Francisco Castro Soto | Universidad De Talca (original) (raw)

Uploads

Papers by Francisco Castro Soto

Nature Genetics, 2011

Background: B-cell non-Hodgkin lymphoma represents a diverse group of hematological malignancies,... more Background: B-cell non-Hodgkin lymphoma represents a diverse group of hematological malignancies, of which follicular lymphoma (FL) is one of the most common subtypes. Family and epidemiological studies suggest an important genetic role in the etiology of FL. In recent genome-wide association studies (GWAS) of FL, several genetic susceptibility loci have been identified on chromosome 6p21.33 (rs6457327) and 6p21.32 (rs10484561, rs2647012) in the human leukocyte antigen class I and class II regions. To identify new genetic variants and further elucidate the genetic basis of FL, a meta-analysis was performed of the top 1000 SNPs associated with FL risk from two GWAS in the US, Denmark and Sweden (592 cases, 1541 controls), with independent validation in 107 cases and 681 controls.

Journal of Physical Chemistry C, 2010

Surface-enhanced Raman scattering (SERS) of benzoate recorded on silver electrode have been analy... more Surface-enhanced Raman scattering (SERS) of benzoate recorded on silver electrode have been analyzed on the basis of a charge transfer (CT) enhancement mechanism similar to a resonance Raman (RR) process where a photoinduced electron transfer between a metal and an adsorbate takes place. Given that benzoic acid (BzH) is adsorbed on silver as benzoate anion (Bz -), this resonant CT process implies the transient formation of the corresponding radical dianion (Bz •2-). The dependence of the relative SERS intensities on the electrode potential has been analyzed on the basis of ab initio calculations of the electronic properties of the isolated adsorbate in its ground and excited CT states (Bzand Bz •2-, respectively). Moreover, configuration interaction singles (CIS) calculations have also been carried out for the Ag 2 -benzoate (Ag 2 -Bz -) supermolecule, which shows two excited singlets, namely CT 0 ; 1 B 1 and CT 1 ; 1 A 2 , where the properties of the adsorbate are quite similar to those of the radical dianion of the isolated species. The theoretical calculations predict the selective enhancement of modes 8a and ν s (CO 2 -) under CT resonant conditions in close agreement with the SERS results. This confirms once again the relevance of resonant CT processes in the SERS of aromatic molecules and the usefulness of this technique in the study of very unstable species such as radical anions or dianions of aromatic adsorbates.

Clinica Chimica Acta, 2011

A single midnight serum cortisol (MSC) test has been reported to possess the best sensitivity and... more A single midnight serum cortisol (MSC) test has been reported to possess the best sensitivity and specificity for diagnosing Cushing's syndrome (CS). However, this test requires patient hospitalization, making it costly. This paper aims to compare the hospital budget impact and accuracy of using midnight salivary cortisol (MSVC), as opposed to MSC, in the diagnosis of hypercortisolism. 77 patients with at least two high urinary free cortisol (UFC) values (>360 nmol/24 h) were selected from 611 patients with clinical symptoms of CS. The costs of the method to confirm the diagnosis of hypercortisolism was calculated comparing Option A using MSC (UFCx2, low-dose dexamethasone suppression test [LDDST]) that requires patient hospitalization versus Option B using MSVC (UFCx2, LDDST) in which the evaluation is done outside the Hospital. A budget impact analysis for one year was developed, and a sensitivity analysis in different scenarios was performed. Reproducibility and diagnostic performance of MSVC and MSC were also measured. Salivary cortisol is a sound analytical method for evaluating free serum cortisol due to its classification accuracy, good imprecision, linearity, and stability. AUC(ROC) comparison between MSVC and MSC shows no significant differences. The substitution of the MSC for MSVC in our hospital could save between €16,762 and €132,804 in one year. The use of MSVC in the diagnosis of hypercortisolism can result in a substantial decrease in the budget impact, without losing diagnosis accuracy and reliability, a significant advantage considering the current emphasis on reducing the financial burden of health care.

Journal of Steroid Biochemistry, 1982

During the normal menstrual cycle, the changes in estradiol binding to cytosol and nuclear recept... more During the normal menstrual cycle, the changes in estradiol binding to cytosol and nuclear receptors of ampulla. isthmus and fimbria were analyzed. Cytosol estradiol receptor concentration showed little variation throughout the cycle, but were higher at the periovulatory phases. The concentration of nuclear estradiol receptor markedly varied in isthmus and ampulla; being significantly higher at the late proliferative phase than at the secretory phases. A positive linear correlation between nuclear receptor content and plasma estradiol concentration was observed, while a negative one was found with plasma progesterone.

Revista Espanola De Salud Publica, 2004

Fundamento: El aumento en la frecuencia de tuberculosis (TB) se produce por varios factores: resi... more Fundamento: El aumento en la frecuencia de tuberculosis (TB) se produce por varios factores: resistencias al tratamiento, la infección por el virus de la inmunodeficiencia humana, el paro, la pobreza y los movimientos migratorios. El objetivo del estudio fue conocer la prevalencia de infección tuberculosa en población inmigrante del Área de Salud de Toledo.

PLOS One, 2011

Background: There are several known autosomal genes responsible for Ras/MAPK pathway syndromes, i... more Background: There are several known autosomal genes responsible for Ras/MAPK pathway syndromes, including Noonan syndrome (NS) and related disorders (such as LEOPARD, neurofibromatosis type 1), although mutations of these genes do not explain all cases. Due to the important role played by the mitochondrion in the energetic metabolism of cardiac muscle, it was recently proposed that variation in the mitochondrial DNA (mtDNA) genome could be a risk factor in the Noonan phenotype and in hypertrophic cardiomyopathy (HCM), which is a common clinical feature in Ras/MAPK pathway syndromes. In order to test these hypotheses, we sequenced entire mtDNA genomes in the largest series of patients suffering from Ras/MAPK pathway syndromes analyzed to date (n = 45), most of them classified as NS patients (n = 42).

Quaternary International

Cleaning works in the cave of Las Palomas in Teba (Málaga, Spain), developed by the Guadalteba Co... more Cleaning works in the cave of Las Palomas in Teba (Málaga, Spain), developed by the Guadalteba Consortium, have provided a number of lithic tools and knapping products that may be ascribed to the Mode III technotypological tradition as well as remains of a number of large mammal species typical of MiddleeLate Pleistocene times. Topographic measurements help to place this ancient cave within a karst landform. This discovery opens up new perspectives in the research on the Neanderthal groups that inhabited the valleys of Guadalteba and Turón rivers in the middle basin of the Guadalhorce River, and thus in the southern region of the Iberian Peninsula.

Physical Review Letters, 2011

European Journal of Neuroscience, 2008

Nature Genetics, 2011

Background: B-cell non-Hodgkin lymphoma represents a diverse group of hematological malignancies,... more Background: B-cell non-Hodgkin lymphoma represents a diverse group of hematological malignancies, of which follicular lymphoma (FL) is one of the most common subtypes. Family and epidemiological studies suggest an important genetic role in the etiology of FL. In recent genome-wide association studies (GWAS) of FL, several genetic susceptibility loci have been identified on chromosome 6p21.33 (rs6457327) and 6p21.32 (rs10484561, rs2647012) in the human leukocyte antigen class I and class II regions. To identify new genetic variants and further elucidate the genetic basis of FL, a meta-analysis was performed of the top 1000 SNPs associated with FL risk from two GWAS in the US, Denmark and Sweden (592 cases, 1541 controls), with independent validation in 107 cases and 681 controls.

Journal of Physical Chemistry C, 2010

Surface-enhanced Raman scattering (SERS) of benzoate recorded on silver electrode have been analy... more Surface-enhanced Raman scattering (SERS) of benzoate recorded on silver electrode have been analyzed on the basis of a charge transfer (CT) enhancement mechanism similar to a resonance Raman (RR) process where a photoinduced electron transfer between a metal and an adsorbate takes place. Given that benzoic acid (BzH) is adsorbed on silver as benzoate anion (Bz -), this resonant CT process implies the transient formation of the corresponding radical dianion (Bz •2-). The dependence of the relative SERS intensities on the electrode potential has been analyzed on the basis of ab initio calculations of the electronic properties of the isolated adsorbate in its ground and excited CT states (Bzand Bz •2-, respectively). Moreover, configuration interaction singles (CIS) calculations have also been carried out for the Ag 2 -benzoate (Ag 2 -Bz -) supermolecule, which shows two excited singlets, namely CT 0 ; 1 B 1 and CT 1 ; 1 A 2 , where the properties of the adsorbate are quite similar to those of the radical dianion of the isolated species. The theoretical calculations predict the selective enhancement of modes 8a and ν s (CO 2 -) under CT resonant conditions in close agreement with the SERS results. This confirms once again the relevance of resonant CT processes in the SERS of aromatic molecules and the usefulness of this technique in the study of very unstable species such as radical anions or dianions of aromatic adsorbates.

Clinica Chimica Acta, 2011

A single midnight serum cortisol (MSC) test has been reported to possess the best sensitivity and... more A single midnight serum cortisol (MSC) test has been reported to possess the best sensitivity and specificity for diagnosing Cushing's syndrome (CS). However, this test requires patient hospitalization, making it costly. This paper aims to compare the hospital budget impact and accuracy of using midnight salivary cortisol (MSVC), as opposed to MSC, in the diagnosis of hypercortisolism. 77 patients with at least two high urinary free cortisol (UFC) values (>360 nmol/24 h) were selected from 611 patients with clinical symptoms of CS. The costs of the method to confirm the diagnosis of hypercortisolism was calculated comparing Option A using MSC (UFCx2, low-dose dexamethasone suppression test [LDDST]) that requires patient hospitalization versus Option B using MSVC (UFCx2, LDDST) in which the evaluation is done outside the Hospital. A budget impact analysis for one year was developed, and a sensitivity analysis in different scenarios was performed. Reproducibility and diagnostic performance of MSVC and MSC were also measured. Salivary cortisol is a sound analytical method for evaluating free serum cortisol due to its classification accuracy, good imprecision, linearity, and stability. AUC(ROC) comparison between MSVC and MSC shows no significant differences. The substitution of the MSC for MSVC in our hospital could save between €16,762 and €132,804 in one year. The use of MSVC in the diagnosis of hypercortisolism can result in a substantial decrease in the budget impact, without losing diagnosis accuracy and reliability, a significant advantage considering the current emphasis on reducing the financial burden of health care.

Journal of Steroid Biochemistry, 1982

During the normal menstrual cycle, the changes in estradiol binding to cytosol and nuclear recept... more During the normal menstrual cycle, the changes in estradiol binding to cytosol and nuclear receptors of ampulla. isthmus and fimbria were analyzed. Cytosol estradiol receptor concentration showed little variation throughout the cycle, but were higher at the periovulatory phases. The concentration of nuclear estradiol receptor markedly varied in isthmus and ampulla; being significantly higher at the late proliferative phase than at the secretory phases. A positive linear correlation between nuclear receptor content and plasma estradiol concentration was observed, while a negative one was found with plasma progesterone.

Revista Espanola De Salud Publica, 2004

Fundamento: El aumento en la frecuencia de tuberculosis (TB) se produce por varios factores: resi... more Fundamento: El aumento en la frecuencia de tuberculosis (TB) se produce por varios factores: resistencias al tratamiento, la infección por el virus de la inmunodeficiencia humana, el paro, la pobreza y los movimientos migratorios. El objetivo del estudio fue conocer la prevalencia de infección tuberculosa en población inmigrante del Área de Salud de Toledo.

PLOS One, 2011

Background: There are several known autosomal genes responsible for Ras/MAPK pathway syndromes, i... more Background: There are several known autosomal genes responsible for Ras/MAPK pathway syndromes, including Noonan syndrome (NS) and related disorders (such as LEOPARD, neurofibromatosis type 1), although mutations of these genes do not explain all cases. Due to the important role played by the mitochondrion in the energetic metabolism of cardiac muscle, it was recently proposed that variation in the mitochondrial DNA (mtDNA) genome could be a risk factor in the Noonan phenotype and in hypertrophic cardiomyopathy (HCM), which is a common clinical feature in Ras/MAPK pathway syndromes. In order to test these hypotheses, we sequenced entire mtDNA genomes in the largest series of patients suffering from Ras/MAPK pathway syndromes analyzed to date (n = 45), most of them classified as NS patients (n = 42).

Quaternary International

Cleaning works in the cave of Las Palomas in Teba (Málaga, Spain), developed by the Guadalteba Co... more Cleaning works in the cave of Las Palomas in Teba (Málaga, Spain), developed by the Guadalteba Consortium, have provided a number of lithic tools and knapping products that may be ascribed to the Mode III technotypological tradition as well as remains of a number of large mammal species typical of MiddleeLate Pleistocene times. Topographic measurements help to place this ancient cave within a karst landform. This discovery opens up new perspectives in the research on the Neanderthal groups that inhabited the valleys of Guadalteba and Turón rivers in the middle basin of the Guadalhorce River, and thus in the southern region of the Iberian Peninsula.

Physical Review Letters, 2011

European Journal of Neuroscience, 2008