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Papers by Geraldine Guillermo

Human Molecular Genetics, 2010

Introduction: Phyllodes Tumor is a very low frequent breast fibroepithelial neoplasm, representin... more Introduction: Phyllodes Tumor is a very low frequent breast fibroepithelial neoplasm, representing between 0.3 and 0.9 % of breast tumors and around 2.5% of fibroepithelial tumors as a whole. Ap- pears amid 35 and 55 years old. Goal: To present one case of cystosarcoma phyllodes in a postmenopausal, nuliparous woman. Clinical case: Female patient, 54 years old, smoker, postmenopausal, nuliparous,

Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals ... more Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy specimens and serum for anti-H. pylori immunoglobulin G serology were obtained from 42 patients. Biopsy specimens were studied by histology, culture, and reverse transcription PCR (RT-PCR). Oligonucleotide primers specific for H. pylori (16S rRNA, ureA, and cagA) were used to detect bacterial mRNA in gastric biopsy specimens. PCR was performed on DNA from corresponding H. pylori isolates to detect genomic 16S rRNA, ureA, and cagA. Of the 42 patients from whom clinical specimens were obtained, 25 were infected with H. pylori on the basis of both serology and histology or culture (i.e., tissue positive); 13 were negative by serology, histology, and culture; and 4 were positive by serology only. RT-PCR with 16S rRNA primers detected 24 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). RT-PCR with ureA primers detected 16 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). CagA mRNA was detected by RT-PCR in 14 of 25 gastric biopsy specimens in the tissue-positive group and in 0 of 17 gastric biopsy specimens in the tissue-negative group. PCR of genomic DNA for the presence of the cagA gene in the corresponding bacterial isolates correlated absolutely with cagA gene expression in gastric tissue. These results indicate that RT-PCR is a sensitive and specific method for the detection of the presence of H. pylori and the expression of H. pylori genes in human gastric tissue. Detection of H. pylori gene expression in vivo by this approach may contribute to improving the diagnosis and understanding the pathogenesis of H. pylori infections.

... Anz. Vol.12, N 8: 216-224. 15. HOSHI, N.; HASHIMOTO, Y. 1988. Histological and Immunohistoche... more ... Anz. Vol.12, N 8: 216-224. 15. HOSHI, N.; HASHIMOTO, Y. 1988. Histological and Immunohistochemical Studies on the Architecture of Lymph Nodes in Pig. ... Masson et Cie, Paris VI. 18. POPESCO, P. Atlas d'Anatomie topographique de mamiferes domestiques. Vanders Ed. ...

Clinical and Experimental Immunology, 2008

Helicobacter pylori is a major cause of chronic antral gastritis and peptic ulcer disease. Furthe... more Helicobacter pylori is a major cause of chronic antral gastritis and peptic ulcer disease. Further definition is needed of the factors that determine whether infected individuals remain asymptomatic, or ultimately develop ulceration of the mucosa or transformation to malignancy. To explore the possibility that host response to H. pylori may play a role in the outcome of this infection. we have examined humoral and cellular recognition of several H. pylori proteins by seropositive and seronegative persons. A complex mixture of water-extractable cell proteins, which did not include lipopolysaccharide (LPS), was recognized by serum antibodies only in seropositive or infected individuals. IgG from seropositive subjects also bound to urease and to a heat shock protein (hsp)60 that is homologous to the 65-kD mycobacterial heat shock protein, while sera from uninfected individuals were negative. Although antibody responses to these antigens were restricted to seropositive subjects, T cell recognition of the same proteins was found in both seropositive and seronegative subjects. The water extract of H. pylori stimulated peripheral blood mononuclear cells (PBMC) from all subjects, while purified proteins activated lymphocytes of only some seropositive and seronegative subjects. PBMC that were activated by the H. pylori hsp60 did not respond to the autologous human p60 heat shock protein. These results demonstrate that, in contrast to antibody responses, T cell recognition of H. pylori proteins may occur in non-infected persons. In addition, the data suggest that in these subjects, peripheral lymphocytes that are activated by bacterial heat shock proteins do not mediate tissue damage by recognition of human heat shock homologues.

Some models for the parallel organization of quantum reactive computer codes are discussed. The n... more Some models for the parallel organization of quantum reactive computer codes are discussed. The need for articulating the parallelism at different levels is examined and possible solutions are worked out by analyzing the structure of related theoretical and computational approaches. For the reduced dimensionality program, for which the solution of the bound state problem needs little cpu time, a multilevel task farm parallelization over the angle at the upper level and over the propagation at the lower level was found to be appropriate. On the contrary, for the full dimensional method the solution of the bound state problem is time consuming and has to be parallelized. In this case, a different model has been adopted: the calculations of the surface functions relative to a given subset of sectors have been grouped together and the subsets have been distributed for parallel calculation using a single program multiple data model.

Nature, 2010

Ako si ____________________________, amahan ug si ______________________________ , Inahan ni ____... more Ako si ____________________________, amahan ug si ______________________________ , Inahan ni _________________________________ nagpuyo sa ___________________________________, mosulod niining Panunumpa sa Pakigsaad alang sa pagmatuto sa kinabuhi sa among anak.

Human Molecular Genetics, 2010

Introduction: Phyllodes Tumor is a very low frequent breast fibroepithelial neoplasm, representin... more Introduction: Phyllodes Tumor is a very low frequent breast fibroepithelial neoplasm, representing between 0.3 and 0.9 % of breast tumors and around 2.5% of fibroepithelial tumors as a whole. Ap- pears amid 35 and 55 years old. Goal: To present one case of cystosarcoma phyllodes in a postmenopausal, nuliparous woman. Clinical case: Female patient, 54 years old, smoker, postmenopausal, nuliparous,

Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals ... more Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy specimens and serum for anti-H. pylori immunoglobulin G serology were obtained from 42 patients. Biopsy specimens were studied by histology, culture, and reverse transcription PCR (RT-PCR). Oligonucleotide primers specific for H. pylori (16S rRNA, ureA, and cagA) were used to detect bacterial mRNA in gastric biopsy specimens. PCR was performed on DNA from corresponding H. pylori isolates to detect genomic 16S rRNA, ureA, and cagA. Of the 42 patients from whom clinical specimens were obtained, 25 were infected with H. pylori on the basis of both serology and histology or culture (i.e., tissue positive); 13 were negative by serology, histology, and culture; and 4 were positive by serology only. RT-PCR with 16S rRNA primers detected 24 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). RT-PCR with ureA primers detected 16 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). CagA mRNA was detected by RT-PCR in 14 of 25 gastric biopsy specimens in the tissue-positive group and in 0 of 17 gastric biopsy specimens in the tissue-negative group. PCR of genomic DNA for the presence of the cagA gene in the corresponding bacterial isolates correlated absolutely with cagA gene expression in gastric tissue. These results indicate that RT-PCR is a sensitive and specific method for the detection of the presence of H. pylori and the expression of H. pylori genes in human gastric tissue. Detection of H. pylori gene expression in vivo by this approach may contribute to improving the diagnosis and understanding the pathogenesis of H. pylori infections.

... Anz. Vol.12, N 8: 216-224. 15. HOSHI, N.; HASHIMOTO, Y. 1988. Histological and Immunohistoche... more ... Anz. Vol.12, N 8: 216-224. 15. HOSHI, N.; HASHIMOTO, Y. 1988. Histological and Immunohistochemical Studies on the Architecture of Lymph Nodes in Pig. ... Masson et Cie, Paris VI. 18. POPESCO, P. Atlas d'Anatomie topographique de mamiferes domestiques. Vanders Ed. ...

Clinical and Experimental Immunology, 2008

Helicobacter pylori is a major cause of chronic antral gastritis and peptic ulcer disease. Furthe... more Helicobacter pylori is a major cause of chronic antral gastritis and peptic ulcer disease. Further definition is needed of the factors that determine whether infected individuals remain asymptomatic, or ultimately develop ulceration of the mucosa or transformation to malignancy. To explore the possibility that host response to H. pylori may play a role in the outcome of this infection. we have examined humoral and cellular recognition of several H. pylori proteins by seropositive and seronegative persons. A complex mixture of water-extractable cell proteins, which did not include lipopolysaccharide (LPS), was recognized by serum antibodies only in seropositive or infected individuals. IgG from seropositive subjects also bound to urease and to a heat shock protein (hsp)60 that is homologous to the 65-kD mycobacterial heat shock protein, while sera from uninfected individuals were negative. Although antibody responses to these antigens were restricted to seropositive subjects, T cell recognition of the same proteins was found in both seropositive and seronegative subjects. The water extract of H. pylori stimulated peripheral blood mononuclear cells (PBMC) from all subjects, while purified proteins activated lymphocytes of only some seropositive and seronegative subjects. PBMC that were activated by the H. pylori hsp60 did not respond to the autologous human p60 heat shock protein. These results demonstrate that, in contrast to antibody responses, T cell recognition of H. pylori proteins may occur in non-infected persons. In addition, the data suggest that in these subjects, peripheral lymphocytes that are activated by bacterial heat shock proteins do not mediate tissue damage by recognition of human heat shock homologues.

Some models for the parallel organization of quantum reactive computer codes are discussed. The n... more Some models for the parallel organization of quantum reactive computer codes are discussed. The need for articulating the parallelism at different levels is examined and possible solutions are worked out by analyzing the structure of related theoretical and computational approaches. For the reduced dimensionality program, for which the solution of the bound state problem needs little cpu time, a multilevel task farm parallelization over the angle at the upper level and over the propagation at the lower level was found to be appropriate. On the contrary, for the full dimensional method the solution of the bound state problem is time consuming and has to be parallelized. In this case, a different model has been adopted: the calculations of the surface functions relative to a given subset of sectors have been grouped together and the subsets have been distributed for parallel calculation using a single program multiple data model.

Nature, 2010

Ako si ____________________________, amahan ug si ______________________________ , Inahan ni ____... more Ako si ____________________________, amahan ug si ______________________________ , Inahan ni _________________________________ nagpuyo sa ___________________________________, mosulod niining Panunumpa sa Pakigsaad alang sa pagmatuto sa kinabuhi sa among anak.