Luciana Saraiva | Universidade de São Paulo (original) (raw)

Papers by Luciana Saraiva

Research paper thumbnail of Influence of Piezosurgery on Bone Healing around Titanium Implants: A Histological Study in Rats

Brazilian Dental Journal, 2016

The aim of this study was to evaluate histomorphometrically the influence of two techniques of de... more The aim of this study was to evaluate histomorphometrically the influence of two techniques of dental implant site preparation on bone healing around titanium implants. Fifteen male Wistar rats (±300 g) were used in the study. Each tibia was randomly assigned to receive the implant site preparation either with a conventional drilling technique (control - DRILL group) or with a piezoelectric device (PIEZO group). The animals were sacrificed after 30 days and then the following histomorphometric parameters were evaluated (percentage) separately for cortical and cancellous regions: proportion of mineralized tissue (PMT) adjacent to implant threads (500 μm adjacent); bone area within the threads (BA) and bone-implant contact (BIC). The results demonstrated that there were no statistically significant differences between both groups for cancellous BIC (p>0.05) and cortical PMT (p>0.05). On the other hand, a higher percentage of BA was observed in the PIEZO group in the cortical (71...

Research paper thumbnail of Oral and Fecal Microbiome in Molar-Incisor Pattern Periodontitis

Frontiers in Cellular and Infection Microbiology

Research paper thumbnail of Impacto da periodontite agressiva e da periodontite crônica na qualidade de vida relacionada à saúde bucal

Brazilian Journal of Implantology and Health Sciences

O objetivo deste estudo transversal foi investigar o efeito de diferentes formas de doenças perio... more O objetivo deste estudo transversal foi investigar o efeito de diferentes formas de doenças periodontais na qualidade de vida relacionada à saúde bucal (OHRQoL). Foram incluídos 52 pacientes com Periodontite Agressiva (AP) ou Periodontite Crônica (PC): nove pacientes com Periodontite Agressiva Localizada (LAP), trinta e três pacientes com Periodontite Agressiva Generalizada (GAP) e dez pacientes com Periodontite Crônica Generalizada (GCP) . Os questionários de Perfil de Impacto na Saúde Oral (OHIP-14) foram distribuídos após um exame clínico que mediu os seguintes parâmetros periodontais: perda dentária, sangramento na sondagem (BoP), profundidade da sondagem (DP), recessão gengival (REC) e nível de inserção clínica (CAL) ) As médias globais da pontuação do OHIP-14 foram 10,6 para o LAP, 16,5 para o GAP e 17,5 para o GCP. Diferença estatisticamente significante (p <0. 01) foi observado entre o grupo LAP e os outros dois grupos. Houve significativamente menos sangramento e recessã...

Research paper thumbnail of Chemokines and cytokines profile in whole saliva of patients with periodontitis

Research paper thumbnail of Effect of periodontal treatment on Aggregatibacter actinomycetemcomitans (Aa) colonization and serum IgG levels against Aa serotypes and Omp29 of aggressive periodontitis patients

Research paper thumbnail of A systematic review and meta-analysis of the survival rate of implants placed in previously failed sites

Brazilian oral research, 2018

The aim of this study was to conduct a systematic review and meta-analysis to assess the clinical... more The aim of this study was to conduct a systematic review and meta-analysis to assess the clinical outcomes of dental implants placed in previously early and late implant failed sites. An electronic literature search was conducted in several databases for articles published up to February 2018. Human clinical trials that received at least one implant in a previously failed site were included. Hence, the PICO question that was aimed to be addressed was: Do patients undergoing implant replacement (second and third attempts) in previous failed sites have survival rates similar to implants placed at first attempts? A random effects model was used to calculate survival weighted means and corresponding 95% Confidence Intervals (CI) among studies. Eleven studies of low to moderate methodological quality were included in this review. Implants placed in sites with history of one and two implant failures had a weighted survival rate (SR) of 88.7% (95%CI 81.7-93.3) and 67.1% (95%CI 51.1-79.9), ...

Research paper thumbnail of The ideal time of systemic metronidazole and amoxicillin administration in the treatment of severe periodontitis: study protocol for a randomized controlled trial

Trials, Jan 27, 2018

The combination of systemic metronidazole (MTZ) and amoxicillin (AMX) with scaling and root plani... more The combination of systemic metronidazole (MTZ) and amoxicillin (AMX) with scaling and root planing (SRP) has shown to be an effective periodontal treatment. However, some essential issues associated with the use of these antibiotics remain unanswered, such as the ideal time of administration during the course of periodontal treatment. Although these agents are often prescribed after the healing phase of the SRP procedure, there is biological plausibility to support its use in conjunction with the mechanical treatment. However, to date, no placebo controlled randomized clinical trial (RCT) has directly compared these two protocols. Therefore, the aim of this RCT is to compare the clinical, microbiological and immunological effects of the adjunctive systemic MTZ + AMX administered in different phases of the treatment of severe periodontitis. Subjects with severe periodontitis (n = 180) are being randomly assigned into three groups (n = 60/group): (i) SRP-only (control group), SRP in ...

Research paper thumbnail of Impact of aggressive periodontitis and chronic periodontitis on oral health-related quality of life

Brazilian oral research, 2018

The purpose of this cross-sectional study was to investigate the effect of different forms of per... more The purpose of this cross-sectional study was to investigate the effect of different forms of periodontal diseases on Oral Health-Related Quality of Life (OHRQoL). Fifty-two patients with Aggressive Periodontitis (AP) or Chronic Periodontitis (CP) were included: nine patients with Localized Aggressive Periodontitis (LAP), thirty-three patients with Generalized Aggressive Periodontitis (GAP) and ten patients with Generalized Chronic Periodontitis (GCP). Oral Health Impact Profile questionnaires (OHIP-14) were distributed after a clinical examination that measured the following periodontal parameters: tooth loss, bleeding on probing (BoP), probing depth (PD), gingival recession (REC) and clinical attachment level (CAL). The global OHIP-14 score means were 10.6 for LAP, 16.5 for GAP, and 17.5 for GCP. A statistically significant difference (p < 0.01) was observed between the LAP group and the other two groups. There was significantly less bleeding and recession in the LAP group than...

Research paper thumbnail of PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity

Brazilian Oral Research, 2017

Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association be... more Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

Research paper thumbnail of Effects of periodontal treatment on primary sjȫgren’s syndrome symptoms

Brazilian Oral Research, 2017

The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatme... more The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (ESSDAI) and subjective (ESSPRI) indexes in patients with primary Sjögren's Syndrome (pSS). Twenty-eight female patients were divided into four groups: pSS patients with or without chronic periodontitis (SCP, SC, respectively), and systemically healthy patients with or without chronic periodontitis (CP, C, respectively). Periodontal clinical examination and immunological and microbiological sample collection were performed at baseline, 30 and 90 days after nonsurgical periodontal treatment (NSPT). Levels of interleukin IL-1β, IL-8 and IL-10 in saliva and gingival crevicular fluid (GCF) were evaluated by ELISA, as well as the expression of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans, (Aa) Tannerella forsythia (Tf), and Treponema denticola (Td), by qPCR. Systemic activity and pSS symptoms were evaluated by ESSDAI and ESSPRI. NSPT resulted in improved periodontal clinical parameters in both SCP and CP groups (p>0.05). Pg, Aa, and Tf levels decreased after NSPT only in CP patients (p<0.05). Significantly greater levels of IL-10 in GCF were verified in both SCP and CP groups (p<0.05). SCP patients showed increased salivary flow rates and decreased ESSPRI scores after NSPT. In conclusion, NSPT in pSS patients resulted in improved clinical and immunological parameters, with no significant effects on microbiological status. pSS patients also showed increased salivary flow and lower ESSPRI scores after therapy. Therefore, it can be suggested that NSPT may improve the quality of life of pSS patients.

Research paper thumbnail of Perfil De Morbidade Dos Usuários Da Divisão De Assistência a Saúde Do Departamento De Assistência Ao Servidor Da PRH/Ufrn

Fiep Bulletin on Line, 2009

Research paper thumbnail of Diagnóstico clínico e complementar das doenças periimplantares

Periodontia, 2008

Base de dados : LILACS. Pesquisa : 544277 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 544277 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 544277. ...

Research paper thumbnail of Gingival Overgrowth Among Renal Transplant Patients Medicated with Cyclosporin and Tacrolimus: Prospective Study

Research paper thumbnail of Incidence of tacrolimus-induced gingival overgrowth in the absence of calcium channel blockers: a short-term study

Journal of Clinical Periodontology, 2007

The aim of this study was to determine the incidence and severity of gingival overgrowth (GO) ind... more The aim of this study was to determine the incidence and severity of gingival overgrowth (GO) induced by tacrolimus (Tcr) compared with ciclosporin A (CiA) in the absence of calcium channel blockers (CCB) in renal transplant recipients. Methods: Forty patients (20 Tcr and 20 CiA) were evaluated before and 30 and 90 days after kidney transplantation. Demographic (age, gender) and periodontal parameters were recorded for all patients. Patients taking CCB at any time during the study were excluded from the investigation. Results: The mean GO score was significantly lower (p 5 0.014) in the Tcr group (6.4%) compared with the CiA group (17.9%) after 90 days of immunosuppressive therapy. At 90 days post-transplant, clinically significant GO was observed in four patients of the CiA group and in two of the Tcr group. This difference was not statistically significant (0.66). Conclusion: No significant difference in the incidence of clinically significant GO was observed between the CiA and Tcr groups up to 90 days of immunosuppressive therapy.

Research paper thumbnail of Orthodontic treatment in patients with aggressive periodontitis

Introduction: Aggressive periodontitis (AP) is a condition that promotes breakdown of the periodo... more Introduction: Aggressive periodontitis (AP) is a condition that promotes breakdown of the periodontal tissues in a short time. In severe cases, pathologic migration of teeth and tooth loss can occur, producing esthetic and functional problems for the patient. Orthodontic treatment may be recommended to restore esthetics and masticatory function. We assessed the effects of orthodontic movement in the periodontal tissues of treated patients with AP. Methods: Ten subjects (ages 25.0 6 5.22 years) with AP received periodontal treatment followed by orthodontic treatment. Maintenance sessions were performed monthly under a strict dental biofilm control. They were compared with 10 periodontally healthy subjects (ages 22.9 6 5.23 years) who received orthodontic treatment. Probing pocket depth, clinical attachment level, bleeding on probing, and dental plaque index were measured at baseline, after orthodontic treatment, and after 4 months. Results: Statistical analysis showed improvement in ...

Research paper thumbnail of Influence of Piezosurgery on Bone Healing around Titanium Implants: A Histological Study in Rats

Brazilian Dental Journal, 2016

The aim of this study was to evaluate histomorphometrically the influence of two techniques of de... more The aim of this study was to evaluate histomorphometrically the influence of two techniques of dental implant site preparation on bone healing around titanium implants. Fifteen male Wistar rats (±300 g) were used in the study. Each tibia was randomly assigned to receive the implant site preparation either with a conventional drilling technique (control - DRILL group) or with a piezoelectric device (PIEZO group). The animals were sacrificed after 30 days and then the following histomorphometric parameters were evaluated (percentage) separately for cortical and cancellous regions: proportion of mineralized tissue (PMT) adjacent to implant threads (500 μm adjacent); bone area within the threads (BA) and bone-implant contact (BIC). The results demonstrated that there were no statistically significant differences between both groups for cancellous BIC (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05) and cortical PMT (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). On the other hand, a higher percentage of BA was observed in the PIEZO group in the cortical (71.50±6.91 and 78.28±4.38 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05) and cancellous regions (9.62±4.06 and 19.94±14.18 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). The piezosurgery also showed higher PMT values in the cancellous zone (9.35±5.54 and 18.72±13.21 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). However, the DRILL group presented better results for BIC in cortical region (80.42±10.88 and 70.25±16.93 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). In conclusion, for the implant site preparation, the piezosurgery was beneficial to bone healing rates in the cancellous bone region, while the drill technique produced better results in the cortical bone.

Research paper thumbnail of IgG sera levels against a subset of periodontopathogens and severity of disease in aggressive periodontitis patients: A cross-sectional study of selected pocket sites

Aims: To evaluate the association among serum immunoglobulin G (IgG) responses to Aggregatibacter... more Aims: To evaluate the association among serum immunoglobulin G (IgG) responses to Aggregatibacter actinomycetemcomitans (Aa) serotypes a, b and c, Porphyromonas gingivalis (Pg) Tannerella forsythia (Tf) and clinical parameters in Aggressive Periodontitis (AP) subjects. Associations between periodontal pathogens and clinical and immunological parameters were also evaluated. Methods: 38 subjects diagnosed with generalized AP (GAP) and localized AP (LAP) were included. Ten healthy controls were also evaluated. Clinical parameters were assessed and percentages of subgingival levels of Aa, Pg and Tf, (beyond bacterial load), were determined by quantitative real-time polymerase chain reaction. Serum IgG antibody levels against Aa, Pg and Tf were evaluated by enzyme-linked immunosorbent assay.

Research paper thumbnail of Impacto de um diagnóstico periodontal deficiente em pacientes com periodontite agressiva submetidos a tratamento ortodôntico: relato de uma série de casos

Rev Clin Ortodon Dental Press, Jul 1, 2013

aggressive periodontitis under orthodontic treatment: A case series Impacto de um diagnóstico per... more aggressive periodontitis under orthodontic treatment: A case series Impacto de um diagnóstico periodontal deficiente em pacientes com periodontite agressiva submetidos a tratamento ortodôntico: relato de uma série de casos Resumo Abstract Palavras-chave: Periodontite agressiva. Ortodontia. Reabsorção óssea.

Research paper thumbnail of Effect of Periodontal Treatment on Protease-Activated Receptors Expression

Objectives: The objectives of this study were to investigate: 1) whether PAR-1 and PAR-2 mRNA exp... more Objectives: The objectives of this study were to investigate: 1) whether PAR-1 and PAR-2 mRNA expression in the gingival crevicular cells is associated to the clinical severity of chronic periodontitis (CP) and to the mRNA levels of gingipain, 2) the effect of periodontal treatment on PAR-1 and PAR-2 mRNA expression in human chronic periodontitis. Methods: Moderate and severe CP patients and control patients (aged between 33 and 53 years old) were recruited according to inclusion and exclusion criteria. Clinical evaluation consisted of measuring probing depth (PD), clinical attachment loss (CAL), bleeding on probing (BOP), gingival (GI) and plaque index (PI). Gingival crevicular fluid (GCF) samples and clinical parameters were collected before and 6 weeks after non-surgical periodontal treatment. Gingipain, PAR-1 and PAR-2 expression were assessed by q-PCR analysis. Results: CP patients presented increased gingipain and PAR-2 expression at the GCF cells compared to control patients ...

Research paper thumbnail of Periodontal protease-activated receptor 2 expression in diabetics patients

Objectives: Diabetes has been linked to severe periodontal disease. Protease activated receptor 2... more Objectives: Diabetes has been linked to severe periodontal disease. Protease activated receptor 2 (PAR-2) is a G-protein receptor associated with periodontal inflammation. The objective of this study was to investigate PAR-2 mRNA expression at the gingival crevicular fluid (GCF) cells in diabetic patients. Methods: Thirteen periodontally healthy patients with diabetes type 2, and 13 periodontally healthy non-diabetic patients were subjected to periodontal clinical evaluation consisting of measuring probing depths (PD), clinical attachment loss (CAL), and bleeding on probing (BOP). Gingival crevicular fluid (GCF) samples were collected and the PAR-2 expression was assessed by real time polymerase chain reaction (q-PCR). Results: The mean values of PD, CAL, and BOP (%) for the diabetics were not statistically different from the non-diabetics patients. In addition, PAR-2 mRNA levels at the GCF cells for diabetics were not statistically different from non-diabetics patients (p=0.08). Co...

Research paper thumbnail of Influence of Piezosurgery on Bone Healing around Titanium Implants: A Histological Study in Rats

Brazilian Dental Journal, 2016

The aim of this study was to evaluate histomorphometrically the influence of two techniques of de... more The aim of this study was to evaluate histomorphometrically the influence of two techniques of dental implant site preparation on bone healing around titanium implants. Fifteen male Wistar rats (±300 g) were used in the study. Each tibia was randomly assigned to receive the implant site preparation either with a conventional drilling technique (control - DRILL group) or with a piezoelectric device (PIEZO group). The animals were sacrificed after 30 days and then the following histomorphometric parameters were evaluated (percentage) separately for cortical and cancellous regions: proportion of mineralized tissue (PMT) adjacent to implant threads (500 μm adjacent); bone area within the threads (BA) and bone-implant contact (BIC). The results demonstrated that there were no statistically significant differences between both groups for cancellous BIC (p>0.05) and cortical PMT (p>0.05). On the other hand, a higher percentage of BA was observed in the PIEZO group in the cortical (71...

Research paper thumbnail of Oral and Fecal Microbiome in Molar-Incisor Pattern Periodontitis

Frontiers in Cellular and Infection Microbiology

Research paper thumbnail of Impacto da periodontite agressiva e da periodontite crônica na qualidade de vida relacionada à saúde bucal

Brazilian Journal of Implantology and Health Sciences

O objetivo deste estudo transversal foi investigar o efeito de diferentes formas de doenças perio... more O objetivo deste estudo transversal foi investigar o efeito de diferentes formas de doenças periodontais na qualidade de vida relacionada à saúde bucal (OHRQoL). Foram incluídos 52 pacientes com Periodontite Agressiva (AP) ou Periodontite Crônica (PC): nove pacientes com Periodontite Agressiva Localizada (LAP), trinta e três pacientes com Periodontite Agressiva Generalizada (GAP) e dez pacientes com Periodontite Crônica Generalizada (GCP) . Os questionários de Perfil de Impacto na Saúde Oral (OHIP-14) foram distribuídos após um exame clínico que mediu os seguintes parâmetros periodontais: perda dentária, sangramento na sondagem (BoP), profundidade da sondagem (DP), recessão gengival (REC) e nível de inserção clínica (CAL) ) As médias globais da pontuação do OHIP-14 foram 10,6 para o LAP, 16,5 para o GAP e 17,5 para o GCP. Diferença estatisticamente significante (p <0. 01) foi observado entre o grupo LAP e os outros dois grupos. Houve significativamente menos sangramento e recessã...

Research paper thumbnail of Chemokines and cytokines profile in whole saliva of patients with periodontitis

Research paper thumbnail of Effect of periodontal treatment on Aggregatibacter actinomycetemcomitans (Aa) colonization and serum IgG levels against Aa serotypes and Omp29 of aggressive periodontitis patients

Research paper thumbnail of A systematic review and meta-analysis of the survival rate of implants placed in previously failed sites

Brazilian oral research, 2018

The aim of this study was to conduct a systematic review and meta-analysis to assess the clinical... more The aim of this study was to conduct a systematic review and meta-analysis to assess the clinical outcomes of dental implants placed in previously early and late implant failed sites. An electronic literature search was conducted in several databases for articles published up to February 2018. Human clinical trials that received at least one implant in a previously failed site were included. Hence, the PICO question that was aimed to be addressed was: Do patients undergoing implant replacement (second and third attempts) in previous failed sites have survival rates similar to implants placed at first attempts? A random effects model was used to calculate survival weighted means and corresponding 95% Confidence Intervals (CI) among studies. Eleven studies of low to moderate methodological quality were included in this review. Implants placed in sites with history of one and two implant failures had a weighted survival rate (SR) of 88.7% (95%CI 81.7-93.3) and 67.1% (95%CI 51.1-79.9), ...

Research paper thumbnail of The ideal time of systemic metronidazole and amoxicillin administration in the treatment of severe periodontitis: study protocol for a randomized controlled trial

Trials, Jan 27, 2018

The combination of systemic metronidazole (MTZ) and amoxicillin (AMX) with scaling and root plani... more The combination of systemic metronidazole (MTZ) and amoxicillin (AMX) with scaling and root planing (SRP) has shown to be an effective periodontal treatment. However, some essential issues associated with the use of these antibiotics remain unanswered, such as the ideal time of administration during the course of periodontal treatment. Although these agents are often prescribed after the healing phase of the SRP procedure, there is biological plausibility to support its use in conjunction with the mechanical treatment. However, to date, no placebo controlled randomized clinical trial (RCT) has directly compared these two protocols. Therefore, the aim of this RCT is to compare the clinical, microbiological and immunological effects of the adjunctive systemic MTZ + AMX administered in different phases of the treatment of severe periodontitis. Subjects with severe periodontitis (n = 180) are being randomly assigned into three groups (n = 60/group): (i) SRP-only (control group), SRP in ...

Research paper thumbnail of Impact of aggressive periodontitis and chronic periodontitis on oral health-related quality of life

Brazilian oral research, 2018

The purpose of this cross-sectional study was to investigate the effect of different forms of per... more The purpose of this cross-sectional study was to investigate the effect of different forms of periodontal diseases on Oral Health-Related Quality of Life (OHRQoL). Fifty-two patients with Aggressive Periodontitis (AP) or Chronic Periodontitis (CP) were included: nine patients with Localized Aggressive Periodontitis (LAP), thirty-three patients with Generalized Aggressive Periodontitis (GAP) and ten patients with Generalized Chronic Periodontitis (GCP). Oral Health Impact Profile questionnaires (OHIP-14) were distributed after a clinical examination that measured the following periodontal parameters: tooth loss, bleeding on probing (BoP), probing depth (PD), gingival recession (REC) and clinical attachment level (CAL). The global OHIP-14 score means were 10.6 for LAP, 16.5 for GAP, and 17.5 for GCP. A statistically significant difference (p < 0.01) was observed between the LAP group and the other two groups. There was significantly less bleeding and recession in the LAP group than...

Research paper thumbnail of PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity

Brazilian Oral Research, 2017

Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association be... more Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

Research paper thumbnail of Effects of periodontal treatment on primary sjȫgren’s syndrome symptoms

Brazilian Oral Research, 2017

The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatme... more The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (ESSDAI) and subjective (ESSPRI) indexes in patients with primary Sjögren's Syndrome (pSS). Twenty-eight female patients were divided into four groups: pSS patients with or without chronic periodontitis (SCP, SC, respectively), and systemically healthy patients with or without chronic periodontitis (CP, C, respectively). Periodontal clinical examination and immunological and microbiological sample collection were performed at baseline, 30 and 90 days after nonsurgical periodontal treatment (NSPT). Levels of interleukin IL-1β, IL-8 and IL-10 in saliva and gingival crevicular fluid (GCF) were evaluated by ELISA, as well as the expression of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans, (Aa) Tannerella forsythia (Tf), and Treponema denticola (Td), by qPCR. Systemic activity and pSS symptoms were evaluated by ESSDAI and ESSPRI. NSPT resulted in improved periodontal clinical parameters in both SCP and CP groups (p>0.05). Pg, Aa, and Tf levels decreased after NSPT only in CP patients (p<0.05). Significantly greater levels of IL-10 in GCF were verified in both SCP and CP groups (p<0.05). SCP patients showed increased salivary flow rates and decreased ESSPRI scores after NSPT. In conclusion, NSPT in pSS patients resulted in improved clinical and immunological parameters, with no significant effects on microbiological status. pSS patients also showed increased salivary flow and lower ESSPRI scores after therapy. Therefore, it can be suggested that NSPT may improve the quality of life of pSS patients.

Research paper thumbnail of Perfil De Morbidade Dos Usuários Da Divisão De Assistência a Saúde Do Departamento De Assistência Ao Servidor Da PRH/Ufrn

Fiep Bulletin on Line, 2009

Research paper thumbnail of Diagnóstico clínico e complementar das doenças periimplantares

Periodontia, 2008

Base de dados : LILACS. Pesquisa : 544277 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 544277 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 544277. ...

Research paper thumbnail of Gingival Overgrowth Among Renal Transplant Patients Medicated with Cyclosporin and Tacrolimus: Prospective Study

Research paper thumbnail of Incidence of tacrolimus-induced gingival overgrowth in the absence of calcium channel blockers: a short-term study

Journal of Clinical Periodontology, 2007

The aim of this study was to determine the incidence and severity of gingival overgrowth (GO) ind... more The aim of this study was to determine the incidence and severity of gingival overgrowth (GO) induced by tacrolimus (Tcr) compared with ciclosporin A (CiA) in the absence of calcium channel blockers (CCB) in renal transplant recipients. Methods: Forty patients (20 Tcr and 20 CiA) were evaluated before and 30 and 90 days after kidney transplantation. Demographic (age, gender) and periodontal parameters were recorded for all patients. Patients taking CCB at any time during the study were excluded from the investigation. Results: The mean GO score was significantly lower (p 5 0.014) in the Tcr group (6.4%) compared with the CiA group (17.9%) after 90 days of immunosuppressive therapy. At 90 days post-transplant, clinically significant GO was observed in four patients of the CiA group and in two of the Tcr group. This difference was not statistically significant (0.66). Conclusion: No significant difference in the incidence of clinically significant GO was observed between the CiA and Tcr groups up to 90 days of immunosuppressive therapy.

Research paper thumbnail of Orthodontic treatment in patients with aggressive periodontitis

Introduction: Aggressive periodontitis (AP) is a condition that promotes breakdown of the periodo... more Introduction: Aggressive periodontitis (AP) is a condition that promotes breakdown of the periodontal tissues in a short time. In severe cases, pathologic migration of teeth and tooth loss can occur, producing esthetic and functional problems for the patient. Orthodontic treatment may be recommended to restore esthetics and masticatory function. We assessed the effects of orthodontic movement in the periodontal tissues of treated patients with AP. Methods: Ten subjects (ages 25.0 6 5.22 years) with AP received periodontal treatment followed by orthodontic treatment. Maintenance sessions were performed monthly under a strict dental biofilm control. They were compared with 10 periodontally healthy subjects (ages 22.9 6 5.23 years) who received orthodontic treatment. Probing pocket depth, clinical attachment level, bleeding on probing, and dental plaque index were measured at baseline, after orthodontic treatment, and after 4 months. Results: Statistical analysis showed improvement in ...

Research paper thumbnail of Influence of Piezosurgery on Bone Healing around Titanium Implants: A Histological Study in Rats

Brazilian Dental Journal, 2016

The aim of this study was to evaluate histomorphometrically the influence of two techniques of de... more The aim of this study was to evaluate histomorphometrically the influence of two techniques of dental implant site preparation on bone healing around titanium implants. Fifteen male Wistar rats (±300 g) were used in the study. Each tibia was randomly assigned to receive the implant site preparation either with a conventional drilling technique (control - DRILL group) or with a piezoelectric device (PIEZO group). The animals were sacrificed after 30 days and then the following histomorphometric parameters were evaluated (percentage) separately for cortical and cancellous regions: proportion of mineralized tissue (PMT) adjacent to implant threads (500 μm adjacent); bone area within the threads (BA) and bone-implant contact (BIC). The results demonstrated that there were no statistically significant differences between both groups for cancellous BIC (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05) and cortical PMT (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). On the other hand, a higher percentage of BA was observed in the PIEZO group in the cortical (71.50±6.91 and 78.28±4.38 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05) and cancellous regions (9.62±4.06 and 19.94±14.18 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). The piezosurgery also showed higher PMT values in the cancellous zone (9.35±5.54 and 18.72±13.21 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). However, the DRILL group presented better results for BIC in cortical region (80.42±10.88 and 70.25±16.93 for DRILL and PIEZO groups, respectively; p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). In conclusion, for the implant site preparation, the piezosurgery was beneficial to bone healing rates in the cancellous bone region, while the drill technique produced better results in the cortical bone.

Research paper thumbnail of IgG sera levels against a subset of periodontopathogens and severity of disease in aggressive periodontitis patients: A cross-sectional study of selected pocket sites

Aims: To evaluate the association among serum immunoglobulin G (IgG) responses to Aggregatibacter... more Aims: To evaluate the association among serum immunoglobulin G (IgG) responses to Aggregatibacter actinomycetemcomitans (Aa) serotypes a, b and c, Porphyromonas gingivalis (Pg) Tannerella forsythia (Tf) and clinical parameters in Aggressive Periodontitis (AP) subjects. Associations between periodontal pathogens and clinical and immunological parameters were also evaluated. Methods: 38 subjects diagnosed with generalized AP (GAP) and localized AP (LAP) were included. Ten healthy controls were also evaluated. Clinical parameters were assessed and percentages of subgingival levels of Aa, Pg and Tf, (beyond bacterial load), were determined by quantitative real-time polymerase chain reaction. Serum IgG antibody levels against Aa, Pg and Tf were evaluated by enzyme-linked immunosorbent assay.

Research paper thumbnail of Impacto de um diagnóstico periodontal deficiente em pacientes com periodontite agressiva submetidos a tratamento ortodôntico: relato de uma série de casos

Rev Clin Ortodon Dental Press, Jul 1, 2013

aggressive periodontitis under orthodontic treatment: A case series Impacto de um diagnóstico per... more aggressive periodontitis under orthodontic treatment: A case series Impacto de um diagnóstico periodontal deficiente em pacientes com periodontite agressiva submetidos a tratamento ortodôntico: relato de uma série de casos Resumo Abstract Palavras-chave: Periodontite agressiva. Ortodontia. Reabsorção óssea.

Research paper thumbnail of Effect of Periodontal Treatment on Protease-Activated Receptors Expression

Objectives: The objectives of this study were to investigate: 1) whether PAR-1 and PAR-2 mRNA exp... more Objectives: The objectives of this study were to investigate: 1) whether PAR-1 and PAR-2 mRNA expression in the gingival crevicular cells is associated to the clinical severity of chronic periodontitis (CP) and to the mRNA levels of gingipain, 2) the effect of periodontal treatment on PAR-1 and PAR-2 mRNA expression in human chronic periodontitis. Methods: Moderate and severe CP patients and control patients (aged between 33 and 53 years old) were recruited according to inclusion and exclusion criteria. Clinical evaluation consisted of measuring probing depth (PD), clinical attachment loss (CAL), bleeding on probing (BOP), gingival (GI) and plaque index (PI). Gingival crevicular fluid (GCF) samples and clinical parameters were collected before and 6 weeks after non-surgical periodontal treatment. Gingipain, PAR-1 and PAR-2 expression were assessed by q-PCR analysis. Results: CP patients presented increased gingipain and PAR-2 expression at the GCF cells compared to control patients ...

Research paper thumbnail of Periodontal protease-activated receptor 2 expression in diabetics patients

Objectives: Diabetes has been linked to severe periodontal disease. Protease activated receptor 2... more Objectives: Diabetes has been linked to severe periodontal disease. Protease activated receptor 2 (PAR-2) is a G-protein receptor associated with periodontal inflammation. The objective of this study was to investigate PAR-2 mRNA expression at the gingival crevicular fluid (GCF) cells in diabetic patients. Methods: Thirteen periodontally healthy patients with diabetes type 2, and 13 periodontally healthy non-diabetic patients were subjected to periodontal clinical evaluation consisting of measuring probing depths (PD), clinical attachment loss (CAL), and bleeding on probing (BOP). Gingival crevicular fluid (GCF) samples were collected and the PAR-2 expression was assessed by real time polymerase chain reaction (q-PCR). Results: The mean values of PD, CAL, and BOP (%) for the diabetics were not statistically different from the non-diabetics patients. In addition, PAR-2 mRNA levels at the GCF cells for diabetics were not statistically different from non-diabetics patients (p=0.08). Co...