María Elena López Martín | University of Santiago de Compostela (original) (raw)

Papers by María Elena López Martín

IEEE Access

Programmed cell death constitutes a fundamental part of the immune response to viral infection. T... more Programmed cell death constitutes a fundamental part of the immune response to viral infection. This process forms part of the host defence mechanism and also enables establishment of biomarkers of disease severity. Natural or anthropogenic sources of microwaves emit energy and may alter the ecology of the SAR-CoV-2 virus (which causes COVID-19 disease) in the environment. Determining the associated effects on the immune system and on the health of hosts with COVID-19 disease is thus important. In this review paper, we consider studies analyzing the influence of electromagnetic fields on innate and acquired immune responses in humans, and above all on preclinical experimental animal models and in vitro models, and we also consider studies analyzing immunity acquired from COVID-19 infections associated with cell death. We focus on the effects of electromagnetic fields and the influence of oxidative stress on stimulation or immunomodulation, the inflammatory response, autoimmunity and the participation of intracellular calcium channels in the immunology of COVID-19 disease. Non-ionizing radiation can activate or reduce the inflammatory response, oxidative stress and the entry of intracellular calcium and can facilitate or reduce cell death. The review of experimental study findings indicates that exposure to non-ionizing radiation can also have a bidirectional effect on the immune system, either slowing down or enhancing the processes that lead to the cell death associated with COVID-19 disease. INDEX TERMS Cell death, COVID-19, immune cells, electromagnetic fields, non-ionizing radiation.

Scientific Reports

Difference far-field patterns represent a way for pin-pointing a target in both azimuth and eleva... more Difference far-field patterns represent a way for pin-pointing a target in both azimuth and elevation, extremely useful in radar applications. At the present work, an innovative method for synthesizing good compromise solutions among sum and difference patterns providing low complexity of the antenna feeding network for uniform thinned arrays is addressed. This procedure uses a hybrid version of the Simulated Annealing algorithm (hybrid SA) to optimize a cost function of radiation characteristics for both sum and difference patterns as peak directivity and side lobe level (SLL) while fixing deep nulls. In this framework, examples of half-wavelength spaced linear arrays from 40 to 120 elements were analyzed, as well as an extension to planar arrays by means of separable distributions was developed. The performance of the method is analyzed with different examples and its potential outlined, showing the ability of fixing deep nulls in both sum and difference patterns which share the s...

Sensors

The present work develops an innovative methodology for fixing deep nulls in radiation patterns o... more The present work develops an innovative methodology for fixing deep nulls in radiation patterns of symmetrical thinned arrays while maintaining a low side lobe level (SLL) and a high directivity, implementing an optimization strategy based on the simulated annealing algorithm (SA). This procedure optimizes a cost function that has a term for each characteristic of the desired radiation pattern and can distinguish between the deep nulls and the filled ones depending on whether they are on the Schelkunoff unit circle or not. Then, a direct extension of the methodology for planar arrays based on the separable distribution procedure is addressed. Consequently, some examples with half-wavelength spacing are presented, where the fixing of one, two, or three deep nulls in arrays of 40, 60, and 80 elements are illustrated as well as an extension to a 40 × 40-element planar array with rectangular grid and rectangular boundary, with two deep nulls fixed on each one of its main axes. Additiona...

Sensors, 2020

In the present work, the theoretical basis of the multiplicity of solutions obtained from an init... more In the present work, the theoretical basis of the multiplicity of solutions obtained from an initial real symmetric distribution is derived. This initial solution is devoted to generating an equivalent pure real shaped-beam pattern for a concrete synthesis scenario. However, these new solutions are not based on real symmetric distributions; hence, not based on the generation of pure real patterns. The bandwidth performances and tolerance to errors provided by the multiple solutions in the array design are analyzed by considering different architectures, also including mutual coupling models and element factor expressions due to accuracy purposes. In addition, a technique to obtain efficient linear arrays by designing resonant structures is addressed. Examples involving both standard linear arrays of half-wavelength cylindrical dipoles and resonant linear arrays generating flat-top beam patterns are reported and discussed. Additionally, an extension to planar arrays performed by mean...

Sensors, 2021

In the present paper, an iterative technique devoted to reproducing efficient footprints with arb... more In the present paper, an iterative technique devoted to reproducing efficient footprints with arbitrary boundaries for planar arrays is addressed. The methodology here depicted is based on exploiting the nature of the continuous aperture distribution by expressing it as a Fourier series of moderately high orders. In this manner, the resulting illumination boundary is defined by a target three-dimensional flat-topped pattern composed of stretching and shrinking modified circular Taylor patterns and the maximum order of the series to obtain a good reconstruction is determined by means of the iterative process. Examples and comparisons with the previous literature were conducted by analyzing square and rectangular contoured beams as test cases. Additionally, interesting potentials regarding space applications from a geostationary satellite are outlined by means of the EuTELSAT (European Telecommunications Satellite Organization) European coverage case study. In such a way, its numerica...

Sensors, 2021

Antenna array pattern reconfiguration is usually achieved by changing the relative amplitudes and... more Antenna array pattern reconfiguration is usually achieved by changing the relative amplitudes and/or phases of the excitation distribution present in the array, at the cost of complex feeding networks. In this work, the mechanical displacement of a parasitic array perpendicular to another array with a single driven element is proposed. Additionally, the antenna is optimized addressing the variation of its response led by changes of the environmental dielectric constant of a surrounding gaseous medium. In such a way, a novel multipurpose antenna of utmost simplicity is obtained. From the computation of the self and mutual impedances, a control of the antenna radiation pattern by means of the induced currents in the parasitic elements is modelled. To illustrate the procedure, the technique will be applied to the variation of the side lobe level of a pencil beam and to obtain a flat-topped broadside beam from the same pencil beam, something with high interest for satellite applications...

International Journal of Molecular Sciences, 2021

Brain tissue may be especially sensitive to electromagnetic phenomena provoking signs of neural s... more Brain tissue may be especially sensitive to electromagnetic phenomena provoking signs of neural stress in cerebral activity. Fifty-four adult female Sprague-Dawley rats underwent ELISA and immunohistochemistry testing of four relevant anatomical areas of the cerebrum to measure biomarkers indicating induction of heat shock protein 70 (HSP-70), glucocorticoid receptors (GCR) or glial fibrillary acidic protein (GFAP) after single or repeated exposure to 2.45 GHz radiation in the experimental set-up. Neither radiation regime caused tissue heating, so thermal effects can be ruled out. A progressive decrease in GCR and HSP-70 was observed after acute or repeated irradiation in the somatosensory cortex, hypothalamus and hippocampus. In the limbic cortex; however, values for both biomarkers were significantly higher after repeated exposure to irradiation when compared to control animals. GFAP values in brain tissue after irradiation were not significantly different or were even lower than ...

Sensors, 2020

In this work, the application of a technique for monitoring changes of the dielectric constant of... more In this work, the application of a technique for monitoring changes of the dielectric constant of the atmosphere caused by the presence of pollution is discussed. The method is based on changes in the reflection coefficient of the device induced by these dielectric constant variations of the surrounding medium. To that end, several Yagi–Uda-like antenna designs with different size limitations were simulated by using a Method-of-Moments software and optimized by means of a simulated annealing strategy. It has been found that the larger the optimal elements of the array are allowed to be, the higher the sensitivity reached. Thus, in a trade-off between sensitivity and moderate length (regarding flexibility purposes), the most promising solution has been built. This prototype has been experimentally tested in presence of an artificial aerosol made of PAO (polyalphaolefin) oil and black carbon inclusions of a size of 0.2 μm. As a result, potentials for developing a measurement procedure...

2015 1st URSI Atlantic Radio Science Conference (URSI AT-RASC), 2015

IEEE Access, 2019

Studies are based on the exposure of Sprague-Dawley rats (250 male and 250 female rats) to electr... more Studies are based on the exposure of Sprague-Dawley rats (250 male and 250 female rats) to electromagnetic fields (EMF) at different frequencies in standing and travelling wave chambers. Values of specific absortion rate (SAR) for all of these experiments were obtained from commercially available FDTD-based simulation software based on numerical phantom animals. An experimental radiation system was developed with a standing-wave cavity which keeps electromagnetic parameters constant while facilitating stress-free exposure of animals to non-thermal radiation. This makes it possible to directly measure the power absorbed by the animal and determine whole-body mean SAR according to weight. All studies using this setup were performed with global system for mobile communication (GSM) radiation at 900 MHz. The simple picrotoxin model made allow to identify morphological signs of neurotoxicity in rat brain tissue. Experiments involving travelling waves were done in a commercial Gigahertz Transverse ElectroMagnetic (GTEM) chamber connected to one or two vector signal generators (to carry single or multiple EMF exposure frequencies). In the diathermy model, rat thyroid and thymus exposed to 2.45 GHz radiation showed visible morphological and immune effects. Cellular stress in the cerebral cortex, the cerebellum or both seems to be more associated with the type of signal than with additive effects of combined frequencies. Finally, some hypothesis related with the future models about the ElectroMagnetic (EM) pollution are established. In an urban environmental that combines the electromagnetic and chemical pollution of environmental particles, cortical excitability, inflammatory response, and cell injury can be modified. INDEX TERMS Bioelectromagnetic effects, electromagnetic radiation effects, specific absorption rate. III. ANIMALS, AND PROTOCOLS. All the experimental animals were male or female Sprague-Dawley rats with an approximate weight of 200-250 gr. They were previously treated, in standard conditions (12/12 h of light/darkness cycle), 22 o C, with food

Oncotarget, 2016

Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous ... more Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous systems. In this study, we investigated the non-thermal SAR (Specific Absorption Rate) in the cerebral or cerebellar hemispheres of rats exposed in vivo to combined electromagnetic field (EMF) signals at 900 and 2450 MHz. Forty rats divided into four groups of 10 were individually exposed or not exposed to radiation in a GTEM chamber for one or two hours. After radiation, we used the Chemiluminescent Enzyme-Linked Immunosorbent Assay (ChELISA) technique to measure cellular stress levels, indicated by the presence of heat shock proteins (HSP) 90 and 70, as well as caspase-3-dependent pre-apoptotic activity in left and right cerebral and cerebellar hemispheres of Sprague Dawley rats. Twenty-four hours after exposure to combined or single radiation, significant differences were evident in HSP 90 and 70 but not in caspase 3 levels between the hemispheres of the cerebral cortex at high SAR levels. In the cerebellar hemispheres, groups exposed to a single radiofrequency (RF) and high SAR showed significant differences in HSP 90, 70 and caspase-3 levels compared to control animals. The absorbed energy and/or biological effects of combined signals were not additive, suggesting that multiple signals act on nervous tissue by a different mechanism.

RICYDE. Revista internacional de ciencias del deporte, 2014

culturas deportivas en las personas adultas, la femenina y la masculina, a través del análisis de... more culturas deportivas en las personas adultas, la femenina y la masculina, a través del análisis de los niveles de práctica (tipo de demanda), tipo de actividad practicada y sus características (frecuencia semanal, gasto mensual, espacio de práctica y modo de organización). La metodología cuantitativa empleada ha consistido en la aplicación de un cuestionario estructurado a una muestra aleatoria de las mujeres y varones mayores adultos entre 30 y 64 años (n=340; 50.3% mujeres, 49.7% hombres) en la Comunidad de Madrid. Las conclusiones obtenidas revelan que un 45.3% de hombres y un 34.5% de mujeres practica deporte; además otro 15.5% de mujeres y 11.7% de hombres camina para hacer ejercicio. Aparecen diferencias de género en las actividades practicadas (x 2 (10)=46.83; p<.001; CC=.442), en el número de horas de práctica (t191=2.29; p<.05; d=.33), en el gasto mensual (t148=3.16; p<.001; d=.69) y en el modo de desplazamiento a la actividad (x 2 (7)=27.44; p<0.001; CC=0.359) aunque la práctica de actividades auto-organizadas es mayoritaria en ambos sexos (80.3% hombres, 63.7% mujeres), principalmente en solitario (62.7% hombres, 50.5% mujeres) y en espacios al aire libre (60.4% hombres, 46.6% mujeres).

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY , 2018

Purpose: The aim of this study was to determine whether exposure to radiation from single or mult... more Purpose: The aim of this study was to determine whether exposure to radiation from single or multiple radio-frequency (RF) signals at 900 and 2450 MHz would induce effects in the RAW 264.7 cell line. Materials and methods: Cell cultures were exposed to single or combined RF for 4, 24, 48, or 72 h in a GTEM electromagnetic test chamber. At the end of the radiation exposure time, viability and cell growth were analyzed by flow cytometry, nitric oxide (NO) production was measured by colorimetry, the expression of HSP70 and TNF-a was ascertained by qPCR, and the phagocytic activity was observed by microscopy. Results: NO production increased after 48 h exposure at 2450 MHz, compared with controls. The group subjected to the combined interaction of two RFs showed an increase of HSP70 after 48 h exposure and a significant increase of NO and TNF-a after 72 h. The phagocytic activity of macrophages decreased in all groups as exposure time increased. Conclusions: Our results indicated a decrease in phagocytic activity and an increase in inflammatory, cytoprotective, and cytotoxic responses in macrophages after continuous and combined exposure of multiple RF signals. Multiple RF interact in everyday life, the immune response in humans is unknown. ARTICLE HISTORY

IEEE Access

Programmed cell death constitutes a fundamental part of the immune response to viral infection. T... more Programmed cell death constitutes a fundamental part of the immune response to viral infection. This process forms part of the host defence mechanism and also enables establishment of biomarkers of disease severity. Natural or anthropogenic sources of microwaves emit energy and may alter the ecology of the SAR-CoV-2 virus (which causes COVID-19 disease) in the environment. Determining the associated effects on the immune system and on the health of hosts with COVID-19 disease is thus important. In this review paper, we consider studies analyzing the influence of electromagnetic fields on innate and acquired immune responses in humans, and above all on preclinical experimental animal models and in vitro models, and we also consider studies analyzing immunity acquired from COVID-19 infections associated with cell death. We focus on the effects of electromagnetic fields and the influence of oxidative stress on stimulation or immunomodulation, the inflammatory response, autoimmunity and the participation of intracellular calcium channels in the immunology of COVID-19 disease. Non-ionizing radiation can activate or reduce the inflammatory response, oxidative stress and the entry of intracellular calcium and can facilitate or reduce cell death. The review of experimental study findings indicates that exposure to non-ionizing radiation can also have a bidirectional effect on the immune system, either slowing down or enhancing the processes that lead to the cell death associated with COVID-19 disease. INDEX TERMS Cell death, COVID-19, immune cells, electromagnetic fields, non-ionizing radiation.

Scientific Reports

Difference far-field patterns represent a way for pin-pointing a target in both azimuth and eleva... more Difference far-field patterns represent a way for pin-pointing a target in both azimuth and elevation, extremely useful in radar applications. At the present work, an innovative method for synthesizing good compromise solutions among sum and difference patterns providing low complexity of the antenna feeding network for uniform thinned arrays is addressed. This procedure uses a hybrid version of the Simulated Annealing algorithm (hybrid SA) to optimize a cost function of radiation characteristics for both sum and difference patterns as peak directivity and side lobe level (SLL) while fixing deep nulls. In this framework, examples of half-wavelength spaced linear arrays from 40 to 120 elements were analyzed, as well as an extension to planar arrays by means of separable distributions was developed. The performance of the method is analyzed with different examples and its potential outlined, showing the ability of fixing deep nulls in both sum and difference patterns which share the s...

Sensors

The present work develops an innovative methodology for fixing deep nulls in radiation patterns o... more The present work develops an innovative methodology for fixing deep nulls in radiation patterns of symmetrical thinned arrays while maintaining a low side lobe level (SLL) and a high directivity, implementing an optimization strategy based on the simulated annealing algorithm (SA). This procedure optimizes a cost function that has a term for each characteristic of the desired radiation pattern and can distinguish between the deep nulls and the filled ones depending on whether they are on the Schelkunoff unit circle or not. Then, a direct extension of the methodology for planar arrays based on the separable distribution procedure is addressed. Consequently, some examples with half-wavelength spacing are presented, where the fixing of one, two, or three deep nulls in arrays of 40, 60, and 80 elements are illustrated as well as an extension to a 40 × 40-element planar array with rectangular grid and rectangular boundary, with two deep nulls fixed on each one of its main axes. Additiona...

Sensors, 2020

In the present work, the theoretical basis of the multiplicity of solutions obtained from an init... more In the present work, the theoretical basis of the multiplicity of solutions obtained from an initial real symmetric distribution is derived. This initial solution is devoted to generating an equivalent pure real shaped-beam pattern for a concrete synthesis scenario. However, these new solutions are not based on real symmetric distributions; hence, not based on the generation of pure real patterns. The bandwidth performances and tolerance to errors provided by the multiple solutions in the array design are analyzed by considering different architectures, also including mutual coupling models and element factor expressions due to accuracy purposes. In addition, a technique to obtain efficient linear arrays by designing resonant structures is addressed. Examples involving both standard linear arrays of half-wavelength cylindrical dipoles and resonant linear arrays generating flat-top beam patterns are reported and discussed. Additionally, an extension to planar arrays performed by mean...

Sensors, 2021

In the present paper, an iterative technique devoted to reproducing efficient footprints with arb... more In the present paper, an iterative technique devoted to reproducing efficient footprints with arbitrary boundaries for planar arrays is addressed. The methodology here depicted is based on exploiting the nature of the continuous aperture distribution by expressing it as a Fourier series of moderately high orders. In this manner, the resulting illumination boundary is defined by a target three-dimensional flat-topped pattern composed of stretching and shrinking modified circular Taylor patterns and the maximum order of the series to obtain a good reconstruction is determined by means of the iterative process. Examples and comparisons with the previous literature were conducted by analyzing square and rectangular contoured beams as test cases. Additionally, interesting potentials regarding space applications from a geostationary satellite are outlined by means of the EuTELSAT (European Telecommunications Satellite Organization) European coverage case study. In such a way, its numerica...

Sensors, 2021

Antenna array pattern reconfiguration is usually achieved by changing the relative amplitudes and... more Antenna array pattern reconfiguration is usually achieved by changing the relative amplitudes and/or phases of the excitation distribution present in the array, at the cost of complex feeding networks. In this work, the mechanical displacement of a parasitic array perpendicular to another array with a single driven element is proposed. Additionally, the antenna is optimized addressing the variation of its response led by changes of the environmental dielectric constant of a surrounding gaseous medium. In such a way, a novel multipurpose antenna of utmost simplicity is obtained. From the computation of the self and mutual impedances, a control of the antenna radiation pattern by means of the induced currents in the parasitic elements is modelled. To illustrate the procedure, the technique will be applied to the variation of the side lobe level of a pencil beam and to obtain a flat-topped broadside beam from the same pencil beam, something with high interest for satellite applications...

International Journal of Molecular Sciences, 2021

Brain tissue may be especially sensitive to electromagnetic phenomena provoking signs of neural s... more Brain tissue may be especially sensitive to electromagnetic phenomena provoking signs of neural stress in cerebral activity. Fifty-four adult female Sprague-Dawley rats underwent ELISA and immunohistochemistry testing of four relevant anatomical areas of the cerebrum to measure biomarkers indicating induction of heat shock protein 70 (HSP-70), glucocorticoid receptors (GCR) or glial fibrillary acidic protein (GFAP) after single or repeated exposure to 2.45 GHz radiation in the experimental set-up. Neither radiation regime caused tissue heating, so thermal effects can be ruled out. A progressive decrease in GCR and HSP-70 was observed after acute or repeated irradiation in the somatosensory cortex, hypothalamus and hippocampus. In the limbic cortex; however, values for both biomarkers were significantly higher after repeated exposure to irradiation when compared to control animals. GFAP values in brain tissue after irradiation were not significantly different or were even lower than ...

Sensors, 2020

In this work, the application of a technique for monitoring changes of the dielectric constant of... more In this work, the application of a technique for monitoring changes of the dielectric constant of the atmosphere caused by the presence of pollution is discussed. The method is based on changes in the reflection coefficient of the device induced by these dielectric constant variations of the surrounding medium. To that end, several Yagi–Uda-like antenna designs with different size limitations were simulated by using a Method-of-Moments software and optimized by means of a simulated annealing strategy. It has been found that the larger the optimal elements of the array are allowed to be, the higher the sensitivity reached. Thus, in a trade-off between sensitivity and moderate length (regarding flexibility purposes), the most promising solution has been built. This prototype has been experimentally tested in presence of an artificial aerosol made of PAO (polyalphaolefin) oil and black carbon inclusions of a size of 0.2 μm. As a result, potentials for developing a measurement procedure...

2015 1st URSI Atlantic Radio Science Conference (URSI AT-RASC), 2015

IEEE Access, 2019

Studies are based on the exposure of Sprague-Dawley rats (250 male and 250 female rats) to electr... more Studies are based on the exposure of Sprague-Dawley rats (250 male and 250 female rats) to electromagnetic fields (EMF) at different frequencies in standing and travelling wave chambers. Values of specific absortion rate (SAR) for all of these experiments were obtained from commercially available FDTD-based simulation software based on numerical phantom animals. An experimental radiation system was developed with a standing-wave cavity which keeps electromagnetic parameters constant while facilitating stress-free exposure of animals to non-thermal radiation. This makes it possible to directly measure the power absorbed by the animal and determine whole-body mean SAR according to weight. All studies using this setup were performed with global system for mobile communication (GSM) radiation at 900 MHz. The simple picrotoxin model made allow to identify morphological signs of neurotoxicity in rat brain tissue. Experiments involving travelling waves were done in a commercial Gigahertz Transverse ElectroMagnetic (GTEM) chamber connected to one or two vector signal generators (to carry single or multiple EMF exposure frequencies). In the diathermy model, rat thyroid and thymus exposed to 2.45 GHz radiation showed visible morphological and immune effects. Cellular stress in the cerebral cortex, the cerebellum or both seems to be more associated with the type of signal than with additive effects of combined frequencies. Finally, some hypothesis related with the future models about the ElectroMagnetic (EM) pollution are established. In an urban environmental that combines the electromagnetic and chemical pollution of environmental particles, cortical excitability, inflammatory response, and cell injury can be modified. INDEX TERMS Bioelectromagnetic effects, electromagnetic radiation effects, specific absorption rate. III. ANIMALS, AND PROTOCOLS. All the experimental animals were male or female Sprague-Dawley rats with an approximate weight of 200-250 gr. They were previously treated, in standard conditions (12/12 h of light/darkness cycle), 22 o C, with food

Oncotarget, 2016

Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous ... more Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous systems. In this study, we investigated the non-thermal SAR (Specific Absorption Rate) in the cerebral or cerebellar hemispheres of rats exposed in vivo to combined electromagnetic field (EMF) signals at 900 and 2450 MHz. Forty rats divided into four groups of 10 were individually exposed or not exposed to radiation in a GTEM chamber for one or two hours. After radiation, we used the Chemiluminescent Enzyme-Linked Immunosorbent Assay (ChELISA) technique to measure cellular stress levels, indicated by the presence of heat shock proteins (HSP) 90 and 70, as well as caspase-3-dependent pre-apoptotic activity in left and right cerebral and cerebellar hemispheres of Sprague Dawley rats. Twenty-four hours after exposure to combined or single radiation, significant differences were evident in HSP 90 and 70 but not in caspase 3 levels between the hemispheres of the cerebral cortex at high SAR levels. In the cerebellar hemispheres, groups exposed to a single radiofrequency (RF) and high SAR showed significant differences in HSP 90, 70 and caspase-3 levels compared to control animals. The absorbed energy and/or biological effects of combined signals were not additive, suggesting that multiple signals act on nervous tissue by a different mechanism.

RICYDE. Revista internacional de ciencias del deporte, 2014

culturas deportivas en las personas adultas, la femenina y la masculina, a través del análisis de... more culturas deportivas en las personas adultas, la femenina y la masculina, a través del análisis de los niveles de práctica (tipo de demanda), tipo de actividad practicada y sus características (frecuencia semanal, gasto mensual, espacio de práctica y modo de organización). La metodología cuantitativa empleada ha consistido en la aplicación de un cuestionario estructurado a una muestra aleatoria de las mujeres y varones mayores adultos entre 30 y 64 años (n=340; 50.3% mujeres, 49.7% hombres) en la Comunidad de Madrid. Las conclusiones obtenidas revelan que un 45.3% de hombres y un 34.5% de mujeres practica deporte; además otro 15.5% de mujeres y 11.7% de hombres camina para hacer ejercicio. Aparecen diferencias de género en las actividades practicadas (x 2 (10)=46.83; p<.001; CC=.442), en el número de horas de práctica (t191=2.29; p<.05; d=.33), en el gasto mensual (t148=3.16; p<.001; d=.69) y en el modo de desplazamiento a la actividad (x 2 (7)=27.44; p<0.001; CC=0.359) aunque la práctica de actividades auto-organizadas es mayoritaria en ambos sexos (80.3% hombres, 63.7% mujeres), principalmente en solitario (62.7% hombres, 50.5% mujeres) y en espacios al aire libre (60.4% hombres, 46.6% mujeres).

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY , 2018

Purpose: The aim of this study was to determine whether exposure to radiation from single or mult... more Purpose: The aim of this study was to determine whether exposure to radiation from single or multiple radio-frequency (RF) signals at 900 and 2450 MHz would induce effects in the RAW 264.7 cell line. Materials and methods: Cell cultures were exposed to single or combined RF for 4, 24, 48, or 72 h in a GTEM electromagnetic test chamber. At the end of the radiation exposure time, viability and cell growth were analyzed by flow cytometry, nitric oxide (NO) production was measured by colorimetry, the expression of HSP70 and TNF-a was ascertained by qPCR, and the phagocytic activity was observed by microscopy. Results: NO production increased after 48 h exposure at 2450 MHz, compared with controls. The group subjected to the combined interaction of two RFs showed an increase of HSP70 after 48 h exposure and a significant increase of NO and TNF-a after 72 h. The phagocytic activity of macrophages decreased in all groups as exposure time increased. Conclusions: Our results indicated a decrease in phagocytic activity and an increase in inflammatory, cytoprotective, and cytotoxic responses in macrophages after continuous and combined exposure of multiple RF signals. Multiple RF interact in everyday life, the immune response in humans is unknown. ARTICLE HISTORY