Amin Shah | Universiti Sains Malaysia (original) (raw)

Papers by Amin Shah

Cogent Biology, 2016

Abstract Caffeic acid (CA) is a well-known natural compound used for both health supplement and m... more Abstract Caffeic acid (CA) is a well-known natural compound used for both health supplement and metabolic diseases. In the present study, single, easy, and quick isolation procedure of caffeic acid was described from rosmarinic acid. A quick crystal formation also was described for highly hygroscopic CA which was spontaneous and stable with lower hygroscopic nature. In addition, the produced crystal CA was highly stable and showed neuroprotective activity with <6 μg/ml in R28 cell line and cytotoxic activity with >100 μg/ml in U87 MG which revealed its dual biological effect for the treatment of Alzheimer’s disease and cancer, respectively.

Authors' original file for figure 6

Applied Ecology and Environmental Research, 2021

The statistical association between the capacity for carbon sequestration and planted Pinus roxbu... more The statistical association between the capacity for carbon sequestration and planted Pinus roxburghii tree diameter classes are not recorded in Pakistan. Through the forest inventory approach, a comprehensive study was conducted in planted P. roxburghii forest in Pakistan's Swat Hindu Kush area to determine the carbon stock (CS) of a live tree, forest floor and soil (SOC) and their association with three diameter classes (6-22, 23-40, 41-60 cm). The results specified that stem density had a polynomial relation with the diameter (R2 =-0.54), while tree height had a linear relationship with the diameter (R2 = 0.73). Association of tree volume, stem biomass, total tree biomass with basal area against diameter classes were positively linear [(R2 = 0.86, 0.80, 0.80) for DBH (41-60 cm)]. As the diameter increases, grass CS reduced while litter and deadwood CS increased significantly. Association of diameter classes with (SOC) at various depths was negatively linearly correlated [R2 =-0.99; for DBH (41-60 cm)], while the topsoil layer (0-20 cm) SOC rose significantly with increase in diameter class. With the increase in tree diameter, the tree age increased definitively (R2 = 0.99). An increase in diameter class ultimately increased CS amount, and the trend was as follows: live tree > soil > forest floor. This study significantly demonstrated the diameter relationship with the tree, forest floor and soil CS of planted P. roxburghii forest.

Steel has been the material of choice for automobile manufacturers. In the recent years material ... more Steel has been the material of choice for automobile manufacturers. In the recent years material such as aluminium and its alloys are taking over the market because of their light weight. The use o ...

Revista Brasileira de Farmacognosia, 2020

Syzygium myrtifolium Walp. (Syzygium campanulatum Korth), Myrtaceae, is locally known as "Kelat p... more Syzygium myrtifolium Walp. (Syzygium campanulatum Korth), Myrtaceae, is locally known as "Kelat paya" in Malaysia. Traditionally, it is used as a remedy for stomach pain. The goal of the present study was to investigate the toxicological potential as well as the antidiarrhoeal and antispasmodic activities of betulinic acid, dimethyl cardamonin and standardized non-formulated and nano-formulated ethanol and supercritical fluid extracts prepared from leaves of S. myrtifolium. The standardized extracts did not produce in vivo toxicity. Both the compounds and standardized extracts showed dose-dependent antidiarrhoeal activity by examining changes in the percentage of liquid stools and percentage of defecation frequency. Dimethyl cardamonin and standardized extracts showed antispasmodic activity on the isolated ileum of guinea pigs. Compared with hyoscine-N-butylbromide, dimethyl cardamonin and standardized extracts produced significant, potent antagonizing activity in ileum contractions induced with acetylcholine. Furthermore, the antagonistic potential of S. myrtifolium active markers against muscarinic type M2 and M3 receptors was investigated by molecular docking.

Australian herbal Insight, 2019

(2019). Metabolic similarity of plant and human: implications for efficacy and regulatory complia... more (2019). Metabolic similarity of plant and human: implications for efficacy and regulatory compliance of herbal therapies, Australian Herbal Insight, 2(1), pp. 028-037

Advances in Integrative Medicine, 2019

Background: Uric acid (UA) and other metabolites are excreted through transporters, organic anion... more Background: Uric acid (UA) and other metabolites are excreted through transporters, organic anion transporters (OATs) or urate transporter 1 (URAT1) in renal proximal tubular cells. Interest in transporters as therapeutics is growing, because impairment of transport activities has been linked to various diseases. However, the effects of herbal medicine have not been fully studied. Methods: We transfected pCMV6-OAT1 or pcDNA3.1-OAT3 into HEK293 cells for overexpression of OAT1 and OAT3, respectively. To over-express URAT1, URAT1 cRNA and linear plasmid DNA were injected into Xenopus oocytes. We tested cytotoxicity with luminoscent signal according to the amount of adenosine triphosphate. Absorption of [14C]-UA and [3H]-estrone sulfate (ES) was confirmed by radioactivity. Results: Uptake of UA was inhibited around 89.4% and 53.7% by K-06 and TJ-68, respectively at 200 g/mL to Xenopus oocytes overexpressing URAT1. Each IC50 value is 59.3 ± 33.5 g/mL, 95.9 ± 36.0 g/mL. In OAT1-overexpressed HEK293 cells, absorption of UA was inhibited by 76.0% with K-06 and by 70.5% with TJ-68; their IC50 values were 49.3 ± 57 g/mL and 132.0 ± 182.6 g/mL, respectively. ES-uptake of OAT3overexpressed HEK293 cells was decreased by 102.7% and 105.0% with K-06 or TJ-68 at 1000 g/ml. IC50 values were 31.5 ± 19.3 g/mL and 63.7 ± 9.7 g/mL. K-06 and TJ-68 did not show cytotoxicity up to 1000 g/mL for 24 hr. Conclusion: Renal transporters act important roles in excreting metabolites such as uric acid. Keishibukurioran (K-06) and Shakuyakukanzoto (TJ-68) can be expected to prevent nephrotoxicity by regulating functions of renal transporters.

Nutrients, Jan 9, 2018

The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liv... more The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of (50% ME of ) has alleviated NAFLD in Sprague⁻Dawley rats. This study aimed to assess the molecular mechanisms of action, and to further evaluate the antiangiogenic effect of this extract NAFLD was induced by eight weeks of high-fat diet, and treatment was applied for four weeks. Antiangiogenic activity was assessed by aortic ring assay and by in vitro tests. Our findings demonstrated that the therapeutic effects of 50% ME among NAFLD rats, were associated with a significant increase in serum adiponectin, reduction in the serum levels of RBP4, vaspin, progranulin, TNF-α, IL-6, and significant downregulation of the hepatic gene expression of PPARγ, SLC10A2, and Collα1. Concomitantly, 50% ME of has exhibited a potent antiangiogenic activity on ring assay, cell migration, vascular endothelial g...

World Applied Sciences Journal

Primula is a medicinal plant bearing flowers which belongs to family Primulaceae. These are low g... more Primula is a medicinal plant bearing flowers which belongs to family Primulaceae. These are low growing herbs. Methanol and cold water extraction methods were used for leaves and roots extractions of Primulavulgaris. Antimicrobial activity ofleaves and roots extracts was evaluated by well diffusion method. Results revealed that P. vulgaris leaves extractexhibit excellent inhibitory effects against Escherichia coli that showed zone of inhibition 18 mm with methanol extract and 14 mm with cold water extract and Pseudomonas aeruginosa showed zone of inhibition 14 mm with methanol extract and 13 mm with cold water extract. Roots extract of P. vulgaris also exhibited inhibitory effects against E. coli showed zone of inhibition 13 mm with methanol extract and 16 mm with cold water extract and P. aeruginosa showed zone of inhibition 13 mm with methanol extract and 14mm with cold water extract while Staphylococcus aureus did not show any inhibitory zone with leaves or roots extracts. This r...

Inorganica Chimica Acta, 2015

Two 5-hydroxyisophthalate complexes of nickel(II), formulated as [Ni(l-Hhip)(2-hepy) 2 ] n (1) an... more Two 5-hydroxyisophthalate complexes of nickel(II), formulated as [Ni(l-Hhip)(2-hepy) 2 ] n (1) and [Ni 2 (l-Hhip) 2 (dap) 4 ] n (2) (H 3 hip = 5-hydroxyisophthalic acid, 2-hepy = 2-(2-hydroxyethyl)pyridine, dap = 1,3-diaminopropane), have been synthesized and characterized by chemical and spectroscopic methods. The molecular structures of the complexes have been determined by single-crystal X-ray diffraction analysis. The Ni(II) centres have distorted octahedral geometries in both crystals. Furthermore, both complexes have 1D chain structures in which the individual chains are linked together via hydrogen bonds to give 3D frameworks. Evaluation of the complexes by the agar diffusion method showed that they have weak antibiotic activities against the tested microorganisms.

Tropical Journal of Pharmaceutical Research, 2015

Purpose: To evaluate the diuretic activity and acute toxicity profile of the crude aqueous extrac... more Purpose: To evaluate the diuretic activity and acute toxicity profile of the crude aqueous extract of Achyranthes aspera using animal models. Methods: Albino rats of either sex were divided into five groups (six animals in each group). The control group received normal saline (10 ml/kg), the reference group received furosemide (10 mg/kg) and the test groups were administered different doses of the crude aqueous extract (10, 30 and 50 mg/kg) by intra-peritoneal route, respectively. At the end of 6 h, urine was collected and total volume of urine excreted by each rat was expressed as ml/6 h/100 g of body weight. pH of fresh urine samples, concentration of urinary sodium and potassium ions, Lipschitz value, diuretic index, saliuretic index and Na+/K+ ratio were also calculated to make comparison among the groups. The acute toxicity of the crude extract was assessed in Albino mice. Results: The findings demonstrated that the crude aqueous extract of the plant showed significant diuretic (p < 0.001), natriuretic (p < 0.001) and kaliuretic (p < 0.001) effects. However, during the course of the study, urinary pH remained unchanged. The diuretic index values for the test groups (III, IV & V) were 2.3, 2.6 and 3.1, respectively. Lipschitz values showed that, at the dose of 50 mg/kg, the crude extract showed 46 % of diuretic activity as compared with furosemide. No toxic effects were observed among Albino mice even at a higher dose of 3000 mg/kg. Conclusion: The crude extract of Achyranthes aspera increases the urine volume and concentration of urinary electrolytes in a dose-dependent manner. Therefore, this plant has a diuretic potential. However, future studies should focus on isolating the phytochemical component(s) responsible for diuresis.

Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 2012

ABSTRACT A series of Zn(II) ferrocenylthiosemicarbazones complexes derived from thiosemicarbazide... more ABSTRACT A series of Zn(II) ferrocenylthiosemicarbazones complexes derived from thiosemicarbazide and 4-methyl-, 4-ethyl-, and 4-phenyl-3-thiosemicarbazide were evaluated for their DNA binding propensity and chemical nuclease activity. The equilibrium binding constants, K b, of the complexes for binding with calf thymus DNA (CT DNA) were in the range of 0.68 × 103 to 2.8 × 104 M−1. The complexes do not intercalate into the nucleobases of CT DNA, as evident from viscosity measurements. They exhibit efficient nuclease activity in the absence of an activating agent and cleave supercoiled DNA into nicked and linear circular forms of DNA at very low concentrations.

Separation Science and Technology, 1986

and-conditions-of-access.pdf This article may be used for research, teaching and private study pu... more and-conditions-of-access.pdf This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, redistribution , reselling , loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Metallomics, 2013

Unsymmetrically substituted sterically tuned Pd(II)–NHC complexes of the general formula [PdCl2(N... more Unsymmetrically substituted sterically tuned Pd(II)–NHC complexes of the general formula [PdCl2(NHC)2] (NHC = 1-allyl-3-methylimidazolin-2-ylidene, 7; 1-allyl-3-butylimidazol-2-ylidene, 8; 1-benzyl-3-butyl imidazolin-2-ylidene, 9) were prepared through transmetallation from their corresponding Ag(I)–NHC complexes. The Pd complexes were structurally characterized by different spectroscopic and X-ray diffraction methods. Complexes 7 and 9 adopted a trans–anti arrangement of the NHC ligands, whereas complex 8 adopted a cis–syn arrangement. Preliminary antibiogram studies using Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria showed that Ag(I)–NHC complexes demonstrate higher activity compared with Pd(I)–NHC complexes. Furthermore, Pd(II)–NHC complexes were evaluated for their anticancer potential using the human colorectal cancer cell line. A higher anticancer activity was observed for complexes 8 and 9, with 26.5 and 6.6 mM IC50 values, respectively.

Chemistry Central Journal, 2013

Background Since the first successful synthesis of Ag(I)-N-heterocyclic carbene complex in 1993, ... more Background Since the first successful synthesis of Ag(I)-N-heterocyclic carbene complex in 1993, this class of compounds has been extensively used for transmetallation reactions where the direct synthesis using other metal ions was either difficult or impossible. Initially, silver(I)-NHC complexes were tested for their catalytic potential but could not get fame because of lower potential compare to other competent compounds in this field; however, these compounds proved to have vital antimicrobial activities. These encouraging biomedical applications further convinced researchers to test these compounds against cancer. The current work has been carried out with this aim. Results N-ipropylbenzimidazole was synthesized by reaction of benzimidazole with i propyl bromide. The subsequent treatment of the resulting N-alkylbenzimidazole with ortho/meta/para-(bromomethylene) benzene afforded corresponding bis-benzimidazolium bromides (5-7). The counter anion (Br-) of each salt was replaced ...

BMC Complementary and Alternative Medicine, 2013

Background: Syzygium campanulatum Korth (Myrtaceae) is an evergreen shrub rich in phenolics, flav... more Background: Syzygium campanulatum Korth (Myrtaceae) is an evergreen shrub rich in phenolics, flavonoid antioxidants, and betulinic acid. This study sought to investigate antiangiogenic and anti-colon cancer effects of S.C. standardized methanolic extract. Methods: Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S. C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LC-MS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results: Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC 50 = 17.6 ± 2.9 μg/ml). S. C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion: Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid.

Abstract: The present invention refers to a use of an effective amount of a composition comprisin... more Abstract: The present invention refers to a use of an effective amount of a composition comprising O stamineus leaf extract More particularly, the present invention relates to a phytochemical and pharmacological composition comprising standardized Orthosiphon stamineus leaf extract that inhibits angiogenesis by blocking VEGF receptor-2 tyrosine kinase pathway and enhancing therapeutic potency of tamoxifen The O stamineus leaf extract of the present invention inhibits angiogenesis by blocking VEGF signaling pathway ...

Orthosiphon stamineus Benth. is a medicinal herb belonging to the family Lamiaceae, grown in Sout... more Orthosiphon stamineus Benth. is a medicinal herb belonging to the family Lamiaceae, grown in Southeast Asia. Leaves of this plant are used commonly in Southeast Asia and European countries for herbal tea, well known as “Java tea”. Traditionally leaves of this plant have been used as diuretic, and to treat rheumatism, abdominal pain, kidney and bladder inflammation, edema and gout. Studies have shown that the O. stamineus leaves exhibit a range of pharmacological properties such as, anti-inflammatory, antioxidant, anti- ...