Paulo Serafini | Universidade de São Paulo (original) (raw)

Papers by Paulo Serafini

Research paper thumbnail of Influence of vitrification on mouse metaphase II oocyte spindle dynamics and chromatin alignment

Objective: To evaluate influences of vitrification and warming of metaphase II (MII) mouse oocyte... more Objective: To evaluate influences of vitrification and warming of metaphase II (MII) mouse oocytes on survival, spindle dynamics, spindle morphology, and chromatin alignment on metaphase plates. Design: Experimental animal study. Setting: University animal laboratory. Animal(s): Eight-week-old B6D2F1 mice. Intervention(s): Denuded MII oocytes were used fresh (control), exposed to vitrification/warming solutions (Sol Expos), or vitrified and warmed (Vitr). Main Outcome Measure(s): Oocyte recovery and survival after warming and the influence of solution exposure and cryopreservation on spindle dynamics and chromatin alignment. Result(s): Cryopreservation of two or 10 oocytes per straw resulted in recovery (100% AE 0% and 95% AE 4%, respectively; mean AE SE) and survival (95% AE 2% and 98% AE 2%, respectively). Immediately after warming (Vitr), significantly fewer oocytes assessed with immunocytochemistry contained spindles, compared with control and Sol Expos. When oocytes were placed into a 37 C environment for 2 hours after exposure or warming, the ability to recognize spindles by immunocytochemistry was not significantly different between groups. Using live-cell time-lapse imaging with LC-Polscope, similar time-dependent spindle formation dynamics were observed. At 2 hours after collection or treatment, spindle morphology and length were not significantly different between the groups, nor was the incidence of aberrant alignment of chromatin on metaphase plates. Conclusion(s): Immediately after warming of vitrified MII oocytes, b-tubulin is depolymerized and chromatin remains condensed on the metaphase plate. Within a 2-hour period, b-tubulin repolymerizes, forming morphologically normal metaphase spindles with properly aligned chromatin.

Research paper thumbnail of Resistance and pulsatility indexes of uterine arteries in infertile patients

International Journal of Gynecology & Obstetrics, 2000

Research paper thumbnail of Should we treat patients for poor response following ovulation induction with low doses of recombinant FSH?

Fertility and Sterility, 2010

may be used (instead of HCG) to trigger ovulation while mitigating the risk of ovarian hyperstimu... more may be used (instead of HCG) to trigger ovulation while mitigating the risk of ovarian hyperstimulation in this group. In this study, we analyzed results of vitrified/warmed oocytes obtained from donors in Antagonist cycles triggered either by hCG or by Lupron.

Research paper thumbnail of Laparoscopic Excision of Infiltrative Endometriosis Improves Outcomes of Women Previously Treated with In Vitro Fertilization

Journal of Minimally Invasive Gynecology, 2008

Research paper thumbnail of Extensive resection of deep infiltrative endometriosis significantly increases spontaneous pregnancy rates

Fertility and Sterility, 2008

OBJECTIVE: To compare clinical outcomes and hospital costs of myomectomy via da Vinci Robot versu... more OBJECTIVE: To compare clinical outcomes and hospital costs of myomectomy via da Vinci Robot versus traditional laparotomy.

Research paper thumbnail of Extensive Excision of Deep Infiltrative Endometriosis before In Vitro Fertilization Significantly Improves Pregnancy Rates

Journal of Minimally Invasive Gynecology, 2008

Research paper thumbnail of Does radical laparoscopy (RLSC) for deep invasive endometriosis (DIE) in infertile women increase outcome of IVF? results of a prospective study

Fertility and Sterility, 2007

Research paper thumbnail of 296: Rectosigmoid Endometriosis Discoid Excision Using the Circular Stapler: Report of 9 Consecutive Cases

Journal of Minimally Invasive Gynecology, 2007

ries, the adhesion scores in the Pro/GEL-AB treated group showed a statistically significant impr... more ries, the adhesion scores in the Pro/GEL-AB treated group showed a statistically significant improvement in adhesion reduction (p Ͻ 0.05, Chi-squared or Tukey-Kramer) relative to the control. Conclusion: The results of this study showed Pro/GEL-AB spray significantly reduced adhesion formation relative to an untreated surgical control in both sidewall and cecal models.

Research paper thumbnail of An alternate approach to ovulation induction (OI) for IVF: Daily late follicular phase administration of 200 IU hCG − Results of prospective, randomized trial

Fertility and Sterility, 2004

Research paper thumbnail of Systematic review of cell adhesion molecules and estrogen receptor expression in the endometrium of patients with polycystic ovary syndrome

International Journal of Gynecology & Obstetrics, 2014

Infertility associated with polycystic ovary syndrome (PCOS) could be related to many mechanisms ... more Infertility associated with polycystic ovary syndrome (PCOS) could be related to many mechanisms including endometrial factors. To review cell adhesion molecule and estrogen receptor expression in the endometrium. A systematic review was performed of the Medline and Cochrane databases for papers published in any language between 2004 and 2014. The search term was "'polycystic ovary syndrome' OR 'Stein Leventhal syndrome' OR 'anovulation' AND 'endometrium' OR 'endometria.'" SELECTION CRITERIA: Research studies on endometrial cell adhesion molecules and estrogen receptor expression among women with PCOS diagnosed according to the Rotterdam criteria were included. Data were extracted from identified studies and the quality of assessment was analyzed. Six studies were included. Data were controversial with respect to MUC1 and αVβ3 integrin expression with significantly higher and lower levels, respectively, in women with PCOS. Estrogen receptor expression was enhanced among patients with PCOS as compared with healthy women. Endometrial factors influence embryo receptivity as indicated by the molecular mediators identified in the studies, including cell adhesion molecules and the estrogen receptor.

Research paper thumbnail of Laboratory methods in the study of endometrial Claudin-4

Methods in molecular biology (Clifton, N.J.), 2011

Immunohistochemistry is a suitable method for the detection of proteins from the Claudin family a... more Immunohistochemistry is a suitable method for the detection of proteins from the Claudin family and several antibodies are commercially available for the detection of Claudin congeners. Immunodetection of Caludin-4 in the paraffin-embedded specimens might be a useful tool for studying the role of these proteins in the cyclic transformation of the endometrium and its role in the endometrial receptivity; furthermore, other components of the junctional zone involved in the transformational process of the endometrium can be detected by means of immunohistochemistry/immunofluorescence with several polyclonal or monoclonal antibodies. The aim of this chapter is to comprehensively overview the materials and methods to perform the endometrial biopsy and to detect Claudin-4 in paraffin-embedded samples of endometrium. Additionally, the interpretation of the results is addressed.

Research paper thumbnail of The Relationship Among HOXA10, Estrogen Receptor α, Progesterone Receptor, and Progesterone Receptor B Proteins in Rectosigmoid Endometriosis: A Tissue Microarray Study

Reproductive sciences (Thousand Oaks, Calif.), 2015

Very few studies have evaluated the expression of homeobox A10 (HOXA10) and steroid (estrogen and... more Very few studies have evaluated the expression of homeobox A10 (HOXA10) and steroid (estrogen and progesterone) receptors exclusively in deep endometriosis. Conclusions drawn from studies evaluating peritoneal and ovarian endometriosis are usually generalized to explain the pathogenesis of the disease as a whole. We aimed to evaluate the expression of HOXA10, estrogen receptor α (ER-α), progesterone receptor (PR), and PR-B in rectosigmoid endometriosis (RE), a typical model of deep disease. We used RE samples from 18 consecutive patients to construct tissue microarray blocks. Nine patients each were operated during the proliferative and secretory phases of the menstrual cycle. We quantified the expressions of proteins by immunohistochemistry using the modified Allred score. The HOXA10 was expressed in the stroma of nodules during the secretory phase in 5 of the 18 patients. Expression of ER-α (in 16 of 18 patients), PR (in 17 of 18 patients), and PR-B (17 of 18 patients) was moderat...

Research paper thumbnail of Laparoscopy with extensive excision of deep infiltrative endometriosis before in vitro fertilization significantly increases the likelihood of pregnancy

Fertility and Sterility, 2008

Research paper thumbnail of Serum Levels of Antimullerian Hormone Appear To Be Unaffected by the Presence of Deep-Infiltrating Endometriosis Associated to Endometriomas

Journal of Minimally Invasive Gynecology, 2010

Research paper thumbnail of Administration of a pharmacophysiologic dose of recombinant human chorionic gonadotropin at menses promotes corpus luteum rescue

International Journal of Gynecology & Obstetrics, 2010

Research paper thumbnail of Tests for ovarian reserve: reliability and utility

Current Opinion in Obstetrics & Gynecology, 2010

This review discusses ovarian reserve tests for ovulation induction and their application in dete... more This review discusses ovarian reserve tests for ovulation induction and their application in determining fertility capacity, and their current applications to assess risk of natural ovarian failure and to estimate ovarian function after cancer treatment. The current arsenal of ovarian reserve tests comprises hormonal markers [basal follicle stimulating hormone, estradiol, inhibin-B, antimullerian hormone (AMH)] and ultrasonographic markers [ovarian volume, antral follicle counts (AFCs)]. These markers have limitations in terms of which test(s) should be used to reliably predict ovarian reserve with regard to accuracy, invasiveness, cost, convenience, and utility. Several studies have correlated sonographic AFCs with serum AMH levels for predicting the ovarian response to ovulation induction protocols during assisted reproduction treatments. Serum AMH levels and AFC are reliable tests for predicting the ovarian response to ovulation induction. However, none of the currently employed tests of ovarian reserve can reliably predict pregnancy after assisted conception. Further, ovarian reserve tests cannot predict the onset of reproductive and hormonal menopause; thus, they should be used with caution for reproductive life-programming counseling. Moreover, there is no evidence to support the use of ovarian reserve tests to estimate the risk of ovarian sufficiency after cancer treatments.

Research paper thumbnail of Endometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization

International Journal of Gynecology & Obstetrics, 2008

ObjectiveTo determine whether there is an association between endometrial expression of leukemia ... more ObjectiveTo determine whether there is an association between endometrial expression of leukemia inhibitory factor (LIF) in the luteal phase of the menstrual cycle preceding in vitro fertilization (IVF) and treatment outcome.

Research paper thumbnail of Simple pronuclear analysis: relation to embryo cleavage on day 3

Fertility and Sterility, 2007

TABLE 1. Treatment of mild/moderate male factor infertility with conventional IVF Non-Male Factor... more TABLE 1. Treatment of mild/moderate male factor infertility with conventional IVF Non-Male Factor (IVF) Mild/Moderate Male Factor (IVF) Severe Male Factor (ICSI) Positive bhCG/Retrievals 67.9% (163/240) 68.5% (159/232) 67.5% (27/40) Fetal Cardiac Activity/Retrievals 57.9% (139/240) 59.1% (137/232) 55.0% (22/40) Live Births/Retrievals 55.0% (132/240) 56.4% (131/232) 52.5% (21/40) % Implantations 47.9% (196/409) 50.5% (201/398) 36.1% (26/72)

Research paper thumbnail of P-5

Fertility and Sterility, 2006

Research paper thumbnail of Total dose of cetrorelix GnRH antagonist does not affect pregnancy rates in patients undergoing in vitro fertilization at recombinant FSH ovarian stimulation regimen

Fertility and Sterility, 2008

MATERIALS AND METHODS: A total of 2467 embryos from couples undergoing IVF/ICSI in our SC and OD ... more MATERIALS AND METHODS: A total of 2467 embryos from couples undergoing IVF/ICSI in our SC and OD program were evaluated for BR. Human blastocysts were scored on day 5 according to the formation of the blastocoel's cavity. Donors and patients with PCO or endometriosis were excluded. Estradiol levels were measured in donors and patients the day of hCG administration and threshold point of 2000 pg/ml were considered form our calculations. An stratified test for cohorts was used to compare BR in those coming HE and not, taking oocyte origin (OD or SC) like confusion factor. RESULTS: BR is clearly improved OR¼1.86 (CI95 1.57-2.19) and HE are less present OR¼0.39 (CI95 0.33-0.46) in OD compared with SC. 120h after fertilization Arrested Morula Blastocyst PR p value S478

Research paper thumbnail of Influence of vitrification on mouse metaphase II oocyte spindle dynamics and chromatin alignment

Objective: To evaluate influences of vitrification and warming of metaphase II (MII) mouse oocyte... more Objective: To evaluate influences of vitrification and warming of metaphase II (MII) mouse oocytes on survival, spindle dynamics, spindle morphology, and chromatin alignment on metaphase plates. Design: Experimental animal study. Setting: University animal laboratory. Animal(s): Eight-week-old B6D2F1 mice. Intervention(s): Denuded MII oocytes were used fresh (control), exposed to vitrification/warming solutions (Sol Expos), or vitrified and warmed (Vitr). Main Outcome Measure(s): Oocyte recovery and survival after warming and the influence of solution exposure and cryopreservation on spindle dynamics and chromatin alignment. Result(s): Cryopreservation of two or 10 oocytes per straw resulted in recovery (100% AE 0% and 95% AE 4%, respectively; mean AE SE) and survival (95% AE 2% and 98% AE 2%, respectively). Immediately after warming (Vitr), significantly fewer oocytes assessed with immunocytochemistry contained spindles, compared with control and Sol Expos. When oocytes were placed into a 37 C environment for 2 hours after exposure or warming, the ability to recognize spindles by immunocytochemistry was not significantly different between groups. Using live-cell time-lapse imaging with LC-Polscope, similar time-dependent spindle formation dynamics were observed. At 2 hours after collection or treatment, spindle morphology and length were not significantly different between the groups, nor was the incidence of aberrant alignment of chromatin on metaphase plates. Conclusion(s): Immediately after warming of vitrified MII oocytes, b-tubulin is depolymerized and chromatin remains condensed on the metaphase plate. Within a 2-hour period, b-tubulin repolymerizes, forming morphologically normal metaphase spindles with properly aligned chromatin.

Research paper thumbnail of Resistance and pulsatility indexes of uterine arteries in infertile patients

International Journal of Gynecology & Obstetrics, 2000

Research paper thumbnail of Should we treat patients for poor response following ovulation induction with low doses of recombinant FSH?

Fertility and Sterility, 2010

may be used (instead of HCG) to trigger ovulation while mitigating the risk of ovarian hyperstimu... more may be used (instead of HCG) to trigger ovulation while mitigating the risk of ovarian hyperstimulation in this group. In this study, we analyzed results of vitrified/warmed oocytes obtained from donors in Antagonist cycles triggered either by hCG or by Lupron.

Research paper thumbnail of Laparoscopic Excision of Infiltrative Endometriosis Improves Outcomes of Women Previously Treated with In Vitro Fertilization

Journal of Minimally Invasive Gynecology, 2008

Research paper thumbnail of Extensive resection of deep infiltrative endometriosis significantly increases spontaneous pregnancy rates

Fertility and Sterility, 2008

OBJECTIVE: To compare clinical outcomes and hospital costs of myomectomy via da Vinci Robot versu... more OBJECTIVE: To compare clinical outcomes and hospital costs of myomectomy via da Vinci Robot versus traditional laparotomy.

Research paper thumbnail of Extensive Excision of Deep Infiltrative Endometriosis before In Vitro Fertilization Significantly Improves Pregnancy Rates

Journal of Minimally Invasive Gynecology, 2008

Research paper thumbnail of Does radical laparoscopy (RLSC) for deep invasive endometriosis (DIE) in infertile women increase outcome of IVF? results of a prospective study

Fertility and Sterility, 2007

Research paper thumbnail of 296: Rectosigmoid Endometriosis Discoid Excision Using the Circular Stapler: Report of 9 Consecutive Cases

Journal of Minimally Invasive Gynecology, 2007

ries, the adhesion scores in the Pro/GEL-AB treated group showed a statistically significant impr... more ries, the adhesion scores in the Pro/GEL-AB treated group showed a statistically significant improvement in adhesion reduction (p Ͻ 0.05, Chi-squared or Tukey-Kramer) relative to the control. Conclusion: The results of this study showed Pro/GEL-AB spray significantly reduced adhesion formation relative to an untreated surgical control in both sidewall and cecal models.

Research paper thumbnail of An alternate approach to ovulation induction (OI) for IVF: Daily late follicular phase administration of 200 IU hCG − Results of prospective, randomized trial

Fertility and Sterility, 2004

Research paper thumbnail of Systematic review of cell adhesion molecules and estrogen receptor expression in the endometrium of patients with polycystic ovary syndrome

International Journal of Gynecology & Obstetrics, 2014

Infertility associated with polycystic ovary syndrome (PCOS) could be related to many mechanisms ... more Infertility associated with polycystic ovary syndrome (PCOS) could be related to many mechanisms including endometrial factors. To review cell adhesion molecule and estrogen receptor expression in the endometrium. A systematic review was performed of the Medline and Cochrane databases for papers published in any language between 2004 and 2014. The search term was "'polycystic ovary syndrome' OR 'Stein Leventhal syndrome' OR 'anovulation' AND 'endometrium' OR 'endometria.'" SELECTION CRITERIA: Research studies on endometrial cell adhesion molecules and estrogen receptor expression among women with PCOS diagnosed according to the Rotterdam criteria were included. Data were extracted from identified studies and the quality of assessment was analyzed. Six studies were included. Data were controversial with respect to MUC1 and αVβ3 integrin expression with significantly higher and lower levels, respectively, in women with PCOS. Estrogen receptor expression was enhanced among patients with PCOS as compared with healthy women. Endometrial factors influence embryo receptivity as indicated by the molecular mediators identified in the studies, including cell adhesion molecules and the estrogen receptor.

Research paper thumbnail of Laboratory methods in the study of endometrial Claudin-4

Methods in molecular biology (Clifton, N.J.), 2011

Immunohistochemistry is a suitable method for the detection of proteins from the Claudin family a... more Immunohistochemistry is a suitable method for the detection of proteins from the Claudin family and several antibodies are commercially available for the detection of Claudin congeners. Immunodetection of Caludin-4 in the paraffin-embedded specimens might be a useful tool for studying the role of these proteins in the cyclic transformation of the endometrium and its role in the endometrial receptivity; furthermore, other components of the junctional zone involved in the transformational process of the endometrium can be detected by means of immunohistochemistry/immunofluorescence with several polyclonal or monoclonal antibodies. The aim of this chapter is to comprehensively overview the materials and methods to perform the endometrial biopsy and to detect Claudin-4 in paraffin-embedded samples of endometrium. Additionally, the interpretation of the results is addressed.

Research paper thumbnail of The Relationship Among HOXA10, Estrogen Receptor α, Progesterone Receptor, and Progesterone Receptor B Proteins in Rectosigmoid Endometriosis: A Tissue Microarray Study

Reproductive sciences (Thousand Oaks, Calif.), 2015

Very few studies have evaluated the expression of homeobox A10 (HOXA10) and steroid (estrogen and... more Very few studies have evaluated the expression of homeobox A10 (HOXA10) and steroid (estrogen and progesterone) receptors exclusively in deep endometriosis. Conclusions drawn from studies evaluating peritoneal and ovarian endometriosis are usually generalized to explain the pathogenesis of the disease as a whole. We aimed to evaluate the expression of HOXA10, estrogen receptor α (ER-α), progesterone receptor (PR), and PR-B in rectosigmoid endometriosis (RE), a typical model of deep disease. We used RE samples from 18 consecutive patients to construct tissue microarray blocks. Nine patients each were operated during the proliferative and secretory phases of the menstrual cycle. We quantified the expressions of proteins by immunohistochemistry using the modified Allred score. The HOXA10 was expressed in the stroma of nodules during the secretory phase in 5 of the 18 patients. Expression of ER-α (in 16 of 18 patients), PR (in 17 of 18 patients), and PR-B (17 of 18 patients) was moderat...

Research paper thumbnail of Laparoscopy with extensive excision of deep infiltrative endometriosis before in vitro fertilization significantly increases the likelihood of pregnancy

Fertility and Sterility, 2008

Research paper thumbnail of Serum Levels of Antimullerian Hormone Appear To Be Unaffected by the Presence of Deep-Infiltrating Endometriosis Associated to Endometriomas

Journal of Minimally Invasive Gynecology, 2010

Research paper thumbnail of Administration of a pharmacophysiologic dose of recombinant human chorionic gonadotropin at menses promotes corpus luteum rescue

International Journal of Gynecology & Obstetrics, 2010

Research paper thumbnail of Tests for ovarian reserve: reliability and utility

Current Opinion in Obstetrics & Gynecology, 2010

This review discusses ovarian reserve tests for ovulation induction and their application in dete... more This review discusses ovarian reserve tests for ovulation induction and their application in determining fertility capacity, and their current applications to assess risk of natural ovarian failure and to estimate ovarian function after cancer treatment. The current arsenal of ovarian reserve tests comprises hormonal markers [basal follicle stimulating hormone, estradiol, inhibin-B, antimullerian hormone (AMH)] and ultrasonographic markers [ovarian volume, antral follicle counts (AFCs)]. These markers have limitations in terms of which test(s) should be used to reliably predict ovarian reserve with regard to accuracy, invasiveness, cost, convenience, and utility. Several studies have correlated sonographic AFCs with serum AMH levels for predicting the ovarian response to ovulation induction protocols during assisted reproduction treatments. Serum AMH levels and AFC are reliable tests for predicting the ovarian response to ovulation induction. However, none of the currently employed tests of ovarian reserve can reliably predict pregnancy after assisted conception. Further, ovarian reserve tests cannot predict the onset of reproductive and hormonal menopause; thus, they should be used with caution for reproductive life-programming counseling. Moreover, there is no evidence to support the use of ovarian reserve tests to estimate the risk of ovarian sufficiency after cancer treatments.

Research paper thumbnail of Endometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization

International Journal of Gynecology & Obstetrics, 2008

ObjectiveTo determine whether there is an association between endometrial expression of leukemia ... more ObjectiveTo determine whether there is an association between endometrial expression of leukemia inhibitory factor (LIF) in the luteal phase of the menstrual cycle preceding in vitro fertilization (IVF) and treatment outcome.

Research paper thumbnail of Simple pronuclear analysis: relation to embryo cleavage on day 3

Fertility and Sterility, 2007

TABLE 1. Treatment of mild/moderate male factor infertility with conventional IVF Non-Male Factor... more TABLE 1. Treatment of mild/moderate male factor infertility with conventional IVF Non-Male Factor (IVF) Mild/Moderate Male Factor (IVF) Severe Male Factor (ICSI) Positive bhCG/Retrievals 67.9% (163/240) 68.5% (159/232) 67.5% (27/40) Fetal Cardiac Activity/Retrievals 57.9% (139/240) 59.1% (137/232) 55.0% (22/40) Live Births/Retrievals 55.0% (132/240) 56.4% (131/232) 52.5% (21/40) % Implantations 47.9% (196/409) 50.5% (201/398) 36.1% (26/72)

Research paper thumbnail of P-5

Fertility and Sterility, 2006

Research paper thumbnail of Total dose of cetrorelix GnRH antagonist does not affect pregnancy rates in patients undergoing in vitro fertilization at recombinant FSH ovarian stimulation regimen

Fertility and Sterility, 2008

MATERIALS AND METHODS: A total of 2467 embryos from couples undergoing IVF/ICSI in our SC and OD ... more MATERIALS AND METHODS: A total of 2467 embryos from couples undergoing IVF/ICSI in our SC and OD program were evaluated for BR. Human blastocysts were scored on day 5 according to the formation of the blastocoel's cavity. Donors and patients with PCO or endometriosis were excluded. Estradiol levels were measured in donors and patients the day of hCG administration and threshold point of 2000 pg/ml were considered form our calculations. An stratified test for cohorts was used to compare BR in those coming HE and not, taking oocyte origin (OD or SC) like confusion factor. RESULTS: BR is clearly improved OR¼1.86 (CI95 1.57-2.19) and HE are less present OR¼0.39 (CI95 0.33-0.46) in OD compared with SC. 120h after fertilization Arrested Morula Blastocyst PR p value S478