Gilberto Sabino-Santos Jr | Universidade de São Paulo (original) (raw)
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Papers by Gilberto Sabino-Santos Jr
We screened blood samples from 560 wild rodents collected in southeastern Brazil for antibodies t... more We screened blood samples from 560 wild rodents collected in southeastern Brazil for antibodies to a recombinant nucleoprotein (rN) of Jun ́ın virus. Six rodents were antibody positive (1.1%), demonstrating evidence of infection with mammarenaviruses in several species of Brazilian rodents.
Cardiopulmonary Syndrome (HCPS) is a severe disease caused by Hantaviruses. In the present study,... more Cardiopulmonary Syndrome (HCPS) is a severe disease caused by Hantaviruses. In the present study, we have analyzed sera of 62 HCPS suspected cases from the region of Ribeirão Preto, São Paulo, in the period of 2010-2011. Clinical samples of twelve patients were positive, based on both RT-PCR and IgM-ELISA. Hantavirus-infected patients included 8 males and 4 females, 16-57 years old, 3 of whom were rural workers (25%) and one of them related direct contact with wild rodents. The majority of the other patients (75%) lived in the periphery of cities and reported the presence of rodents in their homes. The case fatality ratio of these 12 HCPS cases was 41.6%. Our results confirm that hantaviruses are endemic, with occurrence of HCPS and fatalities, every year, in the region of Ribeirão Preto. More educational and preventive measures are necessary in order to prevent human infections by hantavirus in the region, and other parts of Brazil.
Introduction: In recent years, hantavirus infections producing severe diseases have obtained an i... more Introduction: In recent years, hantavirus infections producing severe diseases have obtained an increased a ention from public health authorities from the countries of Eurasia to the Americas. Brazil has reported 1,300 cases of hantavirus cardiopulmonary syndrome (HCPS) from 1993 to 2010, with about 80 of them occurring in the northeast of the State of São Paulo, with 48% fatality rate. Araraquara virus was the causative agent of HCPS in the region. Considering that hantaviruses causing human disease in the Americas were unknown until 1993, we have looked for hantavirus infections in the population of Cássia dos Coqueiros county, northeast of the State of São Paulo, Brazil, before this time. is county has about 2,800 inhabitants and an economy based on agriculture, including cultivation of Brachiaria decumbens grass. e grass seeds are an important rodent a raction, facilitating transmission of hantavirus to man. Four HCPS cases were reported so far in the county. Methods: In this study, 1,876 sera collected from 1987 to 1990 were tested for IgG to hantavirus by IgG-ELISA, using the N recombinant protein of Araraquara virus as antigen. Results: Positive results were observed in 89 (4.7%) samples, which were all collected in 1987. e positivity among urban inhabitants was 5.3%, compared with 4.3% among those living in rural areas. Conclusions: Our results showed that hantavirus infections occurred in Cássia dos Coqueiros, completely unrecognized, even before hantaviruses were described in the Americas.
Introduction: Rodent-borne hantaviruses cause severe human diseases. We completed a serological s... more Introduction: Rodent-borne hantaviruses cause severe human diseases. We completed a serological survey of hantavirus infection in rural inhabitants of Turvo County, in the southern State of Santa Catarina, Brazil, in which seropositivity for hantavirus was correlated to previous disease in the participants. Methods: The levels of IgG antibodies to hantavirus Araraquara in the sera of 257 individuals were determined using an immunoenzymatic assay. Results: IgG antibodies to hantavirus were found in 2.3% of the participants. All seropositive participants reported previous disease with symptoms suggestive of hantavirus cardiopulmonary syndrome. Conclusions: Human infections causing unreported cardiopulmonary syndrome probably occur in the southern State of Santa Catarina.
Introduction: Hantavirus pulmonary and cardiovascular syndrome (HPCS) is an emerging serious dise... more Introduction: Hantavirus pulmonary and cardiovascular syndrome (HPCS) is an emerging serious disease in the Americas. Hantaviruses (Bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. In the Ribeirão Preto region (state of São Paulo, Brazil), HPCS has been reported since 1998, caused by the Araraquara virus (ARAV), for which Necromys lasiurus is the rodent reservoir. This study aimed to show diagnostic results relating to infection in humans and rodents, obtained at the Virology Research Center of the Ribeirão Preto School of Medicine, University of São Paulo, between 2005 and 2008. Methods: HPCS was diagnosed by means of ELISA and/or RT-PCR in 11 (21.2%) out of 52 suspected cases, and 54.4% of these were fatal. Furthermore, 595 wild rodents (Necromys lasiurus, Akodon sp, Calomys tener and Oligoryzomys sp) were caught between 2005 and 2008. Results: Fifteen (2.5%) of these rodents presented antibodies for hantavirus, as follows: Necromys lasiurus (4%), Calomys tener (1.9%) and Akodon sp (1.5%). Nucleotide sequences obtained through RT-PCR from one HPCS patient and one Calomys tener rodent were compared with hantavirus sequences from GenBank, which showed that both were homologous with ARAV. Conclusions: This work corroborates previous studies showing that ARAV is the hantavirus causing HPCS in the Ribeirão Preto region. It also shows that rodents infected with hantavirus represent a constant risk of transmission of this virus to man.
Infections by Hantavirus (Bunyaviridae) can cause severe human diseases, such as hemorrhagic feve... more Infections by Hantavirus (Bunyaviridae) can cause severe human diseases, such as hemorrhagic fever with renal syndrome in Eurasia and cardiopulmonary syndrome in the Americas. These diseases are emergent and became a serious public health problem worldwide. Thus, rapid, sensitive and reliable methods for diagnosis of hantavirus infection are necessary in order to manage patients and control this rodent-borne virosis. Serological methods, such as neutralization tests, immunoblots and enzyme immunoassays using hantavirus-recombinant proteins as antigens, are discussed in this article, as well new methods such as immunochromatographic test. Hantavirus genome detection, by different kinds of reverse transcription-PCR, including the real-time variant, is also discussed here.
From February 2009 to February 2010 the small mammal assemblage in sugarcane fields from Usina Sa... more From February 2009 to February 2010 the small mammal assemblage in sugarcane fields from Usina Santa Elisa Vale, in Sertãozinho, northern São Paulo state (Brazil), with four different time spans of pre- harvest burning suspension (0, 3, 5 and 10 years) was surveyed. Six species of rodents and one specie of marsupial were captured, in a total of 399 individuals, with a total capture success of 7.7%. Species richness was similar in all treatments; however, the marsupial Gracilinanus agilis was only detected in sugarcane plots where pre-harvest burning has been suspended for 10 years. In addition, small rodents were more abundant in plots where pre-harvest burning was still used every year, followed by areas where fire events were suppressed for 10 years. Considering that high densities of small rodents are usually associated with the spread of infectious diseases (e.g., hantaviroses and leptospirosis) and also with the possible use of highly polluting agrochemicals (i.e., rodenticides), a decrease in small rodents abundance can be considered another positive environmental impact of pre-harvest burning suspension of sugarcane fields.
Hantaviruses are zoonotic viruses harbored by rodents, bats, and shrews. At present, only rodent-... more Hantaviruses are zoonotic viruses harbored by rodents, bats, and shrews. At present, only rodent-borne hantaviruses are associated with severe illness in humans. New species of hantaviruses have been recently identified in bats and shrews greatly expanding the potential reservoirs and ranges of these viruses. Brazil has one of the highest incidences of hantavirus cardiopulmonary syndrome in South America, hence it is critical to know what is the prevalence of hantaviruses in Brazil. Although much is known about rodent reservoirs, little is known regarding bats. We captured 270 bats from February 2012 to April 2014. Serum was screened for the presence of antibodies against a recombinant nucleoprotein (rN) of Araraquara virus (ARAQV). The prevalence of antibody to hantavirus was 9/53 with an overall seroprevalence of 17%. Previous studies have shown only insectivorous bats to harbor hantavirus; however, in our study, of the nine seropositive bats, five were frugivorous, one was carnivorous, and three were sanguivorous phyllostomid bats.
Other by Gilberto Sabino-Santos Jr
We screened blood samples from 560 wild rodents collected in southeastern Brazil for antibodies t... more We screened blood samples from 560 wild rodents collected in southeastern Brazil for antibodies to a recombinant nucleoprotein (rN) of Jun ́ın virus. Six rodents were antibody positive (1.1%), demonstrating evidence of infection with mammarenaviruses in several species of Brazilian rodents.
Cardiopulmonary Syndrome (HCPS) is a severe disease caused by Hantaviruses. In the present study,... more Cardiopulmonary Syndrome (HCPS) is a severe disease caused by Hantaviruses. In the present study, we have analyzed sera of 62 HCPS suspected cases from the region of Ribeirão Preto, São Paulo, in the period of 2010-2011. Clinical samples of twelve patients were positive, based on both RT-PCR and IgM-ELISA. Hantavirus-infected patients included 8 males and 4 females, 16-57 years old, 3 of whom were rural workers (25%) and one of them related direct contact with wild rodents. The majority of the other patients (75%) lived in the periphery of cities and reported the presence of rodents in their homes. The case fatality ratio of these 12 HCPS cases was 41.6%. Our results confirm that hantaviruses are endemic, with occurrence of HCPS and fatalities, every year, in the region of Ribeirão Preto. More educational and preventive measures are necessary in order to prevent human infections by hantavirus in the region, and other parts of Brazil.
Introduction: In recent years, hantavirus infections producing severe diseases have obtained an i... more Introduction: In recent years, hantavirus infections producing severe diseases have obtained an increased a ention from public health authorities from the countries of Eurasia to the Americas. Brazil has reported 1,300 cases of hantavirus cardiopulmonary syndrome (HCPS) from 1993 to 2010, with about 80 of them occurring in the northeast of the State of São Paulo, with 48% fatality rate. Araraquara virus was the causative agent of HCPS in the region. Considering that hantaviruses causing human disease in the Americas were unknown until 1993, we have looked for hantavirus infections in the population of Cássia dos Coqueiros county, northeast of the State of São Paulo, Brazil, before this time. is county has about 2,800 inhabitants and an economy based on agriculture, including cultivation of Brachiaria decumbens grass. e grass seeds are an important rodent a raction, facilitating transmission of hantavirus to man. Four HCPS cases were reported so far in the county. Methods: In this study, 1,876 sera collected from 1987 to 1990 were tested for IgG to hantavirus by IgG-ELISA, using the N recombinant protein of Araraquara virus as antigen. Results: Positive results were observed in 89 (4.7%) samples, which were all collected in 1987. e positivity among urban inhabitants was 5.3%, compared with 4.3% among those living in rural areas. Conclusions: Our results showed that hantavirus infections occurred in Cássia dos Coqueiros, completely unrecognized, even before hantaviruses were described in the Americas.
Introduction: Rodent-borne hantaviruses cause severe human diseases. We completed a serological s... more Introduction: Rodent-borne hantaviruses cause severe human diseases. We completed a serological survey of hantavirus infection in rural inhabitants of Turvo County, in the southern State of Santa Catarina, Brazil, in which seropositivity for hantavirus was correlated to previous disease in the participants. Methods: The levels of IgG antibodies to hantavirus Araraquara in the sera of 257 individuals were determined using an immunoenzymatic assay. Results: IgG antibodies to hantavirus were found in 2.3% of the participants. All seropositive participants reported previous disease with symptoms suggestive of hantavirus cardiopulmonary syndrome. Conclusions: Human infections causing unreported cardiopulmonary syndrome probably occur in the southern State of Santa Catarina.
Introduction: Hantavirus pulmonary and cardiovascular syndrome (HPCS) is an emerging serious dise... more Introduction: Hantavirus pulmonary and cardiovascular syndrome (HPCS) is an emerging serious disease in the Americas. Hantaviruses (Bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. In the Ribeirão Preto region (state of São Paulo, Brazil), HPCS has been reported since 1998, caused by the Araraquara virus (ARAV), for which Necromys lasiurus is the rodent reservoir. This study aimed to show diagnostic results relating to infection in humans and rodents, obtained at the Virology Research Center of the Ribeirão Preto School of Medicine, University of São Paulo, between 2005 and 2008. Methods: HPCS was diagnosed by means of ELISA and/or RT-PCR in 11 (21.2%) out of 52 suspected cases, and 54.4% of these were fatal. Furthermore, 595 wild rodents (Necromys lasiurus, Akodon sp, Calomys tener and Oligoryzomys sp) were caught between 2005 and 2008. Results: Fifteen (2.5%) of these rodents presented antibodies for hantavirus, as follows: Necromys lasiurus (4%), Calomys tener (1.9%) and Akodon sp (1.5%). Nucleotide sequences obtained through RT-PCR from one HPCS patient and one Calomys tener rodent were compared with hantavirus sequences from GenBank, which showed that both were homologous with ARAV. Conclusions: This work corroborates previous studies showing that ARAV is the hantavirus causing HPCS in the Ribeirão Preto region. It also shows that rodents infected with hantavirus represent a constant risk of transmission of this virus to man.
Infections by Hantavirus (Bunyaviridae) can cause severe human diseases, such as hemorrhagic feve... more Infections by Hantavirus (Bunyaviridae) can cause severe human diseases, such as hemorrhagic fever with renal syndrome in Eurasia and cardiopulmonary syndrome in the Americas. These diseases are emergent and became a serious public health problem worldwide. Thus, rapid, sensitive and reliable methods for diagnosis of hantavirus infection are necessary in order to manage patients and control this rodent-borne virosis. Serological methods, such as neutralization tests, immunoblots and enzyme immunoassays using hantavirus-recombinant proteins as antigens, are discussed in this article, as well new methods such as immunochromatographic test. Hantavirus genome detection, by different kinds of reverse transcription-PCR, including the real-time variant, is also discussed here.
From February 2009 to February 2010 the small mammal assemblage in sugarcane fields from Usina Sa... more From February 2009 to February 2010 the small mammal assemblage in sugarcane fields from Usina Santa Elisa Vale, in Sertãozinho, northern São Paulo state (Brazil), with four different time spans of pre- harvest burning suspension (0, 3, 5 and 10 years) was surveyed. Six species of rodents and one specie of marsupial were captured, in a total of 399 individuals, with a total capture success of 7.7%. Species richness was similar in all treatments; however, the marsupial Gracilinanus agilis was only detected in sugarcane plots where pre-harvest burning has been suspended for 10 years. In addition, small rodents were more abundant in plots where pre-harvest burning was still used every year, followed by areas where fire events were suppressed for 10 years. Considering that high densities of small rodents are usually associated with the spread of infectious diseases (e.g., hantaviroses and leptospirosis) and also with the possible use of highly polluting agrochemicals (i.e., rodenticides), a decrease in small rodents abundance can be considered another positive environmental impact of pre-harvest burning suspension of sugarcane fields.
Hantaviruses are zoonotic viruses harbored by rodents, bats, and shrews. At present, only rodent-... more Hantaviruses are zoonotic viruses harbored by rodents, bats, and shrews. At present, only rodent-borne hantaviruses are associated with severe illness in humans. New species of hantaviruses have been recently identified in bats and shrews greatly expanding the potential reservoirs and ranges of these viruses. Brazil has one of the highest incidences of hantavirus cardiopulmonary syndrome in South America, hence it is critical to know what is the prevalence of hantaviruses in Brazil. Although much is known about rodent reservoirs, little is known regarding bats. We captured 270 bats from February 2012 to April 2014. Serum was screened for the presence of antibodies against a recombinant nucleoprotein (rN) of Araraquara virus (ARAQV). The prevalence of antibody to hantavirus was 9/53 with an overall seroprevalence of 17%. Previous studies have shown only insectivorous bats to harbor hantavirus; however, in our study, of the nine seropositive bats, five were frugivorous, one was carnivorous, and three were sanguivorous phyllostomid bats.