Jesus Romalde | University of Santiago de Compostela (original) (raw)

Papers by Jesus Romalde

PLOS ONE, Aug 15, 2023

A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated str... more A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated strain PT1 T , was isolated from the laboratory-reared larvae of the sea cucumber Apostichopus japonicus. A phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT1 T was closely related to Neptuniibacter marinus ATR 1.1 T (= CECT 8938 T = DSM 100783 T) and Neptuniibacter caesariensis MED92 T (= CECT 7075 T = CCUG 52065 T) showing 98.2% and 98.1% sequence similarity, respectively. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were 72.0%-74.8% and 18.3%-19.5% among related Neptuniibacter species, which were below 95% and 70%, respectively, confirming the novel status of PT1 T. The average amino acid identity (AAI) values of PT1 T showing 74-77% among those strains indicated PT1 T is a new species in the genus Neptuniibacter. Based on the genome-based taxonomic approach, Neptuniibacter victor sp. nov.

Current Microbiology, Dec 14, 2021

 Consent to participate (Not applicable)  Consent for publication (Not applicable)  Availabili... more  Consent to participate (Not applicable)  Consent for publication (Not applicable)  Availability of data and material The whole genome sequence data obtained in this study was deposited at DDBJ/EMBL/GenBank under BioProject Accession: PRJDB11924.  Code availability (Not applicable) Author Contributions  Chunqi Jiang conceived, designed and performed the experiments, analyzed the data, visualized the data, drafted and reviewed the manuscript.  Mami Tanaka and Sayo Nishikawa performed the experiments, reviewed the manuscript.

Applied and Environmental Microbiology, Aug 1, 1994

We evaluated the iron uptake mechanisms in Pasteurella piscicida strains as well as the effect of... more We evaluated the iron uptake mechanisms in Pasteurella piscicida strains as well as the effect of iron overload on the virulence of these strains for fish. With this aim, the capacity of the strains to obtain iron from transferrin and heme compounds as well as their ability to overcome the inhibitory activity of fish serum was analyzed. All the P. piscicida strains grew in the presence of the iron chelator ethylene-diamine-di (0hydroxyphenyl acetic acid) or of human transferrin, which was used by a siderophore-mediated mechanism. The chemical tests and cross-feeding assays showed that P. piscicida produced a siderophore which was neither a phenolate nor a hydroxamate. Cross-feeding assays as well as preliminary chromatographic analysis suggest that this siderophore may be chemically related to multocidin. All the P. piscicida isolates utilized hemin and hemoglobin as an iron source, since the virulence of the strains increased when the fish were preinoculated with these compounds. This effect was stronger in the avirulent strains (50% lethal dose was reduced by 4 logs when fish were pretreated with hemin or hemoglobin). Only the pathogenic P. piscicida isolates were resistant to the bactericidal action of the fresh fish serum. The nonpathogenic strains grew in fish serum only when it was heat-inactivated or when it was supplemented with ferric ammonium citrate, hemin, or hemoglobin. In all the strains, at least three iron-regulated outer membrane proteins (IROMPs) (105, 118, and 145 kDa) were increased when the strains were cultured in iron-restricted medium. In addition, although a new IROMP (75 kDa) was expressed in some isolates, this protein was not associated with the virulence of the strains. All the IROMPs were immunologically related in all the P. piscicida isolates. It is noteworthy that iron also played a regulatory role in the synthesis of some proteolytic enzymes, since these activities were expressed only when the strains were cultured in iron-depleted conditions.

Diseases of Aquatic Organisms, 2002

PLOS ONE

The genus Thalassotalea is ubiquitous in marine environments, and up to 20 species have been desc... more The genus Thalassotalea is ubiquitous in marine environments, and up to 20 species have been described so far. A Gram-staining-negative, aerobic bacterium, designated strain PTE2T was isolated from laboratory-reared larvae of the Japanese sea cucumber Apostichopus japonicus. Phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PTE2T was closely related to Thalassotalea sediminis N211T (= KCTC 42588T = MCCC 1H00116T) with 97.9% sequence similarity. ANI and in silico DDH values against Thalassotalea species were 68.5–77.0% and 19.7–24.6%, respectively, indicating the novelty of PTE2T. Based on genome-based taxonomic approaches, strain PTE2T (= JCM 34608T = KCTC 82592T) is proposed as a new species, Thalassotalea hakodatensis sp. nov.

Con este articulo se pretende exponer de forma concisa los principales problemas patologicos, bac... more Con este articulo se pretende exponer de forma concisa los principales problemas patologicos, bacterianos y viricos, que afectan hoy en dia al cultivo de las especies de peces mas importantes, desde el punto de vista economico, en nuestro pais, haciendo especial hincapie en la efectividad de las medidas de control y prevencion empleadas normalmente en las plantas de cultivo. La informacion presentada en el mismo tiene un caracter divulgativo y esta basada en los resultados de las investigaciones llevadas a cabo en nuestro Departamento durante los ultimos anos.

Reviews: Methods and Technologies in Fish Biology and Fisheries, 2002

Streptococcosis (also known as streptococcicosis) is an emerging pathology affecting a variety of... more Streptococcosis (also known as streptococcicosis) is an emerging pathology affecting a variety of wild and cultured fish all over the world. It constitutes one of the most threatening diseases of cultured rainbow trout in different geographical areas such as Europe, Japan and U.S.A. The main objective of this review is to analyze the current state of knowledge regarding new diagnostic procedures for this important disease. In addition, we describe the different Gram-positive bacteria implicated with this syndrome in order to gain some perspective of the complex etiology of this infection.

Pathogens, Parasites and Predators, 2009

Pathology in Marine Science, 1990

ABSTRACT: Galicia (Northwestern Spain) is a very important environment for marine aquaculture. Ho... more ABSTRACT: Galicia (Northwestern Spain) is a very important environment for marine aquaculture. However, vibriosis caused by V. anguillarum and V. tubiashii is the most threatening bacterial disease limiting the production of marine fish and shellfish in our area (Toranzo et al., 1987; Lodeiros et al., 1987). The present work was carried out to establish the taxonomic, serological, and ecological relationship among isolates from diseased fish and oysters with similar estuarine vibrios.

Aquaculture, 2006

In this work, we evaluated the effect of the inclusion of different adjuvants in the vaccine form... more In this work, we evaluated the effect of the inclusion of different adjuvants in the vaccine formulation against Lactococcus garvieae in comparison with an aqueous bacterin. The aqueous and non mineral oil adjuvanted vaccines (Montanide-ISA-763-A and Aquamun) yielded a good protection 4 weeks after the immunization (RPS of 82.6, 84.8 and 86.9, respectively). The protective rates obtained for the adjuvants Montanide-IMS-2212 (water based nanoparticles with immunostimulant) and Carbomer (high molecular weight polymer organic of polyacrylic acid) were 45.7% and 56.5%, respectively. We also evaluated the duration of protection confered by Aquamun adjuvanted-vaccine in comparison with the aqueous bacterin. Three months after vaccination, the RPS values obtained with the aqueous vaccine was 40% whereas with the Aquamun adjuvanted-vaccine the protection increased to values of 92%. In addition, a long lasting protection was observed with this adjuvanted vaccine with RPS values of 90% and 83.3% in the challenges performed at 6 and 8 months post-vaccination, respectively.

Bulletin- European Association of Fish Pathologists

Two episodes of flexibacteriosis caused by T. maritimum were diagnosed in wild turbot and farmed ... more Two episodes of flexibacteriosis caused by T. maritimum were diagnosed in wild turbot and farmed tub gurnard in Italy. The two different cases occurred in the facilities of Universtiy of Camerino during reproduction trials. A group of 10 adult turbot showed haemorrhagic, frayed fins and eroded mouth two weeks after capture from the North Adriatic Sea. The disease was controlled by antibiotic therapy. A group of 20 tub gurnard showed eroded mouth, rotten fins and severe skin ulcerative lesions, after 3 days of transportations from a fish farm to our laboratory facilities. Mortality reached 90% of the population within 9 days. Traditional bacteriological and PCR analysis of the isolates in the two episodes allowed the identification of the causative agent as Tenacibaculum maritimum. Serological characterization of the isolates demonstrated that they belonged to serotype O3. Histopathological examination showed severe skin necrosis, dermis congestion associated with heterophilic and ma...

Journal of Fish Diseases

Yersinia ruckeri causes important economic losses for rainbow trout (Oncorhynchus mykiss) farms w... more Yersinia ruckeri causes important economic losses for rainbow trout (Oncorhynchus mykiss) farms worldwide. This bacterial disease is likely the most common among trout in Peru; however, no commercial vaccine is available nationally, which is, in part, due to a lack of information on the bacterium. The aim of the current study was to characterize 29 Y. ruckeri isolates sampled from seven cage‐reared farms in the Puno Region, the focal point for aquaculture activities in Peru. For this, samples were taken from fish with clinical signs (i.e. haemorrhages, uni‐ or bilateral exophthalmia, hyphaemia and/or melanosis). Notable among our findings was the existence of both Y. ruckeri biotype 1 (9 isolates) and biotype 2 (20 isolates; negative for sorbitol and Tween 80). The isolates further differed in API profiles 5307100 (21 isolates), 1307100 (4 isolates), 1305100 (2 isolates), 1307120 (1 isolate) and 5305100 (1 isolate), with the main differences being in the tests for lysine decarboxyla...

Bull. Eur. Assoc. Fish …, 2005

Tenacibaculum maritimum was consistently isolated from tenacibaculosis outbreaks affecting sole f... more Tenacibaculum maritimum was consistently isolated from tenacibaculosis outbreaks affecting sole farms in Portugal and south of Spain in the last two years. These new T. maritimum isolates could not be assigned within the two major serotypes (O1 and O2) already described. Rabbit antiserum was prepared against one Portuguese sole isolate to examine the antigenic relationships between the isolates from sole using microtitre agglutination tests, dot blot assay and immunoblotting of lipopolysaccharides. Serological characterization of the recent sole isolates demonstrated that they belong to a novel O-serotype named O3, allowing us to extend the serological scheme for this fish pathogen. This information is useful for epizootiological and vaccination studies.

Veterinary Microbiology, 2015

The present study deals with the first isolation of Vibrio toranzoniae from cultured red conger e... more The present study deals with the first isolation of Vibrio toranzoniae from cultured red conger eel (Genypterus chilensis). During the summer season of 2011, mortalities were observed in young red conger eel at one aquaculture experimental rearing system in Quintay, Valparaiso, Chile. The microbiological analysis of the diseased fish resulted in the isolation of three dominant and representative isolates, designated as R.17, R.18 and R.19, which were obtained from gill, fin and external lesions from three different fish, respectively. All isolates were identified as V. toranzoniae by means of a polyphasic taxonomic approach, including phenotypic characterization, sequencing of 16S rRNA and housekeeping genes, and DNA-DNA hybridization. Inoculation of a representative strain (R18) in turbot as model fish species demonstrated the pathogenic potential for fish of the Chilean isolates. Results obtained indicate that the geographical and host distribution of V. toranzoniae is wider than expected, and that this species may have negative incidence in the culture of marine organisms. 2015 Elsevier B.V. All rights reserved.

bioRxiv (Cold Spring Harbor Laboratory), Nov 12, 2020

Integrated and quantitative observations of antibiotic resistance genes (ARGs) in urban water sys... more Integrated and quantitative observations of antibiotic resistance genes (ARGs) in urban water systems (UWSs) are lacking. We sampled three UWSs for clinically important extended spectrum β-lactamase (ESBL) and carbapenemase (CP) genes, mobile genetic elements and microbial communities. Sewage-especially from hospitals-carried substantial loads of ESBL and CP genes (10 6-10 7 per person equivalent), but those loads progressively declined along the UWS, resulting in minimal emissions (10 1-10 4 copies per person equivalent). Removal was primarily during sewage conveyance (65% ± 36%) rather than within sewage treatment (34% ± 23%). The ARGs clustered in groups based on their persistence; less persistent groups were associated to putative host taxa (especially Enterobacteriaceae and Moraxellaceae), while more persistent groups appear horizontally transferred as they correlated with mobile genetic elements. This first documentation of a substantial ARG reduction during sewage conveyance provides opportunities for antibiotic resistance management and a caution for sewage-based ARG surveillance. .

European Journal of Histochemistry, Aug 17, 2009

Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.... more Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7x10 6 CFU/fish) but no to bath exposure. The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen.

Systematic and Applied Microbiology, Dec 1, 2016

Vibrio barjaei sp. nov., a new species of the Mediterranei clade isolated in a shellfish hatchery.

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