Camacho Garduño | Universidad Tecnológica del Mar de Tamaulipas Bicentenario (original) (raw)

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Research paper thumbnail of The Effect of Aspartame on Rat Brain Xenobiotic-Metabolizing Enzymes

Human & Experimental Toxicology, 2006

This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I ... more This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I metabolizing enzymes (cytochrome P450 (CYP)) in rat brain. Wistar rats were treated by gavage with ASP at daily doses of 75 and 125 mg/kg body weight for 30 days. Cerebrum and cerebellum were used to obtain microsomal fractions to analyse activity and protein levels of seven cytochrome P450 enzymes. Increases in activity were consistently found with the 75 mg/kg dose both in cerebrum and cerebellum for all seven enzymes, although not at the same levels: CYP 2E1-associated 4-nitrophenol hydroxylase (4-NPH) activity was increased 1.5-fold in cerebrum and 25-fold in cerebellum; likewise, CYP2B1-associated penthoxyresorufin O-dealkylase (PROD) activity increased 2.9-and 1.7-fold respectively, CYP2B2-associated benzyloxyresorufin O-dealkylase (BROD) 4.5-and 1.1fold, CYP3A-associated erythromycin N -demethylase (END) 1.4-and 3.3-fold, CYP1A1-associated ethoxyresorufin O-deethylase (EROD) 5.5-and 2.8-fold, and CYP1A2associated methoxyresorufin O-demethylase (MROD) 3.7and 1.3-fold. Furthermore, the pattern of induction of CYP immunoreactive proteins by ASP paralleled that of 4-NHP-, PROD-, BROD-, END-, EROD-and MROD-related activities only in the cerebellum. Conversely, no differences in CYP concentration and activity were detected in hepatic microsomes of treated animals with respect to the controls, suggesting a brain-specific response to ASP treatment. Human & Experimental Toxicology (2006) 25, 453 Á 459

Research paper thumbnail of Oxidative stress, cytoxicity, and cell mortality induced by nano-sized lead in aqueous suspensions

Chemosphere, 2011

This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid p... more This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6h after incubation aqueous suspensions bearing nano-sized PbO(2), soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1 nmol TBARS mg protein(-1), respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO(2) led to cell-growth inhibition values (%) ca. ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however.

Research paper thumbnail of Constitucional

CONCLUSIONES 144 BIBLIOGRAFÍA 147 INTRODUCCIÓN En el Sistema Jurídico Mexicano, las autoridades s... more CONCLUSIONES 144 BIBLIOGRAFÍA 147 INTRODUCCIÓN En el Sistema Jurídico Mexicano, las autoridades sólo pueden hacer lo que la Constitución les permite y faculta. Tomando en cuenta que el principio de Supremacía Constitucional nos señala que la Constitución Política de los Estados Unidos Mexicanos, es la norma de mayor jerarquía en nuestro país, tenemos que los actos de autoridad que de ella emanen, deben apegarse a sus mandatos y no contravenirla, de lo contrario estos actos serán susceptibles de ser anulados por violar a la Constitución.

Research paper thumbnail of The Effect of Aspartame on Rat Brain Xenobiotic-Metabolizing Enzymes

Human & Experimental Toxicology, 2006

This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I ... more This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I metabolizing enzymes (cytochrome P450 (CYP)) in rat brain. Wistar rats were treated by gavage with ASP at daily doses of 75 and 125 mg/kg body weight for 30 days. Cerebrum and cerebellum were used to obtain microsomal fractions to analyse activity and protein levels of seven cytochrome P450 enzymes. Increases in activity were consistently found with the 75 mg/kg dose both in cerebrum and cerebellum for all seven enzymes, although not at the same levels: CYP 2E1-associated 4-nitrophenol hydroxylase (4-NPH) activity was increased 1.5-fold in cerebrum and 25-fold in cerebellum; likewise, CYP2B1-associated penthoxyresorufin O-dealkylase (PROD) activity increased 2.9-and 1.7-fold respectively, CYP2B2-associated benzyloxyresorufin O-dealkylase (BROD) 4.5-and 1.1fold, CYP3A-associated erythromycin N -demethylase (END) 1.4-and 3.3-fold, CYP1A1-associated ethoxyresorufin O-deethylase (EROD) 5.5-and 2.8-fold, and CYP1A2associated methoxyresorufin O-demethylase (MROD) 3.7and 1.3-fold. Furthermore, the pattern of induction of CYP immunoreactive proteins by ASP paralleled that of 4-NHP-, PROD-, BROD-, END-, EROD-and MROD-related activities only in the cerebellum. Conversely, no differences in CYP concentration and activity were detected in hepatic microsomes of treated animals with respect to the controls, suggesting a brain-specific response to ASP treatment. Human & Experimental Toxicology (2006) 25, 453 Á 459

Research paper thumbnail of Oxidative stress, cytoxicity, and cell mortality induced by nano-sized lead in aqueous suspensions

Chemosphere, 2011

This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid p... more This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6h after incubation aqueous suspensions bearing nano-sized PbO(2), soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1 nmol TBARS mg protein(-1), respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO(2) led to cell-growth inhibition values (%) ca. ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however.

Research paper thumbnail of Constitucional

CONCLUSIONES 144 BIBLIOGRAFÍA 147 INTRODUCCIÓN En el Sistema Jurídico Mexicano, las autoridades s... more CONCLUSIONES 144 BIBLIOGRAFÍA 147 INTRODUCCIÓN En el Sistema Jurídico Mexicano, las autoridades sólo pueden hacer lo que la Constitución les permite y faculta. Tomando en cuenta que el principio de Supremacía Constitucional nos señala que la Constitución Política de los Estados Unidos Mexicanos, es la norma de mayor jerarquía en nuestro país, tenemos que los actos de autoridad que de ella emanen, deben apegarse a sus mandatos y no contravenirla, de lo contrario estos actos serán susceptibles de ser anulados por violar a la Constitución.