Joseba koldo | Universitat Politècnica de València (original) (raw)
Uploads
Papers by Joseba koldo
Journal of Food Science and Technology, 2018
The b-galactosidase is an industrially valuable enzyme and used to hydrolyze the lactose into glu... more The b-galactosidase is an industrially valuable enzyme and used to hydrolyze the lactose into glucose and galactose. Considering the broad utility profile in food industry, b-galactosidase from Aspergillus nidulans was purified and characterized in term of its catalytic properties and stability. It displayed highest catalytic efficiency at 60°C after 10.0 min within acidic pH environment (pH 5). The b-galactosidase exhibited 100% and 60% catalytic activity at 40°C and 50°C, respectively even after 120.0 min. The b-galactosidase activity was remained stable in the presence of Zn 2? , Ni 2? , and Mg 2? ions. The activity was also retained in all investigated organic solvents except DMSO at various ionic concentrations. The surfactants Triton X-100 and SDS caused positive impact on the catalytic activity of enzyme at 1.0 mM concentration. However, the percent relative activity of b-galactosidase was significantly reduced when incubated with EDTA. The molecular mass of b-galactosidase estimated to be 95 kDa. The SEM micrographs of ONPG before and after b-galactosidase treatment indicated a remarkable difference in the morphology and proved the strong catalytic strength of enzyme. The b-galactosidase also demonstrated exceptional storage stability at-80°C,-20°C and 4°C by retaining 86, 79 and 70% activity even after 100.0 days.
Journal of Food Science and Technology, 2018
The b-galactosidase is an industrially valuable enzyme and used to hydrolyze the lactose into glu... more The b-galactosidase is an industrially valuable enzyme and used to hydrolyze the lactose into glucose and galactose. Considering the broad utility profile in food industry, b-galactosidase from Aspergillus nidulans was purified and characterized in term of its catalytic properties and stability. It displayed highest catalytic efficiency at 60°C after 10.0 min within acidic pH environment (pH 5). The b-galactosidase exhibited 100% and 60% catalytic activity at 40°C and 50°C, respectively even after 120.0 min. The b-galactosidase activity was remained stable in the presence of Zn 2? , Ni 2? , and Mg 2? ions. The activity was also retained in all investigated organic solvents except DMSO at various ionic concentrations. The surfactants Triton X-100 and SDS caused positive impact on the catalytic activity of enzyme at 1.0 mM concentration. However, the percent relative activity of b-galactosidase was significantly reduced when incubated with EDTA. The molecular mass of b-galactosidase estimated to be 95 kDa. The SEM micrographs of ONPG before and after b-galactosidase treatment indicated a remarkable difference in the morphology and proved the strong catalytic strength of enzyme. The b-galactosidase also demonstrated exceptional storage stability at-80°C,-20°C and 4°C by retaining 86, 79 and 70% activity even after 100.0 days.