Rodrigo Lingnau | UTFPR - UNIVERSIDADE TECNOLOGICA FEDERAL DO PARANÁ (original) (raw)

Papers by Rodrigo Lingnau

Environmental Monitoring and Assessment, 2021

The Campos de Palmas Wildlife Refuge (RVS-CP) is a full protection conservation unit (CU) formed ... more The Campos de Palmas Wildlife Refuge (RVS-CP) is a full protection conservation unit (CU) formed by private properties. The present study aimed to use the bioindicators Allium cepa L. (cytotoxicity and mutagenicity tests) and Eisenia fetida (avoidance test) to assess the quality of surface water of the Chopim River within the RVS-CP area and its surroundings during the four seasons of the year. To do so, water samples were collected at five points, four inside the RVS-CP area and a fifth point outside thereof. Samples from all sampling points had cytotoxic effect on A. cepa in at least one season of the year. Such a finding may be related to inadequate management practices (without land-use control) in the areas surrounding the sampling points such as forestry, native fields, pastures, agriculture, and housing areas. As for the animal bioindicator (E. fetida), only points 1 (in the winter) and 5 (in the autumn) were toxic. Concerning mutagenicity, points 1 and 4 (in the spring), 1 and 2 (in the summer), and 3 (in the autumn) showed mutagenic effect on A. cepa meristematic cells, therefore only within the RVS-CP area. Overall, these results show that biomonitoring can be an ally of the residents of the RVS-CP area in controlling management practices, aiming to bring together economic support and conservation of resources, especially water.

Sphaenorhynchus caramaschii sp. nov. Sphaenorhynchus surdus... more Sphaenorhynchus caramaschii sp. nov. Sphaenorhynchus surdus: BERTOLUCI AND RODRIGUES, 2002; POMBAL JR. AND HADDAD, 2005 Holotype. CFBH 2222, an adult male collected in a permanent pond at the Fazenda São Luís (24 º 21 ' 30 " S, 48 º 44 ' 35 " W; 910 m. altitude), municipality of Ribeirão Branco, state of São Paulo, southeastern Brazil, by Célio F. B. Haddad and José P. Pombal Jr. on 27 November 1993 (Figure 4). Paratopotypes. CFBH 2219 - 21; 2223 adult males collected with the holotype. All the remaining paratypes were collected in the same locality of the holotype (Fazenda São Luís, Ribeirão Branco, São Paulo), but in different dates. CFBH 194 adult male collected on 11 October 1985 by A. J. Cardoso, M. Gordo, M. Martins, J. P. Pombal Jr., and C. F. B. Haddad; CFBH 267 adult male collected on 26 January 1989 by C. F. B. Haddad and J. P. Pombal Jr.; CFBH 370 adult male collected on 27 December 1987 by C. F. B. Haddad, M. Gordo, and J. P. Pombal Jr.; CFBH 1777 adult male collected on 7 December 1992 by C. F. B. Haddad and J. P. Pombal Jr.; CFBH 2210 adult male collected on 8 October 1993 by R. P. Bastos, O. C. Oliveira, and J. P. Pombal Jr.; CFBH 2285 - 94 adult males and a female (CFBH 2287) collected on 8 February 1993 by R. P. Bastos, O. C. Oliveira, and J. P. Pombal Jr.; CFBH 2313 adult male collected on 15 January 1994 by R. P. Bastos and C. F. B. Haddad; CFBH 6875 - 78; 6933 - 37 adult males and one female (CFBH 6933) collected on 14 January 2004 by C. F. B. Haddad, C. P. A. Prado, and L. O. M. Giasson; CFBH 9583; 11285 adult males collected on 14 January 2005 by C. F. B. Haddad, J. Alexandrino, M. Guimarães, and M. Gridi-Papp. In total there are 31 paratypes, of which 29 are males and two are females. Diagnosis. Sphaenorhynchus caramaschii is an intermediate size species for the genus (Figure 5) and is characterized by the following combination of characters: (1) absence of external tympanum; (2) snout from truncate to slightly mucronate in dorsal view and protrudin [...]

Sphaenorhynchus surdus (Cochran, 1953) Hyla aurantiaca surda</i&gt... more Sphaenorhynchus surdus (Cochran, 1953) Hyla aurantiaca surda Cochran, 1953 — Name-bearing type: holotype by original designation, UMMZ 106736, adult male according to original description, SVL 28 mm. – Type locality: "Curitiba, Paraná", Brazil. – Paratypes according to original description – UMMZ 104115, adult male collected with the holotype; UMMZ 104116 A – C, adult males collected in another site in the municipality of Curitiba, state of Paraná, Brazil. Herpetologica, 8: 112. Other chresonyms: Hyla aurantiaca: BOULENGER, 1888 Sphoenohyla surda: GOIN, 1957 Sphaenorhynchus surda: GORHAM, 1974 Sphoenorhynchus surdus: BOKERMANN, 1966: 45 Holotype. MZUM 106736. Adult male. SVL 28 mm, collected at the municipality of Curitiba (approximately 25 ° 25 ' S; 49 ° 16 ' O), state of Paraná, Brazil (Figure 1). Diagnosis [as presented in Cochran (1953)] – No external tympanum; interorbital diameter twice the width of the upper eyelid; a dark dorsolateral line from snout almost to groin. Description of Holotype. Provided by Cochran (1953). Advertisement call. Adult males of S. surdus call from the floating vegetation, generally in the deepest portion of temporary or permanent ponds (Figure 2). The advertisement call was described in words by Cochran (1953) as "hitting resonant rocks together quickly 4 or 5 times". The advertisement call of S. surdus, recorded all over its distribution (municipalities of São Bento do Sul, Lebon Régis, Ponte Serrada, Urubici, Lages, and Lontras, all in the state of Santa Catarina, and municipality of São José dos Ausentes, state of Rio Grande do Sul), but in the type locality, has from 18 to 22 notes, ranging from 1.34 ± 0.13 kHz (range: 0.98 – 1.54; n = 27 notes; 1 male) to 3.41 ± 0.17 kHz (range: 3.17 – 3.85; n = 27 notes; 1 male). The mean dominant frequency is 2.29 ± 0.03 kHz (range: 2.24 – 2.37; n = 27 notes; 1 male). The duration of the call is about 1.7 seconds, but it depends on the number [...]

FIGURE 6. Holotype of Sphaenorhynchus caramaschii sp. nov. (CFBH 2222; adult male) in dorsal (A) ... more FIGURE 6. Holotype of Sphaenorhynchus caramaschii sp. nov. (CFBH 2222; adult male) in dorsal (A) and lateral (B) views of head; and ventral views of hand (C) and foot (D).

Anais da Academia Brasileira de Ciências

Our objective was to describe and compare the diet of endemic anurans of the Atlantic Forest, as ... more Our objective was to describe and compare the diet of endemic anurans of the Atlantic Forest, as well as to evaluate the infl uence of biotic factors on the trophic ecology of the species. We conducted the study in Mananciais da Serra (Piraquara-PR), a transition region between mixed and dense ombrophilous forest, between January 2019

Environmental Science and Pollution Research, 2021

Animals have a long history of assessing ecosystem responses to environmental disturbances, and a... more Animals have a long history of assessing ecosystem responses to environmental disturbances, and amphibians stand out for presenting themselves as good animal model and bioindicators of environmental quality. The main purpose of the present work was to investigate the cellular effects of contamination of waters of the Marrecas River, located in the southwest of the state of Paraná, Brazil. Therefore, the objective of the present study was to investigate and monitor the cytotoxic and mutagenic effect, with bullfrog tadpoles, and to discuss these effects with land use along this hydrographic basin. Mutagenic effects were determined by micronucleus assay, and cytotoxicity by other nuclear changes, such as segmented cells, binucleated cells, cells with buds and reniform cells. Water samples were obtained at nine sites along the Marrecas River, covering areas with rural and urban hydrological contribution. For each site, four samples were collected, along the years 2017 and 2018, encompassing the four seasons (summer, autumn, winter, and spring). The results showed mutagenic and cytotoxic effect in four sampling sites, and only cytotoxic effect in other four sites. These effects may be due, possibly, to the use of different agrochemicals across the hydrographic basin region, which have predominant hydrological contributions from crops. Data of this study indicate the presence of cytotoxic and mutagenic contaminants in the waters of the Marrecas River, which can generate environmental problems on the river fauna/flora, and can also affect the local population health.

Phyllomedusa: Journal of Herpetology, 2004

Herein, we describe the vocal repertoire of Hyla werneri. We found four distinct vocalizations: a... more Herein, we describe the vocal repertoire of Hyla werneri. We found four distinct vocalizations: advertisement call, aggressive call, distress call, and a mixed call. The advertisement call was the most frequent vocalization, with one to 23 notes. Aggressive calls had only one note, but with longer duration than notes of advertisement calls. One individual emitted four distress calls, and these showed a great variation. The mixed calls are formed by the emission of an aggressive call followed by five to 23 notes similar to the advertisement call, probably a strategy to partition the attractive and aggressive functions in different notes on the same call. We suggest that the mixed calls of H. werneri could be a graded aggressive call system, already found in other anurans, which allows males to gradually reduce the number of attractive elements while increasing the aggressiveness of the call.

acta ethologica, 2016

Acoustically active animals may show long-and short-term adaptations in acoustic traits for copin... more Acoustically active animals may show long-and short-term adaptations in acoustic traits for coping with ambient noise. Given the key role of calls in anurans' life history, long-and short-term adaptations are expected in species inhabiting noisy habitats. However, to disentangle such adaptations is a difficult task, incipiently addressed for Neotropical frogs. We investigated if males of a stream-breeding frog (Crossodactylus schmidti) adjust call traits according to the background noise, and if the signal-to-noise ratio (SNR) varies between call harmonics and along call notes. We measured sound pressure levels of calls and noise in the field and used a fine-scale acoustic analysis to describe the signal and noise structure and test for noise-related call adjustments. The multi-note harmonic call of C. schmidti greatly varied in the spectral structure, including a trend for increasing note amplitude along the call, a wide frequency bandwidth of the 2nd harmonic, a minor call frequency modulation due to a trend for increasing note frequency within the same harmonic, and a major call frequency modulation due to the variable location of the dominant harmonic along the call. Calls had significantly higher frequencies than the noise at the range of the 1st and the 2nd call harmonics, and significantly louder sound pressure than the noise at the range of all harmonics. Males emitted the majority of call notes showing positive SNR, and though males also emitted some notes with negative SNR, when a given harmonic was negative the other harmonics in the same note did not tend to be SNR-negative. Our results indicate that male C. schmidti show short-term acoustic adjustments that make the advertisement call effective for coping with the interference of the stream-generated noise. We suggest that the call spectral plasticity serves for coping with temporary changes in the background noise, whilst we also discuss the possibility that the redundant, harmonic-structured call may have evolved to diminish masking interference on the acoustic signal by the background noise. This is the first study to uncouple noise-related acoustic adjustments and putative long-term acoustic adaptations for a Hylodidae, providing insights on behavioral plasticity and signal evolution of stream-breeding frogs.

Herpetologica, 2016

Animal activity patterns can be influenced by selection pressures from local environmental condit... more Animal activity patterns can be influenced by selection pressures from local environmental conditions that might fluctuate at different temporal scales. Although studies with frog species and local populations can suggest how variation of environmental factors affects activity patterns, this topic has received little coverage for Neotropical diurnal frogs that inhabit streams, particularly for subtropical species. We described the temporal patterns of calling activity of Schmidt's Spinythumb Frogs (Crossodactylus schmidti) in southern Brazil and investigated the role of environmental factors on this activity. We recorded local environmental variables and monitored calling activity from October 2011 to September 2012, by counting the number of calling males and the number of emitted calls at hourly intervals throughout the day. Male frogs engaged in calling activity year-round, despite the local climatic seasonality. There was no seasonality in the number of calling males or in the number of calls; moreover, variation in photoperiod did not affect the duration of the calling activity throughout the year. Crossodactylus schmidti showed a predominantly diurnal calling activity, with a spike in the early morning followed by a gradual increase in activity during daytime hours, and a subsequent decrease in activity at dusk. Environmental factors had varying effects on calling activity: (1) monthly activity was positively associated with accumulated rainfall and air temperature; (2) early morning activity was greater when air temperatures were higher; and, (3) daytime calling activity was positively associated with light intensity. Whereas our results do not preclude the effect of other environmental factors in the calling behavior of male C. schmidti, accumulated rainfall, air temperature, and light intensity appear to be abiotic regulators of this activity at the analyzed temporal scales. We discuss the biological implications of these environmental factors in the calling activity and reproduction of C. schmidti, and compare the observed patterns with those of other taxa of diurnal stream-breeding frogs.

South American Journal of Herpetology, 2011

We report new records of Lithobates catesbeianus feral populations in Brazil. Data were based on ... more We report new records of Lithobates catesbeianus feral populations in Brazil. Data were based on fieldwork, natural history collection records, and literature and electronic database searches. Lithobates catesbeianus occurs in 130 municipalities of Brazil, including 55 presented for the first time in this work. Most records are from south and southeastern Brazil in the Atlantic Forest biome with climatic conditions that are favorable to the establishment of bullfrog populations. The wide and possibly expanding distribution of feral L. catesbeianus populations in Brazil poses a major conservation challenge and demands research on the invasion patterns. KEYWORDS. Bullfrogs; distribution; Brazil; feral populations; biological invasion.

PLoS ONE, Feb 24, 2017

Phenotypic traits, such as the frog advertisement call, are generally correlated with interspecif... more Phenotypic traits, such as the frog advertisement call, are generally correlated with interspecific genetic variation, and, as a consequence of strong sexual selection, these behaviors may carry a phylogenetic signal. However, variation in acoustic traits is not always correlated with genetic differences between populations (intraspecific variation); phenotypic plasticity and environmental variables may explain part of such variation. For example, local processes can affect acoustic properties in different lineages due to differences in physical structure, climatic conditions, and biotic interactions, particularly when populations are isolated. However, acoustic traits can be used to test phylogenetic hypotheses. We analyzed the advertisement calls of Dendropsophus elegans males from 18 sites and compared them with those of four closely related congeneric species, in order to test for differences between inter and intraspecific variation. We analyzed 451 calls of 45 males of these five species. Because males from distant sites were grouped together without population congruence, differences found in advertisement calls among individuals were not correlated with phylogeographical clades. Phylogenetic and cluster analyses of the D. elegans clades and those of closely related species grouped all five species into the same topology, as reported by previous molecular and morphological phylogenies. However, the topology of the D. elegans phylogeographical clades did not match the topology previously reported. Acoustic communication in D. elegans seems to be conserved among populations, and the phylogeographical history of the species does not explain the variation among lineages in call properties, despite some congruent phylogenetic signals evident at the species level. Based on molecular clocks retrieved from the literature, it seems that more than 6.5 million years of divergence (late Miocene) are necessary to allow significant changes to occur in the acoustic properties of these treefrog calls, making it possible to recover their phylogenetic history only based on acoustic evidence.

Species distributed across vast continental areas and across major biomes provide unique model sy... more Species distributed across vast continental areas and across major biomes provide unique model systems for studies of biotic diversification, yet also constitute daunting financial, logistic and political challenges for data collection across such regions. The tree frog Dendropsophus minutus (Anura: Hylidae) is a nominal species, continentally distributed in South America, that may represent a complex of multiple species, each with a more limited distribution. To understand the spatial pattern of molecular diversity throughout the range of this species complex, we obtained DNA sequence data from two mitochondrial genes, cytochrome oxidase I (COI) and the 16S rhibosomal gene (16S) for 407 samples of D. minutus and closely related species distributed across eleven countries, effectively comprising the entire range of the group. We performed phylogenetic and spatially explicit phylogeographic analyses to assess the genetic structure of lineages and infer ancestral areas. We found 43 statistically supported, deep mitochondrial lineages, several of which may represent currently unrecognized distinct species. One major clade, containing 25 divergent lineages, includes samples from the type locality of D. minutus. We defined that clade as the D. minutus complex. The remaining lineages together with the D. minutus complex constitute the D. minutus species group. Historical analyses support an Amazonian origin for the D. minutus species group with a subsequent dispersal to eastern Brazil where the D. minutus complex originated. According to our dataset, a total of eight mtDNA lineages have ranges .100,000 km 2. One of them occupies an area of almost one million km 2 encompassing multiple biomes. Our results, at a spatial scale and resolution unprecedented for a Neotropical vertebrate, confirm that widespread amphibian species occur in lowland South America, yet at the same time a large proportion of cryptic diversity still remains to be discovered.

The advertisement call of the South Brazilian hylid Hyla ehrhardti is described for the first tim... more The advertisement call of the South Brazilian hylid Hyla ehrhardti is described for the first time. It consists of a long series of single notes with high repetition rates ranging from 204-264 notes/min. The dominant frequency lies between 2.6 and 2.9 kHz. New records for Hyla ehrhardti significantly extend the distribution of this little known species, which was restricted to the State of Santa Catarina, northward, through Parana State up to Sao Paulo State.

The Proceratophrys bigibbosa species group is characterized by the presence of postocular swellin... more The Proceratophrys bigibbosa species group is characterized by the presence of postocular swellings and absence of hornlike palpebral appendages. A new member of this group was described recently from southern Brazil: Proceratophrys brauni. Its body size is between the smaller Proceratophrys avelinoi and the larger Proceratophrys bigibbosa species, both living in the same region. As the external appearance of these three members of the group is very similar to each other, it is interesting to discover a specific morphological categorization through internal characteristics, such as the cranium’s proportions. In this paper, we report the preliminary results for comparative cranium’s morphological characterization of Proceratophrys bigibbosa species from Brazil using the X-ray computed Microtomography technique through Skyscan 1174 system. Five samples of each three species, i.e., fifteen samples in total, were scanned. The tomographic slice images were reconstructed by SkyScan softwa...

Complex interactions among hosts, pathogens, and the environment affect the vulnerability of amph... more Complex interactions among hosts, pathogens, and the environment affect the vulnerability of amphibians to the emergence of infectious diseases such as chytridiomycosis, caused by Batrachochytrium dendrobatidis (Bd). Boana curupi is a forest-dwelling amphibian endemic to the southern Atlantic Forest of South America, a severely fragmented region. Here, we evaluated whether abiotic factors (including air and water temperature, relative air humidity, and landscape) are correlated with chytrid infection intensity and prevalence in B. curupi. We found individuals infected with Bd in all populations sampled. Prevalence ranged from 25-86%, and the infection burden ranged from 1 to over 130000 zoospore genomic equivalents (g.e.) (mean ± SD: 4913 ± 18081 g.e.). The infection load differed among populations and was influenced by forest cover at scales of 100, 500, and 1000 m, with the highest infection rates recorded in areas with a higher proportion of forest cover. Our results suggest that...