Veridiana Munford | Universidade de São Paulo (original) (raw)
Papers by Veridiana Munford
Comprehensive Series in Photochemical & Photobiological Sciences, 2021
Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrh... more Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P
Rev Microbiol, Sep 1, 1993
ABSTRACT Duas amostras de fezes de equinos (EQ/28 e EQ/29) foram recebidas para diagnóstico no In... more ABSTRACT Duas amostras de fezes de equinos (EQ/28 e EQ/29) foram recebidas para diagnóstico no Instituto Biológico. As amostras eram provenientes de Orlândia, Säo Paulo, de dois animais com diarréia. A identificaçäo de rotavírus foi feita através de ensaio imunoenzimático (EIARA/FIOCRUZ) e por eletroforese em gel de poliacrilamida (PAGE). As amostras foram positivas para rotavírus pelas duas técnicas, com eletroferótipo característico de rotavírus equino (segmentos 3 e 4 bem próximos) e segmentos 7, 8 e 9 separados). O rotavírus foi isolado em linhagem celular de rim fetal de macaco Rhesus (MA104), com a adiçäo de tripsina, em equipamento do tipo "roller", chegando a sexta passagem, com efeito citopítico característico. Os lisados de cada passagem em culturas celulares foram positivos para rotavírus por EIARA e PAGE, com eletroferótipo semelhante ao da amostra original, em relaçäo aos segmentos 1 a 6, e com algumas diferenças na migraçäo dos segmentos 7 a 11. As amostras originais e lisados das passagens em culturas de células foram testados para determinaçäo de subgrupo, com anticorpos monoclonais (MAb) específicos para grupo A, subgrupo I e subgrupo II, através de um teste imunoenzimático. Tanto as amostras originais quanto os lisados reagiram com o MAb específico para o grupo A e näo reagiram com os anticorpos antisubgrupos I e II, da mesma forma que a amostra H-2 e outros rotavírus equinos. Este é o primeiro isolamento de um rotavírus equino no Brasil, tornando-se importante elucidar sua prevalência em animais normais e com diarréia
Braz J Microbiol, 2007
... Veridiana Munford I ; Eloisa Correia Souza II ; Thabata Alessandra Ramos Caruzo I ; Marina Ba... more ... Veridiana Munford I ; Eloisa Correia Souza II ; Thabata Alessandra Ramos Caruzo I ; Marina Baquerizo ... MAbs against serotypes G1, G2, G3 and G4 (Serotec Rota-MA, Japan) were ... 26. Linhares, AC; Mascarenhas, JDP; Gusmão, RHP; Gabbay, YB; Fialho, AM; Leite, JP (2002). ...
... À Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), pela concessão de bolsa de d... more ... À Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), pela concessão de bolsa de doutorado e pelo apoio financeiro a esta pesquisa. Page 8. CONTINUE A NADAR.... Dory Filme: Procurando Nemo Walt Disney Pictures Page 9. ...
Revista da Sociedade Brasileira de Medicina Tropical, 2007
A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-ye... more A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-year-old children with diarrhea, were used for this study. Molecular characterization of the G and P rotavirus genotypes was performed using the reverse transcriptase polymerase chain reaction. The following results were obtained for the P genotypes: P[8] (17.1%), P[1] (4.9%), P[4] (3.3%), P[6, M37] (2.4%) and mixtures (27.6%). The P[1]+P[8] mixture was found in 19.5% of the samples. For the G genotypes, the results were: G1 (25.2%), G5 (13.8%), G2 (2.5%), G4 (2.5%), G9 (0.8%) and mixtures (41.5%). G1+G5 was the mixture most frequently found (12.1%). Our results showed unusual combinations such as P[1]G5 and P[1]+P[8]G5. The high percentage of mixtures and unusual combinations containing mixtures of human and animal rotavirus genotypes strongly suggests the possibility of gene reassortment and interspecies transmission. RESUMO Um total de 123 amostras fecais de crianças de 0 a 2 anos com diarréia, coletadas em Teresina, Piauí, entre 1994 e 1996 foi utilizada neste estudo. Para a caracterização molecular dos genótipos G e P de rotavírus, foram realizadas as reações de transcriptase reversa e reação em cadeia pela polimerase. Os seguintes resultados foram obtidos para o A alta porcentagem de misturas e as combinações não usuais contendo misturas de genótipos de rotavirus humanos e animais sugerem fortemente a possibilidade de rearranjo genético e transmissão interspecies.
Genetics and Molecular Biology, 2014
The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) b... more The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) but later in many others, led to striking observations on the association of molecular defects and patients' clinical phenotypes. For example, patients with syndromes resulting from defective nucleotide excision repair (NER) or translesion synthesis (TLS) present high levels of skin cancer in areas exposed to sunlight. However, some defects in NER also lead to more severe symptoms, such as developmental and neurological impairment and signs of premature aging. Skin cancer in XP patients is clearly associated with increased mutagenesis and genomic instability, reflecting the defective repair of DNA lesions. By analogy, more severe symptoms observed in NER-defective patients have also been associated with defective repair, likely involving cell death after transcription blockage of damaged templates. Endogenously induced DNA lesions, particularly through oxidative stress, have been identified as responsible for these severe pathologies. However, this association is not that clear and alternative explanations have been proposed. Despite high levels of exposure to intense sunlight, patients from tropical countries receive little attention or care, which likely also reflects the lack of understanding of how DNA damage causes cancer and premature aging.
Memorias Do Instituto Oswaldo Cruz, Dec 1, 2007
The purpose of this study was to characterize astrovirus in faecal samples collected from childre... more The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets: EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) with persistent diarrhea, and 9/55 (16.3%) from control individuals. No data was available for 15/40 (37.5%) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6%) were positive: 12/158 (7.6%) from individuals with acute diarrhea and 6/29 (20.7%) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2%) were characterized as human astrovirus type-1 (HAstV-1), two (3.0%) as HAstV-2, two (3.0%) as HAstV-3, and three (4.5%) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5%) was characterized as HAstV-1, six (33.3%) as HAstV-2, and one (5.5%) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected.
Memórias do Instituto Oswaldo Cruz, 2015
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral struct... more Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for ...
Eur J Clin Microbiol Infect D, 2003
Memórias do Instituto Oswaldo Cruz, 2015
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral struct... more Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
A study was accomplished to determine the occurrence of rotavirus in feces of calves, in the Stat... more A study was accomplished to determine the occurrence of rotavirus in feces of calves, in the State of São Paulo, Brazil. A total of 375 samples of feces from calves 1 to 45 day-old were collected. The animals belonged to farms situated in six counties of the northeast region of the State. One hundred and ninety tree out of these samples belonged to animals with a clinical picture of diarrhea and 182 were obtained from clinically healthy animals. The techniques used for the detection of rotavirus were the enzyme immunoassay (EIA) and the polyacrilamide gel electrophoresis (PAGE). By the use of EIA, 11.2% (42/375) of the samples were positive for rotavirus. Among the samples of diarrheic calves, 15% (29/193) were found to be positive for rotavirus, whereas 7.1 (13/182) positive samples were obtained from clinically healthy animals. The PAGE test presented a lower sensitivity than EIA, since from the 42 positive samples in EIA, only 36 presented an electrophoretical profile characteris...
![Research paper thumbnail of Molecular Characterization of Porcine Rotaviruses from the Southern Region of Brazil: Characterization of an Atypical Genotype G[9] Strain](https://attachments.academia-assets.com/42078894/thumbnails/1.jpg)
](Journal of Clinical Microbiology
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
![Research paper thumbnail of Molecular Characterization of Porcine Rotaviruses from the Southern Region of Brazil: Characterization of an Atypical Genotype G[9] Strain](https://attachments.academia-assets.com/42078888/thumbnails/1.jpg)
](Journal of Clinical Microbiology
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
![Research paper thumbnail of Molecular characterization of porcine rotaviruses from the southern region of Brazil: characterization of an atypical genotype G[9] strain](https://attachments.academia-assets.com/42078891/thumbnails/1.jpg)
](Journal of clinical microbiology, 2000
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1996
Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against ... more Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 micrograms/ml and 500 micrograms/ml. respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 micrograms/ml. Virucidal activity was not observed at 62.5 micrograms/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 micrograms/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Comprehensive Series in Photochemical & Photobiological Sciences, 2021
Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrh... more Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P
Rev Microbiol, Sep 1, 1993
ABSTRACT Duas amostras de fezes de equinos (EQ/28 e EQ/29) foram recebidas para diagnóstico no In... more ABSTRACT Duas amostras de fezes de equinos (EQ/28 e EQ/29) foram recebidas para diagnóstico no Instituto Biológico. As amostras eram provenientes de Orlândia, Säo Paulo, de dois animais com diarréia. A identificaçäo de rotavírus foi feita através de ensaio imunoenzimático (EIARA/FIOCRUZ) e por eletroforese em gel de poliacrilamida (PAGE). As amostras foram positivas para rotavírus pelas duas técnicas, com eletroferótipo característico de rotavírus equino (segmentos 3 e 4 bem próximos) e segmentos 7, 8 e 9 separados). O rotavírus foi isolado em linhagem celular de rim fetal de macaco Rhesus (MA104), com a adiçäo de tripsina, em equipamento do tipo "roller", chegando a sexta passagem, com efeito citopítico característico. Os lisados de cada passagem em culturas celulares foram positivos para rotavírus por EIARA e PAGE, com eletroferótipo semelhante ao da amostra original, em relaçäo aos segmentos 1 a 6, e com algumas diferenças na migraçäo dos segmentos 7 a 11. As amostras originais e lisados das passagens em culturas de células foram testados para determinaçäo de subgrupo, com anticorpos monoclonais (MAb) específicos para grupo A, subgrupo I e subgrupo II, através de um teste imunoenzimático. Tanto as amostras originais quanto os lisados reagiram com o MAb específico para o grupo A e näo reagiram com os anticorpos antisubgrupos I e II, da mesma forma que a amostra H-2 e outros rotavírus equinos. Este é o primeiro isolamento de um rotavírus equino no Brasil, tornando-se importante elucidar sua prevalência em animais normais e com diarréia
Braz J Microbiol, 2007
... Veridiana Munford I ; Eloisa Correia Souza II ; Thabata Alessandra Ramos Caruzo I ; Marina Ba... more ... Veridiana Munford I ; Eloisa Correia Souza II ; Thabata Alessandra Ramos Caruzo I ; Marina Baquerizo ... MAbs against serotypes G1, G2, G3 and G4 (Serotec Rota-MA, Japan) were ... 26. Linhares, AC; Mascarenhas, JDP; Gusmão, RHP; Gabbay, YB; Fialho, AM; Leite, JP (2002). ...
... À Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), pela concessão de bolsa de d... more ... À Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), pela concessão de bolsa de doutorado e pelo apoio financeiro a esta pesquisa. Page 8. CONTINUE A NADAR.... Dory Filme: Procurando Nemo Walt Disney Pictures Page 9. ...
Revista da Sociedade Brasileira de Medicina Tropical, 2007
A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-ye... more A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-year-old children with diarrhea, were used for this study. Molecular characterization of the G and P rotavirus genotypes was performed using the reverse transcriptase polymerase chain reaction. The following results were obtained for the P genotypes: P[8] (17.1%), P[1] (4.9%), P[4] (3.3%), P[6, M37] (2.4%) and mixtures (27.6%). The P[1]+P[8] mixture was found in 19.5% of the samples. For the G genotypes, the results were: G1 (25.2%), G5 (13.8%), G2 (2.5%), G4 (2.5%), G9 (0.8%) and mixtures (41.5%). G1+G5 was the mixture most frequently found (12.1%). Our results showed unusual combinations such as P[1]G5 and P[1]+P[8]G5. The high percentage of mixtures and unusual combinations containing mixtures of human and animal rotavirus genotypes strongly suggests the possibility of gene reassortment and interspecies transmission. RESUMO Um total de 123 amostras fecais de crianças de 0 a 2 anos com diarréia, coletadas em Teresina, Piauí, entre 1994 e 1996 foi utilizada neste estudo. Para a caracterização molecular dos genótipos G e P de rotavírus, foram realizadas as reações de transcriptase reversa e reação em cadeia pela polimerase. Os seguintes resultados foram obtidos para o A alta porcentagem de misturas e as combinações não usuais contendo misturas de genótipos de rotavirus humanos e animais sugerem fortemente a possibilidade de rearranjo genético e transmissão interspecies.
Genetics and Molecular Biology, 2014
The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) b... more The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) but later in many others, led to striking observations on the association of molecular defects and patients' clinical phenotypes. For example, patients with syndromes resulting from defective nucleotide excision repair (NER) or translesion synthesis (TLS) present high levels of skin cancer in areas exposed to sunlight. However, some defects in NER also lead to more severe symptoms, such as developmental and neurological impairment and signs of premature aging. Skin cancer in XP patients is clearly associated with increased mutagenesis and genomic instability, reflecting the defective repair of DNA lesions. By analogy, more severe symptoms observed in NER-defective patients have also been associated with defective repair, likely involving cell death after transcription blockage of damaged templates. Endogenously induced DNA lesions, particularly through oxidative stress, have been identified as responsible for these severe pathologies. However, this association is not that clear and alternative explanations have been proposed. Despite high levels of exposure to intense sunlight, patients from tropical countries receive little attention or care, which likely also reflects the lack of understanding of how DNA damage causes cancer and premature aging.
Memorias Do Instituto Oswaldo Cruz, Dec 1, 2007
The purpose of this study was to characterize astrovirus in faecal samples collected from childre... more The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets: EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) with persistent diarrhea, and 9/55 (16.3%) from control individuals. No data was available for 15/40 (37.5%) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6%) were positive: 12/158 (7.6%) from individuals with acute diarrhea and 6/29 (20.7%) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2%) were characterized as human astrovirus type-1 (HAstV-1), two (3.0%) as HAstV-2, two (3.0%) as HAstV-3, and three (4.5%) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5%) was characterized as HAstV-1, six (33.3%) as HAstV-2, and one (5.5%) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected.
Memórias do Instituto Oswaldo Cruz, 2015
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral struct... more Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for ...
Eur J Clin Microbiol Infect D, 2003
Memórias do Instituto Oswaldo Cruz, 2015
Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral struct... more Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
A study was accomplished to determine the occurrence of rotavirus in feces of calves, in the Stat... more A study was accomplished to determine the occurrence of rotavirus in feces of calves, in the State of São Paulo, Brazil. A total of 375 samples of feces from calves 1 to 45 day-old were collected. The animals belonged to farms situated in six counties of the northeast region of the State. One hundred and ninety tree out of these samples belonged to animals with a clinical picture of diarrhea and 182 were obtained from clinically healthy animals. The techniques used for the detection of rotavirus were the enzyme immunoassay (EIA) and the polyacrilamide gel electrophoresis (PAGE). By the use of EIA, 11.2% (42/375) of the samples were positive for rotavirus. Among the samples of diarrheic calves, 15% (29/193) were found to be positive for rotavirus, whereas 7.1 (13/182) positive samples were obtained from clinically healthy animals. The PAGE test presented a lower sensitivity than EIA, since from the 42 positive samples in EIA, only 36 presented an electrophoretical profile characteris...
Journal of Clinical Microbiology
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
Journal of Clinical Microbiology
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
Journal of clinical microbiology, 2000
The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined usi... more The G (VP7) and P (VP4) serotype distribution of Brazilian porcine rotaviruses was determined using reverse transcription-PCR genotyping methods. Common porcine G types G3, G4, and G5 were detected in combination with P types [6] and [7]. The detection of nonporcine G types and unusual G-P combinations and the characterization of an atypical virus indicated that interspecies transmission may contribute to the genetic diversity of porcine rotaviruses.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1996
Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against ... more Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 micrograms/ml and 500 micrograms/ml. respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 micrograms/ml. Virucidal activity was not observed at 62.5 micrograms/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 micrograms/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.