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Papers by Juan Serrano Viseas

Research paper thumbnail of Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays

Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies, 2018

This paper presents the fitting process followed to adjust the parameters of the electrical model... more This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture assays. A new parameter matching procedure is proposed, under the basis of both, mismatching between electrodes and time-evolution observed in the system response, as consequence of electrode fabrication processes and electrochemical performance of electrode-solution interface, respectively. The obtained results agree with experimental performance, and enable the evaluation of the cell number in a culture, by using the electrical measurements observed at the oscillation parameters in the test circuits employed.

Research paper thumbnail of Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies, 2021

This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) ce... more This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct Current (DC) and Alternated Current (AC) time dependent electric field protocols were applied to N2A cell culture under differentiation conditions, obtaining different responses. Control and electrically excited samples' number of differentiated cells and neurite lengths were measure after differentiation. Results showed that DC fields have a strong influence on N2A differentiation since the percentage of differentiated cells and the neurites lengths were the highest. In addition, a significant alignment of neurites measured with the applied electrical field has been detected, which demonstrates the high sensitivity of differentiation processes to electrical field polarity.

Research paper thumbnail of Remote Cell Growth Sensing Using Self-Sustained Bio-Oscillations

Sensors (Basel, Switzerland), Jan 3, 2018

A smart sensor system for cell culture real-time supervision is proposed, allowing for a signific... more A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable "biological" oscillator. The system enables the remote acquisition and management of the "biological" oscillation signals through a secure web interface. The indirectly observed biological properties are cell growth and cell number, which are straightforwardly related to the measured bio-oscillation signal parameters, i.e., frequency and amplitude. The sensor extracts the information without complex circuitry for acquisition and measurement, taking advantage of the microcontroller features. A discrete prototype for sensing and remote monitoring is presented along with the experimental results obtained from the performed measurements, achieving the expected performance and outcomes.

Research paper thumbnail of Control predictivo aplicado al modelo macroscópico del tráfico en una ciudad

El trabajo consiste en la estimación de un modelo macroscópico del comportamiento del tráfico urb... more El trabajo consiste en la estimación de un modelo macroscópico del comportamiento del tráfico urbano (en especial de vehiculos eléctricos), y en la implementación en simulación de un control predictivo que maximice los beneficios de las estaciones de recarga.Universidad de Sevilla. Máster en Automática, Robótica y Telemátic

Research paper thumbnail of An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

Sensors (Basel, Switzerland), Jan 20, 2018

This paper proposes a new yet efficient method allowing a significant improvement in the on-line ... more This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be extrapolated for any type of cellular line used in electrical cell-substrate impedance spectroscopy (ECIS) tests. Parameters of the bioimpedance model, acquired from ECIS experiments, vary for each cell line, which makes obtaining results difficult and-to some extent-renders them inaccurate. We propose a fitting method based on the cell line initial characterization, and carry out subsequent experiments with the same line to approach the percentage of well filling and the cell density (or cell number in the well). To perform our calibration technique, the so-called oscillation-based test (OBT) approach is employed for each cell density. Calibration results are validated by performing othe...

Research paper thumbnail of Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays

Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies, 2018

This paper presents the fitting process followed to adjust the parameters of the electrical model... more This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture assays. A new parameter matching procedure is proposed, under the basis of both, mismatching between electrodes and time-evolution observed in the system response, as consequence of electrode fabrication processes and electrochemical performance of electrode-solution interface, respectively. The obtained results agree with experimental performance, and enable the evaluation of the cell number in a culture, by using the electrical measurements observed at the oscillation parameters in the test circuits employed.

Research paper thumbnail of Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies, 2021

This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) ce... more This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct Current (DC) and Alternated Current (AC) time dependent electric field protocols were applied to N2A cell culture under differentiation conditions, obtaining different responses. Control and electrically excited samples' number of differentiated cells and neurite lengths were measure after differentiation. Results showed that DC fields have a strong influence on N2A differentiation since the percentage of differentiated cells and the neurites lengths were the highest. In addition, a significant alignment of neurites measured with the applied electrical field has been detected, which demonstrates the high sensitivity of differentiation processes to electrical field polarity.

Research paper thumbnail of Remote Cell Growth Sensing Using Self-Sustained Bio-Oscillations

Sensors (Basel, Switzerland), Jan 3, 2018

A smart sensor system for cell culture real-time supervision is proposed, allowing for a signific... more A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable "biological" oscillator. The system enables the remote acquisition and management of the "biological" oscillation signals through a secure web interface. The indirectly observed biological properties are cell growth and cell number, which are straightforwardly related to the measured bio-oscillation signal parameters, i.e., frequency and amplitude. The sensor extracts the information without complex circuitry for acquisition and measurement, taking advantage of the microcontroller features. A discrete prototype for sensing and remote monitoring is presented along with the experimental results obtained from the performed measurements, achieving the expected performance and outcomes.

Research paper thumbnail of Control predictivo aplicado al modelo macroscópico del tráfico en una ciudad

El trabajo consiste en la estimación de un modelo macroscópico del comportamiento del tráfico urb... more El trabajo consiste en la estimación de un modelo macroscópico del comportamiento del tráfico urbano (en especial de vehiculos eléctricos), y en la implementación en simulación de un control predictivo que maximice los beneficios de las estaciones de recarga.Universidad de Sevilla. Máster en Automática, Robótica y Telemátic

Research paper thumbnail of An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

Sensors (Basel, Switzerland), Jan 20, 2018

This paper proposes a new yet efficient method allowing a significant improvement in the on-line ... more This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be extrapolated for any type of cellular line used in electrical cell-substrate impedance spectroscopy (ECIS) tests. Parameters of the bioimpedance model, acquired from ECIS experiments, vary for each cell line, which makes obtaining results difficult and-to some extent-renders them inaccurate. We propose a fitting method based on the cell line initial characterization, and carry out subsequent experiments with the same line to approach the percentage of well filling and the cell density (or cell number in the well). To perform our calibration technique, the so-called oscillation-based test (OBT) approach is employed for each cell density. Calibration results are validated by performing othe...