Calin Mircu | University of Life Sciences "King Michael I" from Timisoara (original) (raw)
Papers by Calin Mircu
Animals
Congenital abnormalities in animals, including abnormalities of the cleft lip and jaw and hypospa... more Congenital abnormalities in animals, including abnormalities of the cleft lip and jaw and hypospadias have been reported in all domesticated species. They are a major concern for breeders due to the increased economic loss they entail. In this article, we described a congenital bilateral cheilognathoschisis (cleft lip and jaw) with campylognathia in association with penile hypospadias and preputial hypoplasia with failure of preputial fusion in a Bos taurus crossbred Piedmontese × Wagyu calf. Clinical examination, computed tomography, and whole genome sequencing were performed to describe and identify a possible cause of the abnormalities. Clinical examination revealed a bilateral cheilognathoschisis of approximately 4 cm in length and 3 cm in width in the widest part, with computer tomography analyses confirming the bilateral absence of the processus nasalis of the incisive bone and the lateral deviation of the processus palatinus towards the left side. Genomic data analyses identi...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
The resistance to antimicrobial substances severely impacts public health and the abuse of antibi... more The resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic "resistome" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including Streptococcus spp., Staphylococcus spp. and Escherichia coli. AMR gene expression testing in bacteria involved in mastitis in dairy cows was performed. Milk samples were subjected to the California Mastitis Test. Positive samples were transferred using eSwab, cultured on Columbia blood agar and on MacConkey agar. The Qiagen DNeasy kit was used for DNA extraction and qPCRs were run using an Agilent thermocycler. In most of the samples tested (n = 42, from three different lactating farms), the presence of ampC (36 out of 42; 85.7%) and blaZ (95.2%), correlated with confirmed resistance to beta-lactam and cephalosporin antibiotics. A variable presence of other tested AMR genes was detected,...
Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduct... more Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduction, studying are done also on animal oocyte cryopreservation in order to find the best conditions to preserve their viability. Vitrification is one of the methods that can be used in order to preserve oocytes. The higher reactive oxygen species that are formed during in vitro conditions can influence the success of assisted reproduction technique. The aim of this study was to evaluate the antioxidant potential of ascorbic acid (0.5mM) and rosmarinic acid (105µM) added in media for in vitro maturation on swine oocyte subjected to vitrification. COC's viability after vitrification was evaluated by 0.02% Trypan Blue staining. Comparing experimental groups C (vitamin C) and AR (rosmarinic acid) with group M (control), relative to the number of vitrified oocytes, a slight increase in their viability is observed, with 16.67% (C, class I) and 33.33% (AR, class II), respectively. Regardless of the treatment applied, the oocyte class is associated with viability (p = 0.048). Due to low number of oocytes used in each group we can concluded that supplementation of oocyte maturation media before vitrification with rosmarinic acid and ascorbic acid could produce a slight increase in viability.
The study was carried on detection of mastitis, using increasing respectively decreasing trend of... more The study was carried on detection of mastitis, using increasing respectively decreasing trend of somatic cells count (SCC) of two successive samplings. The analysis was performed to identify the associations between SCC trend with retrospective and transversal milk quality indicators, and mastitis. From a BIOAMR database, 28 cows with a posteriori diagnosed mastitis with Staphylococcus aureus (4/28 cases were methicillin-resistant Staphylococcus aureus-MRSA) were sampled. The trend of SCC was Spearman's rho correlated with previous lactose (rs = +0.785, p=0.03), pH (rs = +0.662 at p=0.019), and current SCC measurements (rs = +0.781, at p=0.
Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects ... more Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique. The objective of the study was to determine the effect of rosmarinic acid addition to the in vitro culture media on bovine oocytes maturation rate based on morphological changes. Bovine COC's were matured according to their morphological class (class I, II and III) in two groups: control (M) and supplemented with rosmarinic acid (105 µM, AR) in TCM 199 HEPES modification media at 38.5 0 C in 5% CO2 humidified air atmosphere for 24h. Comparing the groups, relative to the number of COC's matured, a increase in their maturation features is observed, with 26.81% % (AR1), 21.67% (AR2) and 23.34% (AR3), respectively in groups supplemented with rosmarinic acid. The oocyte class is associated with their capacity to develop in vitro based on their morphological examination.
Iron is essential elements for human, animals and plant organism. The depletion of organism in ir... more Iron is essential elements for human, animals and plant organism. The depletion of organism in iron leads to modification in the concentration of hemoglobin which is responsible for oxygen transportation. Also, overload of iron represents a possible intoxication that can affect negatively the liver tissue and then all cells. Our experiments was conducted on two groups (control and experimental) of rabbits-German Lop Eared Rabbits breed, and we administrated ferrous gluconate (10mg/kg body weight) to the experimental group, two times. Also, during the experiment the rabbits had a special diet with plants from garden administrated fresh. The plants have very good antioxidant properties, and can prevent the iron intoxication and also participate intense to detoxification of the liver after iron overload. Hematological analysis were preformed for red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width, platelet and leukocyte evaluation. The results demonstrated a very good protective action of the diet plants, meaning that the hematological parameters were not out of normal range after iron overload.
Animals
Congenital abnormalities in animals, including abnormalities of the cleft lip and jaw and hypospa... more Congenital abnormalities in animals, including abnormalities of the cleft lip and jaw and hypospadias have been reported in all domesticated species. They are a major concern for breeders due to the increased economic loss they entail. In this article, we described a congenital bilateral cheilognathoschisis (cleft lip and jaw) with campylognathia in association with penile hypospadias and preputial hypoplasia with failure of preputial fusion in a Bos taurus crossbred Piedmontese × Wagyu calf. Clinical examination, computed tomography, and whole genome sequencing were performed to describe and identify a possible cause of the abnormalities. Clinical examination revealed a bilateral cheilognathoschisis of approximately 4 cm in length and 3 cm in width in the widest part, with computer tomography analyses confirming the bilateral absence of the processus nasalis of the incisive bone and the lateral deviation of the processus palatinus towards the left side. Genomic data analyses identi...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
The resistance to antimicrobial substances severely impacts public health and the abuse of antibi... more The resistance to antimicrobial substances severely impacts public health and the abuse of antibiotics leads to antimicrobial resistance (AMR) or the antibiotic "resistome" (Wright, 2007). Bovine mastitis is largely diagnosed in dairy farms and is caused by a variety of pathogens including Streptococcus spp., Staphylococcus spp. and Escherichia coli. AMR gene expression testing in bacteria involved in mastitis in dairy cows was performed. Milk samples were subjected to the California Mastitis Test. Positive samples were transferred using eSwab, cultured on Columbia blood agar and on MacConkey agar. The Qiagen DNeasy kit was used for DNA extraction and qPCRs were run using an Agilent thermocycler. In most of the samples tested (n = 42, from three different lactating farms), the presence of ampC (36 out of 42; 85.7%) and blaZ (95.2%), correlated with confirmed resistance to beta-lactam and cephalosporin antibiotics. A variable presence of other tested AMR genes was detected,...
Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduct... more Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduction, studying are done also on animal oocyte cryopreservation in order to find the best conditions to preserve their viability. Vitrification is one of the methods that can be used in order to preserve oocytes. The higher reactive oxygen species that are formed during in vitro conditions can influence the success of assisted reproduction technique. The aim of this study was to evaluate the antioxidant potential of ascorbic acid (0.5mM) and rosmarinic acid (105µM) added in media for in vitro maturation on swine oocyte subjected to vitrification. COC's viability after vitrification was evaluated by 0.02% Trypan Blue staining. Comparing experimental groups C (vitamin C) and AR (rosmarinic acid) with group M (control), relative to the number of vitrified oocytes, a slight increase in their viability is observed, with 16.67% (C, class I) and 33.33% (AR, class II), respectively. Regardless of the treatment applied, the oocyte class is associated with viability (p = 0.048). Due to low number of oocytes used in each group we can concluded that supplementation of oocyte maturation media before vitrification with rosmarinic acid and ascorbic acid could produce a slight increase in viability.
The study was carried on detection of mastitis, using increasing respectively decreasing trend of... more The study was carried on detection of mastitis, using increasing respectively decreasing trend of somatic cells count (SCC) of two successive samplings. The analysis was performed to identify the associations between SCC trend with retrospective and transversal milk quality indicators, and mastitis. From a BIOAMR database, 28 cows with a posteriori diagnosed mastitis with Staphylococcus aureus (4/28 cases were methicillin-resistant Staphylococcus aureus-MRSA) were sampled. The trend of SCC was Spearman's rho correlated with previous lactose (rs = +0.785, p=0.03), pH (rs = +0.662 at p=0.019), and current SCC measurements (rs = +0.781, at p=0.
Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects ... more Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique. The objective of the study was to determine the effect of rosmarinic acid addition to the in vitro culture media on bovine oocytes maturation rate based on morphological changes. Bovine COC's were matured according to their morphological class (class I, II and III) in two groups: control (M) and supplemented with rosmarinic acid (105 µM, AR) in TCM 199 HEPES modification media at 38.5 0 C in 5% CO2 humidified air atmosphere for 24h. Comparing the groups, relative to the number of COC's matured, a increase in their maturation features is observed, with 26.81% % (AR1), 21.67% (AR2) and 23.34% (AR3), respectively in groups supplemented with rosmarinic acid. The oocyte class is associated with their capacity to develop in vitro based on their morphological examination.
Iron is essential elements for human, animals and plant organism. The depletion of organism in ir... more Iron is essential elements for human, animals and plant organism. The depletion of organism in iron leads to modification in the concentration of hemoglobin which is responsible for oxygen transportation. Also, overload of iron represents a possible intoxication that can affect negatively the liver tissue and then all cells. Our experiments was conducted on two groups (control and experimental) of rabbits-German Lop Eared Rabbits breed, and we administrated ferrous gluconate (10mg/kg body weight) to the experimental group, two times. Also, during the experiment the rabbits had a special diet with plants from garden administrated fresh. The plants have very good antioxidant properties, and can prevent the iron intoxication and also participate intense to detoxification of the liver after iron overload. Hematological analysis were preformed for red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width, platelet and leukocyte evaluation. The results demonstrated a very good protective action of the diet plants, meaning that the hematological parameters were not out of normal range after iron overload.