in a membrane microfluidic device to study protein sorption mechanisms

Journal of Membrane Science, 2020

Membrane fouling by proteins is an important problem in hemodialysis or hemofiltration (artificia... more Membrane fouling by proteins is an important problem in hemodialysis or hemofiltration (artificial kidney). The mechanisms leading to fouling are still not fully understood and then predictable. In this paper we describe a microfluidic chip fitted with a filtration membrane which allows the real time in situ fluorescent analysis of labelled proteins and the measurement of the membrane permeability. The apparent kinetics rates of adsorption derived from the changes in fluorescence signal are combined with permeability measurements. This allows to discriminate two clearly distinct fouling mechanism by Bovine Serum Albumin (BSA) and α-lactalbumin (LALBA). The fouling kinetics of BSA is very rapid, independent of the flow conditions and can then be viewed as a protein monolayer adsorption controlled by protein-membrane interactions. In contrast, the fouling kinetics by LALBA is slower and very sensitive to flow conditions. We also describe a fluorescence quenching induced by protein aggregation and compression at high permeation rate. The fouling mechanism can then be viewed as a flow induced aggregation followed by a deposition of aggregates on the membrane. The complexity of sorption mechanisms on membrane during cross-flow filtration can be unraveled with this experimental setup .

Research paper thumbnail of Clairance de molécules de taille moyenne à travers un rein artificiel

Despite a long history of development, the hemodialysis procedure (artificial kidney) still posse... more Despite a long history of development, the hemodialysis procedure (artificial kidney) still possesses some limitations, such as loss of the initial properties of the membrane due to fouling and poor removal of the middle sized uremic toxins. The present study is part of an European project named BioArt the aim of which was to overcome these limitations. In that objective, one of the partners of BioArt project reported on the development of the novel promising concept of double layer membrane with embedded adsorptive particles. A thorough characterization of the new membrane was then necessary, more precisely the extent to which mixed matrix layer can improve the removal of the uremic toxins from various groups needed to be evaluated, as well as the propensity of the membrane material to become fouled. The studies of the fouling phenomena are conventionally performed at the macro scale (bundle of hollow fibers) without insights of what is happening at the scale of an isolated fiber. ...

Research paper thumbnail of Medium sized molecules clearance through artificial kidneys

Despite a long history of development, the hemodialysis procedure (artificial kidney) still posse... more Despite a long history of development, the hemodialysis procedure (artificial kidney) still possesses some limitations, such as loss of the initial properties of the membrane due to fouling and poor removal of the middle sized uremic toxins. The present study is part of an European project named BioArt the aim of which was to overcome these limitations. In that objective, one of the partners of BioArt project reported on the development of the novel promising concept of double layer membrane with embedded adsorptive particles. A thorough characterization of the new membrane was then necessary, more precisely the extent to which mixed matrix layer can improve the removal of the uremic toxins from various groups needed to be evaluated, as well as the propensity of the membrane material to become fouled. The studies of the fouling phenomena are conventionally performed at the macro scale (bundle of hollow fibers) without insights of what is happening at the scale of an isolated fiber. ...

Research paper thumbnail of Insight into the transport mechanism of solute removed in dialysis by a membrane with double functionality

Chemical Engineering Research and Design, 2017

OATAO is an open access repository that collects the work of Toulouse researchers and makes it fr... more OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible.

Research paper thumbnail of Preparation and characterization of microfiltration flat polymeric membranes for biomedical applications

Preparation and characterization of microfiltration flat polymeric membranes for biomedical appli... more Preparation and characterization of microfiltration flat polymeric membranes for biomedical applications The EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities (http://www.em3e.eu). Copyright @ Name, FCT/UNL A Faculdade de Ciências e Tecnologia e a Universidade Nova de Lisboa têm o direito, perpétuo e sem limites geográficos, de arquivar e publicar esta dissertação através de exemplares impressos reproduzidos em papel ou de forma digital, ou por qualquer outro meio conhecido ou que venha a ser inventado, e de a divulgar através de repositórios científicos e de admitir a sua cópia e distribuição com objectivos educacionais ou de investigação, não comerciais, desde que seja dado crédito ao autor e editor. Projecto financiado com o apoio da Comissão Europeia. A informação contida nesta publicação vincula exclusivamente o autor, não sendo a Comissão responsável pela utilização que dela possa ser feita. I would like to express my gratitude to Mr. Luca Querze and Mr. Davide Rinaldi for the useful comments, remarks and engagement through the whole process of this master thesis preparation. Furthermore I would like to thank the services and help from Alessia Campisi working in HR department. Additionally I want to mention the contribution of Ing. Nino Gaeta in the organization of my stay in GVS Filter Technology. My deep appreciation also goes to all members of the membrane research group (Fausto Lentini, Felice Sidari, Elsa Caruso) for their advice and assistance. I would like to thank Weibing Feng for SEM analysis and permporometry tests. I would like to thank the examination committee for their advices and comments on my thesis. I would like to thank all my best friends for their support and encouragement. Finally, I would like to thank my family and parents for their love and support.

Research paper thumbnail of Combining fluorescence and permeability measurements in a membrane microfluidic device to study protein sorption mechanisms

Journal of Membrane Science, 2020

Membrane fouling by proteins is an important problem in hemodialysis or hemofiltration (artificia... more Membrane fouling by proteins is an important problem in hemodialysis or hemofiltration (artificial kidney). The mechanisms leading to fouling are still not fully understood and then predictable. In this paper we describe a microfluidic chip fitted with a filtration membrane which allows the real time in situ fluorescent analysis of labelled proteins and the measurement of the membrane permeability. The apparent kinetics rates of adsorption derived from the changes in fluorescence signal are combined with permeability measurements. This allows to discriminate two clearly distinct fouling mechanism by Bovine Serum Albumin (BSA) and α-lactalbumin (LALBA). The fouling kinetics of BSA is very rapid, independent of the flow conditions and can then be viewed as a protein monolayer adsorption controlled by protein-membrane interactions. In contrast, the fouling kinetics by LALBA is slower and very sensitive to flow conditions. We also describe a fluorescence quenching induced by protein aggregation and compression at high permeation rate. The fouling mechanism can then be viewed as a flow induced aggregation followed by a deposition of aggregates on the membrane. The complexity of sorption mechanisms on membrane during cross-flow filtration can be unraveled with this experimental setup .