norzila ismail | Universiti Sains Malaysia (original) (raw)
Address: Kota Bharu, Kelantan, Malaysia
less
Uploads
Papers by norzila ismail
Natural products from plant are still the alternative source in the search for anticancer drugs t... more Natural products from plant are still the alternative source in the search for anticancer drugs that can have a direct effect on both cancer cell removal and also minimize the side effects to patients. The cytotoxicity actions of anticancer compounds coupled with the ability to activate anticancer immune responses would be an ideal strategy to combat the disease. Recently, we have isolated a flavonoid from Brucea javanica leaves which has shown relatively potent cytotoxic activity in several cancer cell lines. However, it is not known if this compound has any potential to modulate the activity of immune cells. The flavonoid fraction from Brucea javanica (BJLM-C8) was derived through bioassay-guided fractionation using methylene blue assay in several cancer cell lines: CaOV-3, HeLa, MDA-MB 231, HepG2, MG63 and non-cancer cell lines Vero and L929. The flowcytometric analysis revealed that BJLM-C8 has the ability to induce apoptosis by increasing the bax, p53 and caspase-3 protein expression. It also decreases the expression of pro-inflammatory cytokines TNFα and IL-1β in human blood culture that stimulated with 10µg/ml Lipopolysaccharide. Besides, the IFNγ and IL-2 were increased in a mixed lymphocytes culture after being treated BJLM-C8 which reflects its anticancer immune response. It was also found to activate Natural Killer cells as it slightly increased the K562 cell death in co-culture experiment.
Natural products from plant are still the alternative source in the search for anticancer drugs t... more Natural products from plant are still the alternative source in the search for anticancer drugs that can have a direct effect on both cancer cell removal and also minimize the side effects to patients. The cytotoxicity actions of anticancer compounds coupled with the ability to activate anticancer immune responses would be an ideal strategy to combat the disease. Recently, we have isolated a flavonoid from Brucea javanica leaves which has shown relatively potent cytotoxic activity in several cancer cell lines. However, it is not known if this compound has any potential to modulate the activity of immune cells. The flavonoid fraction from Brucea javanica (BJLM-C8) was derived through bioassay-guided fractionation using methylene blue assay in several cancer cell lines: CaOV-3, HeLa, MDA-MB 231, HepG2, MG63 and non-cancer cell lines Vero and L929. The flowcytometric analysis revealed that BJLM-C8 has the ability to induce apoptosis by increasing the bax, p53 and caspase-3 protein expression. It also decreases the expression of pro-inflammatory cytokines TNFα and IL-1β in human blood culture that stimulated with 10µg/ml Lipopolysaccharide. Besides, the IFNγ and IL-2 were increased in a mixed lymphocytes culture after being treated BJLM-C8 which reflects its anticancer immune response. It was also found to activate Natural Killer cells as it slightly increased the K562 cell death in co-culture experiment.